Rh2BG681000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005445
Physical Location & Seq
Forward (+)
89056040 .. 89059340
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG681000.1

Sequence Viewer

Length: 1719 bp
ATGACGAGGAGGCATGGCGTATGGAGAAGGAGACGAGTTTCTGTAGGGTCAATTCTGATATTCGGTGCAATTTTTGTCGGAATCGGATTGCTGATTCTCAACTTAAGACCTGTCCATGATGATTCTCCAACGACAACGAGCCGCGATTTCCGTGTTGAAATTCACGACTCGGTTTCGATTTCGGAGTCGGAGTCGAAGTCGAACTCTGAGAGCGGTGCTGGTGCTGGAGTTGGGGTTGAGTCGGAGACTCGGAGCAGCGAGAGTAAGAGTAAGAGTAAGAGAAAATCGAGCTCGTGTGCTTCGGTGGAAGAGATGGGAAAGGAGTTTGAAGGAGGGTTTTGGGATGAAAGTTTCAGAGTGAGGAAACTTATTCAAAATCACTTTGAATTGAATGGTGCTTCGAGGGTGCGGAATCTTCCTCCACAACAGTTTTGCCAGCATGGTTTTGTGCTTGGTAAATCAGCAGAGGCAGGCTTCGGGAATGAAATGTACAAGATCTTAAGTGGTGCAGCACTGAGTGTGATGTTAAACCGGTCCCTGATTATTGGGCAAACCAGGGGAAAATTCCCTTTTGGGGATTACATTTCTTATTCCAACCTTTCTTTTACAATGAAAGAAATCAAGCATCTGTGGAGACTTAATAAATGTGTAAAGAAGTACGGAAGGCAACTGATTATGAGGACTGATGACTTTGAGAAACCAATGAAGACAAATGTCCTGTGTAGCAACTGGAGGAGATGGAAGCAACCTATTATATGGTTCCAAGGTACAAATGACGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATACATCCAGAGATGAGGAAGGCTGCTTCTAATTTATTTGGAAAGCCAGAGGTTCTCCGTTCTCGGCCTAATGTATTTGGAGAGCTGATGAGAGTGCTTATATCTCCTTCTGAAGATGTTGAGCAAGCAATCAATTGGATTATCGGTGGAGTGGATCCTGATATTTCTCTGCACATGCGAATGCTCATGAATAGGTCGGTGAGAGCTGCACAGGCAGCACTAAACTGCATCAGAAAAGCTGTGCGCAATCTTGGTAAAGCCTCAAGGCCCAGGGTGGTCTTAGTGTCAGATACCCCCTCTCTTATAAAAAGTATTACACTGAATATAAGCAAGTTTGCAGAGGTAATTCATTTTGATTACGAACTTTTCAAAGGAAATATATCTGATGGCGGTAAAAGATTGCGAAATTTAGAATTTAGAGCGAAAGATTGGGGCCCAGCACCCAGATGGGTCGCCTTTGTGGACTTCTTTCTTGCATCACGGGCAAAACATGCTGTTGTGTCTGGTGCTCACAGGCGTGTTGGGACTACATATGCTCAGTTAATTGCAGCACTGGCTGCAGCAAACAATCTAGGGGACAGTCCAACTGGTTCAAATTTTTCGTTCTTAAGCAGCTTTCAAAGTGATTTGTTGAGAGAAGGTTTAAGATTTCAGATTGGTTGGGGACATGTGTGGAACAGATTTGCAGGTCCCCTTAGTTGTCACAACCAGCATAACCAATGTGCTTTCACACCACTTCTCCCTCCTGCATGGTGGGATGGTCTTTGGCAATCTCCACTACCAAGGGATATTAATAGGTTGGCAGACTACGGCATCCAGTTAACTGGTTTTGGCACCATTGATGAAACACATCTTCAGTCTTTTTGTAGCTCAAAGAAAGTTGCTGTGAAAACTATTCCGTACATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

572

Amino Acids

64.25

Weight (kDa)

9.93

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 95 - 568 3.1e-264 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1112
Acc16I TGCGCA 1 cut(s) 1052
Acc36I ACCTGC 1 cut(s) 1484
AccB1I GGYRCC 1 cut(s) 1640
AccBSI CCGCTC 1 cut(s) 213
AccII CGCG 1 cut(s) 144
AciI CCGC 4 cut(s) 142, 213, 409, 1197
AclWI GGATC 2 cut(s) 956, 969
AcsI RAATTY 5 cut(s) 159, 563, 1213, 1220, 1402
AcuI CTGAAG 2 cut(s) 939, 1646
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 4 cut(s) 491, 659, 769, 1709
AfiI CCNNNNNNNGG 1 cut(s) 574
AflII CTTAAG 3 cut(s) 103, 499, 1414
AflIII ACRYGT 1 cut(s) 1474
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 9 cut(s) 158, 329, 374, 386, 391, 802, 1177, 1401, 1427
AjnI CCWGG 2 cut(s) 554, 1076
AleI CACNNNNGTG 1 cut(s) 1323
AluBI AGCT 6 cut(s) 291, 892, 1013, 1046, 1422, 1677
AluI AGCT 6 cut(s) 291, 892, 1013, 1046, 1422, 1677
Alw21I GWGCWC 2 cut(s) 293, 1318
Alw26I GTCTC 3 cut(s) 25, 239, 628
AlwI GGATC 2 cut(s) 956, 969
AoxI GGCC 3 cut(s) 872, 1073, 1240
ApaI GGGCCC 1 cut(s) 1244
ApoI RAATTY 5 cut(s) 159, 563, 1213, 1220, 1402
AseI ATTAAT 1 cut(s) 1599
AsiGI ACCGGT 1 cut(s) 531
AspLEI GCGC 1 cut(s) 1053
AspS9I GGNCC 5 cut(s) 534, 1074, 1240, 1241, 1496
AsuHPI GGTGA 1 cut(s) 1018
AvaII GGWCC 2 cut(s) 534, 1496
BaeGI GKGCMC 1 cut(s) 1244
BamHI GGATCC 1 cut(s) 961
BanI GGYRCC 1 cut(s) 1640
BanII GRGCYC 2 cut(s) 293, 1244
BauI CACGAG 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 713
Bbv12I GWGCWC 2 cut(s) 293, 1318
BccI CCATC 5 cut(s) 307, 732, 1187, 1248, 1559
BceAI ACGGC 1 cut(s) 1633
BciT130I CCWGG 2 cut(s) 556, 1078
BcoDI GTCTC 3 cut(s) 25, 239, 628
BfaI CTAG 1 cut(s) 1379
BfmI CTRYAG 2 cut(s) 42, 1365
BfrI CTTAAG 3 cut(s) 103, 499, 1414
BfuAI ACCTGC 1 cut(s) 1484
BglII AGATCT 1 cut(s) 495
Bme1390I CCNGG 2 cut(s) 556, 1078
Bme18I GGWCC 2 cut(s) 534, 1496
BmgT120I GGNCC 5 cut(s) 534, 1074, 1240, 1241, 1496
BmiI GGNNCC 7 cut(s) 536, 761, 963, 1241, 1242, 1498, 1642
BmrFI CCNGG 2 cut(s) 556, 1078
BmrI ACTGGG 1 cut(s) 784
BmsI GCATC 4 cut(s) 634, 1044, 1292, 1629
BmuI ACTGGG 1 cut(s) 784
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 713
BpmI CTGGAG 2 cut(s) 246, 751
BpuEI CTTGAG 1 cut(s) 1054
BsaJI CCNNGG 5 cut(s) 555, 763, 1076, 1077, 1589
BsaWI WCCGGW 1 cut(s) 531
BsaXI ACNNNNNCTCC 8 cut(s) 176, 182, 206, 212, 219, 249, 1530, 1560
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 6 cut(s) 734, 790, 1365, 1399, 1624, 1636
BseBI CCWGG 2 cut(s) 556, 1078
BseDI CCNNGG 5 cut(s) 555, 763, 1076, 1077, 1589
BseGI GGATG 4 cut(s) 349, 811, 1570, 1620
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 3 cut(s) 198, 506, 1358
BseNI ACTGG 6 cut(s) 734, 790, 1365, 1399, 1624, 1636
BseRI GAGGAG 2 cut(s) 22, 748
BseSI GKGCMC 1 cut(s) 1244
BseYI CCCAGC 1 cut(s) 1243
BsgI GTGCAG 3 cut(s) 528, 962, 999
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 874, 1075, 1242
BshNI GGYRCC 1 cut(s) 1640
BshTI ACCGGT 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 293, 1318
BsiSI CCGG 1 cut(s) 532
BslFI GGGAC 5 cut(s) 520, 1345, 1397, 1482, 1485
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 3 cut(s) 25, 239, 628
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 5 cut(s) 520, 1345, 1397, 1482, 1485
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 3 cut(s) 874, 1075, 1242
Bsp120I GGGCCC 1 cut(s) 1240
Bsp1286I GDGCHC 3 cut(s) 293, 1244, 1318
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 2 cut(s) 495, 961
BspACI CCGC 4 cut(s) 142, 213, 409, 1197
BspANI GGCC 3 cut(s) 874, 1075, 1242
BspCNI CTCAG 3 cut(s) 199, 507, 1357
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 7 cut(s) 536, 761, 963, 1241, 1242, 1498, 1642
BspMAI CTGCAG 1 cut(s) 1369
BspMI ACCTGC 1 cut(s) 1484
BspPI GGATC 2 cut(s) 956, 969
BspT107I GGYRCC 1 cut(s) 1640
BspTI CTTAAG 3 cut(s) 103, 499, 1414
BsrBI CCGCTC 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 6 cut(s) 734, 790, 1365, 1399, 1624, 1636
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 555, 763, 1076, 1077, 1589
BssMI GATC 2 cut(s) 495, 961
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 2 cut(s) 763, 1589
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 556, 1078
Bst4CI ACNGT 2 cut(s) 429, 1388
Bst6I CTCTTC 1 cut(s) 303
BstAFI CTTAAG 3 cut(s) 103, 499, 1414
BstAPI GCANNNNNTGC 2 cut(s) 1298, 1364
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 437, 472, 933
BstDEI CTNAG 5 cut(s) 207, 515, 1087, 1344, 1502
BstF5I GGATG 4 cut(s) 349, 811, 1570, 1620
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 1053
BstKTI GATC 2 cut(s) 498, 964
BstMAI GTCTC 3 cut(s) 25, 239, 628
BstMBI GATC 2 cut(s) 495, 961
BstMWI GCNNNNNNNGC 7 cut(s) 785, 1019, 1022, 1289, 1298, 1361, 1364
BstNI CCWGG 2 cut(s) 556, 1078
BstNSI RCATGY 3 cut(s) 985, 1301, 1478
BstPAI GACNNNNGTC 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 554, 1076
BstSFI CTRYAG 2 cut(s) 42, 1365
BstSLI GKGCMC 1 cut(s) 1244
BstUI CGCG 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 713
BstX2I RGATCY 2 cut(s) 495, 961
BstXI CCANNNNNNTGG 1 cut(s) 1631
BstYI RGATCY 2 cut(s) 495, 961
BsuRI GGCC 3 cut(s) 874, 1075, 1242
BtsCI GGATG 4 cut(s) 349, 811, 1570, 1620
BtsIMutI CAGTG 3 cut(s) 512, 1124, 1358
BveI ACCTGC 1 cut(s) 1484
Cac8I GCNNGC 3 cut(s) 437, 472, 933
CciI TCATGA 1 cut(s) 993
CfoI GCGC 1 cut(s) 1053
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 5 cut(s) 534, 1074, 1240, 1241, 1496
CseI GACGC 1 cut(s) 785
Csp6I GTAC 4 cut(s) 490, 658, 768, 1708
CspAI ACCGGT 1 cut(s) 531
CviAII CATG 8 cut(s) 14, 116, 440, 982, 994, 1298, 1475, 1557
CviQI GTAC 4 cut(s) 490, 658, 768, 1708
DdeI CTNAG 5 cut(s) 207, 515, 1087, 1344, 1502
DpnI GATC 2 cut(s) 497, 963
DpnII GATC 2 cut(s) 495, 961
DraIII CACNNNGTG 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 291
Eco130I CCWWGG 2 cut(s) 763, 1589
Eco24I GRGCYC 2 cut(s) 293, 1244
Eco47I GGWCC 2 cut(s) 534, 1496
Eco53kI GAGCTC 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 939, 1646
EcoICRI GAGCTC 1 cut(s) 291
EcoO109I RGGNCCY 2 cut(s) 1240, 1496
EcoRII CCWGG 2 cut(s) 554, 1076
EcoT14I CCWWGG 2 cut(s) 763, 1589
EcoT38I GRGCYC 2 cut(s) 293, 1244
ErhI CCWWGG 2 cut(s) 763, 1589
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 8 cut(s) 17, 119, 443, 985, 997, 1301, 1478, 1560
FaqI GGGAC 5 cut(s) 520, 1345, 1397, 1482, 1485
FatI CATG 8 cut(s) 13, 115, 439, 981, 993, 1297, 1474, 1556
FauNDI CATATG 1 cut(s) 1339
FokI GGATG 4 cut(s) 356, 798, 1577, 1607
FriOI GRGCYC 2 cut(s) 293, 1244
FspBI CTAG 1 cut(s) 1379
FspI TGCGCA 1 cut(s) 1052
GlaI GCGC 1 cut(s) 1052
GsaI CCCAGC 1 cut(s) 1247
GsuI CTGGAG 2 cut(s) 246, 751
HaeIII GGCC 3 cut(s) 874, 1075, 1242
HapII CCGG 1 cut(s) 532
HgaI GACGC 1 cut(s) 785
HhaI GCGC 1 cut(s) 1053
Hin1II CATG 8 cut(s) 17, 119, 443, 985, 997, 1301, 1478, 1560
Hin6I GCGC 1 cut(s) 1051
HinP1I GCGC 1 cut(s) 1051
HincII GTYRAC 1 cut(s) 1629
HindII GTYRAC 1 cut(s) 1629
HinfI GANTC 9 cut(s) 81, 94, 122, 167, 185, 191, 239, 247, 412
HpaI GTTAAC 1 cut(s) 1629
HpaII CCGG 1 cut(s) 532
HphI GGTGA 1 cut(s) 1018
Hpy166II GTNNAC 2 cut(s) 1270, 1629
Hpy188III TCNNGA 6 cut(s) 164, 478, 799, 815, 965, 994
Hpy8I GTNNAC 2 cut(s) 1270, 1629
HpyAV CCTTC 6 cut(s) 21, 323, 657, 820, 924, 1439
HpyCH4III ACNGT 2 cut(s) 429, 1388
HpyF10VI GCNNNNNNNGC 7 cut(s) 785, 1019, 1022, 1289, 1298, 1361, 1364
HpyF3I CTNAG 5 cut(s) 207, 515, 1087, 1344, 1502
Hsp92II CATG 8 cut(s) 17, 119, 443, 985, 997, 1301, 1478, 1560
HspAI GCGC 1 cut(s) 1051
KspAI GTTAAC 1 cut(s) 1629
Kzo9I GATC 2 cut(s) 495, 961
LmnI GCTCC 1 cut(s) 252
LweI GCATC 4 cut(s) 634, 1044, 1292, 1629
MaeI CTAG 1 cut(s) 1379
MaeIII GTNAC 1 cut(s) 1508
MalI GATC 2 cut(s) 497, 963
MbiI CCGCTC 1 cut(s) 213
MboI GATC 2 cut(s) 495, 961
MboII GAAGA 6 cut(s) 320, 407, 718, 814, 932, 1652
MfeI CAATTG 1 cut(s) 940
MflI RGATCY 2 cut(s) 495, 961
MhlI GDGCHC 3 cut(s) 293, 1244, 1318
MlyI GAGTC 5 cut(s) 161, 194, 200, 241, 248
MmeI TCCRAC 6 cut(s) 58, 152, 168, 222, 618, 1415
MseI TTAA 9 cut(s) 104, 500, 527, 639, 1349, 1415, 1451, 1599, 1628
MslI CAYNNNNRTG 3 cut(s) 986, 1252, 1323
MspCI CTTAAG 3 cut(s) 103, 499, 1414
MspI CCGG 1 cut(s) 532
MspR9I CCNGG 2 cut(s) 556, 1078
MunI CAATTG 1 cut(s) 940
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 2 cut(s) 556, 1078
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 7 cut(s) 785, 1019, 1022, 1289, 1298, 1361, 1364
NdeI CATATG 1 cut(s) 1339
NdeII GATC 2 cut(s) 495, 961
NlaIII CATG 8 cut(s) 17, 119, 443, 985, 997, 1301, 1478, 1560
NlaIV GGNNCC 7 cut(s) 536, 761, 963, 1241, 1242, 1498, 1642
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 1 cut(s) 1508
NsbI TGCGCA 1 cut(s) 1052
NspI RCATGY 3 cut(s) 985, 1301, 1478
OliI CACNNNNGTG 1 cut(s) 1323
PagI TCATGA 1 cut(s) 993
PasI CCCWGGG 1 cut(s) 1077
PciI ACATGT 1 cut(s) 1474
PctI GAATGC 1 cut(s) 993
PfeI GAWTC 4 cut(s) 81, 94, 122, 412
PinAI ACCGGT 1 cut(s) 531
PleI GAGTC 5 cut(s) 161, 193, 199, 241, 247
PpsI GAGTC 5 cut(s) 161, 193, 199, 241, 247
PpuMI RGGWCCY 1 cut(s) 1496
PscI ACATGT 1 cut(s) 1474
PshAI GACNNNNGTC 1 cut(s) 713
PshBI ATTAAT 1 cut(s) 1599
PsiI TTATAA 1 cut(s) 1112
Psp124BI GAGCTC 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 1496
Psp6I CCWGG 2 cut(s) 554, 1076
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 2 cut(s) 554, 1076
PspN4I GGNNCC 7 cut(s) 536, 761, 963, 1241, 1242, 1498, 1642
PspOMI GGGCCC 1 cut(s) 1240
PspPI GGNCC 5 cut(s) 534, 1074, 1240, 1241, 1496
PspPPI RGGWCCY 1 cut(s) 1496
PstI CTGCAG 1 cut(s) 1369
PsuI RGATCY 2 cut(s) 495, 961
RsaI GTAC 4 cut(s) 491, 659, 769, 1709
RsaNI GTAC 4 cut(s) 490, 658, 768, 1708
RseI CAYNNNNRTG 3 cut(s) 986, 1252, 1323
SacI GAGCTC 1 cut(s) 293
SaqAI TTAA 9 cut(s) 104, 500, 527, 639, 1349, 1415, 1451, 1599, 1628
Sau3AI GATC 2 cut(s) 495, 961
Sau96I GGNCC 5 cut(s) 534, 1074, 1240, 1241, 1496
SchI GAGTC 5 cut(s) 161, 194, 200, 241, 248
ScrFI CCNGG 2 cut(s) 556, 1078
SduI GDGCHC 3 cut(s) 293, 1244, 1318
SfaNI GCATC 4 cut(s) 634, 1044, 1292, 1629
SfcI CTRYAG 2 cut(s) 42, 1365
SinI GGWCC 2 cut(s) 534, 1496
SmiMI CAYNNNNRTG 3 cut(s) 986, 1252, 1323
SmlI CTYRAG 4 cut(s) 103, 499, 1069, 1414
SmoI CTYRAG 4 cut(s) 103, 499, 1069, 1414
SsiI CCGC 4 cut(s) 142, 213, 409, 1197
SspMI CTAG 1 cut(s) 1379
SstI GAGCTC 1 cut(s) 293
StyD4I CCNGG 2 cut(s) 554, 1076
StyI CCWWGG 2 cut(s) 763, 1589
TaaI ACNGT 2 cut(s) 429, 1388
TaqI TCGA 5 cut(s) 176, 194, 200, 287, 401
TatI WGTACW 1 cut(s) 489
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 4 cut(s) 81, 94, 122, 412
Tru1I TTAA 9 cut(s) 104, 500, 527, 639, 1349, 1415, 1451, 1599, 1628
Tru9I TTAA 9 cut(s) 104, 500, 527, 639, 1349, 1415, 1451, 1599, 1628
TscAI CASTG 3 cut(s) 519, 1131, 1365
TseFI GTSAC 1 cut(s) 1508
Tsp45I GTSAC 1 cut(s) 1508
TspDTI ATGAA 7 cut(s) 360, 498, 626, 719, 1010, 1145, 1665
TspGWI ACGGA 4 cut(s) 140, 675, 854, 1695
TspRI CASTG 3 cut(s) 519, 1131, 1365
Vha464I CTTAAG 3 cut(s) 103, 499, 1414
VpaK11BI GGWCC 2 cut(s) 534, 1496
VspI ATTAAT 1 cut(s) 1599
XapI RAATTY 5 cut(s) 159, 563, 1213, 1220, 1402
XceI RCATGY 3 cut(s) 985, 1301, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 1251
XspI CTAG 1 cut(s) 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.