pycom16g00620

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010092
Physical Location & Seq
Forward (+)
375166 .. 377647
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1479 bp
ATGGCGTCTTCGAGTAAATCTTTGAAAGAGTATTTGAAACGGTATGAAGTGAATAATGAAGAGGATAAGAAGAAAAAGAAGAAGAACAAGAAGCAGAAAACCAAGCCGGAGCCAATGGGCGTTTTAGTGGTTGATGAAGATCCTGTTTGGCAGAAGCCGGTGAATGTTGAGGAAGATAATGGTGACAATTCATCGGATGAAGACAAACCTGTAGTTGATGAGGACATTGAGATTAAGAGGATGAAGAGACTTCAACAATTGAGGGCCAGACGTCCCTTTAATTCCATATCCGAAGATGGAAGCGGTTGGGTTACAGTTGGAGACTCTCCTCCGAAGCAGTCAAAGTCTGATTTATCTCCACCTCGTAGGGAGAGGAGTTTGGTTCCGAACTGTGAACCAATGTCTCCGGAAAATAGGAGGAAGGATGCTGACTTGTCACCTCCACGGCAGAGACGGAAGCGCCACCACACCCCGTCACCCGAGCCTGAAACGAACCACGAGTACTTGCCTCCTCGTCCTGGATCAGATCTTTCACCCCCAAGGAAAAGGAGGCAGGACGTTGATGCAGATCTGTCTCCACCAAGGAAGAGCAGGAAAGATAGTAATGAAGATCTGTCTCCACCAAGGAAGGGCAGGAAGTACGCTGATGCAGATCTTTCTCCACCAAGGAAAAGTAGGAACAATGTTGATGCTGATCTTTCTCCACCGAGGAAAAGTAGGAACAATTTTGATGCTGATCTTTCTCCACCAAGGAATAGCAGGAGGGATGTTGATACTGATCTTTCCCCACCAAGGAAAAGCAGGAAGGATGTTGATGGTGATCTTTCCCCACCAAGGAATAAAAAGCAAGTTACTCCGCACCCTCGAAAAGAGCAAGCAAAAGTTGGTCTGATCTCTGGCCGAGAGATTAGTGACGAGATCAATAAAACGAAGAAGGATGAGTGGTTAAGATTTCAACGCATGGATCCTTCTGTAAGTGGGAGAGAGGCAGTACCTATATATCGTGACAAGAAAACAGGGGAACGCATTTCGAAGGAGGAGCTTTTGAAATCGCAAAGGAAAATAGATGAAAAACCAAAGGAGATAAAGTTGGAATGGGGCAAAGGCTTGGCCCAAAAGCGAGAAGCTGAAACTAAGCTGGAGGAATTAGAACTTGAGAAGGATAAGCCTTTTGCACGGACTAGAGATGATCCTGAACTCGACAAAATATTGAAGGAGAGAACAAGGTGGGGCGATCCCATGGCACATTTGGTAAAGAAAAAGCATTCCGAGCCAGTTCTCTCTAACTTGGGGGATGATGAGAAAATGAAGGAATCTGGATTCATTATTCCCCAGGATATTCCTGTTCACAGCTGGTTAAAAAGAGGATTGGATGCTGCACCAAATCGATACGGATTTGAGAAGGGAATGTTCAAAAGGAAGAACGAGAAACAAGCCACGGAGGCAGAAGCTTATCTTTGGTCTGTGGCGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

493

Amino Acids

56.84

Weight (kDa)

9.52

Isoelectric Point (pI)

62.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 333 - 469 7.4e-24 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 274
AccIII TCCGGA 1 cut(s) 406
AciI CCGC 3 cut(s) 303, 857, 1469
AclWI GGATC 6 cut(s) 134, 529, 959, 972, 1184, 1229
AcoI YGGCCR 1 cut(s) 898
AcyI GRCGYC 2 cut(s) 5, 271
AfaI GTAC 3 cut(s) 503, 641, 993
AfiI CCNNNNNNNGG 4 cut(s) 518, 629, 792, 834
AgsI TTSAA 7 cut(s) 25, 37, 254, 956, 1048, 1213, 1414
AjnI CCWGG 2 cut(s) 517, 1332
AluBI AGCT 5 cut(s) 1042, 1127, 1138, 1353, 1451
AluI AGCT 5 cut(s) 1042, 1127, 1138, 1353, 1451
Alw26I GTCTC 6 cut(s) 241, 315, 408, 445, 579, 621
AlwI GGATC 6 cut(s) 134, 529, 959, 972, 1184, 1229
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 406
AoxI GGCC 3 cut(s) 264, 898, 1110
ApeKI GCWGC 1 cut(s) 1376
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 264, 1111
AsuHPI GGTGA 6 cut(s) 172, 194, 429, 468, 525, 830
AsuII TTCGAA 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 479
BamHI GGATCC 1 cut(s) 964
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 1363
BccI CCATC 2 cut(s) 290, 809
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 2 cut(s) 519, 1334
BcoDI GTCTC 6 cut(s) 241, 315, 408, 445, 579, 621
BfaI CTAG 1 cut(s) 1182
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 1442
BglII AGATCT 4 cut(s) 526, 568, 610, 652
BisI GCNGC 1 cut(s) 1377
BlsI GCNGC 1 cut(s) 1378
BmcAI AGTACT 1 cut(s) 503
Bme1390I CCNGG 2 cut(s) 519, 1334
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 264, 1111
BmiI GGNNCC 3 cut(s) 111, 384, 966
BmrFI CCNGG 2 cut(s) 519, 1334
BmsI GCATC 6 cut(s) 415, 553, 637, 679, 721, 1363
BpiI GAAGAC 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 1160
Bpu14I TTCGAA 1 cut(s) 1031
BpuEI CTTGAG 1 cut(s) 1175
Bsa29I ATCGAT 1 cut(s) 1387
BsaBI GATNNNNATC 7 cut(s) 138, 567, 651, 693, 735, 777, 819
BsaHI GRCGYC 2 cut(s) 5, 271
BsaWI WCCGGW 1 cut(s) 406
BsaXI ACNNNNNCTCC 2 cut(s) 1073, 1103
Bsc4I CCNNNNNNNGG 4 cut(s) 518, 629, 792, 834
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 1274
Bse8I GATNNNNATC 7 cut(s) 138, 567, 651, 693, 735, 777, 819
BseAI TCCGGA 1 cut(s) 406
BseBI CCWGG 2 cut(s) 519, 1334
BseCI ATCGAT 1 cut(s) 1387
BseGI GGATG 8 cut(s) 202, 246, 430, 772, 814, 943, 1300, 1378
BseJI GATNNNNATC 7 cut(s) 138, 567, 651, 693, 735, 777, 819
BseLI CCNNNNNNNGG 4 cut(s) 518, 629, 792, 834
BseNI ACTGG 1 cut(s) 1274
BseRI GAGGAG 4 cut(s) 318, 388, 501, 1052
BseXI GCAGC 1 cut(s) 1363
BsgI GTGCAG 1 cut(s) 1362
BshFI GGCC 3 cut(s) 266, 900, 1112
BshVI ATCGAT 1 cut(s) 1387
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 3 cut(s) 107, 158, 407
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 4 cut(s) 518, 629, 792, 834
BsmAI GTCTC 6 cut(s) 241, 315, 408, 445, 579, 621
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 1264
BsnI GGCC 3 cut(s) 266, 900, 1112
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 1 cut(s) 1031
Bsp13I TCCGGA 1 cut(s) 406
Bsp19I CCATGG 1 cut(s) 1239
BspACI CCGC 3 cut(s) 303, 857, 1469
BspANI GGCC 3 cut(s) 266, 900, 1112
BspDI ATCGAT 1 cut(s) 1387
BspEI TCCGGA 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 111, 384, 966
BspPI GGATC 6 cut(s) 134, 529, 959, 972, 1184, 1229
BspQI GCTCTTC 1 cut(s) 581
BspT104I TTCGAA 1 cut(s) 1031
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 1274
BssAI RCCGGY 1 cut(s) 157
BssNI GRCGYC 2 cut(s) 5, 271
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 8 cut(s) 539, 581, 623, 665, 749, 791, 833, 1239
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 2 cut(s) 519, 1334
Bst4CI ACNGT 3 cut(s) 42, 316, 392
Bst6I CTCTTC 3 cut(s) 54, 239, 581
BstACI GRCGYC 2 cut(s) 5, 271
BstBI TTCGAA 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 876
BstDEI CTNAG 1 cut(s) 1134
BstDSI CCRYGG 3 cut(s) 443, 1239, 1437
BstF5I GGATG 8 cut(s) 202, 246, 430, 772, 814, 943, 1300, 1378
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstMAI GTCTC 6 cut(s) 241, 315, 408, 445, 579, 621
BstMWI GCNNNNNNNGC 2 cut(s) 1270, 1442
BstNI CCWGG 2 cut(s) 519, 1334
BstSCI CCNGG 2 cut(s) 517, 1332
BstSFI CTRYAG 1 cut(s) 210
BstV1I GCAGC 1 cut(s) 1363
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 6 cut(s) 139, 526, 568, 610, 652, 964
BstYI RGATCY 6 cut(s) 139, 526, 568, 610, 652, 964
Bsu15I ATCGAT 1 cut(s) 1387
BsuRI GGCC 3 cut(s) 266, 900, 1112
BsuTUI ATCGAT 1 cut(s) 1387
BtgI CCRYGG 3 cut(s) 443, 1239, 1437
BtsCI GGATG 8 cut(s) 202, 246, 430, 772, 814, 943, 1300, 1378
Cac8I GCNNGC 1 cut(s) 876
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 264, 1111
ClaI ATCGAT 1 cut(s) 1387
Csp6I GTAC 3 cut(s) 502, 640, 992
CviAII CATG 2 cut(s) 961, 1240
CviQI GTAC 3 cut(s) 502, 640, 992
DdeI CTNAG 1 cut(s) 1134
EaeI YGGCCR 1 cut(s) 898
Eam1104I CTCTTC 3 cut(s) 54, 239, 581
EarI CTCTTC 3 cut(s) 54, 239, 581
Eco130I CCWWGG 8 cut(s) 539, 581, 623, 665, 749, 791, 833, 1239
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 2 cut(s) 517, 1332
EcoT14I CCWWGG 8 cut(s) 539, 581, 623, 665, 749, 791, 833, 1239
ErhI CCWWGG 8 cut(s) 539, 581, 623, 665, 749, 791, 833, 1239
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 2 cut(s) 964, 1243
FaiI YATR 7 cut(s) 45, 287, 962, 998, 1000, 1241, 1475
FalI AAGNNNNNCTT 2 cut(s) 1440, 1472
FaqI GGGAC 1 cut(s) 258
FatI CATG 2 cut(s) 960, 1239
Fnu4HI GCNGC 1 cut(s) 1377
FokI GGATG 8 cut(s) 209, 253, 437, 779, 821, 950, 1307, 1385
Fsp4HI GCNGC 1 cut(s) 1377
FspBI CTAG 1 cut(s) 1182
GlaI GCGC 1 cut(s) 461
GluI GCNGC 1 cut(s) 1377
GsuI CTGGAG 1 cut(s) 1160
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 3 cut(s) 266, 900, 1112
HapII CCGG 3 cut(s) 107, 158, 407
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 2 cut(s) 5, 271
Hin1II CATG 2 cut(s) 964, 1243
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 1449
HinfI GANTC 3 cut(s) 323, 1313, 1320
HpaII CCGG 3 cut(s) 107, 158, 407
HphI GGTGA 6 cut(s) 172, 194, 429, 468, 525, 830
Hpy166II GTNNAC 2 cut(s) 395, 1348
Hpy188I TCNGA 8 cut(s) 196, 292, 333, 349, 387, 526, 891, 1270
Hpy188III TCNNGA 4 cut(s) 407, 1004, 1193, 1317
Hpy8I GTNNAC 2 cut(s) 395, 1348
HpyCH4III ACNGT 3 cut(s) 42, 316, 392
HpyCH4IV ACGT 2 cut(s) 271, 558
HpyCH4V TGCA 4 cut(s) 566, 650, 1175, 1379
HpyF10VI GCNNNNNNNGC 2 cut(s) 1270, 1442
HpyF3I CTNAG 1 cut(s) 1134
HpySE526I ACGT 2 cut(s) 271, 558
Hsp92I GRCGYC 2 cut(s) 5, 271
Hsp92II CATG 2 cut(s) 964, 1243
HspAI GCGC 1 cut(s) 460
Kpn2I TCCGGA 1 cut(s) 406
LguI GCTCTTC 1 cut(s) 581
LmnI GCTCC 2 cut(s) 109, 1039
Lsp1109I GCAGC 1 cut(s) 1363
LweI GCATC 6 cut(s) 415, 553, 637, 679, 721, 1363
MaeI CTAG 1 cut(s) 1182
MaeII ACGT 2 cut(s) 271, 558
MaeIII GTNAC 7 cut(s) 182, 310, 435, 474, 850, 911, 1004
MfeI CAATTG 1 cut(s) 257
MflI RGATCY 6 cut(s) 139, 526, 568, 610, 652, 964
MluCI AATT 5 cut(s) 187, 257, 280, 724, 1145
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 298, 1071
MroI TCCGGA 1 cut(s) 406
MseI TTAA 4 cut(s) 234, 279, 947, 1358
MspA1I CMGCKG 1 cut(s) 1353
MspI CCGG 3 cut(s) 107, 158, 407
MspR9I CCNGG 2 cut(s) 519, 1334
MunI CAATTG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 1264
MvaI CCWGG 2 cut(s) 519, 1334
MwoI GCNNNNNNNGC 2 cut(s) 1270, 1442
NcoI CCATGG 1 cut(s) 1239
NlaIII CATG 2 cut(s) 964, 1243
NlaIV GGNNCC 3 cut(s) 111, 384, 966
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 5 cut(s) 182, 435, 474, 911, 1004
NspV TTCGAA 1 cut(s) 1031
PciSI GCTCTTC 1 cut(s) 581
PctI GAATGC 1 cut(s) 1264
PfeI GAWTC 2 cut(s) 1313, 1320
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 1 cut(s) 1378
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 517, 1332
PspGI CCWGG 2 cut(s) 517, 1332
PspN4I GGNNCC 3 cut(s) 111, 384, 966
PspPI GGNCC 2 cut(s) 264, 1111
PsrI GAACNNNNNNTAC 2 cut(s) 485, 517
PsuI RGATCY 6 cut(s) 139, 526, 568, 610, 652, 964
PvuII CAGCTG 1 cut(s) 1353
RsaI GTAC 3 cut(s) 503, 641, 993
RsaNI GTAC 3 cut(s) 502, 640, 992
SapI GCTCTTC 1 cut(s) 581
SaqAI TTAA 4 cut(s) 234, 279, 947, 1358
SatI GCNGC 1 cut(s) 1377
Sau96I GGNCC 2 cut(s) 264, 1111
ScaI AGTACT 1 cut(s) 503
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 2 cut(s) 519, 1334
SfaNI GCATC 6 cut(s) 415, 553, 637, 679, 721, 1363
SfcI CTRYAG 1 cut(s) 210
SfuI TTCGAA 1 cut(s) 1031
SmlI CTYRAG 1 cut(s) 1154
SmoI CTYRAG 1 cut(s) 1154
Sse9I AATT 5 cut(s) 187, 257, 280, 724, 1145
SsiI CCGC 3 cut(s) 303, 857, 1469
SspI AATATT 1 cut(s) 1209
SspMI CTAG 1 cut(s) 1182
StyD4I CCNGG 2 cut(s) 517, 1332
StyI CCWWGG 8 cut(s) 539, 581, 623, 665, 749, 791, 833, 1239
TaaI ACNGT 3 cut(s) 42, 316, 392
TaiI ACGT 2 cut(s) 274, 561
TaqI TCGA 5 cut(s) 11, 865, 1031, 1200, 1387
TasI AATT 5 cut(s) 187, 257, 280, 724, 1145
TatI WGTACW 1 cut(s) 501
TfiI GAWTC 2 cut(s) 1313, 1320
Tru1I TTAA 4 cut(s) 234, 279, 947, 1358
Tru9I TTAA 4 cut(s) 234, 279, 947, 1358
TseFI GTSAC 5 cut(s) 182, 435, 474, 911, 1004
TseI GCWGC 1 cut(s) 1376
Tsp45I GTSAC 5 cut(s) 182, 435, 474, 911, 1004
TspGWI ACGGA 4 cut(s) 469, 1192, 1407, 1454
XcmI CCANNNNNNNNNTGG 1 cut(s) 1246
XspI CTAG 1 cut(s) 1182
ZraI GACGTC 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.