pycom16g07460

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005814
Physical Location & Seq
Reverse (-)
4875835 .. 4876419
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGATCCAACGGACCAAAATGTATCACACGGCTGTGAAACATGGAATTGAGACGAGATCAGCTTCTGGTCAGTGCGGAACAATGGTGTTTGCAAATACTGTTGAGGCTGTGGAATCGGTAGCAAAGATATTGAAGAGAGCTGGAATGGAATGTTACCGATACCATAAAGATTGCTCCTTGGAAGACCGTGCAAAGACATTGGTTGATTTTCAAGAGAAAGGTGGCATTTTTGCATGCACTGATTCTGCCGCACGTGGCGTTGACATTCCAAATGTATCACACGTTATCCAGGCGGATTTTGCTACTTCTGCCGTGGACTTTATACACAGGGTTGGTCGCACAGCCAGAGCTGGTCAGTATGGACTTGTGACTAGCATGGGAACTTGA

Protein Analysis

129

Amino Acids

13.95

Weight (kDa)

8.75

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 27 - 118 7.2e-22 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 75, 249, 293
AcvI CACGTG 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 280
AgsI TTSAA 2 cut(s) 133, 212
AjnI CCWGG 1 cut(s) 288
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 3 cut(s) 62, 140, 350
AluI AGCT 3 cut(s) 62, 140, 350
Alw26I GTCTC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 12
AvaII GGWCC 1 cut(s) 12
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 189
BceAI ACGGC 2 cut(s) 45, 296
BciT130I CCWGG 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 44
BfaI CTAG 1 cut(s) 372
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 177, 312
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 177, 312
BsmAI GTCTC 1 cut(s) 44
BsmBI CGTCTC 1 cut(s) 44
Bsp143I GATC 2 cut(s) 3, 56
BspACI CCGC 3 cut(s) 75, 249, 293
BssECI CCNNGG 2 cut(s) 177, 312
BssMI GATC 2 cut(s) 3, 56
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 100, 188
Bst6I CTCTTC 1 cut(s) 128
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 235
BstDSI CCRYGG 1 cut(s) 312
BstKTI GATC 2 cut(s) 6, 59
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 2 cut(s) 3, 56
BstMWI GCNNNNNNNGC 2 cut(s) 299, 308
BstNI CCWGG 1 cut(s) 290
BstNSI RCATGY 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 77, 237
Cac8I GCNNGC 1 cut(s) 235
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 12
CviAII CATG 3 cut(s) 41, 234, 376
CviJI RGCY 6 cut(s) 32, 62, 107, 140, 344, 350
CviKI_1 RGCY 6 cut(s) 32, 62, 107, 140, 344, 350
DpnI GATC 2 cut(s) 5, 58
DpnII GATC 2 cut(s) 3, 56
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 12
Eco72I CACGTG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 3 cut(s) 44, 237, 379
FaiI YATR 6 cut(s) 42, 165, 235, 323, 360, 377
FatI CATG 3 cut(s) 40, 233, 375
Fnu4HI GCNGC 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 1 cut(s) 372
GluI GCNGC 1 cut(s) 249
Hin1II CATG 3 cut(s) 44, 237, 379
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 2 cut(s) 113, 242
Hpy166II GTNNAC 2 cut(s) 262, 316
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 262, 316
HpyCH4III ACNGT 2 cut(s) 100, 188
HpyCH4IV ACGT 2 cut(s) 253, 282
HpyCH4V TGCA 4 cut(s) 92, 191, 233, 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 308
HpySE526I ACGT 2 cut(s) 253, 282
Hsp92II CATG 3 cut(s) 44, 237, 379
Kzo9I GATC 2 cut(s) 3, 56
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 7 cut(s) 51, 126, 275, 302, 313, 336, 358
MaeI CTAG 1 cut(s) 372
MaeII ACGT 2 cut(s) 253, 282
MaeIII GTNAC 2 cut(s) 152, 367
MalI GATC 2 cut(s) 5, 58
MboI GATC 2 cut(s) 3, 56
MboII GAAGA 2 cut(s) 145, 194
MluCI AATT 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 1 cut(s) 97
MslI CAYNNNNRTG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 299, 308
NdeII GATC 2 cut(s) 3, 56
NlaIII CATG 3 cut(s) 44, 237, 379
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 1 cut(s) 237
OliI CACNNNNGTG 1 cut(s) 32
PaeI GCATGC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 113, 242
PkrI GCNGC 1 cut(s) 250
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 288
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 288
PspPI GGNCC 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 32
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 2 cut(s) 3, 56
Sau96I GGNCC 1 cut(s) 12
ScrFI CCNGG 1 cut(s) 290
SetI ASST 6 cut(s) 64, 142, 223, 256, 285, 352
SinI GGWCC 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 32
SphI GCATGC 1 cut(s) 237
Sse9I AATT 1 cut(s) 45
SsiI CCGC 3 cut(s) 75, 249, 293
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 2 cut(s) 100, 188
TaiI ACGT 2 cut(s) 256, 285
TasI AATT 1 cut(s) 45
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 2 cut(s) 113, 242
TscAI CASTG 2 cut(s) 77, 244
TseFI GTSAC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 367
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 77, 244
VpaK11BI GGWCC 1 cut(s) 12
XceI RCATGY 1 cut(s) 237
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.