pycom16g24980

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008374
Physical Location & Seq
Reverse (-)
26629508 .. 26634391
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGGAGGACTGGGAAGATGAGAAAGCCCCAACTCTCCTTTCTAAGGACCAACCAGTACATAAATGGGATGATGAAGATGTGGATGAAAATGATATTACAGAGTCATGGGAGGATTTAGATGAACCTGCTCCTTTGGAACCTGCAACAAGGCCTGTTTCTGAGAAGGCACCTAAGAAACCTGCAACAAAACCTGCTGAAAAAGCGACAGAAAAGAAAGGGAAAACCGTTCAAATAGAAAAGGAAGAGCCACTGGATCCTGTGGCTGGGAAACTTCGCCAACAAAGGCTGGTGGAAGAAGCGGATTATAAGTCCACTAAAGAATTGTTTTCCAGCGGAGGCAATTATAAAACCTTAGACAATTTCATTCCTAAATCTGAAAGTGACTTCCTGGAATATGCAGAACTTATTTCACATAAACTTCGTCCATTTGAGAAAAGTTTCCATTATATTGGTCTACTCAAGGCAGTAATGAGACTGTCAATGACTTCATTGAAAGGAGCAGATGCAAAAGAAGTTGCATCTTCCATTACAGCAATTGCAAATGAGAAAATAAAAGCAGAGAAGGAAGCAAATGCTGGTAAAAAGAAGACAGGCGCCAAGAAAAAGCAGCTGCTTGTTGATAAGCCGAATGATGATATAGCTGTTGATGACTATGATCCGCTTGATGACGATGATTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF3_subunit

Protein Analysis

228

Amino Acids

25.55

Weight (kDa)

4.83

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_subunit PF08597 2 - 227 5.8e-51 Translation initiation factor eIF3 subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 306, 345
Acc36I ACCTGC 4 cut(s) 133, 148, 187, 199
AccB1I GGYRCC 2 cut(s) 166, 593
AccI GTMKAC 1 cut(s) 454
AciI CCGC 3 cut(s) 299, 333, 659
AclWI GGATC 3 cut(s) 248, 261, 650
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 43, 263
AgsI TTSAA 2 cut(s) 230, 493
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 22, 54
AluBI AGCT 2 cut(s) 610, 641
AluI AGCT 2 cut(s) 610, 641
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 3 cut(s) 248, 261, 650
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 607, 610
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 46
AvaII GGWCC 1 cut(s) 46
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 166, 593
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 2 cut(s) 597, 619
BciT130I CCWGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 466
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 4 cut(s) 133, 148, 187, 199
BisI GCNGC 2 cut(s) 608, 611
BlsI GCNGC 2 cut(s) 609, 612
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 4 cut(s) 138, 168, 255, 595
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 493, 527
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 594
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 263
Bse1I ACTGG 3 cut(s) 14, 53, 255
BseBI CCWGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 73, 88
BseLI CCNNNNNNNGG 2 cut(s) 43, 263
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 3 cut(s) 14, 53, 255
BseXI GCAGC 2 cut(s) 597, 619
BseYI CCCAGC 1 cut(s) 263
BshFI GGCC 1 cut(s) 151
BshNI GGYRCC 2 cut(s) 166, 593
BslI CCNNNNNNNGG 2 cut(s) 43, 263
BsmAI GTCTC 1 cut(s) 466
BsnI GGCC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 253, 655
BspACI CCGC 3 cut(s) 299, 333, 659
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 138, 168, 255, 595
BspMI ACCTGC 4 cut(s) 133, 148, 187, 199
BspPI GGATC 3 cut(s) 248, 261, 650
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 2 cut(s) 166, 593
BsrI ACTGG 3 cut(s) 14, 53, 255
BssMI GATC 2 cut(s) 253, 655
BssNI GRCGYC 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 226, 477
Bst6I CTCTTC 1 cut(s) 237
BstACI GRCGYC 1 cut(s) 594
BstDEI CTNAG 4 cut(s) 42, 159, 171, 352
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 2 cut(s) 73, 88
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 2 cut(s) 256, 658
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 2 cut(s) 253, 655
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstV1I GCAGC 2 cut(s) 597, 619
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 151
BtsCI GGATG 2 cut(s) 73, 88
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 4 cut(s) 133, 148, 187, 199
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 56
CviAII CATG 1 cut(s) 105
CviJI RGCY 8 cut(s) 26, 151, 247, 263, 286, 610, 625, 641
CviKI_1 RGCY 8 cut(s) 26, 151, 247, 263, 286, 610, 625, 641
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 4 cut(s) 42, 159, 171, 352
DinI GGCGCC 1 cut(s) 595
DpnI GATC 2 cut(s) 255, 657
DpnII GATC 2 cut(s) 253, 655
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco147I AGGCCT 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
FaeI CATG 1 cut(s) 108
FatI CATG 1 cut(s) 104
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 608, 611
FokI GGATG 2 cut(s) 80, 95
Fsp4HI GCNGC 2 cut(s) 608, 611
GlaI GCGC 1 cut(s) 595
GluI GCNGC 2 cut(s) 608, 611
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 1 cut(s) 151
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 1 cut(s) 108
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HinfI GANTC 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 312, 455
Hpy188I TCNGA 2 cut(s) 160, 376
Hpy8I GTNNAC 2 cut(s) 312, 455
HpyAV CCTTC 2 cut(s) 157, 556
HpyCH4III ACNGT 2 cut(s) 226, 477
HpyCH4V TGCA 6 cut(s) 143, 182, 398, 506, 518, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 4 cut(s) 42, 159, 171, 352
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 1 cut(s) 108
HspAI GCGC 1 cut(s) 594
KasI GGCGCC 1 cut(s) 593
Kzo9I GATC 2 cut(s) 253, 655
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 133, 497
Lsp1109I GCAGC 2 cut(s) 597, 619
LweI GCATC 2 cut(s) 493, 527
MaeIII GTNAC 1 cut(s) 380
MalI GATC 2 cut(s) 255, 657
MboI GATC 2 cut(s) 253, 655
MboII GAAGA 6 cut(s) 26, 86, 254, 305, 513, 598
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 253
MluCI AATT 4 cut(s) 320, 340, 358, 534
Mly113I GGCGCC 1 cut(s) 594
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 2 cut(s) 103, 329
MroXI GAANNNNTTC 1 cut(s) 437
MspA1I CMGCKG 2 cut(s) 333, 610
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 200
NarI GGCGCC 1 cut(s) 594
NdeII GATC 2 cut(s) 253, 655
NlaIII CATG 1 cut(s) 108
NlaIV GGNNCC 4 cut(s) 138, 168, 255, 595
NmuCI GTSAC 1 cut(s) 380
PceI AGGCCT 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 2 cut(s) 609, 612
PleI GAGTC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 109
PsiI TTATAA 2 cut(s) 306, 345
Psp6I CCWGG 1 cut(s) 387
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 4 cut(s) 138, 168, 255, 595
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 610
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 237
SatI GCNGC 2 cut(s) 608, 611
Sau3AI GATC 2 cut(s) 253, 655
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 389
SetI ASST 8 cut(s) 127, 142, 172, 181, 193, 353, 612, 643
SfaNI GCATC 2 cut(s) 493, 527
SfoI GGCGCC 1 cut(s) 595
SinI GGWCC 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 4 cut(s) 320, 340, 358, 534
SseBI AGGCCT 1 cut(s) 151
SsiI CCGC 3 cut(s) 299, 333, 659
SspDI GGCGCC 1 cut(s) 593
StuI AGGCCT 1 cut(s) 151
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 226, 477
TasI AATT 4 cut(s) 320, 340, 358, 534
TatI WGTACW 1 cut(s) 55
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 2 cut(s) 607, 610
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 5 cut(s) 87, 99, 135, 352, 477
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.