pycom15g36490

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008981
Physical Location & Seq
Forward (+)
36496148 .. 36496545
398 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 282 bp
ATGGATTCGGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCAGAACAGGATGCTCAACACATTCTGAACAACGCTAGAAACTCGAAGATGGCACGCTTGAGACAAGCTAAAGAAGAAGCTGATAGGGAAGTGCAACTCTATCGCACGAATATGGAGGCTGAGCACCAAAAGAAAATTTCAGATGTACTAACTGGTCTTTCATCAACTACCCAATTATATCATGATCACTCCATTGTTCCCCAAAAAAAAAAAATGATCCTCTTTGAAATGAGATAA

Protein Analysis

94

Amino Acids

10.74

Weight (kDa)

9.1

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 70 5e-19 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 1 cut(s) 180
AfaI GTAC 1 cut(s) 192
AgsI TTSAA 1 cut(s) 272
AluBI AGCT 2 cut(s) 113, 125
AluI AGCT 2 cut(s) 113, 125
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 37
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 81
BisI GCNGC 1 cut(s) 38
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 39
BmsI GCATC 3 cut(s) 24, 36, 46
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 202
BseGI GGATG 2 cut(s) 27, 61
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 2 cut(s) 229, 261
Bsp1720I GCTNAGC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 226
BspPI GGATC 1 cut(s) 256
BsrI ACTGG 1 cut(s) 202
BssMI GATC 2 cut(s) 229, 261
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 27, 61
BstKTI GATC 2 cut(s) 232, 264
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 2 cut(s) 229, 261
BstV1I GCAGC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 27, 61
Cac8I GCNNGC 1 cut(s) 100
CaiI CAGNNNCTG 1 cut(s) 37
CciI TCATGA 1 cut(s) 226
Csp6I GTAC 1 cut(s) 191
CviAII CATG 1 cut(s) 227
CviJI RGCY 3 cut(s) 113, 125, 164
CviKI_1 RGCY 3 cut(s) 113, 125, 164
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 1 cut(s) 165
DpnI GATC 2 cut(s) 231, 263
DpnII GATC 2 cut(s) 229, 261
FaeI CATG 1 cut(s) 230
FaiI YATR 3 cut(s) 158, 223, 228
FatI CATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 2 cut(s) 14, 68
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 81
GluI GCNGC 1 cut(s) 38
Hin1II CATG 1 cut(s) 230
HinfI GANTC 1 cut(s) 5
Hpy188I TCNGA 2 cut(s) 72, 187
Hpy188III TCNNGA 2 cut(s) 31, 227
HpyCH4V TGCA 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 229, 261
LpnPI CCDG 3 cut(s) 38, 44, 183
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 3 cut(s) 24, 36, 46
MaeI CTAG 1 cut(s) 81
MalI GATC 2 cut(s) 231, 263
MboI GATC 2 cut(s) 229, 261
MboII GAAGA 2 cut(s) 103, 131
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 2 cut(s) 180, 218
MnlI CCTC 4 cut(s) 8, 17, 154, 275
MslI CAYNNNNRTG 2 cut(s) 32, 155
NdeII GATC 2 cut(s) 229, 261
NlaIII CATG 1 cut(s) 230
PagI TCATGA 1 cut(s) 226
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 1 cut(s) 39
PstNI CAGNNNCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 32, 155
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 2 cut(s) 229, 261
SduI GDGCHC 1 cut(s) 171
SetI ASST 2 cut(s) 115, 127
SfaNI GCATC 3 cut(s) 24, 36, 46
SmiMI CAYNNNNRTG 2 cut(s) 32, 155
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
Sse9I AATT 2 cut(s) 180, 218
SspMI CTAG 1 cut(s) 81
TaqI TCGA 1 cut(s) 89
TasI AATT 2 cut(s) 180, 218
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 1 cut(s) 5
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 195
XapI RAATTY 1 cut(s) 180
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.