Rw2G054910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005445
Physical Location & Seq
Forward (+)
82483938 .. 82486729
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054910.1

Sequence Viewer

Length: 1674 bp
ATGATTAGGAGGCATGGCGTATGGAGAAGGAGACGAGTTTCTGTAGGGTCAATTCTGATATTCGGTGCAATTTTACCTGTCCATGATGATTCTCCAACGACAACGAGCCGCGATTTCCCTGTTGAAATTCACGACTCGGTTTCGATTTCGGAGTCGGAGTCGGAGTCTGAGAGCGGTGCTGGTGCTGGAGTTGGGGTTGAGTCGGAGACTCGGAGCAGCGAGAGTAAGGGTAAGAGAAAATCGAGCTCGTGTGCTTCGGTGGAAGGGATGGGAAAGGAGTTTGAAGGAGGGTTTTGGGATGAAAGTTTCAGAGTGAGGAAACTTATTCAAAATCACTTTGAATTGAATGGTGCTTCGAGGGTGCGGAATCTTCCTCCACAACAGTTTTGCCAGCATGGTTTTGTGCTTGGCAAATCAGCAGAGGCAGGCTTCGGGAATGAAATGTACAAGATCTTAAGTGGTGCAGCACTGAGTGTGATGTTAAACCGGTCCCTGATTATTGGGCAAACCAGGGGAAAATTCCCTTTTGGGGATTACATTTCTTATTCCAACCTTTCTTTTACAATGAAAGAAATCAAGCATCTGTGGAGACTTAATAAATGTGTAAAGAAGTATGGAAGGCAACTGATCATGAGGACTGATGACTTTGAGAAACCAATGAAGACAAATGTCATGTGTAGCAACTGGAGGAGATGGAAGCAACCTATTATATGGTTCCAAGGTACAAATGACGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATACATCCAGAGATGAGGAAGGCTGCTTCTAATTTATTTGGAAAGCCAGAGGCTCTCCATTCTCGGCCTAATGTATTTGGAGAGCTGATGAGAGTGCTTATATCTCCTTCTGAAGATGTTGAGCAAGCAATCAATTGGGTTATCGGTGGAGTGGATCCTGATATTTCTCTGCACATGCGAATGCTCATGAATAGGTCGGTGAGAGCTGCACAGGCAGCACTAAACTGCATCAGAAAAGCTGTGCGCAATCTTGGTAAAGCCTCAAGGCCCAGGGTGGTCTTAGTGTCAGATACCCCCTCTCTTATAAAAAGTATTACACCGAATATAAGCAAGTTTGCTGAGGTAATTCATTTTGATTACGAACTTTTCAAAGGAAATATATCTGACAGCGGTAAAAGATTGCGAAGTTTAGAATTTAGAGCAAAAGATTGGGGCCCAGCACCCAGATGGGTCGCCTTTGTGGACTTCTTTCTTGCATCACGGGCAAAACATGCTGTTGTGTCTGGTGCTCACAGGCGTGTTGGGACTACATATGCTCAGTTAATTGCAGCACTGGCTGCAGCAAACAATCTAGGGGACAGTCCAACTGGTTCGAATTTTTCGTTCTTAAGCAGCTTTCAAAGTGATTTGTTGAGAGAAGGTTTAAGATTTCAGATTGGTTGGGGACATGTGTGGAACAGATTTGCAGGTCCCCTTAGTTGTCACAACCAGCATAACCAATGTGCTTTCACACCACTTCTCCCTCCTGCATGGTGGGATGGTCTTTGGCAATCTCCACTAACAAGGGATATTAATAGGTTGGCAGACTACGGCATCCAGTTAACTGGTTTTGGCACCATTGATGAAACACATCTTCAGTCTTTTTGTAGCTCAAAGAAAGTTGCTGTGAAAACTATTCCGTACATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

557

Amino Acids

62.44

Weight (kDa)

9.76

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 80 - 553 1.7e-264 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1067
Acc16I TGCGCA 1 cut(s) 1007
Acc36I ACCTGC 1 cut(s) 1439
AccB1I GGYRCC 1 cut(s) 1595
AccBSI CCGCTC 1 cut(s) 174
AccII CGCG 1 cut(s) 111
AciI CCGC 4 cut(s) 109, 174, 364, 1152
AclWI GGATC 2 cut(s) 911, 924
AcsI RAATTY 4 cut(s) 126, 518, 1175, 1357
AcuI CTGAAG 2 cut(s) 894, 1601
AdeI CACNNNGTG 1 cut(s) 473
AfaI GTAC 3 cut(s) 446, 724, 1664
AfiI CCNNNNNNNGG 1 cut(s) 529
AflII CTTAAG 2 cut(s) 454, 1369
AflIII ACRYGT 1 cut(s) 1429
AgeI ACCGGT 1 cut(s) 486
AgsI TTSAA 8 cut(s) 125, 284, 329, 341, 346, 757, 1132, 1382
AjnI CCWGG 2 cut(s) 509, 1031
AleI CACNNNNGTG 1 cut(s) 1278
AluBI AGCT 6 cut(s) 246, 847, 968, 1001, 1377, 1632
AluI AGCT 6 cut(s) 246, 847, 968, 1001, 1377, 1632
Alw21I GWGCWC 2 cut(s) 248, 1273
Alw26I GTCTC 3 cut(s) 25, 200, 583
AlwI GGATC 2 cut(s) 911, 924
AoxI GGCC 3 cut(s) 827, 1028, 1195
ApaI GGGCCC 1 cut(s) 1199
ApoI RAATTY 4 cut(s) 126, 518, 1175, 1357
AseI ATTAAT 1 cut(s) 1554
AsiGI ACCGGT 1 cut(s) 486
AspLEI GCGC 1 cut(s) 1008
AspS9I GGNCC 5 cut(s) 489, 1029, 1195, 1196, 1451
AsuHPI GGTGA 1 cut(s) 973
AsuII TTCGAA 1 cut(s) 1355
AvaII GGWCC 2 cut(s) 489, 1451
BaeGI GKGCMC 1 cut(s) 1199
BamHI GGATCC 1 cut(s) 916
BanI GGYRCC 1 cut(s) 1595
BanII GRGCYC 2 cut(s) 248, 1199
BauI CACGAG 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 2 cut(s) 248, 1273
BbvCI CCTCAGC 1 cut(s) 1101
BccI CCATC 4 cut(s) 262, 687, 1203, 1514
BceAI ACGGC 1 cut(s) 1588
BciT130I CCWGG 2 cut(s) 511, 1033
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 3 cut(s) 25, 200, 583
BfaI CTAG 1 cut(s) 1334
BfmI CTRYAG 2 cut(s) 42, 1320
BfrI CTTAAG 2 cut(s) 454, 1369
BfuAI ACCTGC 1 cut(s) 1439
BglII AGATCT 1 cut(s) 450
Bme1390I CCNGG 2 cut(s) 511, 1033
Bme18I GGWCC 2 cut(s) 489, 1451
BmgT120I GGNCC 5 cut(s) 489, 1029, 1195, 1196, 1451
BmiI GGNNCC 7 cut(s) 491, 716, 918, 1196, 1197, 1453, 1597
BmrFI CCNGG 2 cut(s) 511, 1033
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 4 cut(s) 589, 999, 1247, 1584
BmuI ACTGGG 1 cut(s) 739
BoxI GACNNNNGTC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 2 cut(s) 207, 706
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1355
BpuEI CTTGAG 1 cut(s) 1009
BsaJI CCNNGG 4 cut(s) 510, 718, 1031, 1032
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 8 cut(s) 143, 149, 173, 179, 180, 210, 1485, 1515
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 6 cut(s) 689, 745, 1320, 1354, 1579, 1591
BseBI CCWGG 2 cut(s) 511, 1033
BseDI CCNNGG 4 cut(s) 510, 718, 1031, 1032
BseGI GGATG 5 cut(s) 273, 304, 766, 1525, 1575
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 4 cut(s) 159, 461, 1092, 1313
BseNI ACTGG 6 cut(s) 689, 745, 1320, 1354, 1579, 1591
BseRI GAGGAG 1 cut(s) 703
BseSI GKGCMC 1 cut(s) 1199
BseYI CCCAGC 1 cut(s) 1198
BsgI GTGCAG 3 cut(s) 483, 917, 954
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 829, 1030, 1197
BshNI GGYRCC 1 cut(s) 1595
BshTI ACCGGT 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 248, 1273
BsiSI CCGG 1 cut(s) 487
BslFI GGGAC 5 cut(s) 475, 1300, 1352, 1437, 1440
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 3 cut(s) 25, 200, 583
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 5 cut(s) 475, 1300, 1352, 1437, 1440
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 3 cut(s) 829, 1030, 1197
Bsp119I TTCGAA 1 cut(s) 1355
Bsp120I GGGCCC 1 cut(s) 1195
Bsp1286I GDGCHC 3 cut(s) 248, 1199, 1273
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 3 cut(s) 450, 627, 916
BspACI CCGC 4 cut(s) 109, 174, 364, 1152
BspANI GGCC 3 cut(s) 829, 1030, 1197
BspCNI CTCAG 4 cut(s) 160, 462, 1093, 1312
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 2 cut(s) 630, 948
BspLI GGNNCC 7 cut(s) 491, 716, 918, 1196, 1197, 1453, 1597
BspMAI CTGCAG 1 cut(s) 1324
BspMI ACCTGC 1 cut(s) 1439
BspPI GGATC 2 cut(s) 911, 924
BspT104I TTCGAA 1 cut(s) 1355
BspT107I GGYRCC 1 cut(s) 1595
BspTI CTTAAG 2 cut(s) 454, 1369
BsrBI CCGCTC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 486
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 6 cut(s) 689, 745, 1320, 1354, 1579, 1591
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 4 cut(s) 510, 718, 1031, 1032
BssMI GATC 3 cut(s) 450, 627, 916
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 2 cut(s) 511, 1033
Bst4CI ACNGT 2 cut(s) 384, 1343
BstAFI CTTAAG 2 cut(s) 454, 1369
BstAPI GCANNNNNTGC 2 cut(s) 1253, 1319
BstAUI TGTACA 1 cut(s) 444
BstBI TTCGAA 1 cut(s) 1355
BstC8I GCNNGC 3 cut(s) 392, 427, 888
BstDEI CTNAG 6 cut(s) 168, 470, 1042, 1101, 1299, 1457
BstF5I GGATG 5 cut(s) 273, 304, 766, 1525, 1575
BstFNI CGCG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 1008
BstKTI GATC 3 cut(s) 453, 630, 919
BstMAI GTCTC 3 cut(s) 25, 200, 583
BstMBI GATC 3 cut(s) 450, 627, 916
BstMWI GCNNNNNNNGC 7 cut(s) 740, 974, 977, 1244, 1253, 1316, 1319
BstNI CCWGG 2 cut(s) 511, 1033
BstNSI RCATGY 3 cut(s) 940, 1256, 1433
BstPAI GACNNNNGTC 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 509, 1031
BstSFI CTRYAG 2 cut(s) 42, 1320
BstSLI GKGCMC 1 cut(s) 1199
BstUI CGCG 1 cut(s) 111
BstV2I GAAGAC 1 cut(s) 668
BstX2I RGATCY 2 cut(s) 450, 916
BstXI CCANNNNNNTGG 1 cut(s) 1586
BstYI RGATCY 2 cut(s) 450, 916
BsuRI GGCC 3 cut(s) 829, 1030, 1197
BtsCI GGATG 5 cut(s) 273, 304, 766, 1525, 1575
BtsIMutI CAGTG 2 cut(s) 467, 1313
BveI ACCTGC 1 cut(s) 1439
Cac8I GCNNGC 3 cut(s) 392, 427, 888
CciI TCATGA 2 cut(s) 630, 948
CfoI GCGC 1 cut(s) 1008
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 5 cut(s) 489, 1029, 1195, 1196, 1451
CseI GACGC 1 cut(s) 740
Csp6I GTAC 3 cut(s) 445, 723, 1663
CspAI ACCGGT 1 cut(s) 486
CviQI GTAC 3 cut(s) 445, 723, 1663
DdeI CTNAG 6 cut(s) 168, 470, 1042, 1101, 1299, 1457
DpnI GATC 3 cut(s) 452, 629, 918
DpnII GATC 3 cut(s) 450, 627, 916
DraIII CACNNNGTG 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 718
Eco24I GRGCYC 2 cut(s) 248, 1199
Eco47I GGWCC 2 cut(s) 489, 1451
Eco53kI GAGCTC 1 cut(s) 246
Eco57I CTGAAG 2 cut(s) 894, 1601
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 2 cut(s) 1195, 1451
EcoRII CCWGG 2 cut(s) 509, 1031
EcoT14I CCWWGG 1 cut(s) 718
EcoT38I GRGCYC 2 cut(s) 248, 1199
ErhI CCWWGG 1 cut(s) 718
Esp3I CGTCTC 1 cut(s) 25
FaqI GGGAC 5 cut(s) 475, 1300, 1352, 1437, 1440
FauNDI CATATG 1 cut(s) 1294
FbaI TGATCA 1 cut(s) 627
FokI GGATG 5 cut(s) 280, 311, 753, 1532, 1562
FriOI GRGCYC 2 cut(s) 248, 1199
FspBI CTAG 1 cut(s) 1334
FspI TGCGCA 1 cut(s) 1007
GlaI GCGC 1 cut(s) 1007
GsaI CCCAGC 1 cut(s) 1202
GsuI CTGGAG 2 cut(s) 207, 706
HaeIII GGCC 3 cut(s) 829, 1030, 1197
HapII CCGG 1 cut(s) 487
HgaI GACGC 1 cut(s) 740
HhaI GCGC 1 cut(s) 1008
Hin6I GCGC 1 cut(s) 1006
HinP1I GCGC 1 cut(s) 1006
HincII GTYRAC 1 cut(s) 1584
HindII GTYRAC 1 cut(s) 1584
HinfI GANTC 8 cut(s) 89, 134, 152, 158, 164, 200, 208, 367
HpaI GTTAAC 1 cut(s) 1584
HpaII CCGG 1 cut(s) 487
HphI GGTGA 1 cut(s) 973
Hpy166II GTNNAC 2 cut(s) 1225, 1584
Hpy188III TCNNGA 7 cut(s) 131, 433, 631, 754, 770, 920, 949
Hpy8I GTNNAC 2 cut(s) 1225, 1584
HpyAV CCTTC 7 cut(s) 21, 257, 278, 612, 775, 879, 1394
HpyCH4III ACNGT 2 cut(s) 384, 1343
HpyF10VI GCNNNNNNNGC 7 cut(s) 740, 974, 977, 1244, 1253, 1316, 1319
HpyF3I CTNAG 6 cut(s) 168, 470, 1042, 1101, 1299, 1457
HspAI GCGC 1 cut(s) 1006
Ksp22I TGATCA 1 cut(s) 627
KspAI GTTAAC 1 cut(s) 1584
Kzo9I GATC 3 cut(s) 450, 627, 916
LmnI GCTCC 1 cut(s) 213
LweI GCATC 4 cut(s) 589, 999, 1247, 1584
MaeI CTAG 1 cut(s) 1334
MaeIII GTNAC 1 cut(s) 1463
MalI GATC 3 cut(s) 452, 629, 918
MbiI CCGCTC 1 cut(s) 174
MboI GATC 3 cut(s) 450, 627, 916
MboII GAAGA 5 cut(s) 362, 673, 769, 887, 1607
MfeI CAATTG 1 cut(s) 895
MflI RGATCY 2 cut(s) 450, 916
MhlI GDGCHC 3 cut(s) 248, 1199, 1273
MlyI GAGTC 6 cut(s) 128, 161, 167, 173, 202, 209
MmeI TCCRAC 6 cut(s) 119, 135, 141, 183, 573, 1370
MseI TTAA 8 cut(s) 455, 482, 594, 1304, 1370, 1406, 1554, 1583
MslI CAYNNNNRTG 3 cut(s) 941, 1207, 1278
MspA1I CMGCKG 1 cut(s) 1152
MspCI CTTAAG 2 cut(s) 454, 1369
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 2 cut(s) 511, 1033
MunI CAATTG 1 cut(s) 895
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 2 cut(s) 511, 1033
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 7 cut(s) 740, 974, 977, 1244, 1253, 1316, 1319
NdeI CATATG 1 cut(s) 1294
NdeII GATC 3 cut(s) 450, 627, 916
NlaIV GGNNCC 7 cut(s) 491, 716, 918, 1196, 1197, 1453, 1597
NmeAIII GCCGAG 1 cut(s) 805
NmuCI GTSAC 1 cut(s) 1463
NsbI TGCGCA 1 cut(s) 1007
NspI RCATGY 3 cut(s) 940, 1256, 1433
NspV TTCGAA 1 cut(s) 1355
OliI CACNNNNGTG 1 cut(s) 1278
PagI TCATGA 2 cut(s) 630, 948
PasI CCCWGGG 1 cut(s) 1032
PciI ACATGT 1 cut(s) 1429
PcsI WCGNNNNNNNCGW 1 cut(s) 1361
PctI GAATGC 1 cut(s) 948
PfeI GAWTC 2 cut(s) 89, 367
PinAI ACCGGT 1 cut(s) 486
PleI GAGTC 6 cut(s) 128, 160, 166, 172, 202, 208
PpsI GAGTC 6 cut(s) 128, 160, 166, 172, 202, 208
PpuMI RGGWCCY 1 cut(s) 1451
PscI ACATGT 1 cut(s) 1429
PshAI GACNNNNGTC 1 cut(s) 668
PshBI ATTAAT 1 cut(s) 1554
PsiI TTATAA 1 cut(s) 1067
Psp124BI GAGCTC 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 1451
Psp6I CCWGG 2 cut(s) 509, 1031
PspFI CCCAGC 1 cut(s) 1198
PspGI CCWGG 2 cut(s) 509, 1031
PspN4I GGNNCC 7 cut(s) 491, 716, 918, 1196, 1197, 1453, 1597
PspOMI GGGCCC 1 cut(s) 1195
PspPI GGNCC 5 cut(s) 489, 1029, 1195, 1196, 1451
PspPPI RGGWCCY 1 cut(s) 1451
PstI CTGCAG 1 cut(s) 1324
PsuI RGATCY 2 cut(s) 450, 916
RsaI GTAC 3 cut(s) 446, 724, 1664
RsaNI GTAC 3 cut(s) 445, 723, 1663
RseI CAYNNNNRTG 3 cut(s) 941, 1207, 1278
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 8 cut(s) 455, 482, 594, 1304, 1370, 1406, 1554, 1583
Sau3AI GATC 3 cut(s) 450, 627, 916
Sau96I GGNCC 5 cut(s) 489, 1029, 1195, 1196, 1451
SchI GAGTC 6 cut(s) 128, 161, 167, 173, 202, 209
ScrFI CCNGG 2 cut(s) 511, 1033
SduI GDGCHC 3 cut(s) 248, 1199, 1273
SfaNI GCATC 4 cut(s) 589, 999, 1247, 1584
SfcI CTRYAG 2 cut(s) 42, 1320
SfuI TTCGAA 1 cut(s) 1355
SinI GGWCC 2 cut(s) 489, 1451
SmiMI CAYNNNNRTG 3 cut(s) 941, 1207, 1278
SmlI CTYRAG 3 cut(s) 454, 1024, 1369
SmoI CTYRAG 3 cut(s) 454, 1024, 1369
SsiI CCGC 4 cut(s) 109, 174, 364, 1152
SspMI CTAG 1 cut(s) 1334
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 509, 1031
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 2 cut(s) 384, 1343
TaqI TCGA 4 cut(s) 143, 242, 356, 1355
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 2 cut(s) 89, 367
Tru1I TTAA 8 cut(s) 455, 482, 594, 1304, 1370, 1406, 1554, 1583
Tru9I TTAA 8 cut(s) 455, 482, 594, 1304, 1370, 1406, 1554, 1583
TscAI CASTG 2 cut(s) 474, 1320
TseFI GTSAC 1 cut(s) 1463
Tsp45I GTSAC 1 cut(s) 1463
TspDTI ATGAA 7 cut(s) 315, 453, 581, 674, 965, 1100, 1620
TspGWI ACGGA 1 cut(s) 1650
TspRI CASTG 2 cut(s) 474, 1320
Vha464I CTTAAG 2 cut(s) 454, 1369
VpaK11BI GGWCC 2 cut(s) 489, 1451
VspI ATTAAT 1 cut(s) 1554
XapI RAATTY 4 cut(s) 126, 518, 1175, 1357
XceI RCATGY 3 cut(s) 940, 1256, 1433
XcmI CCANNNNNNNNNTGG 1 cut(s) 1206
XspI CTAG 1 cut(s) 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.