Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0004385

Physical Location & Seq

Genomic Coordinates Reverse (-)

11046829 .. 11053519

Gene Structure 6691 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGCTCATAAGGACTCCAGCTGAGGTTTATAGGCAGAGATTGTTCAAGGGAAAATATGTGGATCAATTTGATCTTGTTAGAGAAAACGCTTCCTTCCATGGTCTCAACTCGTCGGGTCGAGTTGTCCTCAAGCGCTGTCACCCGCAGCAGATGCAAATTATAAACACTATCACAAATCCAAACCCGAAGAGGAACGAGCCAGGACACATCGAAGCAAAAGATGTTCTGTCCCCAGTCGCAGATCAGACTGCACCAACAACCAGAAAGCGAGTATTTACTTTTGGAAAAGGAAAGAGTGAAGGCAACAAGGCCATGAAGTCATTGTTGGGAGGGAAAGGTGCAAACCTGGCGGAAATGGCTAGCATTGGGCTATCCGTCCCACCTGGACTTACTATTTCAACAGAAGCATGCCAAGAGTATCAGGTCAATGGCAAAGATTTACCACAAGGTTTGTGGGAGGAGATATTAGAGGGCTTAGAGAGTGTGCAGAAGGACATGGGTGCTATTCTCGGTGACCCTGCCAAGCCTCTACTCCTCTCCGTTCGCTCTGGTGCTGCGATTTCCATGCCCGGTATGATGGACACTGTCCTCAACCTTGGACTGAATGATGAGGTGGTTGCTGGTTTGGCCGCAAAAAGTGGAGAGCGCTTTGCTTATGACTCGTACAGACGTTTTCTAGACATGTTTGGAGATGTTGTTATGGGAATTCCACACTCATCATTCGAGGAGCAGTTGGAAAAACTAAAAGGTACGAAAGGAGTTGAGCTTGACACAAAGCTAACAGCCTCTGATCTTAAAGAACTGGTAGAACAATACAAAAATGTGTATCTCGAAACCACGGGTGAAAAGTTCCCTTCAGGTACAAACTCACCTCTTTATGTTAATTTGTACTTGTCCCCATGGATATCTGCAGATCCAAAACAACAGCTACTGTTGGCTATCAAAGCAGTTTTTGATTCATGGGACAGTCCAAGGGCCAATAAATACCGGAGCATCAATCAGATAACCGGTTTGAAGGGAACCGCAGTTAACATTCAATGCATGGTTTTTGGGAACATGGGAAATACTTCAGGGACAGGTGTTTTGTTCACTAGGAATCCAAGCACCGGAGAAAGGAAGCTTTATGGCGAATTTTTAATCAATGCTCAGGGAGAAGATGTAGTTGCCGGCATTAGGACGCCAGAAGACTTGGATACTATGAAAAATTGTATGCCAGAAGCTTATGAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAAGCATTACAAAGATATGATGGACATTGAGTTCACAGTCCAAGAAAATAGGTTGTGGATGTTACAATGCCGCGCTGGAAAGCGTACTGGTAAAGGTGCGGTTAAGATAGCTGTAGACATGGTGAACGAAGGGCTTGTGGATAAGCGTGCTGCAATCAAGATGGTGGAACCACAGCACCTGGACCAACTTCTTCACCCACAGTTTGAGAATCCAACCGCTTACAAAGACAAAGTGATCGCCACTGGATTACCAGCATCTCCAGGAGCAGCCGTAGGGACAGTCGTATTCAGTGCTGATGATGCTGAAGCATGGCATGCACAAGGAAAGAGTGTCATCCTGGTAAGAACAGAGACTAGCCCCGAAGATGTTGGAGGTATGCATGCTGCTGCAGGGATCTTGACAGCTAGAGGTGGCATGACATCTCATGCAGCCGTTGTAGCCCGTGGGTGGGGCAAATGTTGTGTTTCTGGCTGCGCTGACATCCGAGTAAATGACGCTGAGAAGGTGGTTTACATTCTGGTTGTGATCGGGAATACAGTGGTCAATGAAGGAGAATGGCTTTCACTCAACGGATCCACTGGCGAAGTCATATTAGGAAAACAGCCACTTTCTCCTCCCGCTTTAAGTGGCGATTTGGAGACCTTCATGTCTTGGGCTGATAAAGTCCGGCGTCTCAAGGTTATGGCAAACGCGGACACACCTGAAGATGCTCTAACAGCAAGAAATAACGGTGCGGAAGGGATTGGACTTTGCAGGACAGAGCACATGATTTGTAATTGTTGGCTATCTATCATAATGTCAGGTCTTCCAGTAACAATCCGCCTGTTAGACCCTCCACTTCATGAATTTCTTCCAGAAGGTGACTTAGAACAGATTGTCGGCGAACTATCTGCAGAGACTGGCATGACTGAGGATGAAGTATTCTCAAGAATTGAGAAATTATCAGAAGTAAACCCCATGCTTGGTTTCCGCGGCTGCAGGTTGGGGATATCATATCCAGAACTCACCGAAATGCAGACGCGTGCAATCTTTCAAGCCGCAGTCTCAATGAGCAACCAGGGTGTCAAAGTTTTCCCCGAGATAATGGTTCCCCTTGTCGGAACACCTCAGGAACTAGGACATCAAGTGAGACTCATTCACAGTGTTGCGGTCAAGGTGTTCTCCGAGATGGATACTACCTTGAGTTATAAGGTTGGGACTATGATTGAGATCCCTAGAGCTGCTCTGGTTGCAGATGAGGTAAGCACACAAATGACATTTGGCTACAGTAGAGATGATGTGGGCAAGTTTCTACCTATCTACCTTGCGAAAGGCCTCCTGTCTCAAATTTCGATCCATTTGAGGTTAGTCACATTTATGCAAGTCCACGCAGGCTTCGTTGTAGATAGGTTTTTCCCGAATAGGTTCGTTTGCTTTTATCTATCTTATTTAGGCTGCATAAACTACTTTTATAACCAGATCAAATTCCTGATCCTAAATTCTTTGACAGGGGCAACTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEP-utilizers PEP-utilizers_C PPDK_N

Protein Analysis

920

Amino Acids

100.81

Weight (kDa)

5.63

Isoelectric Point (pI)

33.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPDK_N	PF01326	107 - 164	1.6e-07	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	145 - 397	7.5e-23	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	408 - 462	1.3e-10	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers	PF00391	528 - 613	1.7e-22	PEP-utilising enzyme, mobile domain
PEP-utilizers_C	PF02896	628 - 839	1.4e-44	PEP-utilising enzyme, PEP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530
fragaria_vesca	FvH4_4g16670
malus_domestica	MD13G1177400.v1.1 MD13G1177500.v1.1 MD16G1179300.v1.1 MD16G1179400.v1.1 MD16G1179500.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15300 pycom16g15050 pycom16g15060 pycom16g15070
rosa_chinensis	RchiOBHm_Chr4g0420131
rosa_laevigata	RLG00000007786
rosa_multiflora	Rmu_sc0001252.1_g000003 Rmu_sc0025222.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362840
rosa_rugosa	Rorug04G0162400 Rorug04G0162500 Rorug04G0162600 Rorug04G0162600
rosa_samantha	Rh4AG223300 Rh4BG225700 Rh4CG236500 Rh4DG222100
rosa_wichuraiana	Rw4G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	161, 2445, 2709
AasI	GACNNNNNNGTC	1 cut(s)	1903
Acc36I	ACCTGC	1 cut(s)	2226
AccI	GTMKAC	1 cut(s)	1371
AccII	CGCG	4 cut(s)	1329, 1949, 2229, 2278
AclWI	GGATC	8 cut(s)	69, 908, 1658, 1824, 1837, 2461, 2584, 2722
AcoI	YGGCCR	1 cut(s)	627
AcsI	RAATTY	7 cut(s)	705, 1130, 1248, 2102, 2583, 2720, 2734
AcuI	CTGAAG	4 cut(s)	840, 1053, 1581, 1980
AcyI	GRCGYC	2 cut(s)	1178, 1927
AfaI	GTAC	5 cut(s)	665, 751, 862, 890, 1342
AfeI	AGCGCT	2 cut(s)	134, 647
AfiI	CCNNNNNNNGG	7 cut(s)	1106, 1113, 1173, 1704, 1705, 2219, 2354
AflIII	ACRYGT	2 cut(s)	681, 2276
AgeI	ACCGGT	1 cut(s)	1007
AgsI	TTSAA	5 cut(s)	46, 399, 1015, 1037, 2291
AjnI	CCWGG	7 cut(s)	199, 345, 382, 1434, 1516, 1593, 2313
AjuI	GAANNNNNNNTTGG	2 cut(s)	308, 340
AloI	GAACNNNNNNTCC	2 cut(s)	1271, 1303
Alw21I	GWGCWC	1 cut(s)	2022
Alw26I	GTCTC	8 cut(s)	107, 1601, 1889, 1934, 2147, 2305, 2380, 2583
AlwI	GGATC	8 cut(s)	69, 908, 1658, 1824, 1837, 2461, 2584, 2722
AlwNI	CAGNNNCTG	4 cut(s)	788, 931, 1435, 2156
Ama87I	CYCGRG	1 cut(s)	2333
Aor51HI	AGCGCT	2 cut(s)	134, 647
AoxI	GGCC	4 cut(s)	309, 627, 975, 2569
ApoI	RAATTY	7 cut(s)	705, 1130, 1248, 2102, 2583, 2720, 2734
ArsI	GACNNNNNNTTYG	2 cut(s)	1066, 1098
AsiGI	ACCGGT	1 cut(s)	1007
Asp700I	GAANNNNTTC	3 cut(s)	1066, 2106, 2660
AspLEI	GCGC	4 cut(s)	135, 648, 1331, 1733
AspS9I	GGNCC	2 cut(s)	975, 1438
AsuC2I	CCSGG	1 cut(s)	570
AsuHPI	GGTGA	8 cut(s)	131, 524, 854, 861, 1390, 1442, 2129, 2254
AsuNHI	GCTAGC	1 cut(s)	359
AvaI	CYCGRG	1 cut(s)	2333
AvaII	GGWCC	1 cut(s)	1438
AxyI	CCTNAGG	1 cut(s)	2364
BamHI	GGATCC	1 cut(s)	1829
BarI	GAAGNNNNNNTAC	2 cut(s)	1751, 1783
BbsI	GAAGAC	2 cut(s)	1191, 2054
Bbv12I	GWGCWC	1 cut(s)	2022
BbvCI	CCTCAGC	1 cut(s)	21
BccI	CCATC	4 cut(s)	571, 1270, 1411, 2419
BceAI	ACGGC	2 cut(s)	1511, 1673
BcgI	CGANNNNNNTGC	4 cut(s)	547, 581, 2129, 2163
BciT130I	CCWGG	7 cut(s)	201, 347, 384, 1436, 1518, 1595, 2315
BciVI	GTATCC	2 cut(s)	1186, 2422
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	8 cut(s)	107, 1601, 1889, 1934, 2147, 2305, 2380, 2583
BfaI	CTAG	8 cut(s)	360, 677, 1092, 1253, 1611, 1662, 2372, 2472
BfmI	CTRYAG	6 cut(s)	909, 1368, 1644, 2148, 2233, 2521
BfoI	RGCGCY	2 cut(s)	136, 649
BfuAI	ACCTGC	1 cut(s)	2226
BfuI	GTATCC	2 cut(s)	1186, 2422
Bme1390I	CCNGG	8 cut(s)	201, 347, 384, 570, 1436, 1518, 1595, 2315
Bme18I	GGWCC	1 cut(s)	1438
BmeT110I	CYCGRG	1 cut(s)	2333
BmgT120I	GGNCC	2 cut(s)	975, 1438
BmiI	GGNNCC	4 cut(s)	1021, 1425, 1831, 2346
BmrFI	CCNGG	8 cut(s)	201, 347, 384, 570, 1436, 1518, 1595, 2315
BmrI	ACTGGG	1 cut(s)	227
BmsI	GCATC	5 cut(s)	141, 1002, 1520, 1546, 1954
BmtI	GCTAGC	1 cut(s)	363
BmuI	ACTGGG	1 cut(s)	227
BpiI	GAAGAC	2 cut(s)	1191, 2054
BplI	GAGNNNNNCTC	2 cut(s)	111, 143
BpmI	CTGGAG	1 cut(s)	1500
Bpu10I	CCTNAGC	2 cut(s)	21, 1146
BpuEI	CTTGAG	4 cut(s)	113, 1916, 2167, 2458
BpuMI	CCSGG	1 cut(s)	570
BsaBI	GATNNNNATC	1 cut(s)	2465
BsaHI	GRCGYC	2 cut(s)	1178, 1927
BsaI	GGTCTC	2 cut(s)	107, 1889
BsaJI	CCNNGG	8 cut(s)	97, 595, 837, 899, 971, 1699, 2227, 2314
BsaWI	WCCGGW	3 cut(s)	987, 1007, 1106
BsaXI	ACNNNNNCTCC	6 cut(s)	681, 711, 1143, 1173, 1512, 1542
Bsc4I	CCNNNNNNNGG	7 cut(s)	1106, 1113, 1173, 1704, 1705, 2219, 2354
Bse118I	RCCGGY	2 cut(s)	1007, 1166
Bse1I	ACTGG	7 cut(s)	233, 807, 1348, 1504, 1840, 2066, 2161
Bse21I	CCTNAGG	1 cut(s)	2364
Bse8I	GATNNNNATC	1 cut(s)	2465
BseBI	CCWGG	7 cut(s)	201, 347, 384, 1436, 1518, 1595, 2315
BseDI	CCNNGG	8 cut(s)	97, 595, 837, 899, 971, 1699, 2227, 2314
BseGI	GGATG	4 cut(s)	1320, 1590, 1737, 2176
BseJI	GATNNNNATC	1 cut(s)	2465
BseLI	CCNNNNNNNGG	7 cut(s)	1106, 1113, 1173, 1704, 1705, 2219, 2354
BseMII	CTCAG	5 cut(s)	12, 1160, 1746, 2157, 2378
BseNI	ACTGG	7 cut(s)	233, 807, 1348, 1504, 1840, 2066, 2161
BseRI	GAGGAG	5 cut(s)	473, 524, 740, 1241, 1860
BsgI	GTGCAG	2 cut(s)	234, 506
Bsh1236I	CGCG	4 cut(s)	1329, 1949, 2229, 2278
BshFI	GGCC	4 cut(s)	311, 629, 977, 2571
BshTI	ACCGGT	1 cut(s)	1007
BsiHKAI	GWGCWC	1 cut(s)	2022
BsiHKCI	CYCGRG	1 cut(s)	2333
BsiSI	CCGG	6 cut(s)	570, 988, 1008, 1107, 1167, 1924
BslFI	GGGAC	7 cut(s)	214, 362, 880, 977, 1087, 1546, 2467
BslI	CCNNNNNNNGG	7 cut(s)	1106, 1113, 1173, 1704, 1705, 2219, 2354
BsmAI	GTCTC	8 cut(s)	107, 1601, 1889, 1934, 2147, 2305, 2380, 2583
BsmBI	CGTCTC	1 cut(s)	1934
BsmFI	GGGAC	7 cut(s)	214, 362, 880, 977, 1087, 1546, 2467
BsnI	GGCC	4 cut(s)	311, 629, 977, 2571
Bso31I	GGTCTC	2 cut(s)	107, 1889
BsoBI	CYCGRG	1 cut(s)	2333
Bsp1286I	GDGCHC	1 cut(s)	2022
Bsp19I	CCATGG	2 cut(s)	97, 899
BspANI	GGCC	4 cut(s)	311, 629, 977, 2571
BspCNI	CTCAG	5 cut(s)	13, 1159, 1747, 2158, 2377
BspFNI	CGCG	4 cut(s)	1329, 1949, 2229, 2278
BspHI	TCATGA	1 cut(s)	2098
BspLI	GGNNCC	4 cut(s)	1021, 1425, 1831, 2346
BspMAI	CTGCAG	4 cut(s)	913, 1648, 2152, 2237
BspMI	ACCTGC	1 cut(s)	2226
BspOI	GCTAGC	1 cut(s)	363
BspPI	GGATC	8 cut(s)	69, 908, 1658, 1824, 1837, 2461, 2584, 2722
BspTNI	GGTCTC	2 cut(s)	107, 1889
BsrFI	RCCGGY	2 cut(s)	1007, 1166
BsrI	ACTGG	7 cut(s)	233, 807, 1348, 1504, 1840, 2066, 2161
BssAI	RCCGGY	2 cut(s)	1007, 1166
BssECI	CCNNGG	8 cut(s)	97, 595, 837, 899, 971, 1699, 2227, 2314
BssNI	GRCGYC	2 cut(s)	1178, 1927
BssT1I	CCWWGG	4 cut(s)	97, 595, 899, 971
Bst2UI	CCWGG	7 cut(s)	201, 347, 384, 1436, 1518, 1595, 2315
Bst6I	CTCTTC	1 cut(s)	182
BstACI	GRCGYC	2 cut(s)	1178, 1927
BstAPI	GCANNNNNTGC	2 cut(s)	151, 1571
BstC8I	GCNNGC	8 cut(s)	361, 409, 1168, 1404, 1572, 1638, 2280, 2629
BstDEI	CTNAG	7 cut(s)	21, 475, 1146, 1755, 2122, 2166, 2364
BstDSI	CCRYGG	5 cut(s)	97, 837, 899, 1699, 2227
BstEII	GGTNACC	1 cut(s)	512
BstF5I	GGATG	4 cut(s)	1320, 1590, 1737, 2176
BstFNI	CGCG	4 cut(s)	1329, 1949, 2229, 2278
BstH2I	RGCGCY	2 cut(s)	136, 649
BstHHI	GCGC	4 cut(s)	135, 648, 1331, 1733
BstMAI	GTCTC	8 cut(s)	107, 1601, 1889, 1934, 2147, 2305, 2380, 2583
BstNI	CCWGG	7 cut(s)	201, 347, 384, 1436, 1518, 1595, 2315
BstNSI	RCATGY	4 cut(s)	411, 685, 1574, 1640
BstPI	GGTNACC	1 cut(s)	512
BstSCI	CCNGG	8 cut(s)	199, 345, 382, 568, 1434, 1516, 1593, 2313
BstSFI	CTRYAG	6 cut(s)	909, 1368, 1644, 2148, 2233, 2521
BstUI	CGCG	4 cut(s)	1329, 1949, 2229, 2278
BstV2I	GAAGAC	2 cut(s)	1191, 2054
BstX2I	RGATCY	4 cut(s)	913, 1650, 1829, 2466
BstYI	RGATCY	4 cut(s)	913, 1650, 1829, 2466
Bsu36I	CCTNAGG	1 cut(s)	2364
BsuI	GTATCC	2 cut(s)	1186, 2422
BsuRI	GGCC	4 cut(s)	311, 629, 977, 2571
BtgI	CCRYGG	5 cut(s)	97, 837, 899, 1699, 2227
BtsCI	GGATG	4 cut(s)	1320, 1590, 1737, 2176
BtsIMutI	CAGTG	6 cut(s)	582, 1497, 1552, 1800, 1833, 2404
BveI	ACCTGC	1 cut(s)	2226
Cac8I	GCNNGC	8 cut(s)	361, 409, 1168, 1404, 1572, 1638, 2280, 2629
CaiI	CAGNNNCTG	4 cut(s)	788, 931, 1435, 2156
CciI	TCATGA	1 cut(s)	2098
CfoI	GCGC	4 cut(s)	135, 648, 1331, 1733
Cfr10I	RCCGGY	2 cut(s)	1007, 1166
Cfr13I	GGNCC	2 cut(s)	975, 1438
Cfr42I	CCGCGG	1 cut(s)	2230
CseI	GACGC	4 cut(s)	1186, 1760, 1916, 2284
Csp6I	GTAC	5 cut(s)	664, 750, 861, 889, 1341
CspAI	ACCGGT	1 cut(s)	1007
CspCI	CAANNNNNGTGG	4 cut(s)	432, 434, 467, 469
CviQI	GTAC	5 cut(s)	664, 750, 861, 889, 1341
DdeI	CTNAG	7 cut(s)	21, 475, 1146, 1755, 2122, 2166, 2364
DrdI	GACNNNNNNGTC	1 cut(s)	1903
DseDI	GACNNNNNNGTC	1 cut(s)	1903
EaeI	YGGCCR	1 cut(s)	627
Eam1104I	CTCTTC	1 cut(s)	182
EarI	CTCTTC	1 cut(s)	182
EciI	GGCGGA	2 cut(s)	365, 2066
Eco130I	CCWWGG	4 cut(s)	97, 595, 899, 971
Eco147I	AGGCCT	1 cut(s)	2571
Eco31I	GGTCTC	2 cut(s)	107, 1889
Eco32I	GATATC	2 cut(s)	906, 2247
Eco47I	GGWCC	1 cut(s)	1438
Eco47III	AGCGCT	2 cut(s)	134, 647
Eco57I	CTGAAG	4 cut(s)	840, 1053, 1581, 1980
Eco81I	CCTNAGG	1 cut(s)	2364
Eco88I	CYCGRG	1 cut(s)	2333
Eco91I	GGTNACC	1 cut(s)	512
EcoO65I	GGTNACC	1 cut(s)	512
EcoRI	GAATTC	1 cut(s)	705
EcoRII	CCWGG	7 cut(s)	199, 345, 382, 1434, 1516, 1593, 2313
EcoRV	GATATC	2 cut(s)	906, 2247
EcoT14I	CCWWGG	4 cut(s)	97, 595, 899, 971
EcoT22I	ATGCAT	2 cut(s)	1043, 1638
ErhI	CCWWGG	4 cut(s)	97, 595, 899, 971
Esp3I	CGTCTC	1 cut(s)	1934
FaqI	GGGAC	7 cut(s)	214, 362, 880, 977, 1087, 1546, 2467
FauI	CCCGC	2 cut(s)	150, 1882
FblI	GTMKAC	1 cut(s)	1371
FokI	GGATG	4 cut(s)	1327, 1577, 1724, 2183
FspBI	CTAG	8 cut(s)	360, 677, 1092, 1253, 1611, 1662, 2372, 2472
GlaI	GCGC	4 cut(s)	134, 647, 1330, 1732
GsuI	CTGGAG	1 cut(s)	1500
HaeII	RGCGCY	2 cut(s)	136, 649
HaeIII	GGCC	4 cut(s)	311, 629, 977, 2571
HapII	CCGG	6 cut(s)	570, 988, 1008, 1107, 1167, 1924
HgaI	GACGC	4 cut(s)	1186, 1760, 1916, 2284
HhaI	GCGC	4 cut(s)	135, 648, 1331, 1733
Hin1I	GRCGYC	2 cut(s)	1178, 1927
Hin6I	GCGC	4 cut(s)	133, 646, 1329, 1731
HinP1I	GCGC	4 cut(s)	133, 646, 1329, 1731
HincII	GTYRAC	1 cut(s)	1030
HindII	GTYRAC	1 cut(s)	1030
HindIII	AAGCTT	2 cut(s)	1118, 1218
HinfI	GANTC	6 cut(s)	13, 659, 956, 1096, 1465, 2388
HpaI	GTTAAC	1 cut(s)	1030
HpaII	CCGG	6 cut(s)	570, 988, 1008, 1107, 1167, 1924
HphI	GGTGA	8 cut(s)	131, 524, 854, 861, 1390, 1442, 2129, 2254
Hpy166II	GTNNAC	8 cut(s)	1030, 1089, 1290, 1372, 1381, 1768, 2209, 2623
Hpy188I	TCNGA	7 cut(s)	246, 790, 1002, 1742, 2203, 2357, 2422
Hpy8I	GTNNAC	8 cut(s)	1030, 1089, 1290, 1372, 1381, 1768, 2209, 2623
Hpy99I	CGWCG	1 cut(s)	115
HpyCH4IV	ACGT	1 cut(s)	670
HpyF3I	CTNAG	7 cut(s)	21, 475, 1146, 1755, 2122, 2166, 2364
HpySE526I	ACGT	1 cut(s)	670
Hsp92I	GRCGYC	2 cut(s)	1178, 1927
HspAI	GCGC	4 cut(s)	133, 646, 1329, 1731
KroI	GCCGGC	1 cut(s)	1166
KroNI	GCCGGC	1 cut(s)	1168
KspAI	GTTAAC	1 cut(s)	1030
KspI	CCGCGG	1 cut(s)	2230
LmnI	GCTCC	4 cut(s)	727, 990, 1228, 1520
LweI	GCATC	5 cut(s)	141, 1002, 1520, 1546, 1954
MaeI	CTAG	8 cut(s)	360, 677, 1092, 1253, 1611, 1662, 2372, 2472
MaeII	ACGT	1 cut(s)	670
MaeIII	GTNAC	6 cut(s)	137, 512, 1317, 2068, 2117, 2605
MboII	GAAGA	8 cut(s)	199, 1166, 1196, 1439, 1631, 1973, 2054, 2099
MflI	RGATCY	4 cut(s)	913, 1650, 1829, 2466
MhlI	GDGCHC	1 cut(s)	2022
MluI	ACGCGT	1 cut(s)	2276
MlyI	GAGTC	3 cut(s)	7, 653, 2382
MmeI	TCCRAC	4 cut(s)	714, 1493, 1606, 2335
Mph1103I	ATGCAT	2 cut(s)	1043, 1638
MroNI	GCCGGC	1 cut(s)	1166
MroXI	GAANNNNTTC	3 cut(s)	1066, 2106, 2660
MseI	TTAA	6 cut(s)	795, 882, 1029, 1136, 1359, 1880
MslI	CAYNNNNRTG	3 cut(s)	2267, 2507, 2612
MspA1I	CMGCKG	2 cut(s)	20, 2229
MspI	CCGG	6 cut(s)	570, 988, 1008, 1107, 1167, 1924
MspR9I	CCNGG	8 cut(s)	201, 347, 384, 570, 1436, 1518, 1595, 2315
MvaI	CCWGG	7 cut(s)	201, 347, 384, 1436, 1518, 1595, 2315
MvnI	CGCG	4 cut(s)	1329, 1949, 2229, 2278
NaeI	GCCGGC	1 cut(s)	1168
NciI	CCSGG	1 cut(s)	570
NcoI	CCATGG	2 cut(s)	97, 899
NgoMIV	GCCGGC	1 cut(s)	1166
NheI	GCTAGC	1 cut(s)	359
NlaIV	GGNNCC	4 cut(s)	1021, 1425, 1831, 2346
NmuCI	GTSAC	4 cut(s)	137, 512, 2117, 2605
NsiI	ATGCAT	2 cut(s)	1043, 1638
NspI	RCATGY	4 cut(s)	411, 685, 1574, 1640
PaeI	GCATGC	3 cut(s)	411, 1574, 1640
PagI	TCATGA	1 cut(s)	2098
PceI	AGGCCT	1 cut(s)	2571
PciI	ACATGT	1 cut(s)	681
PcsI	WCGNNNNNNNCGW	1 cut(s)	2631
PdiI	GCCGGC	1 cut(s)	1168
PdmI	GAANNNNTTC	3 cut(s)	1066, 2106, 2660
PfeI	GAWTC	3 cut(s)	956, 1096, 1465
PflFI	GACNNNGTC	1 cut(s)	584
PfoI	TCCNGGA	1 cut(s)	1516
PinAI	ACCGGT	1 cut(s)	1007
PleI	GAGTC	3 cut(s)	7, 653, 2382
PpsI	GAGTC	3 cut(s)	7, 653, 2382
PscI	ACATGT	1 cut(s)	681
PsiI	TTATAA	3 cut(s)	161, 2445, 2709
Psp6I	CCWGG	7 cut(s)	199, 345, 382, 1434, 1516, 1593, 2313
PspEI	GGTNACC	1 cut(s)	512
PspGI	CCWGG	7 cut(s)	199, 345, 382, 1434, 1516, 1593, 2313
PspN4I	GGNNCC	4 cut(s)	1021, 1425, 1831, 2346
PspPI	GGNCC	2 cut(s)	975, 1438
PstI	CTGCAG	4 cut(s)	913, 1648, 2152, 2237
PstNI	CAGNNNCTG	4 cut(s)	788, 931, 1435, 2156
PsuI	RGATCY	4 cut(s)	913, 1650, 1829, 2466
PsyI	GACNNNGTC	1 cut(s)	584
PvuII	CAGCTG	1 cut(s)	20
RsaI	GTAC	5 cut(s)	665, 751, 862, 890, 1342
RsaNI	GTAC	5 cut(s)	664, 750, 861, 889, 1341
RseI	CAYNNNNRTG	3 cut(s)	2267, 2507, 2612
SacII	CCGCGG	1 cut(s)	2230
SaqAI	TTAA	6 cut(s)	795, 882, 1029, 1136, 1359, 1880
Sau96I	GGNCC	2 cut(s)	975, 1438
SchI	GAGTC	3 cut(s)	7, 653, 2382
ScrFI	CCNGG	8 cut(s)	201, 347, 384, 570, 1436, 1518, 1595, 2315
SduI	GDGCHC	1 cut(s)	2022
SfaNI	GCATC	5 cut(s)	141, 1002, 1520, 1546, 1954
SfcI	CTRYAG	6 cut(s)	909, 1368, 1644, 2148, 2233, 2521
Sfr303I	CCGCGG	1 cut(s)	2230
SgrBI	CCGCGG	1 cut(s)	2230
SinI	GGWCC	1 cut(s)	1438
SmiMI	CAYNNNNRTG	3 cut(s)	2267, 2507, 2612
SmlI	CTYRAG	4 cut(s)	128, 1931, 2182, 2437
SmoI	CTYRAG	4 cut(s)	128, 1931, 2182, 2437
SphI	GCATGC	3 cut(s)	411, 1574, 1640
SseBI	AGGCCT	1 cut(s)	2571
SspMI	CTAG	8 cut(s)	360, 677, 1092, 1253, 1611, 1662, 2372, 2472
StuI	AGGCCT	1 cut(s)	2571
StyD4I	CCNGG	8 cut(s)	199, 345, 382, 568, 1434, 1516, 1593, 2313
StyI	CCWWGG	4 cut(s)	97, 595, 899, 971
TaiI	ACGT	1 cut(s)	673
TaqI	TCGA	5 cut(s)	118, 210, 723, 831, 2588
TatI	WGTACW	1 cut(s)	888
TauI	GCSGC	4 cut(s)	632, 1329, 2232, 2297
TfiI	GAWTC	3 cut(s)	956, 1096, 1465
Tru1I	TTAA	6 cut(s)	795, 882, 1029, 1136, 1359, 1880
Tru9I	TTAA	6 cut(s)	795, 882, 1029, 1136, 1359, 1880
TscAI	CASTG	6 cut(s)	589, 1504, 1552, 1800, 1840, 2404
TseFI	GTSAC	4 cut(s)	137, 512, 2117, 2605
Tsp45I	GTSAC	4 cut(s)	137, 512, 2117, 2605
TspDTI	ATGAA	8 cut(s)	329, 948, 1214, 1818, 1891, 2087, 2115, 2187
TspGWI	ACGGA	3 cut(s)	364, 529, 1842
TspRI	CASTG	6 cut(s)	589, 1504, 1552, 1800, 1840, 2404
Tth111I	GACNNNGTC	1 cut(s)	584
VpaK11BI	GGWCC	1 cut(s)	1438
XapI	RAATTY	7 cut(s)	705, 1130, 1248, 2102, 2583, 2720, 2734
XbaI	TCTAGA	2 cut(s)	676, 1252
XceI	RCATGY	4 cut(s)	411, 685, 1574, 1640
XcmI	CCANNNNNNNNNTGG	1 cut(s)	450
XmiI	GTMKAC	1 cut(s)	1371
XmnI	GAANNNNTTC	3 cut(s)	1066, 2106, 2660
XspI	CTAG	8 cut(s)	360, 677, 1092, 1253, 1611, 1662, 2372, 2472
Zsp2I	ATGCAT	2 cut(s)	1043, 1638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.