pycom16g13180

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009388
Physical Location & Seq
Forward (+)
9370219 .. 9372107
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGGTGCCTGCTTGGACCGGCCCATTAATATCTGAAACAGTAGCTGGCCCAACGATCACAAGCGGTGGCTACTGCCGACTGCTTCTACACCCCCTCCCTCCATCTCTATCTCTCTATCAGTCATTCGGGCGGTCAAATGCGAAACAGAGAAACCACAAGGAAAGGAAGAGATCGACGCCGCAGAAAATGGATGGGAAGCCGAAAGCTGGAGTATCGATGACCAACACGTGGCTGAGGAAGCACCGTCTGATCTACATTGGAGCCACCCGACACCCCTTCATCATCAGCATTCGCGACGGCACCGTCGATCTTTCCGCCTTCAAACGCTGGCTGGGCCAGGACTACATATTTGTGAGAGCATTTGTTCCATTTGTGGCGAGTGTGCTGATAAAAGCTTGGAAGAATTCCGATCACGAAAGCGGCGATATCGAATTGGTACTGAGTGGGGTGGCTGCTCTGAATGATGAGATTGAATGGTTCAAGCAAGAGGCTTCCAAGTGGGGCGTTGAGCTATCCGGTGTTGTTCCTGAAAAAACAACCCAGGATTACTGCAGATTTCTGGAGGATCTAATGAGCCCGGATGTTGATTACGCTGTGGCCATGACAGCCTTTTGGGCCATCGAAGCTGTATACCAAGAGAGCTTTGCCCATTGCCTGGAAGAAGGGTCCAAAACCCCACCAGAACTGCAAGAGGCTTGCCAAAGGTGGGGCAATGATGGCTTCGGCCAGTACTGCTCTTCTCTCCGAAACATTGCTGACCGAGAGTTGGAGAAGGCCGCCTCAGACGGCGGGCCTTTGGTGAAGGTTTCAGAAGATGTGGTCACCAAGGCTGAAGTAGCGTTCCTGCATGTACTCGAGTATGAGGTTCAGTTTTGGAACATGAGCCATGGGAGTCCTGGACGCTCTGCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

305

Amino Acids

33.99

Weight (kDa)

6.09

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 79 - 295 9.3e-39 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 4, 299
AccB7I CCANNNNNTGG 2 cut(s) 228, 655
AccI GTMKAC 1 cut(s) 630
AccII CGCG 1 cut(s) 294
AciI CCGC 7 cut(s) 63, 130, 179, 315, 420, 777, 789
AclWI GGATC 1 cut(s) 573
AcoI YGGCCR 2 cut(s) 597, 724
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 1 cut(s) 852
AcvI CACGTG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 3 cut(s) 438, 731, 852
AfiI CCNNNNNNNGG 5 cut(s) 206, 228, 655, 706, 766
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 322, 473, 481
AjnI CCWGG 4 cut(s) 336, 540, 654, 895
AluBI AGCT 6 cut(s) 44, 206, 395, 511, 626, 642
AluI AGCT 6 cut(s) 44, 206, 395, 511, 626, 642
AlwI GGATC 1 cut(s) 573
AlwNI CAGNNNCTG 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 8 cut(s) 19, 46, 334, 597, 615, 724, 774, 791
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 403
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AseI ATTAAT 1 cut(s) 26
AspS9I GGNCC 7 cut(s) 15, 20, 47, 334, 615, 666, 791
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 811, 814
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 2 cut(s) 15, 666
BalI TGGCCA 1 cut(s) 599
BanI GGYRCC 2 cut(s) 4, 299
BanII GRGCYC 1 cut(s) 578
BbrPI CACGTG 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 439
BccI CCATC 4 cut(s) 109, 185, 626, 710
BceAI ACGGC 2 cut(s) 313, 802
BciT130I CCWGG 4 cut(s) 338, 542, 656, 897
BcnI CCSGG 1 cut(s) 578
BfmI CTRYAG 1 cut(s) 550
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 4 cut(s) 179, 421, 453, 777
BlsI GCNGC 4 cut(s) 180, 422, 454, 778
BmcAI AGTACT 1 cut(s) 731
Bme1390I CCNGG 5 cut(s) 338, 542, 578, 656, 897
Bme18I GGWCC 2 cut(s) 15, 666
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 7 cut(s) 15, 20, 47, 334, 615, 666, 791
BmiI GGNNCC 4 cut(s) 6, 262, 301, 667
BmrFI CCNGG 5 cut(s) 338, 542, 578, 656, 897
BpmI CTGGAG 2 cut(s) 228, 581
Bpu10I CCTNAGC 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 215
BsaAI YACGTR 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 540, 825, 886
BsaWI WCCGGW 1 cut(s) 515
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 228, 655, 706, 766
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 3 cut(s) 649, 718, 750
BseBI CCWGG 4 cut(s) 338, 542, 656, 897
BseCI ATCGAT 1 cut(s) 215
BseDI CCNNGG 3 cut(s) 540, 825, 886
BseGI GGATG 2 cut(s) 196, 586
BseLI CCNNNNNNNGG 5 cut(s) 206, 228, 655, 706, 766
BseMI GCAATG 3 cut(s) 649, 718, 750
BseMII CTCAG 3 cut(s) 224, 431, 795
BseNI ACTGG 1 cut(s) 727
BseXI GCAGC 1 cut(s) 439
BseYI CCCAGC 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 8 cut(s) 21, 48, 336, 599, 617, 726, 776, 793
BshNI GGYRCC 2 cut(s) 4, 299
BshVI ATCGAT 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 18, 516, 578
BslI CCNNNNNNNGG 5 cut(s) 206, 228, 655, 706, 766
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 8 cut(s) 21, 48, 336, 599, 617, 726, 776, 793
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 1 cut(s) 578
Bsp143I GATC 6 cut(s) 54, 170, 249, 307, 409, 565
Bsp19I CCATGG 1 cut(s) 886
Bsp68I TCGCGA 1 cut(s) 294
BspACI CCGC 7 cut(s) 63, 130, 179, 315, 420, 777, 789
BspANI GGCC 8 cut(s) 21, 48, 336, 599, 617, 726, 776, 793
BspCNI CTCAG 3 cut(s) 225, 432, 794
BspDI ATCGAT 1 cut(s) 215
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 4 cut(s) 6, 262, 301, 667
BspMAI CTGCAG 1 cut(s) 554
BspPI GGATC 1 cut(s) 573
BspQI GCTCTTC 1 cut(s) 742
BspT107I GGYRCC 2 cut(s) 4, 299
BsrDI GCAATG 3 cut(s) 649, 718, 750
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 3 cut(s) 540, 825, 886
BssMI GATC 6 cut(s) 54, 170, 249, 307, 409, 565
BssNAI GTATAC 1 cut(s) 631
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 825, 886
Bst1107I GTATAC 1 cut(s) 631
Bst2UI CCWGG 4 cut(s) 338, 542, 656, 897
Bst4CI ACNGT 3 cut(s) 40, 245, 304
Bst6I CTCTTC 2 cut(s) 161, 742
BstACI GRCGYC 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 5 cut(s) 9, 46, 329, 697, 791
BstDEI CTNAG 3 cut(s) 233, 440, 781
BstDSI CCRYGG 1 cut(s) 886
BstEII GGTNACC 1 cut(s) 820
BstF5I GGATG 2 cut(s) 196, 586
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 6 cut(s) 57, 173, 252, 310, 412, 568
BstMBI GATC 6 cut(s) 54, 170, 249, 307, 409, 565
BstMWI GCNNNNNNNGC 8 cut(s) 238, 333, 605, 614, 623, 717, 732, 836
BstNI CCWGG 4 cut(s) 338, 542, 656, 897
BstNSI RCATGY 1 cut(s) 851
BstPI GGTNACC 1 cut(s) 820
BstSCI CCNGG 5 cut(s) 336, 540, 576, 654, 895
BstSFI CTRYAG 1 cut(s) 550
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BstZ17I GTATAC 1 cut(s) 631
Bsu15I ATCGAT 1 cut(s) 215
BsuRI GGCC 8 cut(s) 21, 48, 336, 599, 617, 726, 776, 793
BsuTUI ATCGAT 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 2 cut(s) 196, 586
BtuMI TCGCGA 1 cut(s) 294
Cac8I GCNNGC 5 cut(s) 9, 46, 329, 697, 791
CaiI CAGNNNCTG 1 cut(s) 44
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 7 cut(s) 15, 20, 47, 334, 615, 666, 791
ClaI ATCGAT 1 cut(s) 215
CseI GACGC 2 cut(s) 184, 909
Csp6I GTAC 3 cut(s) 437, 730, 851
CviAII CATG 5 cut(s) 601, 848, 880, 887, 912
CviQI GTAC 3 cut(s) 437, 730, 851
DdeI CTNAG 3 cut(s) 233, 440, 781
DpnI GATC 6 cut(s) 56, 172, 251, 309, 411, 567
DpnII GATC 6 cut(s) 54, 170, 249, 307, 409, 565
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 2 cut(s) 597, 724
Eam1104I CTCTTC 2 cut(s) 161, 742
EarI CTCTTC 2 cut(s) 161, 742
EciI GGCGGA 1 cut(s) 304
Eco130I CCWWGG 2 cut(s) 825, 886
Eco24I GRGCYC 1 cut(s) 578
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 15, 666
Eco57I CTGAAG 1 cut(s) 852
Eco72I CACGTG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 854
Eco91I GGTNACC 1 cut(s) 820
EcoO65I GGTNACC 1 cut(s) 820
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 4 cut(s) 336, 540, 654, 895
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 825, 886
EcoT38I GRGCYC 1 cut(s) 578
ErhI CCWWGG 2 cut(s) 825, 886
FaeI CATG 5 cut(s) 604, 851, 883, 890, 915
FaiI YATR 8 cut(s) 347, 602, 631, 849, 861, 881, 888, 913
FatI CATG 5 cut(s) 600, 847, 879, 886, 911
FauI CCCGC 1 cut(s) 782
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 4 cut(s) 179, 421, 453, 777
FokI GGATG 2 cut(s) 203, 593
FriOI GRGCYC 1 cut(s) 578
Fsp4HI GCNGC 4 cut(s) 179, 421, 453, 777
GluI GCNGC 4 cut(s) 179, 421, 453, 777
GsaI CCCAGC 1 cut(s) 335
GsuI CTGGAG 2 cut(s) 228, 581
HaeIII GGCC 8 cut(s) 21, 48, 336, 599, 617, 726, 776, 793
HapII CCGG 3 cut(s) 18, 516, 578
HgaI GACGC 2 cut(s) 184, 909
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 5 cut(s) 604, 851, 883, 890, 915
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 1 cut(s) 892
HpaII CCGG 3 cut(s) 18, 516, 578
HphI GGTGA 2 cut(s) 811, 814
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 7 cut(s) 34, 249, 409, 459, 746, 784, 811
Hpy188III TCNNGA 4 cut(s) 293, 413, 527, 560
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 3 cut(s) 178, 299, 308
HpyAV CCTTC 5 cut(s) 286, 328, 656, 766, 796
HpyCH4III ACNGT 3 cut(s) 40, 245, 304
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 3 cut(s) 552, 688, 847
HpyF10VI GCNNNNNNNGC 8 cut(s) 238, 333, 605, 614, 623, 717, 732, 836
HpyF3I CTNAG 3 cut(s) 233, 440, 781
HpySE526I ACGT 1 cut(s) 227
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 5 cut(s) 604, 851, 883, 890, 915
Kzo9I GATC 6 cut(s) 54, 170, 249, 307, 409, 565
LguI GCTCTTC 1 cut(s) 742
LmnI GCTCC 2 cut(s) 260, 913
Lsp1109I GCAGC 1 cut(s) 439
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 1 cut(s) 820
MalI GATC 6 cut(s) 56, 172, 251, 309, 411, 567
MboI GATC 6 cut(s) 54, 170, 249, 307, 409, 565
MboII GAAGA 5 cut(s) 178, 412, 671, 729, 824
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 578
MlsI TGGCCA 1 cut(s) 599
MluCI AATT 2 cut(s) 403, 431
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 1 cut(s) 901
MmeI TCCRAC 1 cut(s) 747
MnlI CCTC 8 cut(s) 104, 108, 228, 481, 556, 685, 790, 856
Mox20I TGGCCA 1 cut(s) 599
MscI TGGCCA 1 cut(s) 599
MseI TTAA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 599
MspI CCGG 3 cut(s) 18, 516, 578
MspR9I CCNGG 5 cut(s) 338, 542, 578, 656, 897
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 4 cut(s) 338, 542, 656, 897
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 8 cut(s) 238, 333, 605, 614, 623, 717, 732, 836
NciI CCSGG 1 cut(s) 578
NcoI CCATGG 1 cut(s) 886
NdeII GATC 6 cut(s) 54, 170, 249, 307, 409, 565
NlaIII CATG 5 cut(s) 604, 851, 883, 890, 915
NlaIV GGNNCC 4 cut(s) 6, 262, 301, 667
NmuCI GTSAC 1 cut(s) 820
NruI TCGCGA 1 cut(s) 294
NspI RCATGY 1 cut(s) 851
PaeR7I CTCGAG 1 cut(s) 854
PciSI GCTCTTC 1 cut(s) 742
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 420
PctI GAATGC 1 cut(s) 288
PflMI CCANNNNNTGG 2 cut(s) 228, 655
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 4 cut(s) 180, 422, 454, 778
PleI GAGTC 1 cut(s) 900
PmaCI CACGTG 1 cut(s) 228
PmlI CACGTG 1 cut(s) 228
PpsI GAGTC 1 cut(s) 900
Ppu21I YACGTR 1 cut(s) 228
PshBI ATTAAT 1 cut(s) 26
Psp6I CCWGG 4 cut(s) 336, 540, 654, 895
PspCI CACGTG 1 cut(s) 228
PspEI GGTNACC 1 cut(s) 820
PspFI CCCAGC 1 cut(s) 331
PspGI CCWGG 4 cut(s) 336, 540, 654, 895
PspN4I GGNNCC 4 cut(s) 6, 262, 301, 667
PspPI GGNCC 7 cut(s) 15, 20, 47, 334, 615, 666, 791
PspXI VCTCGAGB 1 cut(s) 854
PstI CTGCAG 1 cut(s) 554
PstNI CAGNNNCTG 1 cut(s) 44
PsuI RGATCY 1 cut(s) 565
RruI TCGCGA 1 cut(s) 294
RsaI GTAC 3 cut(s) 438, 731, 852
RsaNI GTAC 3 cut(s) 437, 730, 851
RseI CAYNNNNRTG 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 742
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 4 cut(s) 179, 421, 453, 777
Sau3AI GATC 6 cut(s) 54, 170, 249, 307, 409, 565
Sau96I GGNCC 7 cut(s) 15, 20, 47, 334, 615, 666, 791
ScaI AGTACT 1 cut(s) 731
SchI GAGTC 1 cut(s) 901
ScrFI CCNGG 5 cut(s) 338, 542, 578, 656, 897
SduI GDGCHC 1 cut(s) 578
SfcI CTRYAG 1 cut(s) 550
Sfr274I CTCGAG 1 cut(s) 854
SinI GGWCC 2 cut(s) 15, 666
SlaI CTCGAG 1 cut(s) 854
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 2 cut(s) 403, 431
SsiI CCGC 7 cut(s) 63, 130, 179, 315, 420, 777, 789
StyD4I CCNGG 5 cut(s) 336, 540, 576, 654, 895
StyI CCWWGG 2 cut(s) 825, 886
TaaI ACNGT 3 cut(s) 40, 245, 304
TaiI ACGT 1 cut(s) 230
TaqI TCGA 6 cut(s) 173, 215, 306, 429, 621, 855
TaqII GACCGA 1 cut(s) 774
TasI AATT 2 cut(s) 403, 431
TatI WGTACW 2 cut(s) 729, 850
TauI GCSGC 3 cut(s) 181, 423, 779
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 1 cut(s) 268
Van91I CCANNNNNTGG 2 cut(s) 228, 655
VpaK11BI GGWCC 2 cut(s) 15, 666
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 1 cut(s) 403
XceI RCATGY 1 cut(s) 851
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XhoI CTCGAG 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 630
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.