pycom15g32260

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009921
Physical Location & Seq
Reverse (-)
31213959 .. 31218461
4503 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1410 bp
ATGAACCGGAAATCTAGTTGTGCTATTTGTGAGAATTCAAATCTTGCTTCTATCTGTGCTGTTTGTGTGAATTACAGATTAAATGAGTATAACAATTCGTTAAAGGTACTGAAGAGTCGTCGTGATTCATTGTATTCAAGATTGACTGAAGTGCTTGTCGCAAAGGGTAAGGCTGATGATCAAATAAATTGGAGAGTGCTTCAAAATGAGAAGCTTGTGAGATTAAGGGAAAAGCTCCAGTGTAATACAGAACAGCTTGTGCAAGGGAAGGCTAAGATTGAGAAGACATCCTATGACCTCAAAGTCAAATATGGGGTGCTTGATTCGGCCCTCTCTGTGTTGAGAAAGAATCGTGTGGAGCAATTGGATAAGTTCTATCCTAACCTTATATGCACTCAGAACTTAGGGCATATGGCCATTACCTCTGAACGCCTTCATAAGCAGTCAGTAGTTATAAAACAAATATGCAAATTATTTCCCCAACGTCGGGTGATTGTAGATGAAAAGAAGAATGATGGATCTGGTGGTCTATATGATCAAATTTGCAATGCATGCTTACCAAGAGGGCTAGATCCACACACTGTTCCATCAGAAGAGCTTGCTGCTTCCCTGGGGTACATGGTTCAACTCCTGAATCTTGTTGTTCATAACTTAGCTGCTCCAGCACTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGGATATGGCAAAGAGATTCTTATTGGGATGCATCCCCTTCTTCTCGGAGCAATGAATATCCCCTCTTTATACCACGCCAAAATTATTGCTCCAGTAGCGGGGAAAATTCTTGGTCTGACAGGAGCTCAAGTAATAATTTTGGTGTTGCTTCAATAGAATCCGTGAGAAAACCGCGTCTGGATTCTTCTGGTGGTAGCTTTAATTATTCTTCTGCTTCTGCACACAGTGTTGAAACGCACAAAGATTTGCAGAGAGGGATTTCACTTCTTAAGAAAAGTGTGGCATGCATTACAGCGTATTGTTATAACTCACTATGTTTAGATGTCCCTTCTGAGGTATCTACATTTGAAGCATTTGCTAAATTGTTGGCAACATTATCTTCTTCCAAGGAAGTTCATTCTGTGTTCTCGTTGAAAATGGCTTGCTCGAGGTCATGTAAGCAAGTTAAACAATTAAACAAGTCTGTGTGGAATGTGAATTCTGCCATATCGTCAACCACTCTGTTGGATAGCGCACATGCAATGGCACTAACGAAAAACTTAAATGAAAACAACCTTCTTTGTTCCACTGAGTTGTCTGAAGGGAAGAATGAATCCCTTATTGAAGGATGGGATCTTGTGGAACATCCGACTTTTCCCCCTCCACCATCACAGTCCGAGGATATTGAGCATTGGACTCGAGCCATGATCATCGATGCTAAAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

470

Amino Acids

52.33

Weight (kDa)

8.84

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 7 - 364 4.1e-83 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 1005
AasI GACNNNNNNGTC 1 cut(s) 302
AccII CGCG 1 cut(s) 874
AciI CCGC 3 cut(s) 686, 798, 872
AclWI GGATC 3 cut(s) 526, 566, 1320
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 4 cut(s) 34, 540, 805, 1177
AcuI CTGAAG 3 cut(s) 131, 168, 1299
AdeI CACNNNGTG 1 cut(s) 926
AfaI GTAC 2 cut(s) 108, 617
AfiI CCNNNNNNNGG 4 cut(s) 486, 487, 798, 1033
AflII CTTAAG 1 cut(s) 968
AgsI TTSAA 9 cut(s) 39, 138, 203, 626, 852, 932, 1049, 1114, 1304
AjnI CCWGG 1 cut(s) 609
AluBI AGCT 7 cut(s) 214, 235, 256, 598, 656, 825, 897
AluI AGCT 7 cut(s) 214, 235, 256, 598, 656, 825, 897
Alw21I GWGCWC 1 cut(s) 827
AlwI GGATC 3 cut(s) 526, 566, 1320
Ama87I CYCGRG 2 cut(s) 1126, 1377
AoxI GGCC 2 cut(s) 327, 414
ApeKI GCWGC 2 cut(s) 602, 656
ApoI RAATTY 4 cut(s) 34, 540, 805, 1177
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 1214
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 1126, 1377
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 827
BbvI GCAGC 2 cut(s) 589, 643
BccI CCATC 4 cut(s) 509, 595, 1302, 1354
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 1 cut(s) 611
BclI TGATCA 3 cut(s) 178, 535, 1386
BfaI CTAG 2 cut(s) 15, 569
BfrI CTTAAG 1 cut(s) 968
BisI GCNGC 2 cut(s) 603, 657
BlsI GCNGC 2 cut(s) 604, 658
Bme1390I CCNGG 1 cut(s) 611
BmeT110I CYCGRG 2 cut(s) 1126, 1377
BmgT120I GGNCC 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 611
BmsI GCATC 3 cut(s) 718, 740, 1384
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 3 cut(s) 221, 645, 775
BpuEI CTTGAG 1 cut(s) 811
Bsa29I ATCGAT 1 cut(s) 1392
BsaJI CCNNGG 4 cut(s) 609, 610, 1086, 1356
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 814, 844
Bsc4I CCNNNNNNNGG 4 cut(s) 486, 487, 798, 1033
Bse1I ACTGG 2 cut(s) 238, 792
Bse3DI GCAATG 3 cut(s) 553, 757, 1227
BseBI CCWGG 1 cut(s) 611
BseCI ATCGAT 1 cut(s) 1392
BseDI CCNNGG 4 cut(s) 609, 610, 1086, 1356
BseGI GGATG 5 cut(s) 287, 731, 733, 1313, 1324
BseLI CCNNNNNNNGG 4 cut(s) 486, 487, 798, 1033
BseMI GCAATG 3 cut(s) 553, 757, 1227
BseMII CTCAG 4 cut(s) 410, 690, 1023, 1260
BseNI ACTGG 2 cut(s) 238, 792
BseXI GCAGC 2 cut(s) 589, 643
BsgI GTGCAG 1 cut(s) 903
Bsh1236I CGCG 1 cut(s) 874
BshFI GGCC 2 cut(s) 329, 416
BshVI ATCGAT 1 cut(s) 1392
BsiHKAI GWGCWC 1 cut(s) 827
BsiHKCI CYCGRG 2 cut(s) 1126, 1377
BsiSI CCGG 1 cut(s) 7
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 4 cut(s) 486, 487, 798, 1033
BsmFI GGGAC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 329, 416
BsoBI CYCGRG 2 cut(s) 1126, 1377
Bsp1286I GDGCHC 1 cut(s) 827
Bsp143I GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
BspACI CCGC 3 cut(s) 686, 798, 872
BspANI GGCC 2 cut(s) 329, 416
BspCNI CTCAG 4 cut(s) 409, 689, 1024, 1261
BspDI ATCGAT 1 cut(s) 1392
BspFNI CGCG 1 cut(s) 874
BspPI GGATC 3 cut(s) 526, 566, 1320
BspQI GCTCTTC 1 cut(s) 588
BspTI CTTAAG 1 cut(s) 968
BsrDI GCAATG 3 cut(s) 553, 757, 1227
BsrI ACTGG 2 cut(s) 238, 792
BssECI CCNNGG 4 cut(s) 609, 610, 1086, 1356
BssMI GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
BssT1I CCWWGG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 3 cut(s) 583, 926, 1353
Bst6I CTCTTC 2 cut(s) 107, 588
BstAFI CTTAAG 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 552
BstC8I GCNNGC 4 cut(s) 553, 600, 985, 1123
BstDEI CTNAG 7 cut(s) 273, 396, 403, 652, 676, 1032, 1269
BstF5I GGATG 5 cut(s) 287, 731, 733, 1313, 1324
BstFNI CGCG 1 cut(s) 874
BstHHI GCGC 1 cut(s) 1214
BstKTI GATC 6 cut(s) 181, 521, 538, 574, 1315, 1389
BstMBI GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
BstMWI GCNNNNNNNGC 3 cut(s) 552, 662, 795
BstNI CCWGG 1 cut(s) 611
BstNSI RCATGY 3 cut(s) 555, 987, 1220
BstSCI CCNGG 1 cut(s) 609
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 2 cut(s) 589, 643
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 3 cut(s) 518, 571, 1312
BstXI CCANNNNNNTGG 1 cut(s) 1204
BstYI RGATCY 3 cut(s) 518, 571, 1312
Bsu15I ATCGAT 1 cut(s) 1392
BsuRI GGCC 2 cut(s) 329, 416
BsuTUI ATCGAT 1 cut(s) 1392
BtsCI GGATG 5 cut(s) 287, 731, 733, 1313, 1324
BtsIMutI CAGTG 4 cut(s) 245, 579, 931, 1266
Cac8I GCNNGC 4 cut(s) 553, 600, 985, 1123
CfoI GCGC 1 cut(s) 1214
Cfr13I GGNCC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 1392
CseI GACGC 1 cut(s) 863
Csp6I GTAC 2 cut(s) 107, 616
CviAII CATG 6 cut(s) 552, 619, 984, 1134, 1217, 1384
CviQI GTAC 2 cut(s) 107, 616
DdeI CTNAG 7 cut(s) 273, 396, 403, 652, 676, 1032, 1269
DpnI GATC 6 cut(s) 180, 520, 537, 573, 1314, 1388
DpnII GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
DraIII CACNNNGTG 1 cut(s) 926
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 107, 588
EarI CTCTTC 2 cut(s) 107, 588
Ecl136II GAGCTC 1 cut(s) 825
Eco130I CCWWGG 1 cut(s) 1086
Eco24I GRGCYC 1 cut(s) 827
Eco53kI GAGCTC 1 cut(s) 825
Eco57I CTGAAG 3 cut(s) 131, 168, 1299
Eco88I CYCGRG 2 cut(s) 1126, 1377
EcoICRI GAGCTC 1 cut(s) 825
EcoRI GAATTC 2 cut(s) 34, 1177
EcoRII CCWGG 1 cut(s) 609
EcoT14I CCWWGG 1 cut(s) 1086
EcoT22I ATGCAT 3 cut(s) 553, 733, 989
EcoT38I GRGCYC 1 cut(s) 827
ErhI CCWWGG 1 cut(s) 1086
FaeI CATG 6 cut(s) 555, 622, 987, 1137, 1220, 1387
FalI AAGNNNNNCTT 2 cut(s) 703, 735
FaqI GGGAC 1 cut(s) 1010
FatI CATG 6 cut(s) 551, 618, 983, 1133, 1216, 1383
FauI CCCGC 2 cut(s) 679, 791
FauNDI CATATG 1 cut(s) 411
FbaI TGATCA 3 cut(s) 178, 535, 1386
Fnu4HI GCNGC 2 cut(s) 603, 657
FokI GGATG 5 cut(s) 274, 718, 740, 1311, 1320
FriOI GRGCYC 1 cut(s) 827
Fsp4HI GCNGC 2 cut(s) 603, 657
FspBI CTAG 2 cut(s) 15, 569
GlaI GCGC 1 cut(s) 1213
GluI GCNGC 2 cut(s) 603, 657
GsuI CTGGAG 3 cut(s) 221, 645, 775
HaeIII GGCC 2 cut(s) 329, 416
HapII CCGG 1 cut(s) 7
HgaI GACGC 1 cut(s) 863
HhaI GCGC 1 cut(s) 1214
Hin1II CATG 6 cut(s) 555, 622, 987, 1137, 1220, 1387
Hin6I GCGC 1 cut(s) 1212
HinP1I GCGC 1 cut(s) 1212
HincII GTYRAC 1 cut(s) 1194
HindII GTYRAC 1 cut(s) 1194
HindIII AAGCTT 1 cut(s) 212
HpaII CCGG 1 cut(s) 7
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 1194
Hpy188III TCNNGA 4 cut(s) 122, 138, 631, 878
Hpy8I GTNNAC 1 cut(s) 1194
Hpy99I CGWCG 2 cut(s) 123, 489
HpyAV CCTTC 7 cut(s) 262, 443, 747, 1038, 1265, 1274, 1298
HpyCH4III ACNGT 3 cut(s) 583, 926, 1353
HpyCH4IV ACGT 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 3 cut(s) 552, 662, 795
HpyF3I CTNAG 7 cut(s) 273, 396, 403, 652, 676, 1032, 1269
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 6 cut(s) 555, 622, 987, 1137, 1220, 1387
HspAI GCGC 1 cut(s) 1212
Ksp22I TGATCA 3 cut(s) 178, 535, 1386
Kzo9I GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 6 cut(s) 240, 358, 664, 747, 794, 822
Lsp1109I GCAGC 2 cut(s) 589, 643
LweI GCATC 3 cut(s) 718, 740, 1384
MaeI CTAG 2 cut(s) 15, 569
MaeII ACGT 1 cut(s) 484
MalI GATC 6 cut(s) 180, 520, 537, 573, 1314, 1388
MboI GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 3 cut(s) 518, 571, 1312
MhlI GDGCHC 1 cut(s) 827
MlsI TGGCCA 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 2 cut(s) 124, 1369
MmeI TCCRAC 2 cut(s) 1185, 1352
Mox20I TGGCCA 1 cut(s) 416
Mph1103I ATGCAT 3 cut(s) 553, 733, 989
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 416
MseI TTAA 8 cut(s) 80, 101, 224, 900, 969, 1146, 1154, 1241
Msp20I TGGCCA 1 cut(s) 416
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 1 cut(s) 7
MspR9I CCNGG 1 cut(s) 611
MunI CAATTG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 611
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 3 cut(s) 552, 662, 795
NdeI CATATG 1 cut(s) 411
NdeII GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
NlaIII CATG 6 cut(s) 555, 622, 987, 1137, 1220, 1387
NsiI ATGCAT 3 cut(s) 553, 733, 989
NspI RCATGY 3 cut(s) 555, 987, 1220
PaeI GCATGC 2 cut(s) 555, 987
PaeR7I CTCGAG 2 cut(s) 1126, 1377
PasI CCCWGGG 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 8 cut(s) 125, 323, 349, 634, 716, 857, 881, 1292
PkrI GCNGC 2 cut(s) 604, 658
PleI GAGTC 2 cut(s) 123, 1369
PpsI GAGTC 2 cut(s) 123, 1369
PsiI TTATAA 2 cut(s) 455, 1005
Psp124BI GAGCTC 1 cut(s) 827
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspPI GGNCC 1 cut(s) 328
PspXI VCTCGAGB 2 cut(s) 1126, 1377
PsuI RGATCY 3 cut(s) 518, 571, 1312
RsaI GTAC 2 cut(s) 108, 617
RsaNI GTAC 2 cut(s) 107, 616
SacI GAGCTC 1 cut(s) 827
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 8 cut(s) 80, 101, 224, 900, 969, 1146, 1154, 1241
SatI GCNGC 2 cut(s) 603, 657
Sau3AI GATC 6 cut(s) 178, 518, 535, 571, 1312, 1386
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 2 cut(s) 124, 1369
ScrFI CCNGG 1 cut(s) 611
SduI GDGCHC 1 cut(s) 827
SfaNI GCATC 3 cut(s) 718, 740, 1384
Sfr274I CTCGAG 2 cut(s) 1126, 1377
SlaI CTCGAG 2 cut(s) 1126, 1377
SmlI CTYRAG 4 cut(s) 826, 968, 1126, 1377
SmoI CTYRAG 4 cut(s) 826, 968, 1126, 1377
SphI GCATGC 2 cut(s) 555, 987
SsiI CCGC 3 cut(s) 686, 798, 872
SspMI CTAG 2 cut(s) 15, 569
SstI GAGCTC 1 cut(s) 827
StyD4I CCNGG 1 cut(s) 609
StyI CCWWGG 1 cut(s) 1086
TaaI ACNGT 3 cut(s) 583, 926, 1353
TaiI ACGT 1 cut(s) 487
TaqI TCGA 3 cut(s) 1127, 1378, 1392
TfiI GAWTC 8 cut(s) 125, 323, 349, 634, 716, 857, 881, 1292
Tru1I TTAA 8 cut(s) 80, 101, 224, 900, 969, 1146, 1154, 1241
Tru9I TTAA 8 cut(s) 80, 101, 224, 900, 969, 1146, 1154, 1241
TscAI CASTG 4 cut(s) 245, 586, 931, 1273
TseI GCWGC 2 cut(s) 602, 656
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 4 cut(s) 245, 586, 931, 1273
Vha464I CTTAAG 1 cut(s) 968
XapI RAATTY 4 cut(s) 34, 540, 805, 1177
XceI RCATGY 3 cut(s) 555, 987, 1220
XhoI CTCGAG 2 cut(s) 1126, 1377
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 15, 569
Zsp2I ATGCAT 3 cut(s) 553, 733, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.