pycom16g20850

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012345
Physical Location & Seq
Forward (+)
18916602 .. 18920567
3966 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1746 bp
ATGGGTACTTTTCAGAGCTTTAGGAAGGCCTATGGAGCTCTGAAGGACTCTACCAAGGTTGGCCTCATCAAGGTCAACAGCGAATTCAAGGATTTGGATATTGCCACTGTGAAGGCCACCAGTCACGTAGAGTACCCTCCAAAGGAACGTCACGTTCGAAAGATTTTCTCCGCAACATCAGTGACACGTCCACGTGCAGATGTAGCATACTGCATTCATGCGCTGGCCAAGAGATTGTCCAAGACACGGAGTTGGATTGTTGCCATAAAGACGTTGATAGTTATTCATAGAACATTGAGAGAAGGTGATCCTACCTTCAGAGAGGAGCTTTTGAACTATTCGCAGAGAGGGCACATTCTCCAAATTTCCAATTTTAAAGATGACTCAAGTCCCCTTGCTTGGGATTGCTCCGCCTGGGTGAGGACATATGCACTCTTTTTAGAAGAACGGCTGGAGTGTTTTCGAGTTTTGAAATATGACATTGAGGCAGAGCGTTTGACAAAAACGTCGCCAGGATCAACCAAGGTCCATAGTAGAACACGAACACTGGGTGCTGATGAGCTGTTGGAGCAGCTACCTGCACTGCAGCAACTTCTTTTCCGCCTTATGGGTTGCCAGCCCGAGGGGACAGCTTATAACAATTACCTCATACAATATGCCTTGGCTCTGATATTGAAAGAGAGCTTTAAAATATACTGTGCCATCAATGACGGAATTATAAATCTCGTTGACATGTTCTTCGATATGTCAAGATACGATGCAGTTAAAGCCCTCAATATTTACAAAAGAGCTGGCCAACAGGCTGAAGGGCTTGCTGATTTTTATGAATACTGCAAGGGTTTGGATCTTGCTAGAACTTTTCAGTTTCCAACATTGAGACAGCCCCCACCGTCATTTCTTGCAACAATGGAGGAGTATATAAAGGAGGCGCCTCAGTCAGGTTCCGTTAACAGGAGACTGGAATACCAGGCGTCAGACGAGCAGCCTCAGAGACCTGAAGAACCTCCTCCTCCAGAGCCTGAAAAACAAGACGAAAAAGTTGAGGAGCCACTGCCGAAAACAGAGGAAGAACCCCAACCCAAGGAAGAGGAAGTCGAACCTCCTCCTTTGATATCAACTGATGACACTGATCTGCTGGGTTTAAGGGAAATTAATCCAAAAGCTGCAGAAATCGAAGAAAGCAATGCCTTAGCTCTTGCCATAGTTCCACAGGGTAATGAACAAAAACCTGGAGCGTGTTTCAATGATTTTGCTGGGACTTCCGGTTGGGAGCTAGCACTGGTGACCACACCAAGCAACCATACGAGCCAAACGCCTGTCGACAGAAAGTTGGCTGGTGGCTTTGACAAGCTATTACTTGATAGCTTGTATGAAGATGAAGGTGCCAGGAGACAGTTACAACTCCAGAATGCAGGGTATGGTTATGGTGCGACAAGCTTGCAAAATCCATTTGAACAACAAACACAACAGCCCGTACATGACCCATTTGCAATGTCCAACAGCGTAGCACCCCCAACAAATGTGCAAATGGCGCTCATGGCGCAGCAACAACACCAGCACCAACAAATCATGCAGCAGCAACAACAACAATACCAACAACAGCAAAACATGATGGTGGTACCTCACCCGTATTATTCTCAATATTCCCAACCAATGCAGCCCGCGGGCTCTTCAAACCCGTTCGGAGATCCATTCAGCTTCCCTCCGAGTACAACGTCGCATCAGGGAAACCAAAACCTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

582

Amino Acids

65.73

Weight (kDa)

5.41

Isoelectric Point (pI)

57.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 31 - 309 5.9e-92 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25940 AT5G57200
fragaria_vesca FvH4_4g07320
malus_domestica MD13G1242200.v1.1 MD16G1249100.v1.1
prunus_persica Prupe.1G075800_v2.0.a1 Prupe.1G076000_v2.0.a1
pyrus_communis pycom13g21390 pycom16g20850
rosa_chinensis RchiOBHm_Chr4g0399681
rosa_laevigata RLG00000009247
rosa_multiflora Rmu_co8405013.1_g000001
rosa_roxburghii Rroxscaffold_5G00344590
rosa_rugosa Rorug04G0018100
rosa_samantha Rh4AG095900 Rh4BG093400 Rh4CG104500 Rh4DG088500
rosa_wichuraiana Rw4G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 636, 719
AasI GACNNNNNNGTC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 586
Acc65I GGTACC 1 cut(s) 1618
AccB1I GGYRCC 3 cut(s) 928, 1382, 1618
AccI GTMKAC 1 cut(s) 1320
AccII CGCG 1 cut(s) 1664
AciI CCGC 5 cut(s) 171, 411, 601, 1662, 1664
AclWI GGATC 4 cut(s) 302, 523, 852, 1682
AcoI YGGCCR 2 cut(s) 225, 793
AcsI RAATTY 2 cut(s) 83, 363
AcuI CTGAAG 4 cut(s) 62, 301, 825, 1017
AcvI CACGTG 1 cut(s) 194
AcyI GRCGYC 2 cut(s) 929, 971
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 5 cut(s) 7, 134, 1476, 1620, 1711
AflIII ACRYGT 2 cut(s) 185, 732
AgsI TTSAA 7 cut(s) 88, 334, 472, 676, 1243, 1454, 1674
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 5 cut(s) 413, 511, 966, 1228, 1385
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 4 cut(s) 871, 949, 985, 1384
AlwI GGATC 4 cut(s) 302, 523, 852, 1682
AlwNI CAGNNNCTG 1 cut(s) 1019
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 5 cut(s) 27, 61, 114, 225, 793
ApeKI GCWGC 8 cut(s) 571, 586, 982, 1163, 1543, 1573, 1576, 1657
ApoI RAATTY 2 cut(s) 83, 363
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AseI ATTAAT 1 cut(s) 1152
Asp718I GGTACC 1 cut(s) 1618
AspLEI GCGC 4 cut(s) 223, 931, 1534, 1543
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 4 cut(s) 317, 430, 1294, 1616
AsuII TTCGAA 1 cut(s) 157
AsuNHI GCTAGC 1 cut(s) 1273
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 354
BalI TGGCCA 2 cut(s) 227, 795
BanI GGYRCC 3 cut(s) 928, 1382, 1618
BanII GRGCYC 2 cut(s) 40, 1670
BbrPI CACGTG 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 8 cut(s) 583, 598, 994, 1150, 1555, 1585, 1588, 1669
BccI CCATC 2 cut(s) 710, 1606
BceAI ACGGC 1 cut(s) 464
BcgI CGANNNNNNTGC 2 cut(s) 1420, 1454
BciT130I CCWGG 5 cut(s) 415, 513, 968, 1230, 1387
BcoDI GTCTC 4 cut(s) 871, 949, 985, 1384
BfaI CTAG 2 cut(s) 852, 1274
BfmI CTRYAG 2 cut(s) 584, 1164
BfoI RGCGCY 2 cut(s) 932, 1535
BfuAI ACCTGC 1 cut(s) 586
BisI GCNGC 8 cut(s) 572, 587, 983, 1164, 1544, 1574, 1577, 1658
BlsI GCNGC 8 cut(s) 573, 588, 984, 1165, 1545, 1575, 1578, 1659
Bme1390I CCNGG 5 cut(s) 415, 513, 968, 1230, 1387
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 620
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 5 cut(s) 930, 943, 1047, 1384, 1620
BmrFI CCNGG 5 cut(s) 415, 513, 968, 1230, 1387
BmrI ACTGGG 1 cut(s) 557
BmsI GCATC 2 cut(s) 748, 1729
BmtI GCTAGC 1 cut(s) 1277
BmuI ACTGGG 1 cut(s) 557
BoxI GACNNNNGTC 1 cut(s) 387
BpmI CTGGAG 4 cut(s) 473, 996, 1251, 1388
Bpu10I CCTNAGC 1 cut(s) 1189
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 370
BsaAI YACGTR 2 cut(s) 127, 194
BsaHI GRCGYC 2 cut(s) 929, 971
BsaI GGTCTC 1 cut(s) 985
BsaJI CCNNGG 7 cut(s) 54, 414, 522, 621, 660, 1080, 1662
BsaWI WCCGGW 1 cut(s) 1262
Bse1I ACTGG 4 cut(s) 120, 552, 963, 1284
Bse3DI GCAATG 2 cut(s) 1189, 1497
BseBI CCWGG 5 cut(s) 415, 513, 968, 1230, 1387
BseDI CCNNGG 7 cut(s) 54, 414, 522, 621, 660, 1080, 1662
BseMI GCAATG 2 cut(s) 1189, 1497
BseMII CTCAG 2 cut(s) 947, 1001
BseNI ACTGG 4 cut(s) 120, 552, 963, 1284
BseRI GAGGAG 6 cut(s) 338, 926, 996, 999, 1058, 1092
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 8 cut(s) 583, 598, 994, 1150, 1555, 1585, 1588, 1669
BseYI CCCAGC 2 cut(s) 1135, 1253
BsgI GTGCAG 2 cut(s) 216, 564
Bsh1236I CGCG 1 cut(s) 1664
BshFI GGCC 5 cut(s) 29, 63, 116, 227, 795
BshNI GGYRCC 3 cut(s) 928, 1382, 1618
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 1263
BslFI GGGAC 3 cut(s) 375, 640, 1270
BsmAI GTCTC 4 cut(s) 871, 949, 985, 1384
BsmFI GGGAC 3 cut(s) 375, 640, 1270
BsmI GAATGC 2 cut(s) 213, 1414
BsnI GGCC 5 cut(s) 29, 63, 116, 227, 795
Bso31I GGTCTC 1 cut(s) 985
BsoBI CYCGRG 1 cut(s) 620
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 3 cut(s) 40, 354, 1670
Bsp143I GATC 5 cut(s) 307, 515, 844, 1129, 1687
BspACI CCGC 5 cut(s) 171, 411, 601, 1662, 1664
BspANI GGCC 5 cut(s) 29, 63, 116, 227, 795
BspCNI CTCAG 2 cut(s) 946, 1000
BspFNI CGCG 1 cut(s) 1664
BspLI GGNNCC 5 cut(s) 930, 943, 1047, 1384, 1620
BspMAI CTGCAG 2 cut(s) 588, 1168
BspMI ACCTGC 1 cut(s) 586
BspOI GCTAGC 1 cut(s) 1277
BspPI GGATC 4 cut(s) 302, 523, 852, 1682
BspQI GCTCTTC 1 cut(s) 1675
BspT104I TTCGAA 1 cut(s) 157
BspT107I GGYRCC 3 cut(s) 928, 1382, 1618
BspTNI GGTCTC 1 cut(s) 985
BsrDI GCAATG 2 cut(s) 1189, 1497
BsrI ACTGG 4 cut(s) 120, 552, 963, 1284
BssECI CCNNGG 7 cut(s) 54, 414, 522, 621, 660, 1080, 1662
BssMI GATC 5 cut(s) 307, 515, 844, 1129, 1687
BssNI GRCGYC 2 cut(s) 929, 971
BssT1I CCWWGG 4 cut(s) 54, 522, 660, 1080
Bst2UI CCWGG 5 cut(s) 415, 513, 968, 1230, 1387
Bst4CI ACNGT 4 cut(s) 109, 698, 891, 1395
Bst6I CTCTTC 2 cut(s) 1080, 1675
BstACI GRCGYC 2 cut(s) 929, 971
BstBAI YACGTR 2 cut(s) 127, 194
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 8 cut(s) 225, 617, 793, 813, 1275, 1439, 1662, 1666
BstDEI CTNAG 3 cut(s) 933, 987, 1189
BstDSI CCRYGG 1 cut(s) 1662
BstEII GGTNACC 1 cut(s) 1282
BstENI CCTNNNNNAGG 3 cut(s) 68, 418, 936
BstFNI CGCG 1 cut(s) 1664
BstH2I RGCGCY 2 cut(s) 932, 1535
BstHHI GCGC 4 cut(s) 223, 931, 1534, 1543
BstKTI GATC 5 cut(s) 310, 518, 847, 1132, 1690
BstMAI GTCTC 4 cut(s) 871, 949, 985, 1384
BstMBI GATC 5 cut(s) 307, 515, 844, 1129, 1687
BstMWI GCNNNNNNNGC 8 cut(s) 35, 203, 349, 568, 767, 1531, 1538, 1540
BstNI CCWGG 5 cut(s) 415, 513, 968, 1230, 1387
BstNSI RCATGY 1 cut(s) 736
BstPAI GACNNNNGTC 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 1282
BstSCI CCNGG 5 cut(s) 413, 511, 966, 1228, 1385
BstSFI CTRYAG 2 cut(s) 584, 1164
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 1664
BstV1I GCAGC 8 cut(s) 583, 598, 994, 1150, 1555, 1585, 1588, 1669
BstX2I RGATCY 2 cut(s) 844, 1687
BstYI RGATCY 2 cut(s) 844, 1687
BsuRI GGCC 5 cut(s) 29, 63, 116, 227, 795
BtgI CCRYGG 1 cut(s) 1662
BtrI CACGTC 1 cut(s) 188
BtsI GCAGTG 2 cut(s) 581, 1049
BtsIMutI CAGTG 7 cut(s) 105, 186, 545, 581, 1049, 1125, 1277
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 8 cut(s) 225, 617, 793, 813, 1275, 1439, 1662, 1666
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 4 cut(s) 223, 931, 1534, 1543
Cfr13I GGNCC 1 cut(s) 526
Cfr42I CCGCGG 1 cut(s) 1665
CseI GACGC 1 cut(s) 960
Csp6I GTAC 5 cut(s) 6, 133, 1475, 1619, 1710
CviAII CATG 6 cut(s) 218, 733, 1478, 1537, 1570, 1609
CviQI GTAC 5 cut(s) 6, 133, 1475, 1619, 1710
DdeI CTNAG 3 cut(s) 933, 987, 1189
DinI GGCGCC 1 cut(s) 930
DpnI GATC 5 cut(s) 309, 517, 846, 1131, 1689
DpnII GATC 5 cut(s) 307, 515, 844, 1129, 1687
DraI TTTAAA 2 cut(s) 376, 688
DraIII CACNNNGTG 1 cut(s) 551
DrdI GACNNNNNNGTC 1 cut(s) 505
DseDI GACNNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 2 cut(s) 225, 793
Eam1104I CTCTTC 2 cut(s) 1080, 1675
EarI CTCTTC 2 cut(s) 1080, 1675
EciI GGCGGA 2 cut(s) 400, 590
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 4 cut(s) 54, 522, 660, 1080
Eco147I AGGCCT 1 cut(s) 29
Eco24I GRGCYC 2 cut(s) 40, 1670
Eco31I GGTCTC 1 cut(s) 985
Eco32I GATATC 1 cut(s) 1113
Eco47I GGWCC 1 cut(s) 526
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 4 cut(s) 62, 301, 825, 1017
Eco72I CACGTG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 620
Eco91I GGTNACC 1 cut(s) 1282
EcoICRI GAGCTC 1 cut(s) 38
EcoNI CCTNNNNNAGG 3 cut(s) 68, 418, 936
EcoO65I GGTNACC 1 cut(s) 1282
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 5 cut(s) 413, 511, 966, 1228, 1385
EcoRV GATATC 1 cut(s) 1113
EcoT14I CCWWGG 4 cut(s) 54, 522, 660, 1080
EcoT38I GRGCYC 2 cut(s) 40, 1670
EgeI GGCGCC 1 cut(s) 930
EheI GGCGCC 1 cut(s) 930
ErhI CCWWGG 4 cut(s) 54, 522, 660, 1080
FaeI CATG 6 cut(s) 221, 736, 1481, 1540, 1573, 1612
FaqI GGGAC 3 cut(s) 375, 640, 1270
FatI CATG 6 cut(s) 217, 732, 1477, 1536, 1569, 1608
FauI CCCGC 2 cut(s) 1657, 1669
FauNDI CATATG 1 cut(s) 427
FblI GTMKAC 1 cut(s) 1320
Fnu4HI GCNGC 8 cut(s) 572, 587, 983, 1164, 1544, 1574, 1577, 1658
FriOI GRGCYC 2 cut(s) 40, 1670
Fsp4HI GCNGC 8 cut(s) 572, 587, 983, 1164, 1544, 1574, 1577, 1658
FspBI CTAG 2 cut(s) 852, 1274
GlaI GCGC 4 cut(s) 222, 930, 1533, 1542
GluI GCNGC 8 cut(s) 572, 587, 983, 1164, 1544, 1574, 1577, 1658
GsaI CCCAGC 2 cut(s) 1139, 1257
GsuI CTGGAG 4 cut(s) 473, 996, 1251, 1388
HaeII RGCGCY 2 cut(s) 932, 1535
HaeIII GGCC 5 cut(s) 29, 63, 116, 227, 795
HapII CCGG 1 cut(s) 1263
HgaI GACGC 1 cut(s) 960
HhaI GCGC 4 cut(s) 223, 931, 1534, 1543
Hin1I GRCGYC 2 cut(s) 929, 971
Hin1II CATG 6 cut(s) 221, 736, 1481, 1540, 1573, 1612
Hin6I GCGC 4 cut(s) 221, 929, 1532, 1541
HinP1I GCGC 4 cut(s) 221, 929, 1532, 1541
HincII GTYRAC 4 cut(s) 76, 730, 949, 1321
HindII GTYRAC 4 cut(s) 76, 730, 949, 1321
HindIII AAGCTT 1 cut(s) 1435
HinfI GANTC 2 cut(s) 47, 383
HpaI GTTAAC 1 cut(s) 949
HpaII CCGG 1 cut(s) 1263
HphI GGTGA 4 cut(s) 317, 430, 1294, 1616
Hpy166II GTNNAC 5 cut(s) 76, 191, 730, 949, 1321
Hpy188I TCNGA 8 cut(s) 15, 42, 320, 669, 976, 990, 1685, 1707
Hpy188III TCNNGA 3 cut(s) 750, 1013, 1405
Hpy8I GTNNAC 5 cut(s) 76, 191, 730, 949, 1321
Hpy99I CGWCG 2 cut(s) 511, 1720
HpyAV CCTTC 7 cut(s) 19, 37, 106, 296, 325, 800, 1373
HpyCH4III ACNGT 4 cut(s) 109, 698, 891, 1395
HpyCH4IV ACGT 8 cut(s) 126, 148, 153, 187, 193, 272, 506, 1715
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 203, 349, 568, 767, 1531, 1538, 1540
HpyF3I CTNAG 3 cut(s) 933, 987, 1189
HpySE526I ACGT 8 cut(s) 126, 148, 153, 187, 193, 272, 506, 1715
Hsp92I GRCGYC 2 cut(s) 929, 971
Hsp92II CATG 6 cut(s) 221, 736, 1481, 1540, 1573, 1612
HspAI GCGC 4 cut(s) 221, 929, 1532, 1541
KasI GGCGCC 1 cut(s) 928
KpnI GGTACC 1 cut(s) 1622
KspAI GTTAAC 1 cut(s) 949
KspI CCGCGG 1 cut(s) 1665
Kzo9I GATC 5 cut(s) 307, 515, 844, 1129, 1687
LguI GCTCTTC 1 cut(s) 1675
LmnI GCTCC 7 cut(s) 35, 325, 413, 568, 1045, 1232, 1270
Lsp1109I GCAGC 8 cut(s) 583, 598, 994, 1150, 1555, 1585, 1588, 1669
LweI GCATC 2 cut(s) 748, 1729
MaeI CTAG 2 cut(s) 852, 1274
MaeII ACGT 8 cut(s) 126, 148, 153, 187, 193, 272, 506, 1715
MaeIII GTNAC 5 cut(s) 122, 149, 181, 1282, 1395
MalI GATC 5 cut(s) 309, 517, 846, 1131, 1689
MboI GATC 5 cut(s) 307, 515, 844, 1129, 1687
MboII GAAGA 8 cut(s) 455, 730, 1010, 1079, 1097, 1187, 1385, 1662
MflI RGATCY 2 cut(s) 844, 1687
MhlI GDGCHC 3 cut(s) 40, 354, 1670
MlsI TGGCCA 2 cut(s) 227, 795
MluCI AATT 6 cut(s) 83, 363, 370, 640, 714, 1149
MluNI TGGCCA 2 cut(s) 227, 795
Mly113I GGCGCC 1 cut(s) 929
MlyI GAGTC 2 cut(s) 41, 377
MmeI TCCRAC 4 cut(s) 233, 546, 893, 1521
Mox20I TGGCCA 2 cut(s) 227, 795
MscI TGGCCA 2 cut(s) 227, 795
MseI TTAA 6 cut(s) 375, 687, 765, 948, 1142, 1152
MslI CAYNNNNRTG 1 cut(s) 1613
Msp20I TGGCCA 2 cut(s) 227, 795
MspA1I CMGCKG 1 cut(s) 1664
MspI CCGG 1 cut(s) 1263
MspR9I CCNGG 5 cut(s) 415, 513, 968, 1230, 1387
Mva1269I GAATGC 2 cut(s) 213, 1414
MvaI CCWGG 5 cut(s) 415, 513, 968, 1230, 1387
MvnI CGCG 1 cut(s) 1664
MwoI GCNNNNNNNGC 8 cut(s) 35, 203, 349, 568, 767, 1531, 1538, 1540
NarI GGCGCC 1 cut(s) 929
NdeI CATATG 1 cut(s) 427
NdeII GATC 5 cut(s) 307, 515, 844, 1129, 1687
NheI GCTAGC 1 cut(s) 1273
NlaIII CATG 6 cut(s) 221, 736, 1481, 1540, 1573, 1612
NlaIV GGNNCC 5 cut(s) 930, 943, 1047, 1384, 1620
NmuCI GTSAC 4 cut(s) 122, 149, 181, 1282
NspI RCATGY 1 cut(s) 736
NspV TTCGAA 1 cut(s) 157
PceI AGGCCT 1 cut(s) 29
PciI ACATGT 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 1675
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 2 cut(s) 213, 1414
PkrI GCNGC 8 cut(s) 573, 588, 984, 1165, 1545, 1575, 1578, 1659
PleI GAGTC 2 cut(s) 41, 377
PluTI GGCGCC 1 cut(s) 932
PmaCI CACGTG 1 cut(s) 194
PmlI CACGTG 1 cut(s) 194
PpsI GAGTC 2 cut(s) 41, 377
Ppu21I YACGTR 2 cut(s) 127, 194
PscI ACATGT 1 cut(s) 732
PshAI GACNNNNGTC 1 cut(s) 387
PshBI ATTAAT 1 cut(s) 1152
PsiI TTATAA 2 cut(s) 636, 719
Psp124BI GAGCTC 1 cut(s) 40
Psp6I CCWGG 5 cut(s) 413, 511, 966, 1228, 1385
PspCI CACGTG 1 cut(s) 194
PspEI GGTNACC 1 cut(s) 1282
PspFI CCCAGC 2 cut(s) 1135, 1253
PspGI CCWGG 5 cut(s) 413, 511, 966, 1228, 1385
PspN4I GGNNCC 5 cut(s) 930, 943, 1047, 1384, 1620
PspPI GGNCC 1 cut(s) 526
PstI CTGCAG 2 cut(s) 588, 1168
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 2 cut(s) 844, 1687
RsaI GTAC 5 cut(s) 7, 134, 1476, 1620, 1711
RsaNI GTAC 5 cut(s) 6, 133, 1475, 1619, 1710
RseI CAYNNNNRTG 1 cut(s) 1613
SacI GAGCTC 1 cut(s) 40
SacII CCGCGG 1 cut(s) 1665
SalI GTCGAC 1 cut(s) 1319
SapI GCTCTTC 1 cut(s) 1675
SaqAI TTAA 6 cut(s) 375, 687, 765, 948, 1142, 1152
SatI GCNGC 8 cut(s) 572, 587, 983, 1164, 1544, 1574, 1577, 1658
Sau3AI GATC 5 cut(s) 307, 515, 844, 1129, 1687
Sau96I GGNCC 1 cut(s) 526
SchI GAGTC 2 cut(s) 41, 377
ScrFI CCNGG 5 cut(s) 415, 513, 968, 1230, 1387
SduI GDGCHC 3 cut(s) 40, 354, 1670
SfaNI GCATC 2 cut(s) 748, 1729
SfcI CTRYAG 2 cut(s) 584, 1164
SfoI GGCGCC 1 cut(s) 930
Sfr303I CCGCGG 1 cut(s) 1665
SfuI TTCGAA 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 1665
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 1613
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 6 cut(s) 83, 363, 370, 640, 714, 1149
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 5 cut(s) 171, 411, 601, 1662, 1664
SspDI GGCGCC 1 cut(s) 928
SspI AATATT 2 cut(s) 778, 1643
SspMI CTAG 2 cut(s) 852, 1274
SstI GAGCTC 1 cut(s) 40
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 5 cut(s) 413, 511, 966, 1228, 1385
StyI CCWWGG 4 cut(s) 54, 522, 660, 1080
TaaI ACNGT 4 cut(s) 109, 698, 891, 1395
TaiI ACGT 8 cut(s) 129, 151, 156, 190, 196, 275, 509, 1718
TaqI TCGA 6 cut(s) 157, 463, 741, 1095, 1173, 1320
TasI AATT 6 cut(s) 83, 363, 370, 640, 714, 1149
TatI WGTACW 1 cut(s) 1709
Tru1I TTAA 6 cut(s) 375, 687, 765, 948, 1142, 1152
Tru9I TTAA 6 cut(s) 375, 687, 765, 948, 1142, 1152
TscAI CASTG 7 cut(s) 112, 186, 552, 588, 1056, 1132, 1284
TseFI GTSAC 4 cut(s) 122, 149, 181, 1282
TseI GCWGC 8 cut(s) 571, 586, 982, 1163, 1543, 1573, 1576, 1657
Tsp45I GTSAC 4 cut(s) 122, 149, 181, 1282
TspDTI ATGAA 6 cut(s) 206, 275, 840, 1233, 1386, 1392
TspGWI ACGGA 3 cut(s) 262, 726, 934
TspRI CASTG 7 cut(s) 112, 186, 552, 588, 1056, 1132, 1284
VpaK11BI GGWCC 1 cut(s) 526
VspI ATTAAT 1 cut(s) 1152
XagI CCTNNNNNAGG 3 cut(s) 68, 418, 936
XapI RAATTY 2 cut(s) 83, 363
XceI RCATGY 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 1320
XspI CTAG 2 cut(s) 852, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.