pycom16g15940

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003450
Physical Location & Seq
Forward (+)
11866551 .. 11868471
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGGTGACCTGCTCTGGTGTTCTAAGTTGTTTGTTGAGATTTAAACAATGTACTTTGCATTATAGTTTTCTTCTGCCAATTGCACAGATATTTGGGAGCATCGGCTCCGTGGGAGGTGGTCTAGCTTTAGGCAAAGAGGGTCCTCTTGTTCATACTGGTGCTTGTATTGCTTCTTTGCTTGGACAACTTCTGGAGTGTGCAGGCAATTGTTTGCACATTCTTGTTACTATTATTTTCGTATCATTTTTAAAGTATATCCTAATGATCAGGCGAGCCTTATTTAACCAGCTTACATTTTATATTGCGTACTGGCGCCGAAATTATTTACACAAGAAAGGGAATCGAGTAAAGGTGGGTATTTTACTGTAG

Protein Analysis

123

Amino Acids

13.49

Weight (kDa)

9.74

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 21 - 63 6.9e-08 Voltage gated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 312
AcyI GRCGYC 1 cut(s) 313
AfaI GTAC 2 cut(s) 52, 308
AluBI AGCT 2 cut(s) 125, 289
AluI AGCT 2 cut(s) 125, 289
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 312
BclI TGATCA 1 cut(s) 264
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 365
BfoI RGCGCY 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 106, 141, 314
BmsI GCATC 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 1 cut(s) 108
Bse1I ACTGG 2 cut(s) 160, 314
BseDI CCNNGG 1 cut(s) 108
BseNI ACTGG 2 cut(s) 160, 314
BsgI GTGCAG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 312
Bsp143I GATC 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 106, 141, 314
BspMI ACCTGC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 312
BsrI ACTGG 2 cut(s) 160, 314
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 1 cut(s) 264
BssNI GRCGYC 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 313
BstC8I GCNNGC 2 cut(s) 202, 273
BstDEI CTNAG 1 cut(s) 23
BstDSI CCRYGG 1 cut(s) 108
BstEII GGTNACC 1 cut(s) 4
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 1 cut(s) 267
BstMBI GATC 1 cut(s) 264
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstPI GGTNACC 1 cut(s) 4
BstSFI CTRYAG 1 cut(s) 365
BtgI CCRYGG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 202, 273
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 51, 307
CviJI RGCY 4 cut(s) 105, 125, 275, 289
CviKI_1 RGCY 4 cut(s) 105, 125, 275, 289
CviQI GTAC 2 cut(s) 51, 307
DdeI CTNAG 1 cut(s) 23
DinI GGCGCC 1 cut(s) 314
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
DraI TTTAAA 2 cut(s) 43, 249
Eco47I GGWCC 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 4
EcoO109I RGGNCCY 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 4
EgeI GGCGCC 1 cut(s) 314
EheI GGCGCC 1 cut(s) 314
FaiI YATR 4 cut(s) 63, 153, 255, 300
FbaI TGATCA 1 cut(s) 264
FspBI CTAG 1 cut(s) 122
GlaI GCGC 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 212
HaeII RGCGCY 1 cut(s) 316
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 313
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HinfI GANTC 1 cut(s) 340
HphI GGTGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 4 cut(s) 58, 83, 200, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 313
HspAI GCGC 1 cut(s) 313
KasI GGCGCC 1 cut(s) 312
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 96, 110
LpnPI CCDG 7 cut(s) 22, 141, 176, 186, 253, 295, 299
LweI GCATC 1 cut(s) 108
MaeI CTAG 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 4, 223
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MboII GAAGA 1 cut(s) 62
MfeI CAATTG 2 cut(s) 78, 205
MluCI AATT 3 cut(s) 78, 205, 319
Mly113I GGCGCC 1 cut(s) 313
MnlI CCTC 3 cut(s) 107, 130, 153
MseI TTAA 3 cut(s) 42, 248, 282
MslI CAYNNNNRTG 1 cut(s) 156
MunI CAATTG 2 cut(s) 78, 205
MwoI GCNNNNNNNGC 1 cut(s) 167
NarI GGCGCC 1 cut(s) 313
NdeII GATC 1 cut(s) 264
NlaIV GGNNCC 3 cut(s) 106, 141, 314
NmuCI GTSAC 1 cut(s) 4
PfeI GAWTC 1 cut(s) 340
PluTI GGCGCC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 140
PspEI GGTNACC 1 cut(s) 4
PspN4I GGNNCC 3 cut(s) 106, 141, 314
PspPI GGNCC 1 cut(s) 140
PspPPI RGGWCCY 1 cut(s) 140
RsaI GTAC 2 cut(s) 52, 308
RsaNI GTAC 2 cut(s) 51, 307
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 3 cut(s) 42, 248, 282
Sau3AI GATC 1 cut(s) 264
Sau96I GGNCC 1 cut(s) 140
SetI ASST 5 cut(s) 11, 118, 127, 291, 354
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 365
SfoI GGCGCC 1 cut(s) 314
SinI GGWCC 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 156
Sse9I AATT 3 cut(s) 78, 205, 319
SspDI GGCGCC 1 cut(s) 312
SspMI CTAG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 343
TasI AATT 3 cut(s) 78, 205, 319
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 3 cut(s) 42, 248, 282
Tru9I TTAA 3 cut(s) 42, 248, 282
TseFI GTSAC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 140
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.