pycom16g24590

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009435
Physical Location & Seq
Forward (+)
26170904 .. 26172241
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGCTGTCAACCAGAATTAGTGTCATGGACCATGCCATTCCCTGCAATCCCTGCGTTTCCCTTTCTATCCACCGGAAACCGCCACTCTACCGCCAAATCACTTGGCCAAACACCGTCACCCACCACTCCAACATAACCAAATGGCGCCGCAACACGATTTCCGAGCTCCAAACTCCGGTGGTTGTCACCGACCGAACCGGTGACTACTCTCCTTCAGTTCCCACACACAAAGTCACCGTTCACGACAGACAACGAGGAGTCTCCCACCAGTTCCTCGTCCCCGAGGACCAGTACATATTGCACACAGCAGAGTCGCAGGACATCACGCTTCCATTCGCTTGCAGGCACGGTTGTTGTACTAGTTGTGCGGTACGTGTAAAATCTGGAGAACTTAGACAGCCTGAGGCACTAGGAATTTCTACCGAACTGAAATCAAAGGGCTATGCACTTCTCTGTGTGGGTTATCCATCATCTGATCGATTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 map00195
Pfam Domains
Protein Families
Fer2

Protein Analysis

163

Amino Acids

18.2

Weight (kDa)

9.06

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 85 - 156 7e-15 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 144
AciI CCGC 4 cut(s) 80, 91, 148, 368
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 3 cut(s) 293, 358, 372
AfiI CCNNNNNNNGG 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 373
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 484
AhlI ACTAGT 1 cut(s) 359
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 1 cut(s) 104
ApoI RAATTY 1 cut(s) 414
AsiGI ACCGGT 1 cut(s) 197
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 28, 286
AsuHPI GGTGA 4 cut(s) 109, 178, 212, 226
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 28, 286
AxyI CCTNAGG 1 cut(s) 402
BalI TGGCCA 1 cut(s) 106
BanI GGYRCC 1 cut(s) 144
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 265
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 2 cut(s) 360, 410
BfoI RGCGCY 1 cut(s) 148
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme18I GGWCC 2 cut(s) 28, 286
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 28, 286
BmiI GGNNCC 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 478
BsaAI YACGTR 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 282
BsaWI WCCGGW 3 cut(s) 72, 175, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 268, 289
Bse21I CCTNAGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 478
BseDI CCNNGG 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 2 cut(s) 268, 289
BseRI GAGGAG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 144
BshTI ACCGGT 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 478
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 3 cut(s) 73, 176, 198
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 1 cut(s) 475
BspACI CCGC 4 cut(s) 80, 91, 148, 368
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 394
BspDI ATCGAT 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 2 cut(s) 268, 289
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 1 cut(s) 282
BssMI GATC 1 cut(s) 475
BssNI GRCGYC 1 cut(s) 145
Bst4CI ACNGT 3 cut(s) 115, 238, 350
BstACI GRCGYC 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 340, 344
BstDEI CTNAG 2 cut(s) 392, 402
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 478
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 1 cut(s) 475
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 478
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 1 cut(s) 106
BsuTUI ATCGAT 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 340, 344
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 28, 286
ClaI ATCGAT 1 cut(s) 478
Csp6I GTAC 3 cut(s) 292, 357, 371
CspAI ACCGGT 1 cut(s) 197
CviAII CATG 2 cut(s) 25, 32
CviJI RGCY 4 cut(s) 106, 166, 400, 441
CviKI_1 RGCY 4 cut(s) 106, 166, 400, 441
CviQI GTAC 3 cut(s) 292, 357, 371
DdeI CTNAG 2 cut(s) 392, 402
DinI GGCGCC 1 cut(s) 146
DpnI GATC 1 cut(s) 477
DpnII GATC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 28, 286
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 198
Eco81I CCTNAGG 1 cut(s) 402
Eco88I CYCGRG 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 168
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
FaeI CATG 2 cut(s) 28, 35
FaiI YATR 5 cut(s) 26, 33, 134, 296, 444
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 24, 31
Fnu4HI GCNGC 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 2 cut(s) 360, 410
GlaI GCGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 1 cut(s) 106
HapII CCGG 3 cut(s) 73, 176, 198
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 2 cut(s) 28, 35
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 2 cut(s) 258, 311
HpaII CCGG 3 cut(s) 73, 176, 198
HphI GGTGA 4 cut(s) 109, 178, 212, 226
Hpy166II GTNNAC 2 cut(s) 9, 241
Hpy188I TCNGA 2 cut(s) 163, 475
Hpy188III TCNNGA 2 cut(s) 242, 384
Hpy8I GTNNAC 2 cut(s) 9, 241
HpyAV CCTTC 1 cut(s) 222
HpyCH4III ACNGT 3 cut(s) 115, 238, 350
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 4 cut(s) 45, 301, 342, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 2 cut(s) 392, 402
HpySE526I ACGT 1 cut(s) 373
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 2 cut(s) 28, 35
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
Kzo9I GATC 1 cut(s) 475
LmnI GCTCC 1 cut(s) 171
MaeI CTAG 2 cut(s) 360, 410
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 4 cut(s) 115, 184, 200, 232
MalI GATC 1 cut(s) 477
MboI GATC 1 cut(s) 475
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 106
MluCI AATT 2 cut(s) 15, 414
MluNI TGGCCA 1 cut(s) 106
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 2 cut(s) 267, 320
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 248, 277, 284, 397
Mox20I TGGCCA 1 cut(s) 106
MscI TGGCCA 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 106
MspI CCGG 3 cut(s) 73, 176, 198
MwoI GCNNNNNNNGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 145
NdeII GATC 1 cut(s) 475
NlaIII CATG 2 cut(s) 28, 35
NlaIV GGNNCC 1 cut(s) 146
NmuCI GTSAC 4 cut(s) 115, 184, 200, 232
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 2 cut(s) 266, 319
PluTI GGCGCC 1 cut(s) 148
PpsI GAGTC 2 cut(s) 266, 319
Ppu21I YACGTR 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 146
PspPI GGNCC 2 cut(s) 28, 286
RsaI GTAC 3 cut(s) 293, 358, 372
RsaNI GTAC 3 cut(s) 292, 357, 371
SacI GAGCTC 1 cut(s) 168
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 1 cut(s) 475
Sau96I GGNCC 2 cut(s) 28, 286
SchI GAGTC 2 cut(s) 267, 320
SduI GDGCHC 1 cut(s) 168
SetI ASST 2 cut(s) 168, 376
SfoI GGCGCC 1 cut(s) 146
SinI GGWCC 2 cut(s) 28, 286
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 2 cut(s) 15, 414
SsiI CCGC 4 cut(s) 80, 91, 148, 368
SspDI GGCGCC 1 cut(s) 144
SspMI CTAG 2 cut(s) 360, 410
SstI GAGCTC 1 cut(s) 168
TaaI ACNGT 3 cut(s) 115, 238, 350
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 478
TaqII GACCGA 1 cut(s) 207
TasI AATT 2 cut(s) 15, 414
TatI WGTACW 2 cut(s) 291, 356
TauI GCSGC 1 cut(s) 150
TseFI GTSAC 4 cut(s) 115, 184, 200, 232
Tsp45I GTSAC 4 cut(s) 115, 184, 200, 232
VpaK11BI GGWCC 2 cut(s) 28, 286
XapI RAATTY 1 cut(s) 414
XspI CTAG 2 cut(s) 360, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.