pycom17g00550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005445
Physical Location & Seq
Reverse (-)
394736 .. 396042
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 849 bp
ATGAAGGAAAAGATGAGCTATGGCGTTTGGCGAAGGAGGCGGGTGTCCGTCGGATCAATTCTGATAGTCTGTGCGTTGTGTGCTGGAACGGGGCTGTTGATGCTCAACTTACGACAAGTCGATCCTCCGACGAGCCCCGATTTCTATATGCAGATTGGAAATTTGGATTCGGAGTCTGAAAACGGCGGCGGAGTTGAGCTGGAGACGGAGCCGCAGAGCAAGAGTAAGCGAAATTCGAGCTCGTGCGCGACGGTGGAGGAGATGGGAAAGGAGTTCGAAGGAGGAGGGTTTTGGGAGGAGAGTCTCAGAGTCAGGAAGTTTATTCAGCATCACTTTGACTTGAATGGTGCTTCCAGGGTAAGAAATCTTCCACCGGAACAGTTTTGTCGGCACGGATTTGTGATGGGTAAAGCATCAGAGGCGGGTTTCGGGAACGAAATGTACAAAATCTTAAGTGGTGCAGCGTTGAGTATAATGTTGAACCGGTCCCCGATCATTGGTCAAACCAGGGGAAAATTCCCTTTTGAGGATTACATTTCCTATTCCAATTTTTCCTTCACCATCAGAGAAATTAAGCATCTTTGGAGACTTAATAAATGTGCAAACAAGTATGGAAGGCAACTGGTTATGAGAAGTGATGACTTTGAGAAGCCGGCACAGACCAATGTTCTTTGTAGCAACTGGATAGAATGGAAGCAACCCATTATATGGTTCCAAGGGACGAACGATGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATCCACCCTGAGATGAGGAATGCTGCTTCTACTTTATTTGGGAAGCCAGAGGATCGATCCATTCTCGGCCTAATGTATTTGGGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.97

Weight (kDa)

8.85

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 78 - 271 2e-103 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 708
AccII CGCG 1 cut(s) 248
AciI CCGC 5 cut(s) 40, 186, 189, 212, 422
AclWI GGATC 4 cut(s) 61, 116, 810, 819
AcsI RAATTY 3 cut(s) 160, 232, 515
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 3 cut(s) 497, 526, 708
AflII CTTAAG 1 cut(s) 451
AgeI ACCGGT 1 cut(s) 483
AgsI TTSAA 3 cut(s) 343, 481, 754
AjnI CCWGG 2 cut(s) 353, 506
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AluBI AGCT 4 cut(s) 18, 199, 240, 846
AluI AGCT 4 cut(s) 18, 199, 240, 846
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 3 cut(s) 197, 308, 580
AlwI GGATC 4 cut(s) 61, 116, 810, 819
AoxI GGCC 1 cut(s) 826
ApeKI GCWGC 3 cut(s) 461, 737, 782
ApoI RAATTY 3 cut(s) 160, 232, 515
AsiGI ACCGGT 1 cut(s) 483
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 486
AsuHPI GGTGA 1 cut(s) 550
AsuII TTCGAA 1 cut(s) 276
AvaII GGWCC 1 cut(s) 486
BanII GRGCYC 2 cut(s) 137, 242
BarI GAAGNNNNNNTAC 2 cut(s) 351, 383
BauI CACGAG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 3 cut(s) 473, 749, 769
BccI CCATC 3 cut(s) 256, 397, 569
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 2 cut(s) 355, 508
BcoDI GTCTC 3 cut(s) 197, 308, 580
BfrI CTTAAG 1 cut(s) 451
BisI GCNGC 5 cut(s) 187, 212, 462, 738, 783
BlsI GCNGC 5 cut(s) 188, 213, 463, 739, 784
Bme1390I CCNGG 2 cut(s) 355, 508
Bme18I GGWCC 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 486
BmiI GGNNCC 3 cut(s) 210, 488, 713
BmrFI CCNGG 2 cut(s) 355, 508
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 5 cut(s) 90, 337, 422, 586, 718
BmuI ACTGGG 1 cut(s) 736
BpmI CTGGAG 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 814
BsaJI CCNNGG 3 cut(s) 354, 507, 715
BsaWI WCCGGW 2 cut(s) 373, 483
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 526, 708
Bse118I RCCGGY 2 cut(s) 483, 652
Bse1I ACTGG 3 cut(s) 627, 686, 742
BseBI CCWGG 2 cut(s) 355, 508
BseCI ATCGAT 1 cut(s) 814
BseDI CCNNGG 3 cut(s) 354, 507, 715
BseLI CCNNNNNNNGG 3 cut(s) 497, 526, 708
BseMII CTCAG 2 cut(s) 319, 759
BseNI ACTGG 3 cut(s) 627, 686, 742
BseRI GAGGAG 3 cut(s) 272, 297, 311
BseXI GCAGC 3 cut(s) 473, 749, 769
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 1 cut(s) 828
BshTI ACCGGT 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 3 cut(s) 374, 484, 653
BslFI GGGAC 2 cut(s) 472, 733
BslI CCNNNNNNNGG 3 cut(s) 497, 526, 708
BsmAI GTCTC 3 cut(s) 197, 308, 580
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 472, 733
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 1 cut(s) 828
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 137, 242
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 5 cut(s) 53, 121, 492, 811, 815
BspACI CCGC 5 cut(s) 40, 186, 189, 212, 422
BspANI GGCC 1 cut(s) 828
BspCNI CTCAG 2 cut(s) 318, 760
BspDI ATCGAT 1 cut(s) 814
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 210, 488, 713
BspPI GGATC 4 cut(s) 61, 116, 810, 819
BspT104I TTCGAA 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 451
BsrFI RCCGGY 2 cut(s) 483, 652
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 3 cut(s) 627, 686, 742
BssAI RCCGGY 2 cut(s) 483, 652
BssECI CCNNGG 3 cut(s) 354, 507, 715
BssMI GATC 5 cut(s) 53, 121, 492, 811, 815
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 715
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 2 cut(s) 355, 508
Bst4CI ACNGT 2 cut(s) 253, 381
BstAFI CTTAAG 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 441
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 2 cut(s) 305, 768
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 5 cut(s) 56, 124, 495, 814, 818
BstMAI GTCTC 3 cut(s) 197, 308, 580
BstMBI GATC 5 cut(s) 53, 121, 492, 811, 815
BstMWI GCNNNNNNNGC 5 cut(s) 37, 80, 100, 419, 737
BstNI CCWGG 2 cut(s) 355, 508
BstSCI CCNGG 2 cut(s) 353, 506
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 473, 749, 769
Bsu15I ATCGAT 1 cut(s) 814
BsuRI GGCC 1 cut(s) 828
BsuTUI ATCGAT 1 cut(s) 814
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 2 cut(s) 483, 652
Cfr13I GGNCC 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 814
Csp6I GTAC 1 cut(s) 442
CspAI ACCGGT 1 cut(s) 483
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 305, 768
DpnI GATC 5 cut(s) 55, 123, 494, 813, 817
DpnII GATC 5 cut(s) 53, 121, 492, 811, 815
EciI GGCGGA 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 715
Eco24I GRGCYC 2 cut(s) 137, 242
Eco47I GGWCC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 353, 506
EcoT14I CCWWGG 1 cut(s) 715
EcoT38I GRGCYC 2 cut(s) 137, 242
ErhI CCWWGG 1 cut(s) 715
Esp3I CGTCTC 1 cut(s) 197
FaiI YATR 8 cut(s) 21, 147, 149, 473, 612, 629, 707, 709
FaqI GGGAC 2 cut(s) 472, 733
FauI CCCGC 2 cut(s) 33, 415
Fnu4HI GCNGC 5 cut(s) 187, 212, 462, 738, 783
FriOI GRGCYC 2 cut(s) 137, 242
Fsp4HI GCNGC 5 cut(s) 187, 212, 462, 738, 783
GlaI GCGC 1 cut(s) 247
GluI GCNGC 5 cut(s) 187, 212, 462, 738, 783
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 828
HapII CCGG 3 cut(s) 374, 484, 653
HhaI GCGC 1 cut(s) 248
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 4 cut(s) 167, 173, 301, 309
HpaII CCGG 3 cut(s) 374, 484, 653
HphI GGTGA 1 cut(s) 550
Hpy188I TCNGA 8 cut(s) 53, 63, 129, 172, 178, 308, 418, 566
Hpy188III TCNNGA 3 cut(s) 313, 430, 751
Hpy99I CGWCG 3 cut(s) 53, 133, 253
HpyAV CCTTC 4 cut(s) 27, 272, 565, 609
HpyCH4III ACNGT 2 cut(s) 253, 381
HpyCH4V TGCA 3 cut(s) 151, 461, 602
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 80, 100, 419, 737
HpyF3I CTNAG 2 cut(s) 305, 768
HspAI GCGC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 652
KroNI GCCGGC 1 cut(s) 654
Kzo9I GATC 5 cut(s) 53, 121, 492, 811, 815
LmnI GCTCC 2 cut(s) 208, 843
Lsp1109I GCAGC 3 cut(s) 473, 749, 769
LweI GCATC 5 cut(s) 90, 337, 422, 586, 718
MalI GATC 5 cut(s) 55, 123, 494, 813, 817
MboI GATC 5 cut(s) 53, 121, 492, 811, 815
MboII GAAGA 2 cut(s) 359, 766
MhlI GDGCHC 2 cut(s) 137, 242
MluCI AATT 6 cut(s) 57, 160, 232, 515, 547, 570
MlyI GAGTC 3 cut(s) 182, 310, 318
MmeI TCCRAC 2 cut(s) 31, 152
MroNI GCCGGC 1 cut(s) 652
MseI TTAA 3 cut(s) 452, 573, 591
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 3 cut(s) 374, 484, 653
MspR9I CCNGG 2 cut(s) 355, 508
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 2 cut(s) 355, 508
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 5 cut(s) 37, 80, 100, 419, 737
NaeI GCCGGC 1 cut(s) 654
NdeII GATC 5 cut(s) 53, 121, 492, 811, 815
NgoMIV GCCGGC 1 cut(s) 652
NlaIV GGNNCC 3 cut(s) 210, 488, 713
NmeAIII GCCGAG 1 cut(s) 804
NspV TTCGAA 1 cut(s) 276
PctI GAATGC 1 cut(s) 784
PdiI GCCGGC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 708
PinAI ACCGGT 1 cut(s) 483
PkrI GCNGC 5 cut(s) 188, 213, 463, 739, 784
PleI GAGTC 3 cut(s) 181, 309, 317
PpsI GAGTC 3 cut(s) 181, 309, 317
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 353, 506
PspGI CCWGG 2 cut(s) 353, 506
PspN4I GGNNCC 3 cut(s) 210, 488, 713
PspPI GGNCC 1 cut(s) 486
PsrI GAACNNNNNNTAC 2 cut(s) 425, 457
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SacI GAGCTC 1 cut(s) 242
SaqAI TTAA 3 cut(s) 452, 573, 591
SatI GCNGC 5 cut(s) 187, 212, 462, 738, 783
Sau3AI GATC 5 cut(s) 53, 121, 492, 811, 815
Sau96I GGNCC 1 cut(s) 486
SchI GAGTC 3 cut(s) 182, 310, 318
ScrFI CCNGG 2 cut(s) 355, 508
SduI GDGCHC 2 cut(s) 137, 242
SetI ASST 4 cut(s) 20, 201, 242, 848
SfaNI GCATC 5 cut(s) 90, 337, 422, 586, 718
SfuI TTCGAA 1 cut(s) 276
SinI GGWCC 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 6 cut(s) 57, 160, 232, 515, 547, 570
SsiI CCGC 5 cut(s) 40, 186, 189, 212, 422
SstI GAGCTC 1 cut(s) 242
StyD4I CCNGG 2 cut(s) 353, 506
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 2 cut(s) 253, 381
TaqI TCGA 4 cut(s) 120, 236, 276, 814
TasI AATT 6 cut(s) 57, 160, 232, 515, 547, 570
TatI WGTACW 1 cut(s) 441
TauI GCSGC 2 cut(s) 189, 214
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 3 cut(s) 452, 573, 591
Tru9I TTAA 3 cut(s) 452, 573, 591
TseI GCWGC 3 cut(s) 461, 737, 782
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 37, 221, 408
Van91I CCANNNNNTGG 1 cut(s) 708
Vha464I CTTAAG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 3 cut(s) 160, 232, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.