RchiOBHm_Chr2g0176111

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005445
Physical Location & Seq
Forward (+)
88040769 .. 88043892
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54314

Sequence Viewer

Length: 1734 bp
ATGATGAGGAGGCATGGCGTATGGAGAAGGAGACGAGTTTCTGTAGGGTCAATTCTGATATTCGGTGCAATTTTTGCCGGAATCGGATTGCTGATTCTCAACTTAAGACCTGTCCATGATGATTCTCCAACGACAACGAGCCGCGATTTCCCTGTTGAAATTCACGACTCGGTTTCGATTTCGGAGTCGGAGTCGGACTCTGAGAGCGGTGCTGGTGCTGATGCTGGAGTTGGGGTTGAGTCGGAGACTCGGAGCAGCGAGAGCGAGAGTAAGAGTAAGAGAAAATCGAGCTCGTGTGCTTCGGTGCAAGAGATGGGAAAGGAGTTTGAAGGAGGGTTTTGGGATGAAAGTTTCAGAGTGAGGAAACTTATTCAAAATCACTTTGAATTGAATGGTGCTTCGAGGGTGCGGAATCTTCCTCCACAACAGTTTTGCCAGCATGGTTTTGTGCTTGGCAAATCAGCAGAGGCAGGCTTCGGGAATGAAATGTACAAGATCTTAAGTGGTGCAGCACTGAGTGTGATGTTAAACCGGTCCCTGATTATTGGGCAAACCAGGCATATTATTGCTGAGGGAAAATTCCCTTTTGGGGATTACATTTCTTATTCCAACCTTTCTTTTACAATGAAAGAAATCAAGCATCTGTGGAGACTTAATAAATGTGTAAAGAAGTATGGAAGGCAACTGATTATGAGGACTGATGACTTTGAGAAACCAATGAAGACAAATGTCCTGTGTAGCAACTGGAGGAGATGGAAGCAACCTATTATATGGTTCCAAGGTACAAATGACGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATACATCCAGAGATGAGGAAGGCTGCTTCTAATTTATTTGGAAAGCCAGAGGCTCTCCATTCTCGGCCTAATGTATTTGGAGAGCTGATGAGAGTGCTTATATCTCCTTCTGAAGATGTTGAGCAAGCAATCAATTGGGTTATCGGTGGAGTGGATCCTGATATTTCTCTGCACATGCGAATGCTCATGAATAGGTCGGTGAGAGCTGCACAGGCAGCACTAAACTGCATCAGAAAAGCTGTGCGCAATCTTGGTAAAGCCTCAAGGCCCAGGGTGGTCTTAGTGTCAGATACCCCCTCTCTTATAAAAAGTATTACACCGAATATAAGCAAGTTTGCTGAGGTAATTCATTTTGATTACGAACTTTTCAAAGGAAATATATCTGACGGCGGTAAAAGATTGCGAAGTTTAGAATTTAGAGCAAAAGATTGGGGCCCAGCACCCAGATGGGTCGCCTTTGTGGACTTCTTTCTTGCATCACGGGCAAAACATGCTGTTGTGTCTGGTGCTCACAGGCGTGTTGGGACTACATATGCTCAGTTAATTGCAGCACTGGCTGCAGCAAACAATCTAGGGGACAGTCCAACTGGTTCGAATTTTTCGTTCTTAAGCAGCTTTCAAAGTGATTTGTTGAGAGAAGGTTTAAGATTTCAGATTGGTTGGGGACATGTGTGGAACCGATTTGCAGGTCCCCTTAGTTGTCACAACCAGCATAACCAATGTGCTTTCACACCACTTCTCCCTCCTGCATGGTGGGATGGTCTTTGGCAATCTCCACTAACAAGGGATATTAATAGGTTGGCAGACTACGGCATCCAGTTAACTGGTTTTGGCACCATTGATGAAACACATCTTCAGTCTTTTTGTAGCTCAAAGAAAGTTGCTGTGAAAACTATTCCGTACATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

577

Amino Acids

64.63

Weight (kDa)

9.67

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 95 - 573 2.8e-266 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1127
Acc16I TGCGCA 1 cut(s) 1067
Acc36I ACCTGC 1 cut(s) 1499
AccB1I GGYRCC 1 cut(s) 1655
AccBSI CCGCTC 1 cut(s) 207
AccII CGCG 1 cut(s) 144
AciI CCGC 4 cut(s) 142, 207, 409, 1212
AclWI GGATC 2 cut(s) 971, 984
AcsI RAATTY 4 cut(s) 159, 578, 1235, 1417
AcuI CTGAAG 2 cut(s) 954, 1661
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 3 cut(s) 491, 784, 1724
AfiI CCNNNNNNNGG 1 cut(s) 589
AflII CTTAAG 3 cut(s) 103, 499, 1429
AflIII ACRYGT 1 cut(s) 1489
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 8 cut(s) 158, 329, 374, 386, 391, 817, 1192, 1442
AjnI CCWGG 2 cut(s) 554, 1091
AleI CACNNNNGTG 1 cut(s) 1338
AluBI AGCT 6 cut(s) 291, 907, 1028, 1061, 1437, 1692
AluI AGCT 6 cut(s) 291, 907, 1028, 1061, 1437, 1692
Alw21I GWGCWC 2 cut(s) 293, 1333
Alw26I GTCTC 3 cut(s) 25, 239, 643
AlwI GGATC 2 cut(s) 971, 984
AoxI GGCC 3 cut(s) 887, 1088, 1255
ApaI GGGCCC 1 cut(s) 1259
ApoI RAATTY 4 cut(s) 159, 578, 1235, 1417
AseI ATTAAT 1 cut(s) 1614
AsiGI ACCGGT 1 cut(s) 531
AspLEI GCGC 1 cut(s) 1068
AspS9I GGNCC 5 cut(s) 534, 1089, 1255, 1256, 1511
AsuHPI GGTGA 1 cut(s) 1033
AsuII TTCGAA 1 cut(s) 1415
AvaII GGWCC 2 cut(s) 534, 1511
BaeGI GKGCMC 1 cut(s) 1259
BamHI GGATCC 1 cut(s) 976
BanI GGYRCC 1 cut(s) 1655
BanII GRGCYC 2 cut(s) 293, 1259
BauI CACGAG 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 728
Bbv12I GWGCWC 2 cut(s) 293, 1333
BbvCI CCTCAGC 2 cut(s) 570, 1161
BccI CCATC 4 cut(s) 307, 747, 1263, 1574
BceAI ACGGC 2 cut(s) 1225, 1648
BciT130I CCWGG 2 cut(s) 556, 1093
BcoDI GTCTC 3 cut(s) 25, 239, 643
BfaI CTAG 1 cut(s) 1394
BfmI CTRYAG 2 cut(s) 42, 1380
BfrI CTTAAG 3 cut(s) 103, 499, 1429
BfuAI ACCTGC 1 cut(s) 1499
BglII AGATCT 1 cut(s) 495
Bme1390I CCNGG 2 cut(s) 556, 1093
Bme18I GGWCC 2 cut(s) 534, 1511
BmgT120I GGNCC 5 cut(s) 534, 1089, 1255, 1256, 1511
BmiI GGNNCC 8 cut(s) 536, 776, 978, 1256, 1257, 1499, 1513, 1657
BmrFI CCNGG 2 cut(s) 556, 1093
BmrI ACTGGG 1 cut(s) 799
BmsI GCATC 5 cut(s) 211, 649, 1059, 1307, 1644
BmuI ACTGGG 1 cut(s) 799
BoxI GACNNNNGTC 1 cut(s) 728
BpiI GAAGAC 1 cut(s) 728
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 2 cut(s) 246, 766
Bpu10I CCTNAGC 2 cut(s) 570, 1161
Bpu14I TTCGAA 1 cut(s) 1415
BpuEI CTTGAG 1 cut(s) 1069
BsaJI CCNNGG 3 cut(s) 778, 1091, 1092
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 219, 249, 1545, 1575
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 6 cut(s) 749, 805, 1380, 1414, 1639, 1651
BseBI CCWGG 2 cut(s) 556, 1093
BseDI CCNNGG 3 cut(s) 778, 1091, 1092
BseGI GGATG 4 cut(s) 349, 826, 1585, 1635
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMII CTCAG 5 cut(s) 192, 506, 561, 1152, 1373
BseNI ACTGG 6 cut(s) 749, 805, 1380, 1414, 1639, 1651
BseRI GAGGAG 2 cut(s) 22, 763
BseSI GKGCMC 1 cut(s) 1259
BseYI CCCAGC 1 cut(s) 1258
BsgI GTGCAG 3 cut(s) 528, 977, 1014
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 889, 1090, 1257
BshNI GGYRCC 1 cut(s) 1655
BshTI ACCGGT 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 293, 1333
BsiSI CCGG 2 cut(s) 78, 532
BslFI GGGAC 5 cut(s) 520, 1360, 1412, 1497, 1500
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 3 cut(s) 25, 239, 643
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 5 cut(s) 520, 1360, 1412, 1497, 1500
BsmI GAATGC 1 cut(s) 1008
BsnI GGCC 3 cut(s) 889, 1090, 1257
Bsp119I TTCGAA 1 cut(s) 1415
Bsp120I GGGCCC 1 cut(s) 1255
Bsp1286I GDGCHC 3 cut(s) 293, 1259, 1333
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 495, 976
BspACI CCGC 4 cut(s) 142, 207, 409, 1212
BspANI GGCC 3 cut(s) 889, 1090, 1257
BspCNI CTCAG 5 cut(s) 193, 507, 562, 1153, 1372
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 8 cut(s) 536, 776, 978, 1256, 1257, 1499, 1513, 1657
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 1 cut(s) 1499
BspPI GGATC 2 cut(s) 971, 984
BspT104I TTCGAA 1 cut(s) 1415
BspT107I GGYRCC 1 cut(s) 1655
BspTI CTTAAG 3 cut(s) 103, 499, 1429
BsrBI CCGCTC 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 6 cut(s) 749, 805, 1380, 1414, 1639, 1651
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 778, 1091, 1092
BssMI GATC 2 cut(s) 495, 976
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 778
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 556, 1093
Bst4CI ACNGT 2 cut(s) 429, 1403
BstAFI CTTAAG 3 cut(s) 103, 499, 1429
BstAPI GCANNNNNTGC 3 cut(s) 74, 1313, 1379
BstAUI TGTACA 1 cut(s) 489
BstBI TTCGAA 1 cut(s) 1415
BstC8I GCNNGC 3 cut(s) 437, 472, 948
BstDEI CTNAG 7 cut(s) 201, 515, 570, 1102, 1161, 1359, 1517
BstF5I GGATG 4 cut(s) 349, 826, 1585, 1635
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 1068
BstKTI GATC 2 cut(s) 498, 979
BstMAI GTCTC 3 cut(s) 25, 239, 643
BstMBI GATC 2 cut(s) 495, 976
BstNI CCWGG 2 cut(s) 556, 1093
BstNSI RCATGY 3 cut(s) 1000, 1316, 1493
BstPAI GACNNNNGTC 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 554, 1091
BstSFI CTRYAG 2 cut(s) 42, 1380
BstSLI GKGCMC 1 cut(s) 1259
BstUI CGCG 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 728
BstX2I RGATCY 2 cut(s) 495, 976
BstXI CCANNNNNNTGG 1 cut(s) 1646
BstYI RGATCY 2 cut(s) 495, 976
BsuRI GGCC 3 cut(s) 889, 1090, 1257
BtsCI GGATG 4 cut(s) 349, 826, 1585, 1635
BtsIMutI CAGTG 2 cut(s) 512, 1373
BveI ACCTGC 1 cut(s) 1499
Cac8I GCNNGC 3 cut(s) 437, 472, 948
CciI TCATGA 1 cut(s) 1008
CfoI GCGC 1 cut(s) 1068
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 534, 1089, 1255, 1256, 1511
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 490, 783, 1723
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 8 cut(s) 14, 116, 440, 997, 1009, 1313, 1490, 1572
CviQI GTAC 3 cut(s) 490, 783, 1723
DdeI CTNAG 7 cut(s) 201, 515, 570, 1102, 1161, 1359, 1517
DpnI GATC 2 cut(s) 497, 978
DpnII GATC 2 cut(s) 495, 976
DraIII CACNNNGTG 1 cut(s) 518
Ecl136II GAGCTC 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 2 cut(s) 293, 1259
Eco47I GGWCC 2 cut(s) 534, 1511
Eco53kI GAGCTC 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 954, 1661
EcoICRI GAGCTC 1 cut(s) 291
EcoO109I RGGNCCY 2 cut(s) 1255, 1511
EcoRII CCWGG 2 cut(s) 554, 1091
EcoT14I CCWWGG 1 cut(s) 778
EcoT38I GRGCYC 2 cut(s) 293, 1259
ErhI CCWWGG 1 cut(s) 778
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 8 cut(s) 17, 119, 443, 1000, 1012, 1316, 1493, 1575
FaqI GGGAC 5 cut(s) 520, 1360, 1412, 1497, 1500
FatI CATG 8 cut(s) 13, 115, 439, 996, 1008, 1312, 1489, 1571
FauNDI CATATG 1 cut(s) 1354
FokI GGATG 4 cut(s) 356, 813, 1592, 1622
FriOI GRGCYC 2 cut(s) 293, 1259
FspBI CTAG 1 cut(s) 1394
FspI TGCGCA 1 cut(s) 1067
GlaI GCGC 1 cut(s) 1067
GsaI CCCAGC 1 cut(s) 1262
GsuI CTGGAG 2 cut(s) 246, 766
HaeIII GGCC 3 cut(s) 889, 1090, 1257
HapII CCGG 2 cut(s) 78, 532
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 1068
Hin1II CATG 8 cut(s) 17, 119, 443, 1000, 1012, 1316, 1493, 1575
Hin6I GCGC 1 cut(s) 1066
HinP1I GCGC 1 cut(s) 1066
HincII GTYRAC 1 cut(s) 1644
HindII GTYRAC 1 cut(s) 1644
HpaI GTTAAC 1 cut(s) 1644
HpaII CCGG 2 cut(s) 78, 532
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 2 cut(s) 1285, 1644
Hpy188III TCNNGA 6 cut(s) 164, 478, 814, 830, 980, 1009
Hpy8I GTNNAC 2 cut(s) 1285, 1644
HpyAV CCTTC 6 cut(s) 21, 323, 672, 835, 939, 1454
HpyCH4III ACNGT 2 cut(s) 429, 1403
HpyF3I CTNAG 7 cut(s) 201, 515, 570, 1102, 1161, 1359, 1517
Hsp92II CATG 8 cut(s) 17, 119, 443, 1000, 1012, 1316, 1493, 1575
HspAI GCGC 1 cut(s) 1066
KspAI GTTAAC 1 cut(s) 1644
Kzo9I GATC 2 cut(s) 495, 976
LmnI GCTCC 1 cut(s) 252
LweI GCATC 5 cut(s) 211, 649, 1059, 1307, 1644
MaeI CTAG 1 cut(s) 1394
MaeIII GTNAC 1 cut(s) 1523
MalI GATC 2 cut(s) 497, 978
MbiI CCGCTC 1 cut(s) 207
MboI GATC 2 cut(s) 495, 976
MboII GAAGA 5 cut(s) 407, 733, 829, 947, 1667
MfeI CAATTG 1 cut(s) 955
MflI RGATCY 2 cut(s) 495, 976
MhlI GDGCHC 3 cut(s) 293, 1259, 1333
MlyI GAGTC 6 cut(s) 161, 191, 194, 200, 241, 248
MmeI TCCRAC 6 cut(s) 152, 168, 174, 222, 633, 1430
MseI TTAA 9 cut(s) 104, 500, 527, 654, 1364, 1430, 1466, 1614, 1643
MslI CAYNNNNRTG 3 cut(s) 1001, 1267, 1338
MspCI CTTAAG 3 cut(s) 103, 499, 1429
MspI CCGG 2 cut(s) 78, 532
MspR9I CCNGG 2 cut(s) 556, 1093
MunI CAATTG 1 cut(s) 955
Mva1269I GAATGC 1 cut(s) 1008
MvaI CCWGG 2 cut(s) 556, 1093
MvnI CGCG 1 cut(s) 144
NdeI CATATG 1 cut(s) 1354
NdeII GATC 2 cut(s) 495, 976
NlaIII CATG 8 cut(s) 17, 119, 443, 1000, 1012, 1316, 1493, 1575
NlaIV GGNNCC 8 cut(s) 536, 776, 978, 1256, 1257, 1499, 1513, 1657
NmeAIII GCCGAG 1 cut(s) 865
NmuCI GTSAC 1 cut(s) 1523
NsbI TGCGCA 1 cut(s) 1067
NspI RCATGY 3 cut(s) 1000, 1316, 1493
NspV TTCGAA 1 cut(s) 1415
OliI CACNNNNGTG 1 cut(s) 1338
PagI TCATGA 1 cut(s) 1008
PasI CCCWGGG 1 cut(s) 1092
PciI ACATGT 1 cut(s) 1489
PcsI WCGNNNNNNNCGW 1 cut(s) 1421
PctI GAATGC 1 cut(s) 1008
PfeI GAWTC 4 cut(s) 81, 94, 122, 412
PinAI ACCGGT 1 cut(s) 531
PleI GAGTC 6 cut(s) 161, 191, 193, 199, 241, 247
PpsI GAGTC 6 cut(s) 161, 191, 193, 199, 241, 247
PpuMI RGGWCCY 1 cut(s) 1511
PscI ACATGT 1 cut(s) 1489
PshAI GACNNNNGTC 1 cut(s) 728
PshBI ATTAAT 1 cut(s) 1614
PsiI TTATAA 1 cut(s) 1127
Psp124BI GAGCTC 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 1511
Psp6I CCWGG 2 cut(s) 554, 1091
PspFI CCCAGC 1 cut(s) 1258
PspGI CCWGG 2 cut(s) 554, 1091
PspN4I GGNNCC 8 cut(s) 536, 776, 978, 1256, 1257, 1499, 1513, 1657
PspOMI GGGCCC 1 cut(s) 1255
PspPI GGNCC 5 cut(s) 534, 1089, 1255, 1256, 1511
PspPPI RGGWCCY 1 cut(s) 1511
PstI CTGCAG 1 cut(s) 1384
PsuI RGATCY 2 cut(s) 495, 976
RsaI GTAC 3 cut(s) 491, 784, 1724
RsaNI GTAC 3 cut(s) 490, 783, 1723
RseI CAYNNNNRTG 3 cut(s) 1001, 1267, 1338
SacI GAGCTC 1 cut(s) 293
SaqAI TTAA 9 cut(s) 104, 500, 527, 654, 1364, 1430, 1466, 1614, 1643
Sau3AI GATC 2 cut(s) 495, 976
Sau96I GGNCC 5 cut(s) 534, 1089, 1255, 1256, 1511
SchI GAGTC 6 cut(s) 161, 191, 194, 200, 241, 248
ScrFI CCNGG 2 cut(s) 556, 1093
SduI GDGCHC 3 cut(s) 293, 1259, 1333
SfaNI GCATC 5 cut(s) 211, 649, 1059, 1307, 1644
SfcI CTRYAG 2 cut(s) 42, 1380
SfuI TTCGAA 1 cut(s) 1415
SinI GGWCC 2 cut(s) 534, 1511
SmiMI CAYNNNNRTG 3 cut(s) 1001, 1267, 1338
SmlI CTYRAG 4 cut(s) 103, 499, 1084, 1429
SmoI CTYRAG 4 cut(s) 103, 499, 1084, 1429
SsiI CCGC 4 cut(s) 142, 207, 409, 1212
SspMI CTAG 1 cut(s) 1394
SstI GAGCTC 1 cut(s) 293
StyD4I CCNGG 2 cut(s) 554, 1091
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 2 cut(s) 429, 1403
TaqI TCGA 4 cut(s) 176, 287, 401, 1415
TatI WGTACW 1 cut(s) 489
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 4 cut(s) 81, 94, 122, 412
Tru1I TTAA 9 cut(s) 104, 500, 527, 654, 1364, 1430, 1466, 1614, 1643
Tru9I TTAA 9 cut(s) 104, 500, 527, 654, 1364, 1430, 1466, 1614, 1643
TscAI CASTG 2 cut(s) 519, 1380
TseFI GTSAC 1 cut(s) 1523
Tsp45I GTSAC 1 cut(s) 1523
TspDTI ATGAA 7 cut(s) 360, 498, 641, 734, 1025, 1160, 1680
TspGWI ACGGA 1 cut(s) 1710
TspRI CASTG 2 cut(s) 519, 1380
Vha464I CTTAAG 3 cut(s) 103, 499, 1429
VpaK11BI GGWCC 2 cut(s) 534, 1511
VspI ATTAAT 1 cut(s) 1614
XapI RAATTY 4 cut(s) 159, 578, 1235, 1417
XceI RCATGY 3 cut(s) 1000, 1316, 1493
XcmI CCANNNNNNNNNTGG 1 cut(s) 1266
XspI CTAG 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.