pycom15g34850

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012629
Physical Location & Seq
Forward (+)
34660254 .. 34667566
7313 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4992 bp
ATGACAACCCTAAGATCAAACTTGCCGTCGAGGATTCGGCAACTTCTTTCAGCCGACGGTGCTCTCGGGCCATCCATCAAACTCGATTCCGAGCCACCTCCTAAGATCAAAGCCTTTATCAATAAAGTGATACAATGTCCATTACAAGATATAGCAATACCTCTTTCCGGTTTCCGTTGGGAGTACATTAAGGGTAATTTTCACCATTGGAGGCCGCTTTTTCTGCATTTTGATACATATTTCAAGACATACTTGTCTTGTAGAAATGATCTACTGTTGTCGGATAAAATCTTAGAAGATGATAGTCCGTTTCCAAAGCATGCTGTGCTACAAATTTTGAGAGTGATGCAAACAATTTTAGAAAACTGCAATAACAAGAGTTCATTTGATGGCTTAGAGCATTTCAAGCTCCTGCTTGCATCAACAGATCCCGAGGTCCTTATAGCTGCATTAGAAACTCTTTCCGCACTTGTGAAAATAAATCCCTCCAAGATGCACGTCAGTGGGAAGATGATCGGTTGTGGTTCTGTAAACAGCTATCTCCTGTCTCTGGCACAGGGCTGGGGAAGCAAGGAAGAGGGATTGGGTTTATATTCATGTGTTATGGCAAACGAGACTACCCAAGATGACGGGCTCAATTTGTTCCCATCTGATGTAGAAAATGATTCTCACAAGTTGCAGTGCCGAATAGGTTCTACGCTCTATTTTGAATTGCATGGGGATGCCCAAAGTACAGAGGAGAGCTCTAACAACGTAAATAATTCTTCCAGTTTTGGGGTTATAAACATTCCAGATTTGCATTTGCAAAAGGAGGATGATTTAAAGCTAATGAAACGATATATAGAGGAGTACAGGGTACCTCCTGAACTCCGGTTCTCACTACTGACAAGAGTCAGATATGCTCGTGCCTTCCGTTCTCCGAAAATATGTAGGCTGTACAGCAGGATTTGCCTTCTTGCATTTGTTGTGCTTGTTCAGTCTAGTGATGCCAGTGTAGAACTGAATTCCTTCTTTGCTAATGAGCCAGAGTACACAAATGAATTAATCAGAATTGTGCGGTCCGAAGAATCGGTGTCTGGGACCATCAGAACACAGGCAATGCTTGCGTTGGGTGCTCAGTTAGCTGCATACTCAGCGTCTCATGAGCGGGCACGAATCTTGAGTGTATCTAGTATCAGTTTTGCAGGGGGAAATCGTATGATACTCCTGAATGTGCTACAGAGGGCTGTTAGATCATTAAAAAACTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCACTTCTTCAGTTCTATCTGCTCCATGTGGTTTCATCATCAGCTACAGGGAGCAATATTAGAGGGTCAGGCATGGTTCCGACATTCTTGCCACTTTTGGAAGATTCTGATCCTTTGCATTTGCATCTAGTCTGTTTTGCTGTCAAAACTCTACAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTGTTTAAAGAGTCAGGGGGAGTTGAGCTTTTGTCTCAGAGACTACAGATTGAGGTACAGAGAGTTATTGGTTTGGCTGGCGATAATGATAACTCAATGGTTATTGGTGAAAGCTCAAGATACAGTGACAACCAGCTGTACTCACAGAAGAGGCTCATCAAAGCTTCATTGAAGGCACTTGGGTCTGCTACATATGGCGCTGGGAATTCCTCCAGAGTGCAACACTCCCATGACAGTTCTTTACCTGCAACTCTTTCTCTGATATTTAGGAATGTGGACAAATTTGGCGGGGACATTTATTACTCCGCAGTGACTGTTTTGAGTGAAACTATCCACAAAGACCCAACTTGCTTTTCTGCTTTGCATGAAATGGGTCTCCCAGATGCATTTATATCCTCTGTTGTAGCTGGCATTCTTCCATCCGCAAAGGCTTTTACTTGTGTACCTAACGGTCTTGGCGCTATTTGTCTTAATGCCAAAGGCTTGGAGGCTGTGAAGGAAAGTTTAGCGTTACGCTTTCTTGTTGACATCTTCACGAGCAAGAAATATGTTACGGCAATGAATGAAGCCATCGTTCCTTTGGCAAATGCAGTGGAAGAGCTTTTCCGCCATGTATCTTCGCTAAGAAGCACTGGTGTCGATATCATCCTTGAGATAATTGATAAAATTGCTTCCTTCACTGACAGCCACAATACAGGGCCAGAAGGAAAGGCAAATGGGAGTACTGCTATGGAAATGGATTCTGAAGATAGAGAACATGAAGGTCATTGCTGTCTGGTTGATTCTGTAGATTCGGCTGCAGATGGGATCAGTAATGAGCAGTTCATTCAATTATCCATCTTTCATTTGATGATACTGGTCCACAGAACAATGGAGAATTCCGAAACATGTCGGTTGTTTGTCGAGAAGTCAGGAATTGATGCATTACTGAAGCTTCTTCTCCAACCCACTATTGTACAATCATCTGATGGGATGTCTATTGCTTTGCACAGTACTATGGTTTTCAAGGGTTTTACTCAACATCACTCTGCTGCCCTTGCCCGTGCATTTTGCTCCTCCCTTAGGGACCACCTAAAGAAAGCTTTATCTGGATTTGGGGCAGTTTCAGGATCCTTTCTACTGGAACCTAGGATGGCATCAGATGGTGGAATTTTTTCTTCTCTGTTTCTTGTTGAGTTCCTCTTGTTTATTGCTGCCTCAAAGGACAACCGCTGGGTAACTGCATTGCTTACGGAATTTGGAAATGGTAGCAAGGATGTTCTTGAAGATATAGGATGTGTCCATCGTGAAGTTTCCTGGCAGATTGCTCTTCTAGAAGATACAAAGTCTGACGTGGAGGATGATAATGCTGGTTCTACCACTGAGTCACAACAGTCGGAAACAAATACAAGTGAAACTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTCCTTGACCCGTTGCTCACAAGGAGGACTTCTGGCTGGAGCATTGAATCCCAGTTTCTGGACCTTATTAGCCTATACCGTGACCTTGGTCGCTCCACTAGTTCTCAACAAAGGACAAATTCTGATGGTCCTTCAAATCTTCGGATTGGATCCAGTCATCAATTCCAACATTCTGGGTCTTCAGATGCTGTTGGTCCTCTTAATAAAAAGGAGTATGACCAGCAGAGGTCATATTATACTTCTTGCTGTGACATGGTGAGATCACTTTCTTTTCACATTACCCATCTGTTTCAAGAGATGGGGAAGGTCATGTCGGTACCTTCTCGTCGACGGGATGATATAGTGAATGTTTCTCCTGCTGCAAAATCTGTGGCGTCTACTTTTGCGTCCATTGCATTTGATCACTTGAACTTCGAAGGGCCTGCTAATTCTTCTGGATCCAAGGATTCCATATCAACAAAATGTCGGTACTTTGGTAAAGTTATTGATTTCATTGATGTAAGTCTACTGGAGAGAGCAGATTCATGTAATGCTGTTTTACTAAATTGCTTATATGGTCATGGAGTTATCCAGTCAGTTTTGAAGACATTTGTAGCTACCAGTGAATTGCTCTTCACAGTTAGGGCTCCTGCATCCCCCATGGAAACAGATGATGGGAATTCAAAGCAGGATGAAAGGGAAGATATTGACCATTCATGGATTTATGGTCCTTTAGCTAGCTATGGTAAACTTATGGACCACCTAGTGACATCTTCTTTCATTTTATCTCCTTTCACAAAACACTTGCTAGCACAACCCCTGGCAAATGGAAATATTCCTTTCCCGCGCGATGCTGAGACCTTTGTAAAGGTCCTCCAGTCCATGGTACTGAAAGCAATTCTTCCTCTTTGGACTCATCCTCAGTTTGTTGATTGTAGTTATGATTTTATTAGTACCGTTATTTCTATAATCAGGCATGTATACTCTGGGGTTGAAGTGAAGAATGTTAGTAGCAGCAACACTGCCCGTATTACTGGCCCCCCTCCTAATGAGACAACTATTTCAATGATTGTGGAGATGGGCTTTACCAGGTCTAGGGCAGAAGAAGCTCTGAGGCAAGTTGGATCAAATAGTGTGGAGTTGGCAATGGAGTGGTTGTTTTCCCATCAAGAAGAGACTGAAGAAGACAATGAACTTGCTCGGGCACTTGCCATGTCTTTGGGTAATGAATCAGACACTAAGGAAGCTGTTGCAAATGAGAATGCTCAGCAGCTTGAAGAAGAGATAGTCCAACTTCCTCCTGTTGAGGAGTTGATATCTACATGTGCAAAGCTTCTGCAGATGAAGGAGCCTCTAGCTTTTCCAGTTCGAGACCTGCTAGTAATGATATGCTCCCAAAATGATGGTCAATATCGGCCTAATATCATCTCTTTCATTGTTGATCAGATTAAAGAATGTAGTTTATGTTTTGACAGTGGAAAAAGTACTTTGCTATCTGCTCTTTTTCACGTTCTTGCTTTAATTCTTCAAGAAGACGCAATTGCACGAGAACTTGCCTCGAAGAATGGTCTTGTGAAAGTTGCATCAGACCTACTCTTCCAGTGGGATTCTGGTTCAGTTGGCAGGGAGAAACATGAAGTTCCAAAGTGGGCAACAACGGCTTTTCTTGCTGTTGATCGGCTGTTGCAGGTGGATCAAAAACTGAACCCTGAAATTGCAGAACAGTTGAAGAAGGATGGTATCAGTAGCCACCAAACACCACTTAGCATTGATGAGGACAAGCAAAATGAGTTGCAGTCTGCATTGGGATTATCTTCAAAGCATATAGAAATGAAAGAGCAGAAGAGGCTTATTGAGATAGCTTGTTCTTGCATCAGGAACCAGCTTCCTTCTGAAACAATGCATGCTGTTCTGCAGCTTTGCTCTACTCTCACAAAAACTCATGCTGTTGCTGTTCAATTTCTTGATGCCGGGGGTTTAAGTTTACTGCTTTCTTTGCCAACAAGTTGCCTCTTTCCTGGGTTTGACAATATTGCTGCTACCATTATCCGTCATGTTCTGGAACGATCGATCGACGGCGACCGGAACTCAAACTCTTCAGCAGGCAATGGAATTTGA

Protein Analysis

1664

Amino Acids

183.35

Weight (kDa)

5.52

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 107 - 168 1.5e-11 Domain of Unknown Function (DUF908)
DUF908 PF06012 242 - 410 8.1e-21 Domain of Unknown Function (DUF908)
DUF913 PF06025 473 - 805 3.5e-63 Domain of Unknown Function (DUF913)
UBA PF00627 1307 - 1344 3.1e-09 UBA/TS-N domain
UBA_7 PF22562 1308 - 1354 1.2e-14 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AarI CACCTGC 1 cut(s) 4552
AasI GACNNNNNNGTC 1 cut(s) 253
Acc36I ACCTGC 3 cut(s) 1720, 4257, 4552
Acc65I GGTACC 2 cut(s) 856, 3211
AccB1I GGYRCC 2 cut(s) 856, 3211
AccB7I CCANNNNNTGG 2 cut(s) 2953, 3757
AccBSI CCGCTC 1 cut(s) 1147
AccI GTMKAC 4 cut(s) 3223, 3272, 3400, 3855
AccII CGCG 2 cut(s) 3721, 3723
AcuI CTGAAG 7 cut(s) 1271, 2232, 2417, 2886, 3060, 4074, 4956
AcyI GRCGYC 1 cut(s) 3269
AdeI CACNNNGTG 1 cut(s) 3640
AfiI CCNNNNNNNGG 7 cut(s) 167, 550, 774, 2529, 2953, 3757, 3969
AflIII ACRYGT 2 cut(s) 2354, 4196
AhlI ACTAGT 1 cut(s) 2993
AjiI CACGTC 2 cut(s) 499, 2800
AjnI CCWGG 4 cut(s) 2762, 3693, 3962, 4891
AjuI GAANNNNNNNTTGG 2 cut(s) 1804, 1836
AleI CACNNNNGTG 1 cut(s) 501
Alw21I GWGCWC 3 cut(s) 64, 746, 1117
AlwNI CAGNNNCTG 3 cut(s) 1781, 2953, 3083
Ama87I CYCGRG 3 cut(s) 65, 431, 4074
AoxI GGCC 6 cut(s) 68, 212, 2165, 3314, 3910, 4289
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 4264, 4296
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 4 cut(s) 691, 1007, 1281, 2620
Asp718I GGTACC 2 cut(s) 856, 3211
AspA2I CCTAGG 1 cut(s) 2594
AspLEI GCGC 3 cut(s) 1667, 1928, 3723
AsuC2I CCSGG 1 cut(s) 4846
AsuHPI GGTGA 3 cut(s) 194, 1586, 3163
AsuII TTCGAA 1 cut(s) 3309
AsuNHI GCTAGC 2 cut(s) 3611, 3682
AvaI CYCGRG 3 cut(s) 65, 431, 4074
AvrII CCTAGG 1 cut(s) 2594
AxyI CCTNAGG 1 cut(s) 2528
BaeGI GKGCMC 2 cut(s) 1153, 4081
BaeI ACNNNNGTAYC 4 cut(s) 1893, 1926, 3355, 3388
BamHI GGATCC 3 cut(s) 2576, 3044, 3332
BanI GGYRCC 2 cut(s) 856, 3211
BanII GRGCYC 3 cut(s) 636, 746, 3523
BauI CACGAG 3 cut(s) 903, 2002, 4419
BbsI GAAGAC 4 cut(s) 3066, 3485, 4065, 4413
Bbv12I GWGCWC 3 cut(s) 64, 746, 1117
BceAI ACGGC 4 cut(s) 10, 2037, 4548, 4966
BcgI CGANNNNNNTGC 6 cut(s) 1348, 1382, 2086, 2120, 3299, 3333
BciT130I CCWGG 4 cut(s) 2764, 3695, 3964, 4893
BclI TGATCA 2 cut(s) 3295, 4315
BcnI CCSGG 1 cut(s) 4846
BcuI ACTAGT 1 cut(s) 2993
BfmI CTRYAG 7 cut(s) 1217, 1323, 1511, 2253, 2265, 4211, 4787
BfoI RGCGCY 2 cut(s) 1668, 1929
BfuAI ACCTGC 3 cut(s) 1720, 4257, 4552
BlnI CCTAGG 1 cut(s) 2594
BlpI GCTNAGC 1 cut(s) 4140
BmcAI AGTACT 3 cut(s) 2191, 2461, 4360
Bme1390I CCNGG 5 cut(s) 2764, 3695, 3964, 4846, 4893
BmeT110I CYCGRG 3 cut(s) 65, 431, 4074
BmgBI CACGTC 2 cut(s) 499, 2800
BmrFI CCNGG 5 cut(s) 2764, 3695, 3964, 4846, 4893
BmrI ACTGGG 1 cut(s) 2941
BmtI GCTAGC 2 cut(s) 3615, 3686
BmuI ACTGGG 1 cut(s) 2941
BoxI GACNNNNGTC 2 cut(s) 890, 2982
BpiI GAAGAC 4 cut(s) 3066, 3485, 4065, 4413
BplI GAGNNNNNCTC 2 cut(s) 728, 760
BpmI CTGGAG 4 cut(s) 1663, 2953, 3425, 3734
Bpu1102I GCTNAGC 1 cut(s) 4140
Bpu14I TTCGAA 1 cut(s) 3309
BpuEI CTTGAG 3 cut(s) 1180, 1567, 2138
BpuMI CCSGG 1 cut(s) 4846
Bsa29I ATCGAT 1 cut(s) 4943
BsaBI GATNNNNATC 1 cut(s) 1386
BsaHI GRCGYC 1 cut(s) 3269
BsaI GGTCTC 3 cut(s) 1847, 3725, 4239
BsaJI CCNNGG 9 cut(s) 432, 2594, 2980, 3336, 3534, 3693, 3756, 4845, 4892
BsaWI WCCGGW 3 cut(s) 167, 870, 4956
BsaXI ACNNNNNCTCC 2 cut(s) 4016, 4046
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 550, 774, 2529, 2953, 3757, 3969
Bse21I CCTNAGG 1 cut(s) 2528
Bse3DI GCAATG 7 cut(s) 1104, 2031, 2233, 2690, 3285, 4026, 4987
Bse8I GATNNNNATC 1 cut(s) 1386
BseBI CCWGG 4 cut(s) 2764, 3695, 3964, 4893
BseCI ATCGAT 1 cut(s) 4943
BseDI CCNNGG 9 cut(s) 432, 2594, 2980, 3336, 3534, 3693, 3756, 4845, 4892
BseJI GATNNNNATC 1 cut(s) 1386
BseLI CCNNNNNNNGG 7 cut(s) 167, 550, 774, 2529, 2953, 3757, 3969
BseMI GCAATG 7 cut(s) 1104, 2031, 2233, 2690, 3285, 4026, 4987
BseMII CTCAG 8 cut(s) 1130, 1146, 1517, 2820, 3720, 3809, 3977, 4154
BseRI GAGGAG 4 cut(s) 752, 860, 2512, 4196
BseSI GKGCMC 2 cut(s) 1153, 4081
BseYI CCCAGC 3 cut(s) 561, 1667, 2679
Bsh1236I CGCG 2 cut(s) 3721, 3723
Bsh1285I CGRYCG 3 cut(s) 4943, 4947, 4957
BshFI GGCC 6 cut(s) 70, 214, 2167, 3316, 3912, 4291
BshNI GGYRCC 2 cut(s) 856, 3211
BshVI ATCGAT 1 cut(s) 4943
BsiEI CGRYCG 3 cut(s) 4943, 4947, 4957
BsiHKAI GWGCWC 3 cut(s) 64, 746, 1117
BsiHKCI CYCGRG 3 cut(s) 65, 431, 4074
BsiSI CCGG 4 cut(s) 168, 871, 4845, 4957
BslFI GGGAC 3 cut(s) 1093, 1772, 2546
BslI CCNNNNNNNGG 7 cut(s) 167, 550, 774, 2529, 2953, 3757, 3969
BsmBI CGTCTC 1 cut(s) 1143
BsmFI GGGAC 3 cut(s) 1093, 1772, 2546
BsmI GAATGC 2 cut(s) 1878, 4141
BsnI GGCC 6 cut(s) 70, 214, 2167, 3316, 3912, 4291
Bso31I GGTCTC 3 cut(s) 1847, 3725, 4239
BsoBI CYCGRG 3 cut(s) 65, 431, 4074
Bsp119I TTCGAA 1 cut(s) 3309
Bsp1286I GDGCHC 7 cut(s) 64, 636, 746, 1117, 1153, 3523, 4081
Bsp1407I TGTACA 2 cut(s) 936, 2422
Bsp1720I GCTNAGC 1 cut(s) 4140
Bsp19I CCATGG 2 cut(s) 3534, 3756
BspANI GGCC 6 cut(s) 70, 214, 2167, 3316, 3912, 4291
BspCNI CTCAG 8 cut(s) 1129, 1145, 1516, 2821, 3721, 3808, 3978, 4153
BspDI ATCGAT 1 cut(s) 4943
BspFNI CGCG 2 cut(s) 3721, 3723
BspHI TCATGA 1 cut(s) 1141
BspMAI CTGCAG 3 cut(s) 2269, 4215, 4791
BspMI ACCTGC 3 cut(s) 1720, 4257, 4552
BspOI GCTAGC 2 cut(s) 3615, 3686
BspQI GCTCTTC 4 cut(s) 2058, 2781, 2862, 3512
BspT104I TTCGAA 1 cut(s) 3309
BspT107I GGYRCC 2 cut(s) 856, 3211
BspTNI GGTCTC 3 cut(s) 1847, 3725, 4239
BsrBI CCGCTC 1 cut(s) 1147
BsrDI GCAATG 7 cut(s) 1104, 2031, 2233, 2690, 3285, 4026, 4987
BsrGI TGTACA 2 cut(s) 936, 2422
BssECI CCNNGG 9 cut(s) 432, 2594, 2980, 3336, 3534, 3693, 3756, 4845, 4892
BssNAI GTATAC 1 cut(s) 3856
BssNI GRCGYC 1 cut(s) 3269
BssSI CACGAG 3 cut(s) 903, 2002, 4419
BssT1I CCWWGG 5 cut(s) 2594, 2980, 3336, 3534, 3756
Bst1107I GTATAC 1 cut(s) 3856
Bst2BI CACGAG 3 cut(s) 903, 2002, 4419
Bst2UI CCWGG 4 cut(s) 2764, 3695, 3964, 4893
BstACI GRCGYC 1 cut(s) 3269
BstAPI GCANNNNNTGC 3 cut(s) 325, 948, 1103
BstAUI TGTACA 2 cut(s) 936, 2422
BstBI TTCGAA 1 cut(s) 3309
BstDSI CCRYGG 2 cut(s) 3534, 3756
BstENI CCTNNNNNAGG 1 cut(s) 2527
BstFNI CGCG 2 cut(s) 3721, 3723
BstH2I RGCGCY 2 cut(s) 1668, 1929
BstHHI GCGC 3 cut(s) 1667, 1928, 3723
BstMCI CGRYCG 3 cut(s) 4943, 4947, 4957
BstNI CCWGG 4 cut(s) 2764, 3695, 3964, 4893
BstNSI RCATGY 5 cut(s) 323, 2358, 3854, 4200, 4781
BstPAI GACNNNNGTC 2 cut(s) 890, 2982
BstSCI CCNGG 5 cut(s) 2762, 3693, 3962, 4844, 4891
BstSFI CTRYAG 7 cut(s) 1217, 1323, 1511, 2253, 2265, 4211, 4787
BstSLI GKGCMC 2 cut(s) 1153, 4081
BstUI CGCG 2 cut(s) 3721, 3723
BstV2I GAAGAC 4 cut(s) 3066, 3485, 4065, 4413
BstX2I RGATCY 4 cut(s) 427, 2576, 3044, 3332
BstXI CCANNNNNNTGG 4 cut(s) 1951, 3068, 4093, 4277
BstYI RGATCY 4 cut(s) 427, 2576, 3044, 3332
BstZ17I GTATAC 1 cut(s) 3856
Bsu15I ATCGAT 1 cut(s) 4943
Bsu36I CCTNAGG 1 cut(s) 2528
BsuRI GGCC 6 cut(s) 70, 214, 2167, 3316, 3912, 4291
BsuTUI ATCGAT 1 cut(s) 4943
BtgI CCRYGG 2 cut(s) 3534, 3756
BtgZI GCGATG 1 cut(s) 3738
BtrI CACGTC 2 cut(s) 499, 2800
BtsI GCAGTG 4 cut(s) 686, 1782, 2064, 3894
BveI ACCTGC 3 cut(s) 1720, 4257, 4552
CaiI CAGNNNCTG 3 cut(s) 1781, 2953, 3083
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 3 cut(s) 1667, 1928, 3723
ClaI ATCGAT 1 cut(s) 4943
CpoI CGGWCCG 1 cut(s) 1059
CseI GACGC 4 cut(s) 1125, 3258, 3270, 4418
CsiI ACCWGGT 1 cut(s) 3962
CspCI CAANNNNNGTGG 8 cut(s) 2040, 2075, 3246, 3281, 3927, 3962, 3990, 4025
CspI CGGWCCG 1 cut(s) 1059
DraI TTTAAA 2 cut(s) 822, 1474
DraIII CACNNNGTG 1 cut(s) 3640
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 2063
Ecl136II GAGCTC 1 cut(s) 744
Eco130I CCWWGG 5 cut(s) 2594, 2980, 3336, 3534, 3756
Eco24I GRGCYC 3 cut(s) 636, 746, 3523
Eco31I GGTCTC 3 cut(s) 1847, 3725, 4239
Eco32I GATATC 2 cut(s) 2110, 4191
Eco53kI GAGCTC 1 cut(s) 744
Eco57I CTGAAG 7 cut(s) 1271, 2232, 2417, 2886, 3060, 4074, 4956
Eco81I CCTNAGG 1 cut(s) 2528
Eco88I CYCGRG 3 cut(s) 65, 431, 4074
EcoICRI GAGCTC 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 2527
EcoO109I RGGNCCY 3 cut(s) 436, 3314, 3745
EcoRI GAATTC 4 cut(s) 1003, 1672, 2344, 3553
EcoRII CCWGG 4 cut(s) 2762, 3693, 3962, 4891
EcoRV GATATC 2 cut(s) 2110, 4191
EcoT14I CCWWGG 5 cut(s) 2594, 2980, 3336, 3534, 3756
EcoT22I ATGCAT 3 cut(s) 1855, 2392, 4779
EcoT38I GRGCYC 3 cut(s) 636, 746, 3523
ErhI CCWWGG 5 cut(s) 2594, 2980, 3336, 3534, 3756
Esp3I CGTCTC 1 cut(s) 1143
FalI AAGNNNNNCTT 8 cut(s) 236, 268, 1269, 1301, 2908, 2940, 3759, 3791
FaqI GGGAC 3 cut(s) 1093, 1772, 2546
FauI CCCGC 3 cut(s) 1140, 1748, 3726
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 2 cut(s) 3295, 4315
FblI GTMKAC 4 cut(s) 3223, 3272, 3400, 3855
FriOI GRGCYC 3 cut(s) 636, 746, 3523
GlaI GCGC 3 cut(s) 1666, 1927, 3722
GsaI CCCAGC 3 cut(s) 565, 1671, 2683
GsuI CTGGAG 4 cut(s) 1663, 2953, 3425, 3734
HaeII RGCGCY 2 cut(s) 1668, 1929
HaeIII GGCC 6 cut(s) 70, 214, 2167, 3316, 3912, 4291
HapII CCGG 4 cut(s) 168, 871, 4845, 4957
HgaI GACGC 4 cut(s) 1125, 3258, 3270, 4418
HhaI GCGC 3 cut(s) 1667, 1928, 3723
Hin1I GRCGYC 1 cut(s) 3269
Hin6I GCGC 3 cut(s) 1665, 1926, 3721
HinP1I GCGC 3 cut(s) 1665, 1926, 3721
HincII GTYRAC 2 cut(s) 1993, 3224
HindII GTYRAC 2 cut(s) 1993, 3224
HindIII AAGCTT 4 cut(s) 1629, 2399, 2547, 4205
HpaII CCGG 4 cut(s) 168, 871, 4845, 4957
HphI GGTGA 3 cut(s) 194, 1586, 3163
Hpy99I CGWCG 5 cut(s) 31, 59, 3225, 3228, 4952
HpyCH4IV ACGT 4 cut(s) 498, 753, 2799, 4383
HpySE526I ACGT 4 cut(s) 498, 753, 2799, 4383
Hsp92I GRCGYC 1 cut(s) 3269
HspAI GCGC 3 cut(s) 1665, 1926, 3721
KpnI GGTACC 2 cut(s) 860, 3215
Ksp22I TGATCA 2 cut(s) 3295, 4315
LguI GCTCTTC 4 cut(s) 2058, 2781, 2862, 3512
LmnI GCTCC 9 cut(s) 414, 1305, 1329, 2525, 2934, 2993, 3526, 4222, 4271
MabI ACCWGGT 1 cut(s) 3962
MaeII ACGT 4 cut(s) 498, 753, 2799, 4383
MaeIII GTNAC 9 cut(s) 1592, 1777, 1977, 2017, 2683, 2832, 2975, 3143, 3640
MbiI CCGCTC 1 cut(s) 1147
MfeI CAATTG 1 cut(s) 4413
MflI RGATCY 4 cut(s) 427, 2576, 3044, 3332
MhlI GDGCHC 7 cut(s) 64, 636, 746, 1117, 1153, 3523, 4081
MlyI GAGTC 4 cut(s) 900, 1487, 2840, 3781
MmeI TCCRAC 8 cut(s) 261, 1280, 1382, 2434, 2823, 3085, 3976, 4189
Mph1103I ATGCAT 3 cut(s) 1855, 2392, 4779
MroXI GAANNNNTTC 4 cut(s) 691, 1007, 1281, 2620
MslI CAYNNNNRTG 3 cut(s) 501, 720, 1695
MspA1I CMGCKG 3 cut(s) 1460, 1603, 2679
MspI CCGG 4 cut(s) 168, 871, 4845, 4957
MspR9I CCNGG 5 cut(s) 2764, 3695, 3964, 4846, 4893
MunI CAATTG 1 cut(s) 4413
Mva1269I GAATGC 2 cut(s) 1878, 4141
MvaI CCWGG 4 cut(s) 2764, 3695, 3964, 4893
MvnI CGCG 2 cut(s) 3721, 3723
NciI CCSGG 1 cut(s) 4846
NcoI CCATGG 2 cut(s) 3534, 3756
NdeI CATATG 1 cut(s) 1660
NheI GCTAGC 2 cut(s) 3611, 3682
NmuCI GTSAC 6 cut(s) 1592, 1777, 2832, 2975, 3143, 3640
NsiI ATGCAT 3 cut(s) 1855, 2392, 4779
NspI RCATGY 5 cut(s) 323, 2358, 3854, 4200, 4781
NspV TTCGAA 1 cut(s) 3309
OliI CACNNNNGTG 1 cut(s) 501
PaeI GCATGC 2 cut(s) 323, 4781
PagI TCATGA 1 cut(s) 1141
PaqCI CACCTGC 1 cut(s) 4552
PciI ACATGT 2 cut(s) 2354, 4196
PciSI GCTCTTC 4 cut(s) 2058, 2781, 2862, 3512
PcsI WCGNNNNNNNCGW 1 cut(s) 910
PctI GAATGC 2 cut(s) 1878, 4141
PdmI GAANNNNTTC 4 cut(s) 691, 1007, 1281, 2620
PflMI CCANNNNNTGG 2 cut(s) 2953, 3757
Ple19I CGATCG 2 cut(s) 4943, 4947
PleI GAGTC 4 cut(s) 899, 1486, 2839, 3781
PpsI GAGTC 4 cut(s) 899, 1486, 2839, 3781
PpuMI RGGWCCY 2 cut(s) 436, 3745
PscI ACATGT 2 cut(s) 2354, 4196
PshAI GACNNNNGTC 2 cut(s) 890, 2982
PshBI ATTAAT 1 cut(s) 1043
PsiI TTATAA 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 746
Psp5II RGGWCCY 2 cut(s) 436, 3745
Psp6I CCWGG 4 cut(s) 2762, 3693, 3962, 4891
PspFI CCCAGC 3 cut(s) 561, 1667, 2679
PspGI CCWGG 4 cut(s) 2762, 3693, 3962, 4891
PspPPI RGGWCCY 2 cut(s) 436, 3745
PstI CTGCAG 3 cut(s) 2269, 4215, 4791
PstNI CAGNNNCTG 3 cut(s) 1781, 2953, 3083
PsuI RGATCY 4 cut(s) 427, 2576, 3044, 3332
PvuI CGATCG 2 cut(s) 4943, 4947
PvuII CAGCTG 2 cut(s) 1460, 1603
RseI CAYNNNNRTG 3 cut(s) 501, 720, 1695
Rsr2I CGGWCCG 1 cut(s) 1059
RsrII CGGWCCG 1 cut(s) 1059
SacI GAGCTC 1 cut(s) 746
SalI GTCGAC 1 cut(s) 3222
SapI GCTCTTC 4 cut(s) 2058, 2781, 2862, 3512
ScaI AGTACT 3 cut(s) 2191, 2461, 4360
SchI GAGTC 4 cut(s) 900, 1487, 2840, 3781
ScrFI CCNGG 5 cut(s) 2764, 3695, 3964, 4846, 4893
SduI GDGCHC 7 cut(s) 64, 636, 746, 1117, 1153, 3523, 4081
SexAI ACCWGGT 1 cut(s) 3962
SfcI CTRYAG 7 cut(s) 1217, 1323, 1511, 2253, 2265, 4211, 4787
SfuI TTCGAA 1 cut(s) 3309
SgrDI CGTCGACG 1 cut(s) 3222
SmiMI CAYNNNNRTG 3 cut(s) 501, 720, 1695
SmlI CTYRAG 3 cut(s) 1159, 1582, 2117
SmoI CTYRAG 3 cut(s) 1159, 1582, 2117
SpeI ACTAGT 1 cut(s) 2993
SphI GCATGC 2 cut(s) 323, 4781
SspI AATATT 3 cut(s) 1336, 3709, 4906
SstI GAGCTC 1 cut(s) 746
StyD4I CCNGG 5 cut(s) 2762, 3693, 3962, 4844, 4891
StyI CCWWGG 5 cut(s) 2594, 2980, 3336, 3534, 3756
TaiI ACGT 4 cut(s) 501, 756, 2802, 4386
TauI GCSGC 1 cut(s) 217
TseFI GTSAC 6 cut(s) 1592, 1777, 2832, 2975, 3143, 3640
Tsp45I GTSAC 6 cut(s) 1592, 1777, 2832, 2975, 3143, 3640
TspGWI ACGGA 5 cut(s) 164, 297, 902, 2714, 4913
Van91I CCANNNNNTGG 2 cut(s) 2953, 3757
VspI ATTAAT 1 cut(s) 1043
XagI CCTNNNNNAGG 1 cut(s) 2527
XbaI TCTAGA 1 cut(s) 2779
XceI RCATGY 5 cut(s) 323, 2358, 3854, 4200, 4781
XcmI CCANNNNNNNNNTGG 2 cut(s) 2044, 4481
XmaJI CCTAGG 1 cut(s) 2594
XmiI GTMKAC 4 cut(s) 3223, 3272, 3400, 3855
XmnI GAANNNNTTC 4 cut(s) 691, 1007, 1281, 2620
ZrmI AGTACT 3 cut(s) 2191, 2461, 4360
Zsp2I ATGCAT 3 cut(s) 1855, 2392, 4779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.