pycom16g04870

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008523
Physical Location & Seq
Reverse (-)
3123858 .. 3126513
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1173 bp
ATGAGTGTCAGGGAAAGAGAATCCACAACTCACATTACATATTTGTTTTTATCTTGTCTACTTGAATCTTTTCAAAATGTCCTCAACCCGACGTCGTTTAAGGCCATCTCTTGCTTCATTGCCAGTCGCGGCAAAACAGTAAACAAAAGCATCTTTCCCCTCCGCAACGCTGCTAGCTGCACAGCCACAGAAACGACAAAGGAGATGGTGACGCTGATTGTGGCGACGACGACCGACCCGGCCTCCATCAACCCGGCCAATGCCCTCCTAGCCATGCCTGGGTGGGACCCCTCCCCTTCCTGTTTACAGGACATGAAGGGGTTTGCTAATGGAAGAGGAGGAGTGAGGGTTTTGCTGCACAGCAGGGGAATTGTGGAAGAAGACGACTTGGACAGGCGGTGGGAGGAGGCCACCGGTGAGCGAGTCGACGAGGTGATATTCCTGAGCAAACACACCGCCGTCTCCAACCGGCCGGCACTGACCGTACACCCGATCGGAATTCCTCATCTGCGTGAAGGCGAGGCTGGGCCGCAAGGCGGGAAGCCGGGTTGGGCTGCGCCGCCGAGCCCCCGCATTGGACCCTGGCTCAGGCACCTGAAGAAGCTTGCCCAAGCCCACAACTTGGTTCCTGAGTTCGAGATTACATTGGAGGGTACGCATCATGGACCGGTAACGCATACGCCCACTATGTTCTTAGAGATTGGCAGTACAGATGAGTACTGGAAGAGGCAGGATGCAGCACAAGTTATTGCTCTATTGGTTTGGGAAGGACTTGGGCTTGGAGGAGGAGCTGCTGTTGGAAACTGGAACGGGGAGAAGGATAAGAATAAAGTTCTTCTTGGATTAGGTGGTGGACATTATGCTCCTCGGCATATGGATATAGTCCTGAAAGACGGGGTGTGGGTAGGCCATCTACTTTCGGGTTATTCCTTGCCGATGGAAGATCCAAATCAGTCCAAAGCAGGAACAAATGCAAAAGATACGAAAGACATTGGTGGAACTTGGAAACACTCAATCAAAGCAGCATTTGAGGCTACCCAGTCGGCTTTCCCTAGTGGGGAAGTTATAGCTCATCTTGATCAAACGAGTTTCAAAAGTTGGCAAAAGAACGCTATAACGGCTTTCTTAGGCGAGCAGAACATCAAAATTGGCAAACCCAACGATTTCTACTAG

Protein Analysis

391

Amino Acids

42.44

Weight (kDa)

6.74

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 139 - 383 1.4e-56 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 591
AccB7I CCANNNNNTGG 1 cut(s) 622
AccI GTMKAC 2 cut(s) 58, 426
AccII CGCG 1 cut(s) 129
AciI CCGC 8 cut(s) 129, 163, 397, 456, 530, 537, 560, 571
AclWI GGATC 1 cut(s) 938
AcoI YGGCCR 2 cut(s) 255, 470
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 617
AcyI GRCGYC 1 cut(s) 92
AfaI GTAC 4 cut(s) 486, 655, 709, 719
AfiI CCNNNNNNNGG 6 cut(s) 279, 536, 575, 588, 622, 1057
AgeI ACCGGT 2 cut(s) 413, 667
AgsI TTSAA 3 cut(s) 65, 74, 1093
AjnI CCWGG 2 cut(s) 277, 581
AluBI AGCT 4 cut(s) 177, 604, 791, 1070
AluI AGCT 4 cut(s) 177, 604, 791, 1070
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 1 cut(s) 938
AoxI GGCC 7 cut(s) 102, 240, 255, 408, 470, 527, 907
ApeKI GCWGC 7 cut(s) 170, 177, 355, 554, 737, 791, 1022
ApoI RAATTY 1 cut(s) 498
AsiGI ACCGGT 2 cut(s) 413, 667
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 4 cut(s) 286, 527, 578, 665
AsuC2I CCSGG 3 cut(s) 239, 254, 546
AsuHPI GGTGA 3 cut(s) 220, 428, 445
AsuNHI GCTAGC 1 cut(s) 173
AvaII GGWCC 3 cut(s) 286, 578, 665
BanI GGYRCC 1 cut(s) 591
BanII GRGCYC 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 7 cut(s) 157, 164, 342, 541, 749, 778, 1034
BccI CCATC 5 cut(s) 113, 199, 254, 918, 931
BceAI ACGGC 2 cut(s) 443, 1134
BciT130I CCWGG 2 cut(s) 279, 583
BclI TGATCA 1 cut(s) 1078
BcnI CCSGG 3 cut(s) 239, 254, 546
BcoDI GTCTC 1 cut(s) 466
BfaI CTAG 4 cut(s) 174, 269, 1053, 1171
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 5 cut(s) 239, 254, 279, 546, 583
Bme18I GGWCC 3 cut(s) 286, 578, 665
BmgT120I GGNCC 4 cut(s) 286, 527, 578, 665
BmiI GGNNCC 5 cut(s) 287, 288, 580, 593, 627
BmrFI CCNGG 5 cut(s) 239, 254, 279, 546, 583
BmrI ACTGGG 1 cut(s) 1033
BmsI GCATC 3 cut(s) 159, 667, 724
BmtI GCTAGC 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 1033
BpiI GAAGAC 1 cut(s) 387
Bpu10I CCTNAGC 2 cut(s) 443, 587
BpuMI CCSGG 3 cut(s) 239, 254, 546
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 278, 581, 866
BsaWI WCCGGW 2 cut(s) 413, 667
BsaXI ACNNNNNCTCC 6 cut(s) 227, 257, 333, 363, 780, 810
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 536, 575, 588, 622, 1057
Bse118I RCCGGY 4 cut(s) 413, 468, 472, 667
Bse1I ACTGG 4 cut(s) 123, 725, 809, 1039
Bse3DI GCAATG 1 cut(s) 117
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 2 cut(s) 279, 583
BseDI CCNNGG 3 cut(s) 278, 581, 866
BseGI GGATG 1 cut(s) 739
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 6 cut(s) 279, 536, 575, 588, 622, 1057
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 3 cut(s) 434, 601, 621
BseNI ACTGG 4 cut(s) 123, 725, 809, 1039
BseRI GAGGAG 6 cut(s) 351, 354, 419, 798, 801, 855
BseX3I CGGCCG 1 cut(s) 470
BseXI GCAGC 7 cut(s) 157, 164, 342, 541, 749, 778, 1034
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 2 cut(s) 163, 341
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 3 cut(s) 234, 473, 495
BshFI GGCC 7 cut(s) 104, 242, 257, 410, 472, 529, 909
BshNI GGYRCC 1 cut(s) 591
BshTI ACCGGT 2 cut(s) 413, 667
BsiEI CGRYCG 3 cut(s) 234, 473, 495
BsiSI CCGG 7 cut(s) 239, 254, 414, 469, 473, 545, 668
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 6 cut(s) 279, 536, 575, 588, 622, 1057
BsmAI GTCTC 1 cut(s) 466
BsmBI CGTCTC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 7 cut(s) 104, 242, 257, 410, 472, 529, 909
Bsp1286I GDGCHC 1 cut(s) 569
Bsp143I GATC 3 cut(s) 492, 943, 1078
BspACI CCGC 8 cut(s) 129, 163, 397, 456, 530, 537, 560, 571
BspANI GGCC 7 cut(s) 104, 242, 257, 410, 472, 529, 909
BspCNI CTCAG 3 cut(s) 435, 600, 622
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 5 cut(s) 287, 288, 580, 593, 627
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 117
BsrFI RCCGGY 4 cut(s) 413, 468, 472, 667
BsrI ACTGG 4 cut(s) 123, 725, 809, 1039
BssAI RCCGGY 4 cut(s) 413, 468, 472, 667
BssECI CCNNGG 3 cut(s) 278, 581, 866
BssMI GATC 3 cut(s) 492, 943, 1078
BssNI GRCGYC 1 cut(s) 92
Bst2UI CCWGG 2 cut(s) 279, 583
Bst4CI ACNGT 2 cut(s) 139, 484
Bst6I CTCTTC 2 cut(s) 328, 719
BstACI GRCGYC 1 cut(s) 92
BstC8I GCNNGC 4 cut(s) 175, 474, 606, 1133
BstDEI CTNAG 5 cut(s) 443, 587, 630, 694, 1126
BstENI CCTNNNNNAGG 1 cut(s) 586
BstF5I GGATG 1 cut(s) 739
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 3 cut(s) 495, 946, 1081
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 3 cut(s) 492, 943, 1078
BstMCI CGRYCG 3 cut(s) 234, 473, 495
BstMWI GCNNNNNNNGC 3 cut(s) 269, 1031, 1118
BstNI CCWGG 2 cut(s) 279, 583
BstSCI CCNGG 5 cut(s) 237, 252, 277, 544, 581
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 7 cut(s) 157, 164, 342, 541, 749, 778, 1034
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
BstZI CGGCCG 1 cut(s) 470
BsuRI GGCC 7 cut(s) 104, 242, 257, 410, 472, 529, 909
BtsCI GGATG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 476
Cac8I GCNNGC 4 cut(s) 175, 474, 606, 1133
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 4 cut(s) 413, 468, 472, 667
Cfr13I GGNCC 4 cut(s) 286, 527, 578, 665
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 485, 654, 708, 718
CspAI ACCGGT 2 cut(s) 413, 667
CviAII CATG 3 cut(s) 274, 313, 662
CviQI GTAC 4 cut(s) 485, 654, 708, 718
DdeI CTNAG 5 cut(s) 443, 587, 630, 694, 1126
DpnI GATC 3 cut(s) 494, 945, 1080
DpnII GATC 3 cut(s) 492, 943, 1078
EaeI YGGCCR 2 cut(s) 255, 470
EagI CGGCCG 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 328, 719
EarI CTCTTC 2 cut(s) 328, 719
EclXI CGGCCG 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 3 cut(s) 286, 578, 665
Eco52I CGGCCG 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 617
EcoNI CCTNNNNNAGG 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 277, 581
EcoT38I GRGCYC 1 cut(s) 569
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 3 cut(s) 277, 316, 665
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 1 cut(s) 299
FatI CATG 3 cut(s) 273, 312, 661
FauI CCCGC 2 cut(s) 530, 578
FauNDI CATATG 1 cut(s) 873
FbaI TGATCA 1 cut(s) 1078
FblI GTMKAC 2 cut(s) 58, 426
FokI GGATG 1 cut(s) 746
FriOI GRGCYC 1 cut(s) 569
FspBI CTAG 4 cut(s) 174, 269, 1053, 1171
GlaI GCGC 1 cut(s) 558
GsaI CCCAGC 1 cut(s) 528
HaeIII GGCC 7 cut(s) 104, 242, 257, 410, 472, 529, 909
HapII CCGG 7 cut(s) 239, 254, 414, 469, 473, 545, 668
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 92
Hin1II CATG 3 cut(s) 277, 316, 665
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 3 cut(s) 20, 65, 423
HpaII CCGG 7 cut(s) 239, 254, 414, 469, 473, 545, 668
HphI GGTGA 3 cut(s) 220, 428, 445
Hpy166II GTNNAC 6 cut(s) 59, 142, 305, 427, 487, 854
Hpy188I TCNGA 1 cut(s) 497
Hpy188III TCNNGA 5 cut(s) 442, 629, 637, 886, 1076
Hpy8I GTNNAC 6 cut(s) 59, 142, 305, 427, 487, 854
Hpy99I CGWCG 5 cut(s) 94, 97, 229, 232, 431
HpyAV CCTTC 5 cut(s) 306, 310, 509, 761, 811
HpyCH4III ACNGT 2 cut(s) 139, 484
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 180, 358, 737, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 1031, 1118
HpyF3I CTNAG 5 cut(s) 443, 587, 630, 694, 1126
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 92
Hsp92II CATG 3 cut(s) 277, 316, 665
HspAI GCGC 1 cut(s) 557
KflI GGGWCCC 1 cut(s) 286
KroI GCCGGC 1 cut(s) 472
KroNI GCCGGC 1 cut(s) 474
Ksp22I TGATCA 1 cut(s) 1078
Kzo9I GATC 3 cut(s) 492, 943, 1078
LmnI GCTCC 2 cut(s) 788, 868
Lsp1109I GCAGC 7 cut(s) 157, 164, 342, 541, 749, 778, 1034
LweI GCATC 3 cut(s) 159, 667, 724
MaeI CTAG 4 cut(s) 174, 269, 1053, 1171
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 2 cut(s) 208, 670
MalI GATC 3 cut(s) 494, 945, 1080
MboI GATC 3 cut(s) 492, 943, 1078
MboII GAAGA 7 cut(s) 345, 389, 392, 610, 736, 827, 953
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 1 cut(s) 569
MluCI AATT 3 cut(s) 369, 498, 1146
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 2 cut(s) 489, 778
MroNI GCCGGC 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 7 cut(s) 239, 254, 414, 469, 473, 545, 668
MspR9I CCNGG 5 cut(s) 239, 254, 279, 546, 583
MvaI CCWGG 2 cut(s) 279, 583
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 269, 1031, 1118
NaeI GCCGGC 1 cut(s) 474
NciI CCSGG 3 cut(s) 239, 254, 546
NdeI CATATG 1 cut(s) 873
NdeII GATC 3 cut(s) 492, 943, 1078
NgoMIV GCCGGC 1 cut(s) 472
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 277, 316, 665
NlaIV GGNNCC 5 cut(s) 287, 288, 580, 593, 627
NmeAIII GCCGAG 2 cut(s) 588, 847
NmuCI GTSAC 1 cut(s) 208
PdiI GCCGGC 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 20, 65
PflMI CCANNNNNTGG 1 cut(s) 622
PinAI ACCGGT 2 cut(s) 413, 667
Ple19I CGATCG 1 cut(s) 495
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 286
Psp6I CCWGG 2 cut(s) 277, 581
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 2 cut(s) 277, 581
PspN4I GGNNCC 5 cut(s) 287, 288, 580, 593, 627
PspPI GGNCC 4 cut(s) 286, 527, 578, 665
PspPPI RGGWCCY 1 cut(s) 286
PsuI RGATCY 1 cut(s) 943
PvuI CGATCG 1 cut(s) 495
RsaI GTAC 4 cut(s) 486, 655, 709, 719
RsaNI GTAC 4 cut(s) 485, 654, 708, 718
RseI CAYNNNNRTG 1 cut(s) 510
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 99
Sau3AI GATC 3 cut(s) 492, 943, 1078
Sau96I GGNCC 4 cut(s) 286, 527, 578, 665
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 5 cut(s) 239, 254, 279, 546, 583
SduI GDGCHC 1 cut(s) 569
SetI ASST 8 cut(s) 95, 179, 435, 597, 606, 793, 850, 1072
SfaNI GCATC 3 cut(s) 159, 667, 724
SgrAI CRCCGGYG 1 cut(s) 413
SinI GGWCC 3 cut(s) 286, 578, 665
SmiMI CAYNNNNRTG 1 cut(s) 510
Sse9I AATT 3 cut(s) 369, 498, 1146
SsiI CCGC 8 cut(s) 129, 163, 397, 456, 530, 537, 560, 571
SspMI CTAG 4 cut(s) 174, 269, 1053, 1171
StyD4I CCNGG 5 cut(s) 237, 252, 277, 544, 581
TaaI ACNGT 2 cut(s) 139, 484
TaiI ACGT 1 cut(s) 95
TaqI TCGA 2 cut(s) 426, 636
TaqII GACCGA 1 cut(s) 248
TasI AATT 3 cut(s) 369, 498, 1146
TatI WGTACW 2 cut(s) 707, 717
TauI GCSGC 3 cut(s) 132, 532, 562
TfiI GAWTC 2 cut(s) 20, 65
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 483
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 7 cut(s) 170, 177, 355, 554, 737, 791, 1022
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 106, 329
TspRI CASTG 1 cut(s) 483
Van91I CCANNNNNTGG 1 cut(s) 622
VpaK11BI GGWCC 3 cut(s) 286, 578, 665
XagI CCTNNNNNAGG 1 cut(s) 586
XapI RAATTY 1 cut(s) 498
XmiI GTMKAC 2 cut(s) 58, 426
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 4 cut(s) 174, 269, 1053, 1171
ZraI GACGTC 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.