pycom16g21750

U4 U6.U5 small nuclear ribonucleoprotein 27 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010002
Physical Location & Seq
Forward (+)
20508738 .. 20509460
723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCCAAAATCCAAACTCGCACCGGAATCAACTTGGTTTTACTTGATAAAAGCACTTCTCCCAAAATCACGAACCACCTCCCAGTTACTCCTTACTCTCACGCGGACTATGGGGGACCGAGAACGAGACAGGGACCGGGATCGTGACCGAATTCGGGACAGAGACCGTGACCGAGAAAGACGCCGAGAGCGAGACGACCGCGACCGTGACCGCGTCCGCATCAAGAGGTCGCGTACACCCGAACGCACCAGACCACGCCACACCCGCTCCCGCACGCGCTCGCCCGACCGGTACCGCTCCCGCACTCGCTCCATCTCCCGTTCTCGGTCTCCAGACGACAGGTCCCACCGGCGGCGCCAGCACCACCACCGCAGCCCCTCGCCAGAGCCACGCAAGCGCCAGCGGCGTGAGTCGACTGTAGAGGACGAGAGGGAGCGGCAGAAGGCGGTGGTTTTGGACTCCGTTGAGGGCATCTCGAAGGAGCCGAAAACTCTGCAGAAGCCGAGCAGCGATGGTGGTGGTGGTGGTGAGGGTATGGACGAGGACGAGATTGAGATGATGCAGAAATTGGGGATTCCGATTGGGTTTGACTCGACAAAAGGGAAGCCAGTGGCGGGCGCAGATGTGAGTGGCGTTAGGGCGGTCACTAAGCGGCAGCCGAGACAGTATATGAATCGCCGCGGTGGATTTAATCGGCCGTTGCCTGCCGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

241

Amino Acids

28.37

Weight (kDa)

11.4

Isoelectric Point (pI)

78.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 181 - 236 4.1e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 291
AccB1I GGYRCC 2 cut(s) 291, 354
AccBSI CCGCTC 3 cut(s) 267, 297, 436
AccI GTMKAC 1 cut(s) 413
AccII CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
AclWI GGATC 1 cut(s) 147
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 1 cut(s) 150
AcyI GRCGYC 2 cut(s) 181, 355
AfaI GTAC 2 cut(s) 235, 293
AfiI CCNNNNNNNGG 4 cut(s) 154, 324, 351, 614
AgeI ACCGGT 1 cut(s) 288
AhdI GACNNNNNGTC 1 cut(s) 340
Alw26I GTCTC 5 cut(s) 120, 156, 186, 333, 655
AlwI GGATC 1 cut(s) 147
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 372, 507, 655
ApoI RAATTY 1 cut(s) 150
AsiGI ACCGGT 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 291
AspLEI GCGC 5 cut(s) 279, 357, 399, 620, 714
AspS9I GGNCC 3 cut(s) 115, 133, 342
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 3 cut(s) 115, 133, 342
BanI GGYRCC 2 cut(s) 291, 354
BbvI GCAGC 3 cut(s) 384, 519, 667
BccI CCATC 2 cut(s) 320, 506
BceAI ACGGC 1 cut(s) 682
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 5 cut(s) 120, 156, 186, 333, 655
BfaI CTAG 1 cut(s) 721
BfmI CTRYAG 2 cut(s) 417, 494
BfoI RGCGCY 2 cut(s) 358, 400
BisI GCNGC 8 cut(s) 353, 373, 404, 437, 508, 653, 656, 679
BlsI GCNGC 8 cut(s) 354, 374, 405, 438, 509, 654, 657, 680
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 3 cut(s) 115, 133, 342
BmeRI GACNNNNNGTC 1 cut(s) 340
BmgT120I GGNCC 3 cut(s) 115, 133, 342
BmiI GGNNCC 6 cut(s) 116, 134, 293, 344, 356, 483
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 228, 480, 549
BmuI ACTGGG 1 cut(s) 76
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 137
BsaHI GRCGYC 2 cut(s) 181, 355
BsaI GGTCTC 2 cut(s) 156, 333
BsaJI CCNNGG 1 cut(s) 679
BsaWI WCCGGW 2 cut(s) 22, 288
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 324, 351, 614
Bse118I RCCGGY 2 cut(s) 288, 348
Bse1I ACTGG 2 cut(s) 82, 608
BseDI CCNNGG 1 cut(s) 679
BseLI CCNNNNNNNGG 4 cut(s) 154, 324, 351, 614
BseNI ACTGG 2 cut(s) 82, 608
BseX3I CGGCCG 1 cut(s) 695
BseXI GCAGC 3 cut(s) 384, 519, 667
Bsh1236I CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
Bsh1285I CGRYCG 4 cut(s) 199, 205, 289, 698
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 2 cut(s) 291, 354
BshTI ACCGGT 1 cut(s) 288
BsiEI CGRYCG 4 cut(s) 199, 205, 289, 698
BsiSI CCGG 4 cut(s) 23, 136, 289, 349
BslFI GGGAC 4 cut(s) 128, 146, 170, 328
BslI CCNNNNNNNGG 4 cut(s) 154, 324, 351, 614
BsmAI GTCTC 5 cut(s) 120, 156, 186, 333, 655
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 4 cut(s) 128, 146, 170, 328
BsnI GGCC 1 cut(s) 697
Bso31I GGTCTC 2 cut(s) 156, 333
Bsp143I GATC 1 cut(s) 139
BspANI GGCC 1 cut(s) 697
BspFNI CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
BspLI GGNNCC 6 cut(s) 116, 134, 293, 344, 356, 483
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 1 cut(s) 147
BspT107I GGYRCC 2 cut(s) 291, 354
BspTNI GGTCTC 2 cut(s) 156, 333
BsrBI CCGCTC 3 cut(s) 267, 297, 436
BsrFI RCCGGY 2 cut(s) 288, 348
BsrI ACTGG 2 cut(s) 82, 608
BssAI RCCGGY 2 cut(s) 288, 348
BssECI CCNNGG 1 cut(s) 679
BssMI GATC 1 cut(s) 139
BssNI GRCGYC 2 cut(s) 181, 355
Bst4CI ACNGT 4 cut(s) 167, 206, 418, 666
BstACI GRCGYC 2 cut(s) 181, 355
BstC8I GCNNGC 7 cut(s) 275, 281, 359, 395, 401, 616, 705
BstDEI CTNAG 1 cut(s) 648
BstDSI CCRYGG 1 cut(s) 679
BstFNI CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
BstH2I RGCGCY 2 cut(s) 358, 400
BstHHI GCGC 5 cut(s) 279, 357, 399, 620, 714
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 5 cut(s) 120, 156, 186, 333, 655
BstMBI GATC 1 cut(s) 139
BstMCI CGRYCG 4 cut(s) 199, 205, 289, 698
BstMWI GCNNNNNNNGC 5 cut(s) 264, 358, 394, 403, 709
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 2 cut(s) 417, 494
BstUI CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
BstV1I GCAGC 3 cut(s) 384, 519, 667
BstZI CGGCCG 1 cut(s) 695
BsuRI GGCC 1 cut(s) 697
BtgI CCRYGG 1 cut(s) 679
BtgZI GCGATG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 615
Cac8I GCNNGC 7 cut(s) 275, 281, 359, 395, 401, 616, 705
CfoI GCGC 5 cut(s) 279, 357, 399, 620, 714
Cfr10I RCCGGY 2 cut(s) 288, 348
Cfr13I GGNCC 3 cut(s) 115, 133, 342
Cfr42I CCGCGG 1 cut(s) 682
CseI GACGC 2 cut(s) 189, 202
Csp6I GTAC 2 cut(s) 234, 292
CspAI ACCGGT 1 cut(s) 288
CviJI RGCY 7 cut(s) 375, 388, 484, 502, 607, 658, 697
CviKI_1 RGCY 7 cut(s) 375, 388, 484, 502, 607, 658, 697
CviQI GTAC 2 cut(s) 234, 292
DdeI CTNAG 1 cut(s) 648
DinI GGCGCC 1 cut(s) 356
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
DriI GACNNNNNGTC 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 695
EagI CGGCCG 1 cut(s) 695
Eam1105I GACNNNNNGTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 695
Eco31I GGTCTC 2 cut(s) 156, 333
Eco47I GGWCC 3 cut(s) 115, 133, 342
Eco52I CGGCCG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 356
EheI GGCGCC 1 cut(s) 356
Esp3I CGTCTC 1 cut(s) 186
FaiI YATR 4 cut(s) 110, 536, 669, 671
FaqI GGGAC 4 cut(s) 128, 146, 170, 328
FauI CCCGC 4 cut(s) 272, 278, 308, 607
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 8 cut(s) 353, 373, 404, 437, 508, 653, 656, 679
Fsp4HI GCNGC 8 cut(s) 353, 373, 404, 437, 508, 653, 656, 679
FspBI CTAG 1 cut(s) 721
GlaI GCGC 5 cut(s) 278, 356, 398, 619, 713
GluI GCNGC 8 cut(s) 353, 373, 404, 437, 508, 653, 656, 679
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 2 cut(s) 358, 400
HaeIII GGCC 1 cut(s) 697
HapII CCGG 4 cut(s) 23, 136, 289, 349
HgaI GACGC 2 cut(s) 189, 202
HhaI GCGC 5 cut(s) 279, 357, 399, 620, 714
Hin1I GRCGYC 2 cut(s) 181, 355
Hin6I GCGC 5 cut(s) 277, 355, 397, 618, 712
HinP1I GCGC 5 cut(s) 277, 355, 397, 618, 712
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 6 cut(s) 26, 410, 458, 574, 590, 673
HpaII CCGG 4 cut(s) 23, 136, 289, 349
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 2 cut(s) 236, 414
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 6 cut(s) 69, 143, 155, 223, 332, 475
Hpy8I GTNNAC 2 cut(s) 236, 414
HpyAV CCTTC 2 cut(s) 436, 472
HpyCH4III ACNGT 4 cut(s) 167, 206, 418, 666
HpyCH4V TGCA 2 cut(s) 496, 562
HpyF10VI GCNNNNNNNGC 5 cut(s) 264, 358, 394, 403, 709
HpyF3I CTNAG 1 cut(s) 648
Hsp92I GRCGYC 2 cut(s) 181, 355
HspAI GCGC 5 cut(s) 277, 355, 397, 618, 712
KasI GGCGCC 1 cut(s) 354
KpnI GGTACC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 682
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 5 cut(s) 272, 302, 314, 433, 481
Lsp1109I GCAGC 3 cut(s) 384, 519, 667
LweI GCATC 3 cut(s) 228, 480, 549
MaeI CTAG 1 cut(s) 721
MaeIII GTNAC 5 cut(s) 84, 143, 167, 206, 643
MalI GATC 1 cut(s) 141
MbiI CCGCTC 3 cut(s) 267, 297, 436
MboI GATC 1 cut(s) 139
MluCI AATT 2 cut(s) 150, 566
Mly113I GGCGCC 1 cut(s) 355
MlyI GAGTC 3 cut(s) 419, 452, 584
MnlI CCTC 8 cut(s) 88, 219, 388, 415, 423, 460, 523, 535
MseI TTAA 1 cut(s) 690
MspA1I CMGCKG 2 cut(s) 403, 681
MspI CCGG 4 cut(s) 23, 136, 289, 349
MspR9I CCNGG 1 cut(s) 137
MvnI CGCG 6 cut(s) 103, 201, 213, 232, 277, 681
MwoI GCNNNNNNNGC 5 cut(s) 264, 358, 394, 403, 709
NarI GGCGCC 1 cut(s) 355
NciI CCSGG 1 cut(s) 137
NdeII GATC 1 cut(s) 139
NlaIV GGNNCC 6 cut(s) 116, 134, 293, 344, 356, 483
NmeAIII GCCGAG 3 cut(s) 209, 528, 684
NmuCI GTSAC 4 cut(s) 143, 167, 206, 643
PcsI WCGNNNNNNNCGW 2 cut(s) 187, 482
PfeI GAWTC 3 cut(s) 26, 574, 673
PflFI GACNNNGTC 1 cut(s) 212
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 8 cut(s) 354, 374, 405, 438, 509, 654, 657, 680
PleI GAGTC 3 cut(s) 418, 452, 584
PluTI GGCGCC 1 cut(s) 358
PpsI GAGTC 3 cut(s) 418, 452, 584
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
PspN4I GGNNCC 6 cut(s) 116, 134, 293, 344, 356, 483
PspPI GGNCC 3 cut(s) 115, 133, 342
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 1 cut(s) 498
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 2 cut(s) 235, 293
RsaNI GTAC 2 cut(s) 234, 292
SacII CCGCGG 1 cut(s) 682
SalI GTCGAC 1 cut(s) 412
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 8 cut(s) 353, 373, 404, 437, 508, 653, 656, 679
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 3 cut(s) 115, 133, 342
SchI GAGTC 3 cut(s) 419, 452, 584
ScrFI CCNGG 1 cut(s) 137
SetI ASST 3 cut(s) 80, 230, 344
SfaNI GCATC 3 cut(s) 228, 480, 549
SfcI CTRYAG 2 cut(s) 417, 494
SfoI GGCGCC 1 cut(s) 356
Sfr303I CCGCGG 1 cut(s) 682
SgrAI CRCCGGYG 1 cut(s) 348
SgrBI CCGCGG 1 cut(s) 682
SinI GGWCC 3 cut(s) 115, 133, 342
Sse9I AATT 2 cut(s) 150, 566
SspDI GGCGCC 1 cut(s) 354
SspMI CTAG 1 cut(s) 721
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 4 cut(s) 167, 206, 418, 666
TaqI TCGA 3 cut(s) 413, 476, 593
TaqII GACCGA 4 cut(s) 132, 162, 186, 315
TasI AATT 2 cut(s) 150, 566
TauI GCSGC 5 cut(s) 355, 406, 439, 655, 681
TfiI GAWTC 3 cut(s) 26, 574, 673
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 1 cut(s) 615
TseFI GTSAC 4 cut(s) 143, 167, 206, 643
TseI GCWGC 3 cut(s) 372, 507, 655
Tsp45I GTSAC 4 cut(s) 143, 167, 206, 643
TspDTI ATGAA 1 cut(s) 686
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 1 cut(s) 615
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 3 cut(s) 115, 133, 342
XapI RAATTY 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.