Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

5526451 .. 5535790

Gene Structure 9340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4215 bp

ATGAGCAATTCCGTAGGCCATAACTTGCTCAATCAGAGCTTGTTTCAAAGCAAGATAAATTCTTCTGGTATTCCTGCCAACACATTGTTTCAAGCTAAGAGCGTCCACCAAGTGACAGCTCAGGCGCGAAAATCACCCATATCCAAGAAATTCTGTGGGAACACTTTGAACGTTCAGAAACCGAAGTTAGCCATGGGGTCATGCCGCCCCATTACAGCTATTCCTCGTGGCGTATTAACCACTGATCCGCCTTCCAATCAGCTTGCCAGGAAATTCAATCTCGATGGGAATATTGAAATGCAGGTTTATGTGAATCCTTCCACACCGGGATCTGCCACTCAAGTAGATATCCGTGTAGCATATAGCGGTGACTCTTTGACTCTTCACTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCATCTCGTCACCCAGATGGAACCAAAGTATATAAGAACAAAGCTCTTAGAACACCTTTTGTGAATTCCGGCTCCAATTCCTTGCTTCAAATTGAGATTGATGATCCTACAATACAAGCAATCGAATTTCTTTTATTTGATGAAAGCCAGAACAGATGGTTTAAGAACAATGATGATAACTTTCATGTTAAGTTACCTGCAAAGGAGAAGTTGATATCCAATGCTTCAGTTCCTGAAGAGCTTGTACAAATTCAAGCATATTTGAGGTGGGAAAGAAAGGGTAAACAGATGTATACACCAGAGCAAGAAAAGGTGGAGTACGAAGCAGCCCGAACTGAGTTATTGGAGGAAGTAGCTAGGGGTACTTCCATACAAGAGCTCCAAGCAAGGTTGACTAAGAAAAATGATGGTGGTAAGATTGAAGAGCCATCTGGTTCTGATACAAAGGCTGCAAAAAAATCTGAAAGCAAAAAGGTTTTTAGAACCGAGAGAATTCAACGCAAGAGGAGGGACTTGATGCGGATTATCAACAAACATGCTACTAAACCTGTTGATGAAGCTAAATTTGTAGATGAAGAACATCCAGCCAAACCAAAACCCTTGACAGCAGTTGAGCTTTTCGCCAAGGAAAAGGAAGAACAGGATGGAGGTTCTATTCTGAGGAAAAACATCTTCAAGATCAATGATAAGGAACTTCTGGTACTTGTAACCAAGCCTGCTGGGAAGACAAAGGTTCATCTGGCAACAGATTTCAAAGAGCCACTTACCCTTCACTGGGCTTTATCTGCAAGCAAGGCTGGAGAGTGGTCGGAACCACCTGCAAATACACATCCCCAGGGTTCAGTTGCTCTAAATGGGGCTGTTGAAACACAATTTAGACCAAGTTCCACTGACTCTGCTTATGAGGTTCAATCTATAGAAATAGAAATTGAAGGAGAGAGTTTTAAAGGAATGCCGTTTGTCCTTAATGCTTCTGGGAACTGGATGAAGAATCAAGGCTCCGATTTCTATGTTGACTTTGGCGTCGAACGCAAGAAAGTTCAGAAGGATACTGGTGATGGCAAAGGTACTGCAAAGGCTTTGTTGGATAAAATAGCCGACCAGGAGAGTGAGGCACAGAAATCCTTTATGCATCGATTTAACATTGCAGCAGACTTGGTCAATCAAGCAAAAGATGCTGGTGAATTAGGTCTTGCAGGTATATTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTCATTTGGAATAAAAATTACAATGTGAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACGGACTTACTCCAGAATGTTTATGCAAATTATCCACAGTTTCGCGAACTTCTACGGATGATCTTGTCTACCGTTGGCCGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGAGAAAAAATAATTGCAAAGGTGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACAAGTCCAGATGATGTTGTTATTTGTCAGGCATTAATAGATTACATAAAAAATGACTTTGATATTGGTGTTTACTGGAAAACTTTGAATGAGAATGGAATCACAAAAGAACGTCTCCTGAGCTACGATCGTGCAATCCGTAATGAACCAAATTTCAGGAGAGATCAGAAAGATGGTCTTCTGCGTGATCTGGGGCACTACATGAGAACTTTGAAGGCTGTTCATTCAGGTGCAGATCTTGAGTCTGCCATTCAAAATTGTATGGGCTACAAAGATGAGGGTCAAGGATTCATGGTTGGAGTACAAATAAATCCTATATCCGGCTTGCCATCTGAAGTTCCAGACCTCCTGCGATTTGTACTAGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCGCGCCAGGCGCTTTGGCCATTGCTCTCTAAGCCTCATGATCGTCTGATGGATCTTTTATTTTTAGACATTGCCCTTGATTCTACTGTTAGGACCGCAATTGAGAGGGGATATGAAGAACTGAATAATGCTGGACCAGAGAAAATTATGTACTTCATCTCTTTAGTTCTAGAAAACCTGGCCCTATCATCAGACGATAACGAAGATCTCATTTACTGTTTGAAGGGATGGGATCAGGCTCAAAACATGTTAAAAAGTAAAAGCAATGACTGGGCATTATATTCAAAATCAATCCTAGACAGAACCCGCCTTGCACTTGCAAACAAGGCAGAATCGTATCACAGAATTTTACAGCCATCAGCAGAATACCTCGGAGCACAGCTTGGAGTGGACCAATGGGCTGTGAACATATTTACTGAAGAAATAATCCGTGCTGGATCAGCTGCATCTTTATCCTCACTTCTGAATCGGCTTGATCCGGTTCTTCGAAAGACTGCTAATATGGGCAGTTGGCAGGTTATCAGCCCAGTTGAAGTTGTTGGATATGTGGTCTATGTGGATGAATTGCTCACTGTTCAGAATAAAGTTTACAGCAAGCCCACAATTTTGGTGGCAAAAAGTGTAAAAGGTGAGGAAGAAATTCCAGATGGCACTGTTGCTGTTCTGACACCAGATATGCCAGACATCCTATCTCATGTCTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGCTTTGACCCCAATATTCTGGCTGACCTCCGAGCTAGTGAAGGGAAGTTATTACGTGTAAAGCCAACACCTGCTGATATAGTTTATAGTGAGGTCAATGAGGGTGAGCTAGATGCAGGTTCCACTAATTCAAAAGAAGACACTCCATCTCTGACATTGGTCAAGAAAAAATTTACTGGTAGATATGCCATATCATCTGATGAGTTCACAAGCGATATGGTTGGAGCTAAATCACGTAATATTGCTTATGTAAAAGGAAAACTTCCGTCTTGGGTTGGAATTCCTACATCGGTTGCCCTACCATTTGGATCATTTGAAAAGGTTCTTTCAGAAGATTCAAACAAGGCAGTGGCTGAAAAGTTGGAAACTCTAAAGAAAAAGTTGAAAGACGGAGATTTTGATTCGCTCAAGAAGATTCGGGAAGCAGTTTTACAGCTGGCAGCACCGCCCCAGTTGGTGCAAGAGCTGAAGATGACAATGAAGGGGTCAGGGATGCCTTGGCCTGGTGATGAAGGTGAACAACGGTGGGAACAAGCATGGACGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAACGAGAGAGCGTACTTCAGCACGAGAAAAGTGAAATTAGATCACGACTATCTTTGCATGGCTGTCCTTGTTCAGGAAATCATAAACGCTGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCATCAGAGATATATGCTGAGGTTGTTAAGGGGCTTGGAGAAACCCTAGTTGGAGCTTATCCTGGACGTGCTCTAAGTTTTATTAGCAAAAAGAATGATCTGGACTCTCCTCAGGTAATAGGATACCCTAGCAAACCCATTGGCCTCTTTATAAAACGGTCTATAATCTTTCGATCTGATTCCAATGGTGAAGATCTCGAAGGTTATGCTGGTGCAGGTCTTTACGACAGTGTGCCTATGGATGAGGAAGAGAAAGTCGTGGTTGACTACTCATCTGACCTGCTGATGGTTGACGGAAACTTCCGCAAGACAATCCTCTCTAGCATTGCTCGTGCAGGAAGCGCAATCGAGGAACTGTATGGATCTCCACAAGACATTGAAGGTGTAATCAGGGACGGTAAACTCTATGTTGTCCAGACAAGACCCCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1405

Amino Acids

156.91

Weight (kDa)

6.4

Isoelectric Point (pI)

38.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	89 - 207	1.2e-40	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	221 - 276	9.3e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	365 - 482	8.9e-30	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	948 - 1060	8.6e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1201 - 1403	6.7e-21	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3932
AarI	CACCTGC	2 cut(s)	1252, 3151
AasI	GACNNNNNNGTC	1 cut(s)	1448
Acc36I	ACCTGC	9 cut(s)	292, 631, 1252, 1613, 2839, 3151, 3179, 3986, 4068
AccI	GTMKAC	2 cut(s)	719, 1793
AccII	CGCG	3 cut(s)	127, 1770, 2338
AciI	CCGC	8 cut(s)	205, 248, 366, 946, 2430, 2641, 3518, 4084
AclI	AACGTT	1 cut(s)	171
AcoI	YGGCCR	2 cut(s)	1801, 2351
AcuI	CTGAAG	7 cut(s)	636, 681, 2274, 2772, 3560, 3646, 3756
AcvI	CACGTG	1 cut(s)	1697
AcyI	GRCGYC	1 cut(s)	1449
AdeI	CACNNNGTG	1 cut(s)	112
AfaI	GTAC	9 cut(s)	672, 746, 790, 1128, 1495, 2223, 2280, 2486, 3659
AfiI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
AflIII	ACRYGT	3 cut(s)	2287, 2580, 3127
AjiI	CACGTC	1 cut(s)	3848
AjnI	CCWGG	7 cut(s)	266, 1260, 1527, 2340, 2511, 3574, 3841
AjuI	GAANNNNNNNTTGG	2 cut(s)	3813, 3845
AleI	CACNNNNGTG	1 cut(s)	390
Alw21I	GWGCWC	3 cut(s)	807, 2713, 3853
Alw26I	GTCTC	3 cut(s)	2039, 3037, 3771
AlwNI	CAGNNNCTG	2 cut(s)	659, 3425
AoxI	GGCC	6 cut(s)	16, 1801, 2351, 2514, 3572, 3922
ApeKI	GCWGC	6 cut(s)	752, 875, 1574, 1660, 2777, 3512
ArsI	GACNNNNNNTTYG	6 cut(s)	1818, 1850, 3452, 3484, 3728, 3760
AscI	GGCGCGCC	1 cut(s)	2336
AseI	ATTAAT	1 cut(s)	1955
Asp700I	GAANNNNTTC	2 cut(s)	2973, 3393
AspLEI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
AspS9I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
AsuC2I	CCSGG	1 cut(s)	327
AsuII	TTCGAA	1 cut(s)	2821
AvaII	GGWCC	3 cut(s)	2427, 2468, 2725
AxyI	CCTNAGG	1 cut(s)	3891
BaeGI	GKGCMC	1 cut(s)	2118
BalI	TGGCCA	1 cut(s)	2353
BanII	GRGCYC	1 cut(s)	807
BarI	GAAGNNNNNNTAC	4 cut(s)	654, 686, 3486, 3518
BauI	CACGAG	3 cut(s)	225, 3667, 4110
BbrPI	CACGTG	1 cut(s)	1697
BbsI	GAAGAC	3 cut(s)	1157, 2090, 3216
Bbv12I	GWGCWC	3 cut(s)	807, 2713, 3853
BbvCI	CCTCAGC	1 cut(s)	3798
BbvI	GCAGC	6 cut(s)	764, 862, 1586, 1672, 2764, 3524
BceAI	ACGGC	3 cut(s)	1366, 1788, 3630
BcgI	CGANNNNNNTGC	2 cut(s)	3968, 4002
BciT130I	CCWGG	7 cut(s)	268, 1262, 1529, 2342, 2513, 3576, 3843
BciVI	GTATCC	2 cut(s)	1468, 3896
BcnI	CCSGG	1 cut(s)	327
BcoDI	GTCTC	3 cut(s)	2039, 3037, 3771
BfaI	CTAG	9 cut(s)	783, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
BfmI	CTRYAG	1 cut(s)	1341
BfoI	RGCGCY	1 cut(s)	2348
BfuAI	ACCTGC	9 cut(s)	292, 631, 1252, 1613, 2839, 3151, 3179, 3986, 4068
BfuI	GTATCC	2 cut(s)	1468, 3896
BglII	AGATCT	3 cut(s)	2155, 2539, 3973
BisI	GCNGC	7 cut(s)	205, 753, 876, 1575, 1661, 2778, 3513
BlsI	GCNGC	7 cut(s)	206, 754, 877, 1576, 1662, 2779, 3514
Bme1390I	CCNGG	8 cut(s)	268, 327, 1262, 1529, 2342, 2513, 3576, 3843
Bme18I	GGWCC	3 cut(s)	2427, 2468, 2725
BmgBI	CACGTC	1 cut(s)	3848
BmgT120I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
BmiI	GGNNCC	5 cut(s)	448, 499, 1239, 1426, 3193
BmrFI	CCNGG	8 cut(s)	268, 327, 1262, 1529, 2342, 2513, 3576, 3843
BmrI	ACTGGG	5 cut(s)	397, 1210, 2614, 2855, 3517
BmsI	GCATC	7 cut(s)	933, 1567, 1591, 1909, 2789, 3175, 3555
BmuI	ACTGGG	5 cut(s)	397, 1210, 2614, 2855, 3517
BoxI	GACNNNNGTC	1 cut(s)	3230
BpiI	GAAGAC	3 cut(s)	1157, 2090, 3216
BpmI	CTGGAG	2 cut(s)	1245, 1721
Bpu10I	CCTNAGC	3 cut(s)	120, 2039, 3798
Bpu14I	TTCGAA	1 cut(s)	2821
BpuEI	CTTGAG	4 cut(s)	324, 2180, 2351, 3464
BpuMI	CCSGG	1 cut(s)	327
Bsa29I	ATCGAT	1 cut(s)	1561
BsaAI	YACGTR	3 cut(s)	1697, 3128, 3308
BsaHI	GRCGYC	1 cut(s)	1449
BsaJI	CCNNGG	7 cut(s)	192, 1050, 1260, 1261, 1804, 2704, 3569
BsaWI	WCCGGW	1 cut(s)	2812
BsaXI	ACNNNNNCTCC	2 cut(s)	789, 819
Bsc4I	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
Bse1I	ACTGG	9 cut(s)	392, 1205, 1412, 1483, 2000, 2609, 2861, 3253, 3523
Bse21I	CCTNAGG	1 cut(s)	3891
Bse3DI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BseBI	CCWGG	7 cut(s)	268, 1262, 1529, 2342, 2513, 3576, 3843
BseCI	ATCGAT	1 cut(s)	1561
BseDI	CCNNGG	7 cut(s)	192, 1050, 1260, 1261, 1804, 2704, 3569
BseLI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
BseMI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BseMII	CTCAG	6 cut(s)	134, 753, 1076, 2030, 3789, 3905
BseNI	ACTGG	9 cut(s)	392, 1205, 1412, 1483, 2000, 2609, 2861, 3253, 3523
BsePI	GCGCGC	1 cut(s)	2336
BseRI	GAGGAG	3 cut(s)	946, 1824, 3879
BseSI	GKGCMC	1 cut(s)	2118
BseXI	GCAGC	6 cut(s)	764, 862, 1586, 1672, 2764, 3524
BseYI	CCCAGC	1 cut(s)	1145
BsgI	GTGCAG	3 cut(s)	2172, 4014, 4134
Bsh1236I	CGCG	3 cut(s)	127, 1770, 2338
Bsh1285I	CGRYCG	1 cut(s)	2050
BshFI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BshVI	ATCGAT	1 cut(s)	1561
BsiEI	CGRYCG	1 cut(s)	2050
BsiHKAI	GWGCWC	3 cut(s)	807, 2713, 3853
BsiSI	CCGG	4 cut(s)	326, 495, 2241, 2813
BslFI	GGGAC	3 cut(s)	950, 1839, 4187
BslI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
BsmAI	GTCTC	3 cut(s)	2039, 3037, 3771
BsmBI	CGTCTC	1 cut(s)	2039
BsmFI	GGGAC	3 cut(s)	950, 1839, 4187
BsmI	GAATGC	2 cut(s)	1383, 3743
BsnI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
Bsp119I	TTCGAA	1 cut(s)	2821
Bsp1286I	GDGCHC	4 cut(s)	807, 2118, 2713, 3853
Bsp1407I	TGTACA	1 cut(s)	670
Bsp19I	CCATGG	1 cut(s)	192
Bsp68I	TCGCGA	1 cut(s)	1770
BspACI	CCGC	8 cut(s)	205, 248, 366, 946, 2430, 2641, 3518, 4084
BspANI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BspCNI	CTCAG	6 cut(s)	133, 754, 1077, 2031, 3790, 3904
BspDI	ATCGAT	1 cut(s)	1561
BspFNI	CGCG	3 cut(s)	127, 1770, 2338
BspHI	TCATGA	1 cut(s)	2371
BspLI	GGNNCC	5 cut(s)	448, 499, 1239, 1426, 3193
BspMI	ACCTGC	9 cut(s)	292, 631, 1252, 1613, 2839, 3151, 3179, 3986, 4068
BspQI	GCTCTTC	2 cut(s)	657, 843
BspT104I	TTCGAA	1 cut(s)	2821
BsrDI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BsrGI	TGTACA	1 cut(s)	670
BsrI	ACTGG	9 cut(s)	392, 1205, 1412, 1483, 2000, 2609, 2861, 3253, 3523
BssECI	CCNNGG	7 cut(s)	192, 1050, 1260, 1261, 1804, 2704, 3569
BssHII	GCGCGC	1 cut(s)	2336
BssNAI	GTATAC	1 cut(s)	720
BssNI	GRCGYC	1 cut(s)	1449
BssSI	CACGAG	3 cut(s)	225, 3667, 4110
BssT1I	CCWWGG	3 cut(s)	192, 1050, 3569
Bst1107I	GTATAC	1 cut(s)	720
Bst2BI	CACGAG	3 cut(s)	225, 3667, 4110
Bst2UI	CCWGG	7 cut(s)	268, 1262, 1529, 2342, 2513, 3576, 3843
Bst6I	CTCTTC	4 cut(s)	387, 657, 843, 4023
BstACI	GRCGYC	1 cut(s)	1449
BstAPI	GCANNNNNTGC	1 cut(s)	1601
BstAUI	TGTACA	1 cut(s)	670
BstBAI	YACGTR	3 cut(s)	1697, 3128, 3308
BstBI	TTCGAA	1 cut(s)	2821
BstC8I	GCNNGC	7 cut(s)	264, 1143, 1216, 2246, 2338, 2930, 3510
BstDSI	CCRYGG	2 cut(s)	192, 1804
BstENI	CCTNNNNNAGG	2 cut(s)	2318, 3716
BstFNI	CGCG	3 cut(s)	127, 1770, 2338
BstH2I	RGCGCY	1 cut(s)	2348
BstHHI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
BstMAI	GTCTC	3 cut(s)	2039, 3037, 3771
BstMCI	CGRYCG	1 cut(s)	2050
BstNI	CCWGG	7 cut(s)	268, 1262, 1529, 2342, 2513, 3576, 3843
BstNSI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
BstPAI	GACNNNNGTC	1 cut(s)	3230
BstSCI	CCNGG	8 cut(s)	266, 325, 1260, 1527, 2340, 2511, 3574, 3841
BstSFI	CTRYAG	1 cut(s)	1341
BstSLI	GKGCMC	1 cut(s)	2118
BstUI	CGCG	3 cut(s)	127, 1770, 2338
BstV1I	GCAGC	6 cut(s)	764, 862, 1586, 1672, 2764, 3524
BstV2I	GAAGAC	3 cut(s)	1157, 2090, 3216
BstX2I	RGATCY	7 cut(s)	329, 1845, 2155, 2386, 2539, 3973, 4142
BstXI	CCANNNNNNTGG	2 cut(s)	2941, 3091
BstYI	RGATCY	7 cut(s)	329, 1845, 2155, 2386, 2539, 3973, 4142
BstZ17I	GTATAC	1 cut(s)	720
Bsu15I	ATCGAT	1 cut(s)	1561
Bsu36I	CCTNAGG	1 cut(s)	3891
BsuI	GTATCC	2 cut(s)	1468, 3896
BsuRI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BsuTUI	ATCGAT	1 cut(s)	1561
BtgI	CCRYGG	2 cut(s)	192, 1804
BtrI	CACGTC	1 cut(s)	3848
BtsI	GCAGTG	1 cut(s)	3426
BtsIMutI	CAGTG	8 cut(s)	240, 385, 1198, 1314, 2904, 2985, 3426, 4015
BtuMI	TCGCGA	1 cut(s)	1770
BveI	ACCTGC	9 cut(s)	292, 631, 1252, 1613, 2839, 3151, 3179, 3986, 4068
Cac8I	GCNNGC	7 cut(s)	264, 1143, 1216, 2246, 2338, 2930, 3510
CaiI	CAGNNNCTG	2 cut(s)	659, 3425
CciI	TCATGA	1 cut(s)	2371
CfoI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
Cfr13I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
ClaI	ATCGAT	1 cut(s)	1561
CseI	GACGC	2 cut(s)	91, 1438
Csp6I	GTAC	9 cut(s)	671, 745, 789, 1127, 1494, 2222, 2279, 2485, 3658
CspCI	CAANNNNNGTGG	2 cut(s)	2925, 2960
CviQI	GTAC	9 cut(s)	671, 745, 789, 1127, 1494, 2222, 2279, 2485, 3658
DraI	TTTAAA	1 cut(s)	1372
DraIII	CACNNNGTG	1 cut(s)	112
DrdI	GACNNNNNNGTC	1 cut(s)	1448
DseDI	GACNNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	2 cut(s)	1801, 2351
Eam1104I	CTCTTC	4 cut(s)	387, 657, 843, 4023
EarI	CTCTTC	4 cut(s)	387, 657, 843, 4023
EciI	GGCGGA	1 cut(s)	237
Ecl136II	GAGCTC	1 cut(s)	805
Eco130I	CCWWGG	3 cut(s)	192, 1050, 3569
Eco24I	GRGCYC	1 cut(s)	807
Eco32I	GATATC	2 cut(s)	349, 642
Eco47I	GGWCC	3 cut(s)	2427, 2468, 2725
Eco53kI	GAGCTC	1 cut(s)	805
Eco57I	CTGAAG	7 cut(s)	636, 681, 2274, 2772, 3560, 3646, 3756
Eco72I	CACGTG	1 cut(s)	1697
Eco81I	CCTNAGG	1 cut(s)	3891
EcoICRI	GAGCTC	1 cut(s)	805
EcoNI	CCTNNNNNAGG	2 cut(s)	2318, 3716
EcoRI	GAATTC	4 cut(s)	490, 918, 1833, 3351
EcoRII	CCWGG	7 cut(s)	266, 1260, 1527, 2340, 2511, 3574, 3841
EcoRV	GATATC	2 cut(s)	349, 642
EcoT14I	CCWWGG	3 cut(s)	192, 1050, 3569
EcoT22I	ATGCAT	2 cut(s)	1560, 3745
EcoT38I	GRGCYC	1 cut(s)	807
ErhI	CCWWGG	3 cut(s)	192, 1050, 3569
Esp3I	CGTCTC	1 cut(s)	2039
FalI	AAGNNNNNCTT	6 cut(s)	1173, 1205, 2082, 2114, 3318, 3350
FaqI	GGGAC	3 cut(s)	950, 1839, 4187
FauI	CCCGC	1 cut(s)	2648
FblI	GTMKAC	2 cut(s)	719, 1793
Fnu4HI	GCNGC	7 cut(s)	205, 753, 876, 1575, 1661, 2778, 3513
FriOI	GRGCYC	1 cut(s)	807
Fsp4HI	GCNGC	7 cut(s)	205, 753, 876, 1575, 1661, 2778, 3513
FspBI	CTAG	9 cut(s)	783, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
GlaI	GCGC	5 cut(s)	126, 2337, 2339, 2346, 4123
GluI	GCNGC	7 cut(s)	205, 753, 876, 1575, 1661, 2778, 3513
GsaI	CCCAGC	1 cut(s)	1149
GsuI	CTGGAG	2 cut(s)	1245, 1721
HaeII	RGCGCY	1 cut(s)	2348
HaeIII	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
HapII	CCGG	4 cut(s)	326, 495, 2241, 2813
HgaI	GACGC	2 cut(s)	91, 1438
HhaI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
Hin1I	GRCGYC	1 cut(s)	1449
Hin6I	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
HinP1I	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
HincII	GTYRAC	4 cut(s)	819, 1441, 4045, 4072
HindII	GTYRAC	4 cut(s)	819, 1441, 4045, 4072
HpaII	CCGG	4 cut(s)	326, 495, 2241, 2813
Hpy99I	CGWCG	1 cut(s)	1454
HpyCH4IV	ACGT	6 cut(s)	171, 1696, 2032, 3127, 3307, 3847
HpySE526I	ACGT	6 cut(s)	171, 1696, 2032, 3127, 3307, 3847
Hsp92I	GRCGYC	1 cut(s)	1449
HspAI	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
LguI	GCTCTTC	2 cut(s)	657, 843
LmnI	GCTCC	6 cut(s)	503, 810, 1430, 2708, 3296, 3833
Lsp1109I	GCAGC	6 cut(s)	764, 862, 1586, 1672, 2764, 3524
LweI	GCATC	7 cut(s)	933, 1567, 1591, 1909, 2789, 3175, 3555
MaeI	CTAG	9 cut(s)	783, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
MaeII	ACGT	6 cut(s)	171, 1696, 2032, 3127, 3307, 3847
MaeIII	GTNAC	5 cut(s)	112, 368, 434, 618, 1132
MfeI	CAATTG	1 cut(s)	2433
MflI	RGATCY	7 cut(s)	329, 1845, 2155, 2386, 2539, 3973, 4142
MhlI	GDGCHC	4 cut(s)	807, 2118, 2713, 3853
MlsI	TGGCCA	1 cut(s)	2353
MluNI	TGGCCA	1 cut(s)	2353
MlyI	GAGTC	6 cut(s)	365, 373, 1313, 2171, 3773, 3878
MmeI	TCCRAC	8 cut(s)	1215, 1491, 2197, 2854, 3274, 3328, 3414, 3811
Mox20I	TGGCCA	1 cut(s)	2353
Mph1103I	ATGCAT	2 cut(s)	1560, 3745
MroXI	GAANNNNTTC	2 cut(s)	2973, 3393
MscI	TGGCCA	1 cut(s)	2353
MseI	TTAA	9 cut(s)	236, 588, 615, 1371, 1392, 1566, 1955, 2585, 3807
MslI	CAYNNNNRTG	3 cut(s)	390, 441, 2148
Msp20I	TGGCCA	1 cut(s)	2353
MspA1I	CMGCKG	2 cut(s)	2777, 3508
MspI	CCGG	4 cut(s)	326, 495, 2241, 2813
MspR9I	CCNGG	8 cut(s)	268, 327, 1262, 1529, 2342, 2513, 3576, 3843
MunI	CAATTG	1 cut(s)	2433
Mva1269I	GAATGC	2 cut(s)	1383, 3743
MvaI	CCWGG	7 cut(s)	268, 1262, 1529, 2342, 2513, 3576, 3843
MvnI	CGCG	3 cut(s)	127, 1770, 2338
NciI	CCSGG	1 cut(s)	327
NcoI	CCATGG	1 cut(s)	192
NlaIV	GGNNCC	5 cut(s)	448, 499, 1239, 1426, 3193
NmuCI	GTSAC	3 cut(s)	112, 368, 434
NruI	TCGCGA	1 cut(s)	1770
NsiI	ATGCAT	2 cut(s)	1560, 3745
NspI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
NspV	TTCGAA	1 cut(s)	2821
OliI	CACNNNNGTG	1 cut(s)	390
PagI	TCATGA	1 cut(s)	2371
PalAI	GGCGCGCC	1 cut(s)	2336
PaqCI	CACCTGC	2 cut(s)	1252, 3151
PasI	CCCWGGG	1 cut(s)	1261
PauI	GCGCGC	1 cut(s)	2336
PciI	ACATGT	2 cut(s)	2287, 2580
PciSI	GCTCTTC	2 cut(s)	657, 843
PcsI	WCGNNNNNNNCGW	1 cut(s)	2056
PctI	GAATGC	2 cut(s)	1383, 3743
PdmI	GAANNNNTTC	2 cut(s)	2973, 3393
PflFI	GACNNNGTC	1 cut(s)	1583
PfoI	TCCNGGA	1 cut(s)	3841
PkrI	GCNGC	7 cut(s)	206, 754, 877, 1576, 1662, 2779, 3514
Ple19I	CGATCG	1 cut(s)	2050
PleI	GAGTC	6 cut(s)	365, 373, 1313, 2170, 3773, 3878
PmaCI	CACGTG	1 cut(s)	1697
PmlI	CACGTG	1 cut(s)	1697
PpsI	GAGTC	6 cut(s)	365, 373, 1313, 2170, 3773, 3878
Ppu21I	YACGTR	3 cut(s)	1697, 3128, 3308
PscI	ACATGT	2 cut(s)	2287, 2580
PshAI	GACNNNNGTC	1 cut(s)	3230
PshBI	ATTAAT	1 cut(s)	1955
PsiI	TTATAA	1 cut(s)	3932
Psp124BI	GAGCTC	1 cut(s)	807
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	7 cut(s)	266, 1260, 1527, 2340, 2511, 3574, 3841
PspCI	CACGTG	1 cut(s)	1697
PspFI	CCCAGC	1 cut(s)	1145
PspGI	CCWGG	7 cut(s)	266, 1260, 1527, 2340, 2511, 3574, 3841
PspN4I	GGNNCC	5 cut(s)	448, 499, 1239, 1426, 3193
PspPI	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
PsrI	GAACNNNNNNTAC	2 cut(s)	1110, 1142
PstNI	CAGNNNCTG	2 cut(s)	659, 3425
PsuI	RGATCY	7 cut(s)	329, 1845, 2155, 2386, 2539, 3973, 4142
PsyI	GACNNNGTC	1 cut(s)	1583
PteI	GCGCGC	1 cut(s)	2336
PvuI	CGATCG	1 cut(s)	2050
PvuII	CAGCTG	2 cut(s)	2777, 3508
RruI	TCGCGA	1 cut(s)	1770
RsaI	GTAC	9 cut(s)	672, 746, 790, 1128, 1495, 2223, 2280, 2486, 3659
RsaNI	GTAC	9 cut(s)	671, 745, 789, 1127, 1494, 2222, 2279, 2485, 3658
RseI	CAYNNNNRTG	3 cut(s)	390, 441, 2148
SacI	GAGCTC	1 cut(s)	807
SapI	GCTCTTC	2 cut(s)	657, 843
SaqAI	TTAA	9 cut(s)	236, 588, 615, 1371, 1392, 1566, 1955, 2585, 3807
SatI	GCNGC	7 cut(s)	205, 753, 876, 1575, 1661, 2778, 3513
Sau96I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
SchI	GAGTC	6 cut(s)	365, 373, 1313, 2171, 3773, 3878
ScrFI	CCNGG	8 cut(s)	268, 327, 1262, 1529, 2342, 2513, 3576, 3843
SduI	GDGCHC	4 cut(s)	807, 2118, 2713, 3853
SfaNI	GCATC	7 cut(s)	933, 1567, 1591, 1909, 2789, 3175, 3555
SfcI	CTRYAG	1 cut(s)	1341
SfuI	TTCGAA	1 cut(s)	2821
SgsI	GGCGCGCC	1 cut(s)	2336
SinI	GGWCC	3 cut(s)	2427, 2468, 2725
SmiMI	CAYNNNNRTG	3 cut(s)	390, 441, 2148
SmlI	CTYRAG	4 cut(s)	339, 2159, 2330, 3479
SmoI	CTYRAG	4 cut(s)	339, 2159, 2330, 3479
SsiI	CCGC	8 cut(s)	205, 248, 366, 946, 2430, 2641, 3518, 4084
SspI	AATATT	3 cut(s)	292, 3088, 3313
SspMI	CTAG	9 cut(s)	783, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
SstI	GAGCTC	1 cut(s)	807
StyD4I	CCNGG	8 cut(s)	266, 325, 1260, 1527, 2340, 2511, 3574, 3841
StyI	CCWWGG	3 cut(s)	192, 1050, 3569
TaiI	ACGT	6 cut(s)	174, 1699, 2035, 3130, 3310, 3850
TaqI	TCGA	9 cut(s)	282, 549, 1452, 1561, 1837, 2821, 3952, 3978, 4128
TatI	WGTACW	4 cut(s)	670, 2221, 2278, 2484
TauI	GCSGC	1 cut(s)	207
Tru1I	TTAA	9 cut(s)	236, 588, 615, 1371, 1392, 1566, 1955, 2585, 3807
Tru9I	TTAA	9 cut(s)	236, 588, 615, 1371, 1392, 1566, 1955, 2585, 3807
TscAI	CASTG	8 cut(s)	247, 392, 1205, 1321, 2911, 2992, 3426, 4015
TseFI	GTSAC	3 cut(s)	112, 368, 434
TseI	GCWGC	6 cut(s)	752, 875, 1574, 1660, 2777, 3512
Tsp45I	GTSAC	3 cut(s)	112, 368, 434
TspGWI	ACGGA	8 cut(s)	341, 1742, 1795, 2048, 2753, 3327, 3477, 4089
TspRI	CASTG	8 cut(s)	247, 392, 1205, 1321, 2911, 2992, 3426, 4015
Tth111I	GACNNNGTC	1 cut(s)	1583
VpaK11BI	GGWCC	3 cut(s)	2427, 2468, 2725
VspI	ATTAAT	1 cut(s)	1955
XagI	CCTNNNNNAGG	2 cut(s)	2318, 3716
XbaI	TCTAGA	1 cut(s)	2503
XceI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2941
XmiI	GTMKAC	2 cut(s)	719, 1793
XmnI	GAANNNNTTC	2 cut(s)	2973, 3393
XspI	CTAG	9 cut(s)	783, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
Zsp2I	ATGCAT	2 cut(s)	1560, 3745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.