Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0005814

Physical Location & Seq

Genomic Coordinates Reverse (-)

52624076 .. 52633075

Gene Structure 9000 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0354100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3633 bp

ATGCCCTCTTCACCATCTCCAATTCTTCTTCTTCTCCATCCCTCACCTCCTTCTCCACAATCACAATTCCCTTGGCGTCCCAAAGCCTCTGCCCTCAGACCCTCACCACTACTCGTCGCCATGAAGGACCGGCCTCCTTCTTCGTACGGCGCCGTTTACGTCCCTCCCCACCACCGCCTCCGCTCGGTCATCACCAGCCCTAATTACACCTCCGCCGCCTCAATTGCCTCCAAGAAGACTACTGCTGCTCCTCTCAGCAAAGCTCGCACCAATGGTGCTCGGACCTACTACCAGACTCAGCAGCACGAAGTTCAGGAACAGCTCCACAAGCCGAAGTTGCAGCACGATTCTGCTTACGACGGCGGCGGCGTCTCCGATGATGAAGGCTCCGATCGCGATTATAACATGTCGTCGAATCCGGGTGCTTCAATCTCTGATAACCTTGATGAGTGGACACGGAAGTTAACCATGCTGTTACGTGACAACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGAGGACATAGCCGCTTTGGCAAGCAGAATGGGACTTTACAGCCATCTGTATGCAAAAGTTGCTGTATTCAGTAAGGTCCCGCTGCCAAACTACAGATTTGATCTGGATGACCGGCGACCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCGTACCTCGGAGACTTCCTTTCTCAGAAGTCTAGGACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCTGAACCAGTGGCACCGAATAATGCTGCGATGGAAAAAGTTCTTTGGAGGAGAAGCTTGCAGCTGCGGGATAAGCAACACGCTTGGCAGGAATCTCGTGAAGGCAGAAAAGTTATGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAAGGATGCTTTGTTGACAGCAATTTCACGAAATCAGGTTGTTATCATATCAGGGCAAACAGGCTGTGGAAAGACCACTCAAATTCCACAATTCATTTTAGAATCTGAGACAGAAGCTGCCCGTGGAGCTGTATGTAGTATTATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCAGAAAGAGTTGCTTCAGAGAGAGGGGAGAAGTTGGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGCATGAAAGGGAGAGACACACGCCTTCTCTTCTGCACCACTGGCATTTTGTTGAGAAGATTGCTACTTGATGGAAATTTGAAAGGTGTAACTCATGTCATTGTGGATGAGATTCACGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTTCTGGAGGATGTTCTGGAAAGTACCGGTTGCAGGTTAACTCCATACAATCAGATTGATGATTATGGACAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAAGCAGCTGATTTTAAGGGTTACAGTCCCCAGACTCGGGAATCTCTAGCCTGTTGGAATCCTGATTGTATTGGTTTTAATCTCATAGAGTATCTGTTATGCAATATCTGTGAGAATGAAAGGCCTGGTGCCATTCTAGTATTTATGACTGGGTGGGATGACATCAATTCTCTAAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCAAGTCTTGTTGTTAGCATGCCATGGTTCCATGGCCAGTTCCGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGACGTTGTTTTTGTTATTGATTGTGGAAAGGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCTGGGCGCGTTCAACCTGGGGAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCATTTGCAGAATATCAATTGCCAGAAATCTTGAGGACACCTTTGCAGTCTCTTTGTCTGCAAATCAAAAGTTTAAAACTTGGAAGTATCTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTCGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAACGAGAATCTGACTATTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTCGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGAAAGATCTTGCGGAGGCTGCTAAATCTCAGTTTTCCCGAGACCACAGCGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCTCTCAGGAAGGAGTTCTACTCTTTGCTAAGGGATACTGATCTTATCGATGCCAACACAGCTACCTATAATGTATGGAGCTATGATGAGCATCTCGTCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATTTGCTCTGTTATGCACAATGAGAAGTCATATTCACTGAAAACTATGGAGGATGGTCAGGTGCTGCTATACTCGAACTCTGTCAATGCTCGGGAATCTAAAATTCCATATCCATGGCTAGTTTTTAATGAGAAGATAAAGGTGAACTCGGTTTTTCTACGGGATTCAACAGCTGTATCTGATTCAGTGCTTCTCCTATTTGGTGGAAGTTTTTCAAAAGGGGATATTGATGGACACTTGAAAATGTTGGGAGGATACTTGGAATTCTTCATGAAACCTGCTGTAGCAGAAATGTATCAATGTTTAAGGACAGAACTGGATGAGTTAATTCAAACCAAACTACGGAACCCAAGGATGGCCATCCACAAATATCATGAACTCCTTTCTGCAGTGCGGCAGCTGCTCTCGGAGGATCAATGTGAGGGTAGATTTGTATTTGGCCGCCAGGTTCGTACAACCTCAAAGCCATCTGTGGGGGCAGCTCAGCCAAGCTTGGTTTCAAGAACTGAAAGTGGACCTGGGGGTGATAATTCTAAGAGTCAGCTCCAGACATTGCTCACAAGGGCAGGATATGCTGCGCCCACCTACAAAACAATGCAACTAAAGAACCTCAAGTTCCAAGCTAGTGTGGAGTTCAATGGAATGCAAATTATGGGGCAGCCTTGTAATAACAAGAAGAGTGCAGAAAAAGATGCAGCAGCTGAGGCCATTCAGTGGCTTGTGAGTGGAACACAAATGGGCCATGACCACATCAATCACATGTCAATGATGTTGAAGAAAAGCAAAAAAGGATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009507 GO:0009536 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

1210

Amino Acids

135.8

Weight (kDa)

8.74

Isoelectric Point (pI)

49.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	328 - 480	1.2e-10	DEAD/DEAH box helicase
Helicase_C	PF00271	587 - 715	9e-13	Helicase conserved C-terminal domain
WHD_HA2	PF04408	779 - 807	6.1e-06	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	808 - 867	5e-16	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	934 - 1015	2.6e-20	Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD	PF26026	1024 - 1082	8.9e-24	C-terminal domain in RNA helicases
dsrm	PF00035	1124 - 1185	6.4e-09	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G59990 AT1G59990
fragaria_vesca	FvH4_4g35040 FvH4_4g35040 FvH4_4g35040 FvH4_4g35040
malus_domestica	MD00G1145400.v1.1 MD12G1027800.v1.1 MD13G1086600.v1.1 MD16G1086400.v1.1 MD16G1086500.v1.1
prunus_persica	Prupe.1G260000_v2.0.a1 Prupe.1G260000_v2.0.a1
pyrus_communis	pycom16g07460
rosa_chinensis	RchiOBHm_Chr4g0444581
rosa_laevigata	RLG00000005820
rosa_multiflora	Rmu_sc0003839.1_g000002 Rmu_sc0015511.1_g000001
rosa_roxburghii	Rroxscaffold_5G00385030
rosa_rugosa	Rorug04G0354000 Rorug04G0354000 Rorug04G0354100 Rorug04G0354100
rosa_samantha	Rh4AG414000 Rh4BG425800 Rh4CG440400 Rh4DG421400
rosa_wichuraiana	Rw4G035570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	402, 963
Acc36I	ACCTGC	2 cut(s)	1536, 3121
AccB1I	GGYRCC	5 cut(s)	149, 832, 1612, 1790, 2453
AccB7I	CCANNNNNTGG	2 cut(s)	2432, 3549
AccBSI	CCGCTC	1 cut(s)	183
AccII	CGCG	4 cut(s)	396, 1341, 2142, 2324
AclWI	GGATC	5 cut(s)	1386, 1865, 2468, 2507, 3255
AcoI	YGGCCR	4 cut(s)	1394, 1911, 3192, 3274
AcsI	RAATTY	6 cut(s)	1050, 1294, 2668, 2835, 2937, 3098
AcuI	CTGAAG	1 cut(s)	1149
AcyI	GRCGYC	3 cut(s)	76, 150, 369
AfaI	GTAC	6 cut(s)	146, 704, 796, 1117, 1537, 3289
AflIII	ACRYGT	2 cut(s)	405, 3594
AgeI	ACCGGT	1 cut(s)	1538
AjiI	CACGTC	1 cut(s)	527
AjnI	CCWGG	7 cut(s)	1483, 1786, 2093, 2149, 2830, 3279, 3352
AjuI	GAANNNNNNNTTGG	4 cut(s)	482, 514, 3316, 3348
AloI	GAACNNNNNNTCC	2 cut(s)	1191, 1223
Alw21I	GWGCWC	1 cut(s)	280
AlwI	GGATC	5 cut(s)	1386, 1865, 2468, 2507, 3255
AlwNI	CAGNNNCTG	3 cut(s)	1085, 2899, 3536
Ama87I	CYCGRG	3 cut(s)	1698, 2574, 2925
ApoI	RAATTY	6 cut(s)	1050, 1294, 2668, 2835, 2937, 3098
ArsI	GACNNNNNNTTYG	2 cut(s)	2003, 2035
AseI	ATTAAT	1 cut(s)	2007
AsiGI	ACCGGT	1 cut(s)	1538
Asp700I	GAANNNNTTC	5 cut(s)	747, 858, 1356, 2717, 3046
AspA2I	CCTAGG	1 cut(s)	1124
AspLEI	GCGC	3 cut(s)	152, 2142, 3415
AspS9I	GGNCC	6 cut(s)	127, 282, 610, 2361, 3350, 3574
AsuC2I	CCSGG	1 cut(s)	420
AsuII	TTCGAA	1 cut(s)	1504
AvaI	CYCGRG	3 cut(s)	1698, 2574, 2925
AvaII	GGWCC	4 cut(s)	127, 282, 610, 3350
AvrII	CCTAGG	1 cut(s)	1124
AxyI	CCTNAGG	1 cut(s)	1947
BaeI	ACNNNNGTAYC	2 cut(s)	2994, 3027
BalI	TGGCCA	2 cut(s)	1913, 3194
BanI	GGYRCC	5 cut(s)	149, 832, 1612, 1790, 2453
BanII	GRGCYC	3 cut(s)	810, 1468, 2302
BarI	GAAGNNNNNNTAC	2 cut(s)	2705, 2737
BauI	CACGAG	1 cut(s)	915
BbsI	GAAGAC	1 cut(s)	242
Bbv12I	GWGCWC	1 cut(s)	280
BbvCI	CCTCAGC	2 cut(s)	686, 3537
BceAI	ACGGC	3 cut(s)	137, 163, 376
BcgI	CGANNNNNNTGC	4 cut(s)	896, 930, 2576, 2610
BciT130I	CCWGG	7 cut(s)	1485, 1788, 2095, 2151, 2832, 3281, 3354
BciVI	GTATCC	3 cut(s)	2731, 2838, 3083
BcnI	CCSGG	1 cut(s)	420
BfmI	CTRYAG	4 cut(s)	625, 2303, 3117, 3222
BfoI	RGCGCY	1 cut(s)	153
BfuAI	ACCTGC	2 cut(s)	1536, 3121
BfuI	GTATCC	3 cut(s)	2731, 2838, 3083
BglI	GCCNNNNNGGC	1 cut(s)	551
BglII	AGATCT	1 cut(s)	2542
BlnI	CCTAGG	1 cut(s)	1124
BlpI	GCTNAGC	1 cut(s)	3318
Bme1390I	CCNGG	8 cut(s)	420, 1485, 1788, 2095, 2151, 2832, 3281, 3354
Bme18I	GGWCC	4 cut(s)	127, 282, 610, 3350
BmeT110I	CYCGRG	3 cut(s)	1698, 2574, 2925
BmgBI	CACGTC	1 cut(s)	527
BmgT120I	GGNCC	6 cut(s)	127, 282, 610, 2361, 3350, 3574
BmrFI	CCNGG	8 cut(s)	420, 1485, 1788, 2095, 2151, 2832, 3281, 3354
BmrI	ACTGGG	1 cut(s)	1821
BmsI	GCATC	8 cut(s)	964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
BmuI	ACTGGG	1 cut(s)	1821
BpiI	GAAGAC	1 cut(s)	242
BplI	GAGNNNNNCTC	4 cut(s)	1430, 1462, 2708, 2740
BpmI	CTGGAG	2 cut(s)	1538, 3365
Bpu10I	CCTNAGC	3 cut(s)	686, 2732, 3537
Bpu1102I	GCTNAGC	1 cut(s)	3318
Bpu14I	TTCGAA	1 cut(s)	1504
BpuEI	CTTGAG	3 cut(s)	1358, 2242, 3431
BpuMI	CCSGG	1 cut(s)	420
Bsa29I	ATCGAT	2 cut(s)	2687, 2751
BsaAI	YACGTR	1 cut(s)	479
BsaBI	GATNNNNATC	3 cut(s)	2742, 2793, 3194
BsaHI	GRCGYC	3 cut(s)	76, 150, 369
BsaI	GGTCTC	1 cut(s)	2571
BsaWI	WCCGGW	1 cut(s)	1538
BsaXI	ACNNNNNCTCC	2 cut(s)	232, 262
Bse118I	RCCGGY	4 cut(s)	129, 645, 1392, 1538
Bse1I	ACTGG	5 cut(s)	827, 1264, 1816, 1914, 3156
Bse21I	CCTNAGG	1 cut(s)	1947
Bse3DI	GCAATG	1 cut(s)	3386
Bse8I	GATNNNNATC	3 cut(s)	2742, 2793, 3194
BseBI	CCWGG	7 cut(s)	1485, 1788, 2095, 2151, 2832, 3281, 3354
BseCI	ATCGAT	2 cut(s)	2687, 2751
BseJI	GATNNNNATC	3 cut(s)	2742, 2793, 3194
BseMI	GCAATG	1 cut(s)	3386
BseNI	ACTGG	5 cut(s)	827, 1264, 1816, 1914, 3156
BseRI	GAGGAG	3 cut(s)	240, 703, 883
BseYI	CCCAGC	2 cut(s)	955, 2135
BsgI	GTGCAG	3 cut(s)	1237, 2826, 3537
Bsh1236I	CGCG	4 cut(s)	396, 1341, 2142, 2324
Bsh1285I	CGRYCG	1 cut(s)	394
BshNI	GGYRCC	5 cut(s)	149, 832, 1612, 1790, 2453
BshTI	ACCGGT	1 cut(s)	1538
BshVI	ATCGAT	2 cut(s)	2687, 2751
BsiEI	CGRYCG	1 cut(s)	394
BsiHKAI	GWGCWC	1 cut(s)	280
BsiHKCI	CYCGRG	3 cut(s)	1698, 2574, 2925
BsiSI	CCGG	5 cut(s)	130, 419, 646, 1393, 1539
BsiWI	CGTACG	1 cut(s)	144
BslFI	GGGAC	5 cut(s)	63, 146, 579, 596, 1674
BsmBI	CGTCTC	3 cut(s)	376, 1985, 2171
BsmFI	GGGAC	5 cut(s)	63, 146, 579, 596, 1674
BsmI	GAATGC	1 cut(s)	3483
Bso31I	GGTCTC	1 cut(s)	2571
BsoBI	CYCGRG	3 cut(s)	1698, 2574, 2925
Bsp119I	TTCGAA	1 cut(s)	1504
Bsp1286I	GDGCHC	4 cut(s)	280, 810, 1468, 2302
Bsp1407I	TGTACA	1 cut(s)	1115
Bsp1720I	GCTNAGC	1 cut(s)	3318
Bsp19I	CCATGG	3 cut(s)	1900, 1908, 2948
Bsp68I	TCGCGA	1 cut(s)	396
BspDI	ATCGAT	2 cut(s)	2687, 2751
BspFNI	CGCG	4 cut(s)	396, 1341, 2142, 2324
BspHI	TCATGA	2 cut(s)	3105, 3208
BspMAI	CTGCAG	1 cut(s)	3226
BspMI	ACCTGC	2 cut(s)	1536, 3121
BspPI	GGATC	5 cut(s)	1386, 1865, 2468, 2507, 3255
BspQI	GCTCTTC	1 cut(s)	2125
BspT104I	TTCGAA	1 cut(s)	1504
BspT107I	GGYRCC	5 cut(s)	149, 832, 1612, 1790, 2453
BspTNI	GGTCTC	1 cut(s)	2571
BsrBI	CCGCTC	1 cut(s)	183
BsrDI	GCAATG	1 cut(s)	3386
BsrFI	RCCGGY	4 cut(s)	129, 645, 1392, 1538
BsrGI	TGTACA	1 cut(s)	1115
BsrI	ACTGG	5 cut(s)	827, 1264, 1816, 1914, 3156
BssAI	RCCGGY	4 cut(s)	129, 645, 1392, 1538
BssNI	GRCGYC	3 cut(s)	76, 150, 369
BssSI	CACGAG	1 cut(s)	915
BssT1I	CCWWGG	8 cut(s)	71, 1124, 1900, 1908, 2103, 2364, 2948, 3185
Bst2BI	CACGAG	1 cut(s)	915
Bst2UI	CCWGG	7 cut(s)	1485, 1788, 2095, 2151, 2832, 3281, 3354
Bst4CI	ACNGT	2 cut(s)	1688, 2307
Bst6I	CTCTTC	5 cut(s)	13, 1253, 2119, 2125, 3506
BstACI	GRCGYC	3 cut(s)	76, 150, 369
BstAPI	GCANNNNNTGC	2 cut(s)	593, 3407
BstAUI	TGTACA	1 cut(s)	1115
BstBAI	YACGTR	1 cut(s)	479
BstBI	TTCGAA	1 cut(s)	1504
BstC8I	GCNNGC	8 cut(s)	265, 556, 878, 1394, 1851, 1897, 1974, 2607
BstDSI	CCRYGG	4 cut(s)	1090, 1900, 1908, 2948
BstEII	GGTNACC	1 cut(s)	664
BstENI	CCTNNNNNAGG	1 cut(s)	1863
BstFNI	CGCG	4 cut(s)	396, 1341, 2142, 2324
BstH2I	RGCGCY	1 cut(s)	153
BstHHI	GCGC	3 cut(s)	152, 2142, 3415
BstMCI	CGRYCG	1 cut(s)	394
BstNI	CCWGG	7 cut(s)	1485, 1788, 2095, 2151, 2832, 3281, 3354
BstNSI	RCATGY	4 cut(s)	409, 1853, 1899, 3598
BstPI	GGTNACC	1 cut(s)	664
BstSCI	CCNGG	8 cut(s)	418, 1483, 1786, 2093, 2149, 2830, 3279, 3352
BstSFI	CTRYAG	4 cut(s)	625, 2303, 3117, 3222
BstUI	CGCG	4 cut(s)	396, 1341, 2142, 2324
BstV2I	GAAGAC	1 cut(s)	242
BstX2I	RGATCY	3 cut(s)	1378, 2512, 2542
BstXI	CCANNNNNNTGG	1 cut(s)	2949
BstYI	RGATCY	3 cut(s)	1378, 2512, 2542
Bsu15I	ATCGAT	2 cut(s)	2687, 2751
Bsu36I	CCTNAGG	1 cut(s)	1947
BsuI	GTATCC	3 cut(s)	2731, 2838, 3083
BsuTUI	ATCGAT	2 cut(s)	2687, 2751
BtgI	CCRYGG	4 cut(s)	1090, 1900, 1908, 2948
BtgZI	GCGATG	1 cut(s)	863
BtrI	CACGTC	1 cut(s)	527
BtsI	GCAGTG	1 cut(s)	3231
BtsIMutI	CAGTG	7 cut(s)	834, 1257, 2063, 2870, 3027, 3231, 3554
BtuMI	TCGCGA	1 cut(s)	396
BveI	ACCTGC	2 cut(s)	1536, 3121
Cac8I	GCNNGC	8 cut(s)	265, 556, 878, 1394, 1851, 1897, 1974, 2607
CaiI	CAGNNNCTG	3 cut(s)	1085, 2899, 3536
CciI	TCATGA	2 cut(s)	3105, 3208
CfoI	GCGC	3 cut(s)	152, 2142, 3415
Cfr10I	RCCGGY	4 cut(s)	129, 645, 1392, 1538
Cfr13I	GGNCC	6 cut(s)	127, 282, 610, 2361, 3350, 3574
ClaI	ATCGAT	2 cut(s)	2687, 2751
CseI	GACGC	3 cut(s)	65, 358, 2406
Csp6I	GTAC	6 cut(s)	145, 703, 795, 1116, 1536, 3288
CspAI	ACCGGT	1 cut(s)	1538
CviQI	GTAC	6 cut(s)	145, 703, 795, 1116, 1536, 3288
DinI	GGCGCC	1 cut(s)	151
DraI	TTTAAA	2 cut(s)	2265, 2349
EaeI	YGGCCR	4 cut(s)	1394, 1911, 3192, 3274
Eam1104I	CTCTTC	5 cut(s)	13, 1253, 2119, 2125, 3506
EarI	CTCTTC	5 cut(s)	13, 1253, 2119, 2125, 3506
EciI	GGCGGA	1 cut(s)	202
Eco130I	CCWWGG	8 cut(s)	71, 1124, 1900, 1908, 2103, 2364, 2948, 3185
Eco147I	AGGCCT	1 cut(s)	1786
Eco24I	GRGCYC	3 cut(s)	810, 1468, 2302
Eco31I	GGTCTC	1 cut(s)	2571
Eco32I	GATATC	1 cut(s)	2100
Eco47I	GGWCC	4 cut(s)	127, 282, 610, 3350
Eco57I	CTGAAG	1 cut(s)	1149
Eco81I	CCTNAGG	1 cut(s)	1947
Eco88I	CYCGRG	3 cut(s)	1698, 2574, 2925
Eco91I	GGTNACC	1 cut(s)	664
EcoNI	CCTNNNNNAGG	1 cut(s)	1863
EcoO109I	RGGNCCY	2 cut(s)	610, 2361
EcoO65I	GGTNACC	1 cut(s)	664
EcoRI	GAATTC	1 cut(s)	3098
EcoRII	CCWGG	7 cut(s)	1483, 1786, 2093, 2149, 2830, 3279, 3352
EcoRV	GATATC	1 cut(s)	2100
EcoT14I	CCWWGG	8 cut(s)	71, 1124, 1900, 1908, 2103, 2364, 2948, 3185
EcoT22I	ATGCAT	1 cut(s)	2194
EcoT38I	GRGCYC	3 cut(s)	810, 1468, 2302
EgeI	GGCGCC	1 cut(s)	151
EheI	GGCGCC	1 cut(s)	151
ErhI	CCWWGG	8 cut(s)	71, 1124, 1900, 1908, 2103, 2364, 2948, 3185
Esp3I	CGTCTC	3 cut(s)	376, 1985, 2171
FalI	AAGNNNNNCTT	6 cut(s)	731, 763, 1147, 1179, 2429, 2461
FaqI	GGGAC	5 cut(s)	63, 146, 579, 596, 1674
FauI	CCCGC	2 cut(s)	621, 879
FauNDI	CATATG	1 cut(s)	2056
FriOI	GRGCYC	3 cut(s)	810, 1468, 2302
GlaI	GCGC	3 cut(s)	151, 2141, 3414
GsaI	CCCAGC	2 cut(s)	959, 2139
GsuI	CTGGAG	2 cut(s)	1538, 3365
HaeII	RGCGCY	1 cut(s)	153
HapII	CCGG	5 cut(s)	130, 419, 646, 1393, 1539
HgaI	GACGC	3 cut(s)	65, 358, 2406
HhaI	GCGC	3 cut(s)	152, 2142, 3415
Hin1I	GRCGYC	3 cut(s)	76, 150, 369
Hin6I	GCGC	3 cut(s)	150, 2140, 3413
HinP1I	GCGC	3 cut(s)	150, 2140, 3413
HincII	GTYRAC	3 cut(s)	465, 984, 1551
HindII	GTYRAC	3 cut(s)	465, 984, 1551
HindIII	AAGCTT	2 cut(s)	874, 3325
HpaI	GTTAAC	2 cut(s)	465, 1551
HpaII	CCGG	5 cut(s)	130, 419, 646, 1393, 1539
Hpy166II	GTNNAC	9 cut(s)	157, 453, 465, 984, 1118, 1551, 1607, 2980, 3350
Hpy8I	GTNNAC	9 cut(s)	157, 453, 465, 984, 1118, 1551, 1607, 2980, 3350
Hpy99I	CGWCG	3 cut(s)	119, 362, 415
HpyCH4III	ACNGT	2 cut(s)	1688, 2307
HpyCH4IV	ACGT	4 cut(s)	159, 478, 526, 2013
HpySE526I	ACGT	4 cut(s)	159, 478, 526, 2013
Hsp92I	GRCGYC	3 cut(s)	76, 150, 369
HspAI	GCGC	3 cut(s)	150, 2140, 3413
KasI	GGCGCC	1 cut(s)	149
KroI	GCCGGC	1 cut(s)	1392
KroNI	GCCGGC	1 cut(s)	1394
KspAI	GTTAAC	2 cut(s)	465, 1551
LguI	GCTCTTC	1 cut(s)	2125
LmnI	GCTCC	7 cut(s)	253, 327, 392, 1094, 2422, 2781, 3384
LweI	GCATC	8 cut(s)	964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
MaeII	ACGT	4 cut(s)	159, 478, 526, 2013
MaeIII	GTNAC	7 cut(s)	474, 479, 664, 1306, 1682, 2307, 2316
MbiI	CCGCTC	1 cut(s)	183
MfeI	CAATTG	2 cut(s)	222, 2207
MflI	RGATCY	3 cut(s)	1378, 2512, 2542
MhlI	GDGCHC	4 cut(s)	280, 810, 1468, 2302
MlsI	TGGCCA	2 cut(s)	1913, 3194
MluNI	TGGCCA	2 cut(s)	1913, 3194
Mly113I	GGCGCC	1 cut(s)	150
MlyI	GAGTC	3 cut(s)	289, 1690, 3382
MmeI	TCCRAC	2 cut(s)	1177, 1697
Mox20I	TGGCCA	2 cut(s)	1913, 3194
Mph1103I	ATGCAT	1 cut(s)	2194
MroNI	GCCGGC	1 cut(s)	1392
MroXI	GAANNNNTTC	5 cut(s)	747, 858, 1356, 2717, 3046
MscI	TGGCCA	2 cut(s)	1913, 3194
MslI	CAYNNNNRTG	3 cut(s)	582, 2947, 3599
Msp20I	TGGCCA	2 cut(s)	1913, 3194
MspA1I	CMGCKG	6 cut(s)	616, 883, 1670, 3008, 3235, 3536
MspI	CCGG	5 cut(s)	130, 419, 646, 1393, 1539
MspR9I	CCNGG	8 cut(s)	420, 1485, 1788, 2095, 2151, 2832, 3281, 3354
MunI	CAATTG	2 cut(s)	222, 2207
Mva1269I	GAATGC	1 cut(s)	3483
MvaI	CCWGG	7 cut(s)	1485, 1788, 2095, 2151, 2832, 3281, 3354
MvnI	CGCG	4 cut(s)	396, 1341, 2142, 2324
NaeI	GCCGGC	1 cut(s)	1394
NarI	GGCGCC	1 cut(s)	150
NciI	CCSGG	1 cut(s)	420
NcoI	CCATGG	3 cut(s)	1900, 1908, 2948
NdeI	CATATG	1 cut(s)	2056
NgoMIV	GCCGGC	1 cut(s)	1392
NmuCI	GTSAC	3 cut(s)	479, 664, 2307
NruI	TCGCGA	1 cut(s)	396
NsiI	ATGCAT	1 cut(s)	2194
NspI	RCATGY	4 cut(s)	409, 1853, 1899, 3598
NspV	TTCGAA	1 cut(s)	1504
PaeI	GCATGC	2 cut(s)	1853, 1899
PagI	TCATGA	2 cut(s)	3105, 3208
PceI	AGGCCT	1 cut(s)	1786
PciI	ACATGT	2 cut(s)	405, 3594
PciSI	GCTCTTC	1 cut(s)	2125
PcsI	WCGNNNNNNNCGW	1 cut(s)	366
PctI	GAATGC	1 cut(s)	3483
PdiI	GCCGGC	1 cut(s)	1394
PdmI	GAANNNNTTC	5 cut(s)	747, 858, 1356, 2717, 3046
Pfl23II	CGTACG	1 cut(s)	144
PflMI	CCANNNNNTGG	2 cut(s)	2432, 3549
PfoI	TCCNGGA	2 cut(s)	2093, 2830
PinAI	ACCGGT	1 cut(s)	1538
Ple19I	CGATCG	1 cut(s)	394
PleI	GAGTC	3 cut(s)	289, 1690, 3381
PluTI	GGCGCC	1 cut(s)	153
PpsI	GAGTC	3 cut(s)	289, 1690, 3381
Ppu21I	YACGTR	1 cut(s)	479
PpuMI	RGGWCCY	1 cut(s)	610
PscI	ACATGT	2 cut(s)	405, 3594
PshBI	ATTAAT	1 cut(s)	2007
PsiI	TTATAA	2 cut(s)	402, 963
Psp5II	RGGWCCY	1 cut(s)	610
Psp6I	CCWGG	7 cut(s)	1483, 1786, 2093, 2149, 2830, 3279, 3352
PspEI	GGTNACC	1 cut(s)	664
PspFI	CCCAGC	2 cut(s)	955, 2135
PspGI	CCWGG	7 cut(s)	1483, 1786, 2093, 2149, 2830, 3279, 3352
PspLI	CGTACG	1 cut(s)	144
PspPI	GGNCC	6 cut(s)	127, 282, 610, 2361, 3350, 3574
PspPPI	RGGWCCY	1 cut(s)	610
PstI	CTGCAG	1 cut(s)	3226
PstNI	CAGNNNCTG	3 cut(s)	1085, 2899, 3536
PsuI	RGATCY	3 cut(s)	1378, 2512, 2542
PvuI	CGATCG	1 cut(s)	394
PvuII	CAGCTG	5 cut(s)	883, 1670, 3008, 3235, 3536
RruI	TCGCGA	1 cut(s)	396
RsaI	GTAC	6 cut(s)	146, 704, 796, 1117, 1537, 3289
RsaNI	GTAC	6 cut(s)	145, 703, 795, 1116, 1536, 3288
RseI	CAYNNNNRTG	3 cut(s)	582, 2947, 3599
SapI	GCTCTTC	1 cut(s)	2125
Sau96I	GGNCC	6 cut(s)	127, 282, 610, 2361, 3350, 3574
SchI	GAGTC	3 cut(s)	289, 1690, 3382
ScrFI	CCNGG	8 cut(s)	420, 1485, 1788, 2095, 2151, 2832, 3281, 3354
SduI	GDGCHC	4 cut(s)	280, 810, 1468, 2302
SfaNI	GCATC	8 cut(s)	964, 1645, 2050, 2179, 2430, 2743, 2803, 3517
SfcI	CTRYAG	4 cut(s)	625, 2303, 3117, 3222
SfoI	GGCGCC	1 cut(s)	151
SfuI	TTCGAA	1 cut(s)	1504
SinI	GGWCC	4 cut(s)	127, 282, 610, 3350
SmiMI	CAYNNNNRTG	3 cut(s)	582, 2947, 3599
SmlI	CTYRAG	3 cut(s)	1373, 2221, 3446
SmoI	CTYRAG	3 cut(s)	1373, 2221, 3446
SphI	GCATGC	2 cut(s)	1853, 1899
SseBI	AGGCCT	1 cut(s)	1786
SspDI	GGCGCC	1 cut(s)	149
SspI	AATATT	2 cut(s)	1935, 1984
StuI	AGGCCT	1 cut(s)	1786
StyD4I	CCNGG	8 cut(s)	418, 1483, 1786, 2093, 2149, 2830, 3279, 3352
StyI	CCWWGG	8 cut(s)	71, 1124, 1900, 1908, 2103, 2364, 2948, 3185
TaaI	ACNGT	2 cut(s)	1688, 2307
TaiI	ACGT	4 cut(s)	162, 481, 529, 2016
TaqI	TCGA	6 cut(s)	413, 1504, 2472, 2687, 2751, 2909
TaqII	GACCGA	1 cut(s)	175
TatI	WGTACW	1 cut(s)	1115
TauI	GCSGC	6 cut(s)	218, 366, 369, 548, 3232, 3279
TscAI	CASTG	7 cut(s)	834, 1264, 2070, 2877, 3027, 3231, 3554
TseFI	GTSAC	3 cut(s)	479, 664, 2307
Tsp45I	GTSAC	3 cut(s)	479, 664, 2307
TspGWI	ACGGA	3 cut(s)	472, 2792, 3193
TspRI	CASTG	7 cut(s)	834, 1264, 2070, 2877, 3027, 3231, 3554
Van91I	CCANNNNNTGG	2 cut(s)	2432, 3549
VpaK11BI	GGWCC	4 cut(s)	127, 282, 610, 3350
VspI	ATTAAT	1 cut(s)	2007
XagI	CCTNNNNNAGG	1 cut(s)	1863
XapI	RAATTY	6 cut(s)	1050, 1294, 2668, 2835, 2937, 3098
XbaI	TCTAGA	1 cut(s)	767
XceI	RCATGY	4 cut(s)	409, 1853, 1899, 3598
XmaJI	CCTAGG	1 cut(s)	1124
XmnI	GAANNNNTTC	5 cut(s)	747, 858, 1356, 2717, 3046
Zsp2I	ATGCAT	1 cut(s)	2194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.