pycom16g00060

recombinational repair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003214
Physical Location & Seq
Forward (+)
30384 .. 36588
6205 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3087 bp
ATGTTTGGTCATGCGGATCATCTTCATGATTTCTTTGATTCAACTTGGAGACATTGCTCTTTTTTAAGTGGATGGATTGGCAGTTTAATCTCAAAATACTGCACGGACGTGATTGAGTTACCTGCAAGGAATTCACTTTCTAAGTTGGCTGAAGAAAGGGAAACTCAATTGTCAACCTTCATGGCTCAAAGGGAATTTATAGATATTAGTTCATCGGATAGTGAATTAGAGATAGAAGAAAGGGAATATGGCAACAGCAGAGTTCTTCCAAGCTCGCTGTCTGGACCTGGCTCAAATCCAAGGAGTAGAGATTATTCTGGGCAGTCTAGAAACGTGCCTTCTCCTAGAAGAGCATATGCGTCTAATGGCAACTCTCCTAATATTAATCCGCACACACAGGTGAAACAGAATTTTAATCCCAGTTCAAGTGATGATATAAGAACCTCAAATCGACATGCTGCTCGAGCACATAATGGTTATGCAGAACGGCCTCAAAGTAATCATTCACAAATTTATAATATTTCTGTTAAGGACTATGAAGCTCATAAGAGGACCCTTCCGCCGTCTATGCAGCTATCGGGACCAAGTACTTATGCAACTAATGGCAAGGGCCTTATGAGAGACTATTCAAGTAGGGGCTATGACAATGAATTTAATAGGTCTGAGAGCAGTGGGAGTAGGGGCCAGCCTCCCTCTTTCATGCATGGAAAGTCATTTTCCACTTCACAGTTTGCTAGTTCAAGTGACCCTGCATACCATGGTGGGACAGGTGACGATAGGGTGGCTGGCAGTGATGAGAGACTGATATATCAAGCAGCATTAGAGGATCTCAATCAACCAAAAGTTGAAGCTACTTTACCTGATGGTCTTCTGTCTGTCCCTCTTCTACGACATCAGAAAATTGCTCTGGCATGGATGCTTCAAAAGGAAACTAGAAGTCTGCATTGTCTAGGTGGAATCTTAGCAGATGATCAGGGCCTTGGTAAGACCATTTCAATGATTTCCCTTATACAAATGCAGAAGTATTTGGATTCAAAATCAAAATCTAGAGAGTTAGGCAATCAGAAAACTGAAGCTTTAAATTTGGATGATGATGAGGACAATCCCAGCGGTGGTTTGGATAAATTGAATAAAACTGAGCAACCTGATCATCTTCGATCAATTCCAGAAGTTAGTACATCTTCACGGTCAATCAGAAAGCAGAGGCCAGCAGCGGGTACATTGGTTGTGTGTCCAGCAAGTGTTCTTCGACAGTGGGCTAGGGAGCTGGATGAAAAGGTTGCAGAGGAAGCAAAGCTGCGTGTCCTTATTTATCATGGAGGCAGCCGGACGAAGAATCCTGAAGAACTGGCTAGCTATGACGTGATTCTCACAACATATGCTATTGTAACCAATGAAGTCCCAAAACAACCTTTAGTTGATGACGATGAACCTGATGAGAAAAATGGGGAAACTTATGGAGTACATTCCGATTTTTCAAGTGATAAGAAGAGAAAAAAGGCTTCGGTTGTTAATAAGAAGAGAAAGAGGGGCAAAAAAGGAATTGACAGTTCATCTATTGATTGCGGTTCTGGTGCACTTGCAAAAGTGGGTTGGTTTAGGGTGATACTAGATGAAGCTCAGACAATTAAGAATCACAGAACTCAAGTGGCTAGAGCGTGTTGTAGCCTTCGAGCAAAAAGAAGGTGGTGTTTGTCTGGTACTCCTATACAAAATACAATTGATGATTTATACAGTTACTTTAGATTTCTTAGATATGACCCCTATGCTGTATATAAGTCATTTTACAGTACCATTAAGGTTCCAATATCGAGAAATTCGATCCAAGGTTATAAGAAGCTCCAGGCAGTTCTGAGGGCTATAATGTTGCGCCGAACAAAAGGAACATTGATCGATGGGCAACCTATAATTGAGTTACCACCAAAAACAATAAACTTGAGTAAAGTGGAGTTCTCATCTGAGGAGCGTGCTTTCTATACCAAGCTAGAAGCTGATTCACGCACTAAGTTCAAGGCATATGCCGCTGCTGGCACAGTGAATCAAAACTATGCAAATATTCTTTTGATGCTTTTGCGCCTCCGGCAAGCTTGTGACCACCCTCAGCTTGTTAAAGCATATGACTCTGACTGTGTCGGGAAAGATTCTGTGCAAATGGCAAGGCGACTCCCAAAGGATATGATTTCCCATCTTTTGCATCTTCTGGAAACTTCATTGGCCATTTGTCGTGTATGCAAAGATCCACCTGAAGGCCCTGTTGTTACTATGTGTGGCCATGTATTCTGCTATCAGTGTGTTTCAGAACACTTGACGGGTGATGACAGTACATGCCCTGATGCTGGATGTAAAGAACAAGTTGGTTCTGATGTTGTTTTCTCCAAATCCACTCTCATAAGTTGCCTCTCCAATAATCTTGATGGTACTCCGATGAATTCTGAGTTGGGCGAGAAATCAATTGTGTTACAGAATGAGTATAGTTCATCTAAAGTCAGAGCTATTATTGAGATTTTGCAGTCACATTTGGAACATAACTCTACTGGAAGCAATGGAGACCCTGCTTTTGGAACCGAAATCACAGATAGTATATATTCTGGCTTTTCAAGCTCACCAAATAATGGACCAATCAAAACGATTATTTTCTCCCAGTGGACTGGCATGTTGGATTTAGTTGAGGCATCATTGAACGAGTATTGTATACAGTACAGAAGGCTTGATGGTACAATGAGTCTGGCATCAAGAGACAGGGCTGTGAAAGATTTTAACACTGATCCTGAGATCATTGTTATGCTCATGTCACTAAAGGCTGGAAACCTTGGTCTCAACATGGTTGCTGCATGTCATGTTATCCTTTTGGACCTGTGGTGGAATCCAACTACTGAAGATCAAGCTATTGATCGGGCACATAGAATTGGACAGACCCGACCTGTTACTGTAACACGTCTCACAATTAAGGATACAGTGGAGGACAGGATATTAGCTTTACAGGAAGAAAAGAGAAAAATGGTGGCATCTGCTTTTGGTGAAGATCATGGTGGGGGCTCTGCTACACGTCTGACTGTCGAGGATCTGAGGTACCTATTTATGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000785 GO:0000790 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006304 GO:0006305 GO:0006306 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007154 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009059 GO:0009292 GO:0009294 GO:0009605 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0009991 GO:0010468 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016458 GO:0016462 GO:0016567 GO:0016740 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0019787 GO:0022402 GO:0030162 GO:0031047 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031668 GO:0031974 GO:0031981 GO:0032259 GO:0032268 GO:0032269 GO:0032392 GO:0032434 GO:0032435 GO:0032446 GO:0032508 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042176 GO:0042177 GO:0042262 GO:0042623 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0044764 GO:0044786 GO:0045003 GO:0045005 GO:0045861 GO:0046483 GO:0046872 GO:0046914 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051276 GO:0051704 GO:0051716 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070035 GO:0070647 GO:0071103 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0140083 GO:0140096 GO:0140097 GO:1901360 GO:1901564 GO:1901576 GO:1901799 GO:1902298 GO:1902969 GO:1903047 GO:1903050 GO:1903051 GO:1903362 GO:1903363 GO:1990505 GO:2000058 GO:2000059
KEGG Pathways
Metabolic & Signaling
ko04918 map04918
Pfam Domains
Protein Families
Helicase_C SNF2_N zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

1029

Amino Acids

114.43

Weight (kDa)

7.55

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 298 - 702 9.3e-69 SNF2-related domain
zf-C3HC4 PF00097 741 - 777 8.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 741 - 777 1.2e-06 Zinc finger, C3HC4 type (RING finger)
Helicase_C PF00271 875 - 970 3.7e-19 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 516, 1833
Acc36I ACCTGC 1 cut(s) 130
Acc65I GGTACC 1 cut(s) 3069
AccB1I GGYRCC 1 cut(s) 3069
AccB7I CCANNNNNTGG 1 cut(s) 2612
AccI GTMKAC 1 cut(s) 2692
AciI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
AclWI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
AcoI YGGCCR 2 cut(s) 2214, 2269
AcsI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
AcuI CTGAAG 5 cut(s) 171, 1092, 1362, 2265, 2895
AfiI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
AflIII ACRYGT 2 cut(s) 2933, 3044
AjiI CACGTC 4 cut(s) 109, 1363, 2936, 3047
AjnI CCWGG 2 cut(s) 286, 1842
AjuI GAANNNNNNNTTGG 2 cut(s) 2420, 2452
AleI CACNNNNGTG 2 cut(s) 107, 398
Alw21I GWGCWC 2 cut(s) 469, 1579
Alw26I GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
Alw44I GTGCAC 1 cut(s) 1575
AlwI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 8 cut(s) 488, 610, 682, 976, 1205, 2214, 2248, 2269
ApaLI GTGCAC 1 cut(s) 1575
ApeKI GCWGC 8 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2828
ApoI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
ArsI GACNNNNNNTTYG 2 cut(s) 2540, 2572
AseI ATTAAT 1 cut(s) 384
Asp718I GGTACC 1 cut(s) 3069
AspLEI GCGC 2 cut(s) 1872, 2076
AspS9I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
AsuHPI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
AsuNHI GCTAGC 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
BaeGI GKGCMC 2 cut(s) 1579, 2899
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BalI TGGCCA 2 cut(s) 2216, 2271
BanI GGYRCC 1 cut(s) 3069
BanII GRGCYC 1 cut(s) 3038
BarI GAAGNNNNNNTAC 2 cut(s) 840, 872
BbsI GAAGAC 1 cut(s) 860
Bbv12I GWGCWC 2 cut(s) 469, 1579
BbvCI CCTCAGC 1 cut(s) 2100
BbvI GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BccI CCATC 6 cut(s) 66, 857, 1889, 2193, 2408, 2705
BceAI ACGGC 2 cut(s) 503, 547
BciT130I CCWGG 2 cut(s) 288, 1844
BciVI GTATCC 1 cut(s) 2944
BclI TGATCA 2 cut(s) 970, 1147
BcoDI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 2944
BisI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
BlsI GCNGC 9 cut(s) 460, 573, 817, 1213, 1299, 1325, 2023, 2026, 2830
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 2 cut(s) 288, 1844
Bme18I GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
BmeT110I CYCGRG 1 cut(s) 462
BmgBI CACGTC 4 cut(s) 109, 1363, 2936, 3047
BmgT120I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
BmiI GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
BmrFI CCNGG 2 cut(s) 288, 1844
BmrI ACTGGG 2 cut(s) 414, 2635
BmsI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
BmtI GCTAGC 1 cut(s) 1356
BmuI ACTGGG 2 cut(s) 414, 2635
BpiI GAAGAC 1 cut(s) 860
BpmI CTGGAG 1 cut(s) 1826
Bpu10I CCTNAGC 1 cut(s) 2100
BpuEI CTTGAG 2 cut(s) 1629, 1957
Bsa29I ATCGAT 1 cut(s) 1893
BsaBI GATNNNNATC 1 cut(s) 831
BsaI GGTCTC 2 cut(s) 2541, 2819
BsaJI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
Bsc4I CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
Bse1I ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
Bse3DI GCAATG 2 cut(s) 52, 2548
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 2 cut(s) 288, 1844
BseCI ATCGAT 1 cut(s) 1893
BseDI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
BseGI GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
BseMI GCAATG 2 cut(s) 52, 2548
BseMII CTCAG 9 cut(s) 654, 1128, 1634, 1844, 1950, 2114, 2424, 2760, 3056
BseNI ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
BseRI GAGGAG 1 cut(s) 1976
BseSI GKGCMC 2 cut(s) 1579, 2899
BseXI GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BseYI CCCAGC 1 cut(s) 1106
BsgI GTGCAG 1 cut(s) 85
BshFI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BshNI GGYRCC 1 cut(s) 3069
BshVI ATCGAT 1 cut(s) 1893
BsiHKAI GWGCWC 2 cut(s) 469, 1579
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 2 cut(s) 1327, 2080
BslFI GGGAC 4 cut(s) 594, 778, 863, 1385
BslI CCNNNNNNNGG 7 cut(s) 1112, 1214, 2246, 2336, 2558, 2612, 3079
BsmAI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BsmBI CGTCTC 1 cut(s) 2942
BsmFI GGGAC 4 cut(s) 594, 778, 863, 1385
BsnI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
Bso31I GGTCTC 2 cut(s) 2541, 2819
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 4 cut(s) 469, 1579, 2899, 3038
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
BspANI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BspCNI CTCAG 9 cut(s) 655, 1129, 1633, 1845, 1951, 2113, 2425, 2761, 3057
BspDI ATCGAT 1 cut(s) 1893
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 1 cut(s) 1356
BspPI GGATC 6 cut(s) 24, 834, 1816, 2231, 2759, 3069
BspQI GCTCTTC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 3069
BspTNI GGTCTC 2 cut(s) 2541, 2819
BsrDI GCAATG 2 cut(s) 52, 2548
BsrI ACTGG 5 cut(s) 420, 1353, 2539, 2641, 2653
BssECI CCNNGG 5 cut(s) 299, 757, 979, 1825, 2809
BssNAI GTATAC 1 cut(s) 2693
BssT1I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Bst1107I GTATAC 1 cut(s) 2693
Bst2UI CCWGG 2 cut(s) 288, 1844
Bst6I CTCTTC 4 cut(s) 343, 888, 1484, 1514
BstC8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BstHHI GCGC 2 cut(s) 1872, 2076
BstMAI GTCTC 7 cut(s) 43, 615, 793, 2541, 2730, 2819, 2942
BstMWI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
BstNI CCWGG 2 cut(s) 288, 1844
BstNSI RCATGY 4 cut(s) 458, 2328, 2656, 2835
BstSCI CCNGG 2 cut(s) 286, 1842
BstSLI GKGCMC 2 cut(s) 1579, 2899
BstV1I GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
BstV2I GAAGAC 1 cut(s) 860
BstX2I RGATCY 3 cut(s) 826, 2236, 3061
BstXI CCANNNNNNTGG 1 cut(s) 2648
BstYI RGATCY 3 cut(s) 826, 2236, 3061
BstZ17I GTATAC 1 cut(s) 2693
Bsu15I ATCGAT 1 cut(s) 1893
BsuI GTATCC 1 cut(s) 2944
BsuRI GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
BsuTUI ATCGAT 1 cut(s) 1893
BtgI CCRYGG 1 cut(s) 757
BtrI CACGTC 4 cut(s) 109, 1363, 2936, 3047
BtsCI GGATG 5 cut(s) 77, 921, 1093, 1276, 2345
BtsI GCAGTG 2 cut(s) 676, 796
BtsIMutI CAGTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2760, 2961
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 8 cut(s) 275, 686, 787, 1209, 1354, 1968, 2029, 2085
CciI TCATGA 1 cut(s) 25
CfoI GCGC 2 cut(s) 1872, 2076
Cfr13I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
ClaI ATCGAT 1 cut(s) 1893
CseI GACGC 1 cut(s) 348
DraI TTTAAA 1 cut(s) 1080
EaeI YGGCCR 2 cut(s) 2214, 2269
Eam1104I CTCTTC 4 cut(s) 343, 888, 1484, 1514
EarI CTCTTC 4 cut(s) 343, 888, 1484, 1514
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Eco24I GRGCYC 1 cut(s) 3038
Eco31I GGTCTC 2 cut(s) 2541, 2819
Eco47I GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
Eco57I CTGAAG 5 cut(s) 171, 1092, 1362, 2265, 2895
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 4 cut(s) 552, 610, 976, 2249
EcoRI GAATTC 2 cut(s) 130, 2428
EcoRII CCWGG 2 cut(s) 286, 1842
EcoT14I CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
EcoT22I ATGCAT 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 3038
ErhI CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
Esp3I CGTCTC 1 cut(s) 2942
FaqI GGGAC 4 cut(s) 594, 778, 863, 1385
FauI CCCGC 1 cut(s) 1207
FauNDI CATATG 4 cut(s) 355, 1378, 2017, 2116
FbaI TGATCA 2 cut(s) 970, 1147
FblI GTMKAC 1 cut(s) 2692
Fnu4HI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
FokI GGATG 5 cut(s) 84, 928, 1100, 1283, 2352
FriOI GRGCYC 1 cut(s) 3038
Fsp4HI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
GlaI GCGC 2 cut(s) 1871, 2075
GluI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 1 cut(s) 1826
HaeIII GGCC 8 cut(s) 490, 612, 684, 978, 1207, 2216, 2250, 2271
HapII CCGG 2 cut(s) 1327, 2080
HgaI GACGC 1 cut(s) 348
HhaI GCGC 2 cut(s) 1872, 2076
Hin6I GCGC 2 cut(s) 1870, 2074
HinP1I GCGC 2 cut(s) 1870, 2074
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 2 cut(s) 1074, 2085
HpaII CCGG 2 cut(s) 1327, 2080
HphI GGTGA 6 cut(s) 412, 782, 1615, 2324, 2595, 3029
Hpy166II GTNNAC 4 cut(s) 174, 1577, 2646, 2693
Hpy8I GTNNAC 4 cut(s) 174, 1577, 2646, 2693
HpyAV CCTTC 7 cut(s) 187, 348, 566, 1677, 1679, 2240, 2697
HpyCH4IV ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
HpyF10VI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
HpySE526I ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
HspAI GCGC 2 cut(s) 1870, 2074
KpnI GGTACC 1 cut(s) 3073
Ksp22I TGATCA 2 cut(s) 970, 1147
LguI GCTCTTC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 1264, 1845, 1963
Lsp1109I GCAGC 8 cut(s) 445, 583, 827, 1223, 1284, 1335, 2011, 2815
LweI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
MaeII ACGT 5 cut(s) 108, 333, 1362, 2935, 3046
MfeI CAATTG 3 cut(s) 167, 1719, 2451
MflI RGATCY 3 cut(s) 826, 2236, 3061
MhlI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
MlsI TGGCCA 2 cut(s) 2216, 2271
MluNI TGGCCA 2 cut(s) 2216, 2271
MlyI GAGTC 3 cut(s) 2114, 2157, 2731
MmeI TCCRAC 2 cut(s) 2637, 2891
Mox20I TGGCCA 2 cut(s) 2216, 2271
Mph1103I ATGCAT 1 cut(s) 705
MscI TGGCCA 2 cut(s) 2216, 2271
MslI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
Msp20I TGGCCA 2 cut(s) 2216, 2271
MspA1I CMGCKG 3 cut(s) 1110, 1214, 2024
MspI CCGG 2 cut(s) 1327, 2080
MspR9I CCNGG 2 cut(s) 288, 1844
MunI CAATTG 3 cut(s) 167, 1719, 2451
MvaI CCWGG 2 cut(s) 288, 1844
MwoI GCNNNNNNNGC 6 cut(s) 464, 568, 1289, 2021, 2080, 2598
NcoI CCATGG 1 cut(s) 757
NdeI CATATG 4 cut(s) 355, 1378, 2017, 2116
NheI GCTAGC 1 cut(s) 1352
NlaIV GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
NmuCI GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 4 cut(s) 458, 2328, 2656, 2835
OliI CACNNNNGTG 2 cut(s) 107, 398
PaeR7I CTCGAG 1 cut(s) 462
PagI TCATGA 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 1817
PflFI GACNNNGTC 1 cut(s) 2129
PflMI CCANNNNNTGG 1 cut(s) 2612
PkrI GCNGC 9 cut(s) 460, 573, 817, 1213, 1299, 1325, 2023, 2026, 2830
PleI GAGTC 3 cut(s) 2114, 2157, 2730
PpsI GAGTC 3 cut(s) 2114, 2157, 2730
PpuMI RGGWCCY 1 cut(s) 552
PshBI ATTAAT 1 cut(s) 384
PsiI TTATAA 2 cut(s) 516, 1833
Psp5II RGGWCCY 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 286, 1842
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 2 cut(s) 286, 1842
PspN4I GGNNCC 6 cut(s) 554, 582, 683, 1803, 2563, 3071
PspPI GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
PspPPI RGGWCCY 1 cut(s) 552
PspXI VCTCGAGB 1 cut(s) 462
PsuI RGATCY 3 cut(s) 826, 2236, 3061
PsyI GACNNNGTC 1 cut(s) 2129
RseI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
SapI GCTCTTC 1 cut(s) 343
SatI GCNGC 9 cut(s) 459, 572, 816, 1212, 1298, 1324, 2022, 2025, 2829
Sau96I GGNCC 9 cut(s) 284, 552, 581, 610, 682, 976, 2249, 2615, 2851
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 3 cut(s) 2114, 2157, 2731
ScrFI CCNGG 2 cut(s) 288, 1844
SduI GDGCHC 4 cut(s) 469, 1579, 2899, 3038
SfaNI GCATC 7 cut(s) 906, 2055, 2203, 2323, 2681, 2738, 3014
Sfr274I CTCGAG 1 cut(s) 462
SinI GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
SlaI CTCGAG 1 cut(s) 462
SmiMI CAYNNNNRTG 5 cut(s) 24, 107, 398, 995, 2780
SmlI CTYRAG 3 cut(s) 462, 1644, 1936
SmoI CTYRAG 3 cut(s) 462, 1644, 1936
SsiI CCGC 7 cut(s) 14, 389, 560, 1110, 1214, 1566, 2022
SspI AATATT 3 cut(s) 382, 520, 2056
StyD4I CCNGG 2 cut(s) 286, 1842
StyI CCWWGG 5 cut(s) 299, 757, 979, 1825, 2809
TaiI ACGT 5 cut(s) 111, 336, 1365, 2938, 3049
TaqI TCGA 9 cut(s) 451, 463, 1156, 1249, 1672, 1811, 1820, 1893, 3057
TatI WGTACW 5 cut(s) 587, 1175, 1462, 2321, 2697
TauI GCSGC 1 cut(s) 2024
TscAI CASTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2767, 2961
TseFI GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
TseI GCWGC 8 cut(s) 458, 571, 815, 1211, 1297, 1323, 2024, 2828
Tsp45I GTSAC 5 cut(s) 743, 770, 2090, 2511, 2790
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 8 cut(s) 676, 796, 1259, 2040, 2294, 2648, 2767, 2961
Tth111I GACNNNGTC 1 cut(s) 2129
Van91I CCANNNNNTGG 1 cut(s) 2612
VneI GTGCAC 1 cut(s) 1575
VpaK11BI GGWCC 5 cut(s) 284, 552, 581, 2615, 2851
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 8 cut(s) 130, 194, 409, 510, 650, 1081, 1816, 2428
XbaI TCTAGA 2 cut(s) 326, 1046
XceI RCATGY 4 cut(s) 458, 2328, 2656, 2835
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XhoI CTCGAG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 2692
ZrmI AGTACT 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.