pycom16g01990

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011757
Physical Location & Seq
Reverse (-)
1249048 .. 1250770
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1419 bp
ATGGCTGCCAACAAATTCGCAACCATGCTGCACAGAAACACCAACAAGATCACTCTCGTTCTAATCTATGCACTCCTCGAATGGATTCTGATCATCCTCCTCCTTCTGAATTCCCTATTTTCTTATCTGATCCTAAAGTTTTCCGATTACTTTGGCCTCAAAAGACCCTGCCTCTGGTGTTCCAGGCTCGATCATATCTTCCACCCCAGAAACACCAACAATTCTTACAGAGATCTTGTCTGCGAATCTCATGCCAACGAAATATCCCAACTGGGTTATTGCTCAAATCACCAGAAACTAGCAGAATTGCAAGATATGTGCGAGGACTGCTCGTCGAAGCCGGATTGCGAAGAATGGTCAAAGAAGTTTGCTTTTTTTCCATGGATGAAGCAAATTGGGATGATTCGAGACGGCGACGAAAAAGTCAATGAAAATGGAGACGAGAAGTTGAACTGTTCTTGCTGCGGATTGAGCTTGAACAAGTTTTACCCTTCTTGTATTGTGATTAAACCCTCTTGGGAGGTTTTGGATTATGCCCAGAAACAGAATTTGAGTTTGGAAACAGGGGATGATCGATCCCGACGTCCAAATAAACATAATGCGGAAGAAGGTGATCGGTCGGATCGAAGCGGATCGGATTTCGAGATCGGAAACAAAGAGGGAGTTGAAGAAAAGAAGGGAGAAAATAGGGGTTTTGAAGCAGAGGATCATTCTGCTGCTTGTTCTGTATGTGACTATGGCTGCAAGGAGGAGATTGCAGCCAATGGAGATGAAGAAGTGGAGAGAGTCATAGAAGAAGAACAAGGGCCCACGAAAGATATTGTATCTCCAAAAATTCGACCTCAGGATTTGGAGTTCTACATCGATCAGGACGATAGTGGGTTGGTTCTGGTTGATTTGATTGATTCTCCAACCACCATTGGAAACCAGAGTAACCAGAAGTATAATAAGGTGGAAGAAGACCAAGGAAATAGTAGTGGTTGGGAAGATGTTATACTGGATTTCGACATTGGCTTCGAGAGACAAGAAGCGAAACCGGTTGTGGAGAGTTGGCGCGGTCCAGAAAAGTCTGAGGCATTGCTTTCGTTCGATGAGAGTAAGGAGGAGGAAAGTTGGGTTTCGGGTCTTGATTCGAAGGGTTCGGACGAAACTGAGGGCGCAAAGGAGGAAAATGAACAAGTTGGAGCTATAGCAAATGGTGAAATTGTTTCAGATGTTCATCAGGAATTTGAGGACGATGTCCTTTCGGGCGACGACGAGATTGATGCAGAGGTCTCAATAGGAACAGAGATTCCTGATCAGGAAGAGAATGATGAAGTTCAATCAGCTCAAGAACTTTTGTATTCGTATGCGTGTGAGCAGGAAGACCCTTCTACAAGTTCTGCCAATCTACATGATTGTGATCGATCATGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

473

Amino Acids

53.43

Weight (kDa)

4.34

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AatII GACGTC 1 cut(s) 586
AccII CGCG 1 cut(s) 1056
AciI CCGC 4 cut(s) 465, 602, 630, 1056
AclWI GGATC 5 cut(s) 124, 570, 630, 640, 714
AcsI RAATTY 5 cut(s) 14, 109, 547, 834, 1226
AcyI GRCGYC 1 cut(s) 583
AfiI CCNNNNNNNGG 1 cut(s) 174
AgeI ACCGGT 1 cut(s) 1036
AgsI TTSAA 5 cut(s) 451, 478, 668, 698, 1322
AhdI GACNNNNNGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 182
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AloI GAACNNNNNNTCC 4 cut(s) 839, 871, 1276, 1308
AluBI AGCT 3 cut(s) 474, 1187, 1328
AluI AGCT 3 cut(s) 474, 1187, 1328
Alw26I GTCTC 4 cut(s) 402, 432, 1015, 1279
AlwI GGATC 5 cut(s) 124, 570, 630, 640, 714
AoxI GGCC 2 cut(s) 154, 806
ApaI GGGCCC 1 cut(s) 810
ApeKI GCWGC 6 cut(s) 5, 28, 462, 716, 741, 758
ApoI RAATTY 5 cut(s) 14, 109, 547, 834, 1226
ArsI GACNNNNNNTTYG 2 cut(s) 998, 1030
AsiGI ACCGGT 1 cut(s) 1036
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 2 cut(s) 1056, 1160
AspS9I GGNCC 3 cut(s) 806, 807, 1058
AsuHPI GGTGA 3 cut(s) 281, 623, 1211
AsuII TTCGAA 1 cut(s) 1133
AvaII GGWCC 1 cut(s) 1058
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 810
BarI GAAGNNNNNNTAC 2 cut(s) 978, 1010
BbsI GAAGAC 2 cut(s) 966, 1371
BbvI GCAGC 5 cut(s) 15, 449, 703, 728, 770
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 6 cut(s) 233, 267, 1065, 1099, 1247, 1281
BciT130I CCWGG 1 cut(s) 184
BclI TGATCA 2 cut(s) 90, 1297
BcoDI GTCTC 4 cut(s) 402, 432, 1015, 1279
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 1188
BglII AGATCT 1 cut(s) 232
BisI GCNGC 6 cut(s) 6, 29, 463, 717, 742, 759
BlsI GCNGC 6 cut(s) 7, 30, 464, 718, 743, 760
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 1058
BmeRI GACNNNNNGTC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 806, 807, 1058
BmiI GGNNCC 1 cut(s) 808
BmrFI CCNGG 1 cut(s) 184
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 1 cut(s) 1255
BmuI ACTGGG 1 cut(s) 281
BpiI GAAGAC 2 cut(s) 966, 1371
BplI GAGNNNNNCTC 2 cut(s) 314, 346
Bpu14I TTCGAA 1 cut(s) 1133
BpuEI CTTGAG 1 cut(s) 1314
Bsa29I ATCGAT 3 cut(s) 574, 864, 1405
BsaBI GATNNNNATC 3 cut(s) 89, 1218, 1401
BsaHI GRCGYC 1 cut(s) 583
BsaI GGTCTC 1 cut(s) 1279
BsaJI CCNNGG 2 cut(s) 380, 964
BsaWI WCCGGW 1 cut(s) 1036
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse118I RCCGGY 1 cut(s) 1036
Bse1I ACTGG 2 cut(s) 276, 1002
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 1076
Bse8I GATNNNNATC 3 cut(s) 89, 1218, 1401
BseBI CCWGG 1 cut(s) 184
BseCI ATCGAT 3 cut(s) 574, 864, 1405
BseDI CCNNGG 2 cut(s) 380, 964
BseGI GGATG 4 cut(s) 93, 390, 405, 574
BseJI GATNNNNATC 3 cut(s) 89, 1218, 1401
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 857, 1062, 1143
BseNI ACTGG 2 cut(s) 276, 1002
BseRI GAGGAG 4 cut(s) 65, 89, 764, 1118
BseSI GKGCMC 1 cut(s) 810
BseXI GCAGC 5 cut(s) 15, 449, 703, 728, 770
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 1056
Bsh1285I CGRYCG 1 cut(s) 620
BshFI GGCC 2 cut(s) 156, 808
BshTI ACCGGT 1 cut(s) 1036
BshVI ATCGAT 3 cut(s) 574, 864, 1405
BsiEI CGRYCG 1 cut(s) 620
BsiSI CCGG 2 cut(s) 341, 1037
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 4 cut(s) 402, 432, 1015, 1279
BsmBI CGTCTC 2 cut(s) 402, 432
BsnI GGCC 2 cut(s) 156, 808
Bso31I GGTCTC 1 cut(s) 1279
Bsp119I TTCGAA 1 cut(s) 1133
Bsp120I GGGCCC 1 cut(s) 806
Bsp1286I GDGCHC 1 cut(s) 810
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 4 cut(s) 465, 602, 630, 1056
BspANI GGCC 2 cut(s) 156, 808
BspCNI CTCAG 3 cut(s) 856, 1063, 1144
BspDI ATCGAT 3 cut(s) 574, 864, 1405
BspFNI CGCG 1 cut(s) 1056
BspLI GGNNCC 1 cut(s) 808
BspPI GGATC 5 cut(s) 124, 570, 630, 640, 714
BspT104I TTCGAA 1 cut(s) 1133
BspTNI GGTCTC 1 cut(s) 1279
BsrDI GCAATG 1 cut(s) 1076
BsrFI RCCGGY 1 cut(s) 1036
BsrI ACTGG 2 cut(s) 276, 1002
BssAI RCCGGY 1 cut(s) 1036
BssECI CCNNGG 2 cut(s) 380, 964
BssNI GRCGYC 1 cut(s) 583
BssT1I CCWWGG 2 cut(s) 380, 964
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 1 cut(s) 1299
BstACI GRCGYC 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 1133
BstDEI CTNAG 3 cut(s) 843, 1071, 1152
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 4 cut(s) 93, 390, 405, 574
BstFNI CGCG 1 cut(s) 1056
BstHHI GCGC 2 cut(s) 1056, 1160
BstMAI GTCTC 4 cut(s) 402, 432, 1015, 1279
BstMCI CGRYCG 1 cut(s) 620
BstMWI GCNNNNNNNGC 2 cut(s) 327, 471
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 182
BstSFI CTRYAG 1 cut(s) 1188
BstSLI GKGCMC 1 cut(s) 810
BstUI CGCG 1 cut(s) 1056
BstV1I GCAGC 5 cut(s) 15, 449, 703, 728, 770
BstV2I GAAGAC 2 cut(s) 966, 1371
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu15I ATCGAT 3 cut(s) 574, 864, 1405
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 2 cut(s) 156, 808
BsuTUI ATCGAT 3 cut(s) 574, 864, 1405
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 4 cut(s) 93, 390, 405, 574
CfoI GCGC 2 cut(s) 1056, 1160
Cfr10I RCCGGY 1 cut(s) 1036
Cfr13I GGNCC 3 cut(s) 806, 807, 1058
ClaI ATCGAT 3 cut(s) 574, 864, 1405
CspAI ACCGGT 1 cut(s) 1036
CviAII CATG 5 cut(s) 25, 251, 381, 1394, 1410
DdeI CTNAG 3 cut(s) 843, 1071, 1152
DrdI GACNNNNNNGTC 1 cut(s) 422
DriI GACNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 1299
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 1299
Eco130I CCWWGG 2 cut(s) 380, 964
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 1279
Eco47I GGWCC 1 cut(s) 1058
Eco81I CCTNAGG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 182
EcoT14I CCWWGG 2 cut(s) 380, 964
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 2 cut(s) 380, 964
Esp3I CGTCTC 2 cut(s) 402, 432
FaeI CATG 5 cut(s) 28, 254, 384, 1397, 1413
FatI CATG 5 cut(s) 24, 250, 380, 1393, 1409
FbaI TGATCA 2 cut(s) 90, 1297
Fnu4HI GCNGC 6 cut(s) 6, 29, 463, 717, 742, 759
FokI GGATG 4 cut(s) 80, 397, 412, 581
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 6 cut(s) 6, 29, 463, 717, 742, 759
FspBI CTAG 1 cut(s) 299
GlaI GCGC 2 cut(s) 1055, 1159
GluI GCNGC 6 cut(s) 6, 29, 463, 717, 742, 759
HaeIII GGCC 2 cut(s) 156, 808
HapII CCGG 2 cut(s) 341, 1037
HhaI GCGC 2 cut(s) 1056, 1160
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 5 cut(s) 28, 254, 384, 1397, 1413
Hin6I GCGC 2 cut(s) 1054, 1158
HinP1I GCGC 2 cut(s) 1054, 1158
HinfI GANTC 7 cut(s) 85, 245, 403, 786, 905, 1130, 1291
HpaII CCGG 2 cut(s) 341, 1037
HphI GGTGA 3 cut(s) 281, 623, 1211
Hpy99I CGWCG 5 cut(s) 337, 419, 585, 1256, 1259
HpyAV CCTTC 6 cut(s) 113, 501, 602, 670, 1129, 1380
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 6 cut(s) 31, 71, 310, 744, 758, 1268
HpyF10VI GCNNNNNNNGC 2 cut(s) 327, 471
HpyF3I CTNAG 3 cut(s) 843, 1071, 1152
HpySE526I ACGT 1 cut(s) 583
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 5 cut(s) 28, 254, 384, 1397, 1413
HspAI GCGC 2 cut(s) 1054, 1158
Ksp22I TGATCA 2 cut(s) 90, 1297
LmnI GCTCC 1 cut(s) 1184
Lsp1109I GCAGC 5 cut(s) 15, 449, 703, 728, 770
LweI GCATC 1 cut(s) 1255
MaeI CTAG 1 cut(s) 299
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 2 cut(s) 731, 932
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 810
MluCI AATT 9 cut(s) 14, 109, 220, 305, 393, 547, 834, 1203, 1226
MlyI GAGTC 1 cut(s) 795
MmeI TCCRAC 3 cut(s) 600, 935, 1162
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 1398
MspI CCGG 2 cut(s) 341, 1037
MspR9I CCNGG 1 cut(s) 184
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 1056
MwoI GCNNNNNNNGC 2 cut(s) 327, 471
NcoI CCATGG 1 cut(s) 380
NlaIII CATG 5 cut(s) 28, 254, 384, 1397, 1413
NlaIV GGNNCC 1 cut(s) 808
NmuCI GTSAC 1 cut(s) 731
NspV TTCGAA 1 cut(s) 1133
PcsI WCGNNNNNNNCGW 3 cut(s) 580, 622, 870
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 6 cut(s) 85, 245, 403, 905, 1130, 1291
PflFI GACNNNGTC 1 cut(s) 1238
PinAI ACCGGT 1 cut(s) 1036
PkrI GCNGC 6 cut(s) 7, 30, 464, 718, 743, 760
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 1 cut(s) 808
PspOMI GGGCCC 1 cut(s) 806
PspPI GGNCC 3 cut(s) 806, 807, 1058
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PsuI RGATCY 1 cut(s) 232
PsyI GACNNNGTC 1 cut(s) 1238
RseI CAYNNNNRTG 1 cut(s) 1398
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 6 cut(s) 6, 29, 463, 717, 742, 759
Sau96I GGNCC 3 cut(s) 806, 807, 1058
SchI GAGTC 1 cut(s) 795
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 1 cut(s) 810
SetI ASST 9 cut(s) 476, 525, 586, 613, 844, 954, 1189, 1275, 1330
SfaNI GCATC 1 cut(s) 1255
SfcI CTRYAG 1 cut(s) 1188
SfuI TTCGAA 1 cut(s) 1133
SinI GGWCC 1 cut(s) 1058
SmiMI CAYNNNNRTG 1 cut(s) 1398
SmlI CTYRAG 1 cut(s) 1329
SmoI CTYRAG 1 cut(s) 1329
Sse9I AATT 9 cut(s) 14, 109, 220, 305, 393, 547, 834, 1203, 1226
SsiI CCGC 4 cut(s) 465, 602, 630, 1056
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 2 cut(s) 380, 964
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 1 cut(s) 586
TaqII GACCGA 1 cut(s) 606
TasI AATT 9 cut(s) 14, 109, 220, 305, 393, 547, 834, 1203, 1226
TfiI GAWTC 6 cut(s) 85, 245, 403, 905, 1130, 1291
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 6 cut(s) 5, 28, 462, 716, 741, 758
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 6 cut(s) 401, 444, 786, 1188, 1208, 1329
Tth111I GACNNNGTC 1 cut(s) 1238
VpaK11BI GGWCC 1 cut(s) 1058
XapI RAATTY 5 cut(s) 14, 109, 547, 834, 1226
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 1 cut(s) 299
ZraI GACGTC 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.