Rroxscaffold_2G00076760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005445
Physical Location & Seq
Reverse (-)
503906 .. 506409
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076760.1

Sequence Viewer

Length: 1389 bp
ATGATGAGGAGGCCTGGCGTATGGAGAAGGAGACGAGTTTCTGTAGGGTCAATTCTGATATTCGGTGCAATTTTTGCCGGAATCGGATTGCTGATTCTCAACTTAAGACCTGTCCGTGATGATTCTCCAACGAGCCGCGATTTCCCTACTGAAATTCACGACTCGGTTTCGATTTCGGAGTCGGAGTCGGACTCTGAGAGCGGTGCTGGAGTTGGGGTTGAGTCGGAGACTCGGAGCAGCAAGAGTAAGAGTAAGAGAAAATCGAGCTCGTGTGCTTCGGTGGAAGAGATGGGAAAGGAGTTTCAAGGAGGGTTTTGGGATGAAAGTTTCAGAGTGCGGAAACTTATTCAAAATCATTTTGAATTGAATGTTTTGATTGCACTGCGGTTGAATAGAAAACTTTTGAGAGGTGAAGCTCACATGGCTAATCCAATTTCAGGTGCTTCGAGGGTGCGGAATCTTCCTCCAGAACAGTTTTGCCGGCATGGTTTTGTGCTTGGTAAATCAGCAGAGGCAGGCTTCGGGAATGAAATGTACAAGATCTTAAGTGGTGCAGCGCTGAGTGGGAAAATTCCCTTTGGGGATTACATTTCTTATTCCAACCTTTCTTTTACAATGAAAGAAATCAAGCATCTGTGGAGACTTAATAAATGTGTAAAGAAGTACGGAAGGCAACTGATTATGAGGACTGATGACTTTGAGAAACCAATGAAGACAAATGTCCTGTGTAGCAACTGGAGGAGATGGAAGCAACCTATTATATGGTTCCAAGGTACAAATGACGCTGTGGCAGCCCAGTTTTTCTTGAAGAATATACATCCAGAGATGAGGAAGGCTGCTTCTAATTTATTTGGAAAGCCAGAGGTTCTCCATTCTCGGCCTAATGTGTTTGGAGAGCTGATGAGAGTGCTTATATCTCCTTCTGAAGATGTTGAGCAAGCAATCAATTGGGTTAATGGTGGAGTGGATCCTGATATTTCTCTGCACATGCGAATGCTCATGAATAGGTCGCTGAGAGCTGCACAGGCAGCACTAAACTGCATCAGAAAAGCTGTGCGCAATCTTGGTAAAGCCTCAAGGCCCAGGGTAATTCATTTTGATTACGAACTTTTCAAAGGAAATATATCTGATGGCGGTAAAAGATTGCGAAGTCTAGAATTTAGAGCGAAAGATTGGGGCCCAGCACCCAGATGGGTCGCCTTTGTGGACTTCTTTCTTGCATCACGGGCAAAACATGCTGTTGTGTCTGGTGCTCACAGGCGTGTTGGGACTACATATGCTCAGTTAATTGCAGCACTGGCTGCAGCAAACAATCTAGGTACATGCATCCTTACAGAATATGAATTACTCGCTTATAACAAATGTTGCCAGAATTGTTCTGATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

51.97

Weight (kDa)

9.89

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7810 PF25102 87 - 123 8.8e-09 Family of unknown function (DUF7810)
DUF7810 PF25102 145 - 188 1e-19 Family of unknown function (DUF7810)
DUF7810 PF25102 189 - 364 4.7e-92 Family of unknown function (DUF7810)
DUF7810 PF25102 363 - 441 4.9e-38 Family of unknown function (DUF7810)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1356
Acc16I TGCGCA 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 1 cut(s) 138
AciI CCGC 6 cut(s) 136, 201, 337, 385, 454, 1134
AclWI GGATC 2 cut(s) 962, 975
AcsI RAATTY 3 cut(s) 153, 570, 1157
AcuI CTGAAG 1 cut(s) 945
AfaI GTAC 4 cut(s) 536, 665, 775, 1321
AfeI AGCGCT 1 cut(s) 558
AfiI CCNNNNNNNGG 1 cut(s) 437
AflII CTTAAG 2 cut(s) 103, 544
AgsI TTSAA 7 cut(s) 305, 350, 362, 367, 391, 808, 1114
AjnI CCWGG 2 cut(s) 13, 1082
AleI CACNNNNGTG 1 cut(s) 1260
AluBI AGCT 5 cut(s) 267, 416, 898, 1019, 1052
AluI AGCT 5 cut(s) 267, 416, 898, 1019, 1052
Alw21I GWGCWC 2 cut(s) 269, 1255
Alw26I GTCTC 3 cut(s) 25, 221, 634
AlwI GGATC 2 cut(s) 962, 975
Aor51HI AGCGCT 1 cut(s) 558
AoxI GGCC 4 cut(s) 11, 878, 1079, 1177
ApaI GGGCCC 1 cut(s) 1181
ApeKI GCWGC 9 cut(s) 237, 554, 791, 836, 1019, 1028, 1292, 1301, 1304
ApoI RAATTY 3 cut(s) 153, 570, 1157
AspLEI GCGC 2 cut(s) 559, 1059
AspS9I GGNCC 3 cut(s) 1080, 1177, 1178
AsuHPI GGTGA 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 1181
BamHI GGATCC 1 cut(s) 967
BanII GRGCYC 2 cut(s) 269, 1181
BauI CACGAG 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 2 cut(s) 269, 1255
BbvI GCAGC 9 cut(s) 249, 566, 803, 823, 1006, 1040, 1288, 1304, 1316
BccI CCATC 4 cut(s) 283, 738, 1124, 1185
BciT130I CCWGG 2 cut(s) 15, 1084
BcoDI GTCTC 3 cut(s) 25, 221, 634
BfaI CTAG 2 cut(s) 1154, 1316
BfmI CTRYAG 2 cut(s) 42, 1302
BfoI RGCGCY 1 cut(s) 560
BfrI CTTAAG 2 cut(s) 103, 544
BglII AGATCT 1 cut(s) 540
Bme1390I CCNGG 2 cut(s) 15, 1084
BmgT120I GGNCC 3 cut(s) 1080, 1177, 1178
BmiI GGNNCC 4 cut(s) 767, 969, 1178, 1179
BmrFI CCNGG 2 cut(s) 15, 1084
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 4 cut(s) 640, 1050, 1229, 1335
BmuI ACTGGG 1 cut(s) 790
BoxI GACNNNNGTC 1 cut(s) 719
BpiI GAAGAC 1 cut(s) 719
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 3 cut(s) 228, 450, 757
BpuEI CTTGAG 1 cut(s) 1060
BsaJI CCNNGG 3 cut(s) 769, 1082, 1083
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse118I RCCGGY 1 cut(s) 480
Bse1I ACTGG 3 cut(s) 740, 796, 1302
BseBI CCWGG 2 cut(s) 15, 1084
BseDI CCNNGG 3 cut(s) 769, 1082, 1083
BseGI GGATG 3 cut(s) 325, 817, 1326
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMII CTCAG 4 cut(s) 186, 551, 1004, 1295
BseNI ACTGG 3 cut(s) 740, 796, 1302
BseRI GAGGAG 2 cut(s) 22, 754
BseSI GKGCMC 1 cut(s) 1181
BseXI GCAGC 9 cut(s) 249, 566, 803, 823, 1006, 1040, 1288, 1304, 1316
BseYI CCCAGC 1 cut(s) 1180
BsgI GTGCAG 3 cut(s) 573, 968, 1005
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 4 cut(s) 13, 880, 1081, 1179
BsiHKAI GWGCWC 2 cut(s) 269, 1255
BsiSI CCGG 2 cut(s) 78, 481
BslFI GGGAC 1 cut(s) 1282
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 3 cut(s) 25, 221, 634
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 1282
BsmI GAATGC 1 cut(s) 999
BsnI GGCC 4 cut(s) 13, 880, 1081, 1179
Bsp120I GGGCCC 1 cut(s) 1177
Bsp1286I GDGCHC 3 cut(s) 269, 1181, 1255
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 2 cut(s) 540, 967
BspACI CCGC 6 cut(s) 136, 201, 337, 385, 454, 1134
BspANI GGCC 4 cut(s) 13, 880, 1081, 1179
BspCNI CTCAG 4 cut(s) 187, 552, 1005, 1294
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 4 cut(s) 767, 969, 1178, 1179
BspMAI CTGCAG 1 cut(s) 1306
BspPI GGATC 2 cut(s) 962, 975
BspTI CTTAAG 2 cut(s) 103, 544
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 3 cut(s) 740, 796, 1302
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 3 cut(s) 769, 1082, 1083
BssMI GATC 2 cut(s) 540, 967
BssSI CACGAG 1 cut(s) 268
BssT1I CCWWGG 1 cut(s) 769
Bst2BI CACGAG 1 cut(s) 268
Bst2UI CCWGG 2 cut(s) 15, 1084
Bst4CI ACNGT 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 279
BstAFI CTTAAG 2 cut(s) 103, 544
BstAPI GCANNNNNTGC 3 cut(s) 74, 1235, 1301
BstAUI TGTACA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 482, 517, 939
BstDEI CTNAG 4 cut(s) 195, 560, 1013, 1281
BstF5I GGATG 3 cut(s) 325, 817, 1326
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 2 cut(s) 559, 1059
BstKTI GATC 2 cut(s) 543, 970
BstMAI GTCTC 3 cut(s) 25, 221, 634
BstMBI GATC 2 cut(s) 540, 967
BstMWI GCNNNNNNNGC 9 cut(s) 74, 422, 791, 1025, 1028, 1226, 1235, 1298, 1301
BstNI CCWGG 2 cut(s) 15, 1084
BstNSI RCATGY 3 cut(s) 991, 1238, 1326
BstPAI GACNNNNGTC 1 cut(s) 719
BstSCI CCNGG 2 cut(s) 13, 1082
BstSFI CTRYAG 2 cut(s) 42, 1302
BstSLI GKGCMC 1 cut(s) 1181
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 9 cut(s) 249, 566, 803, 823, 1006, 1040, 1288, 1304, 1316
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 2 cut(s) 540, 967
BstYI RGATCY 2 cut(s) 540, 967
BsuRI GGCC 4 cut(s) 13, 880, 1081, 1179
BtsCI GGATG 3 cut(s) 325, 817, 1326
BtsI GCAGTG 1 cut(s) 380
BtsIMutI CAGTG 2 cut(s) 380, 1295
Cac8I GCNNGC 3 cut(s) 482, 517, 939
CciI TCATGA 1 cut(s) 999
CfoI GCGC 2 cut(s) 559, 1059
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 3 cut(s) 1080, 1177, 1178
CseI GACGC 1 cut(s) 791
Csp6I GTAC 4 cut(s) 535, 664, 774, 1320
CviAII CATG 6 cut(s) 421, 485, 988, 1000, 1235, 1323
CviQI GTAC 4 cut(s) 535, 664, 774, 1320
DdeI CTNAG 4 cut(s) 195, 560, 1013, 1281
DpnI GATC 2 cut(s) 542, 969
DpnII GATC 2 cut(s) 540, 967
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 769
Eco147I AGGCCT 1 cut(s) 13
Eco24I GRGCYC 2 cut(s) 269, 1181
Eco47III AGCGCT 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 945
EcoICRI GAGCTC 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 1177
EcoRII CCWGG 2 cut(s) 13, 1082
EcoT14I CCWWGG 1 cut(s) 769
EcoT22I ATGCAT 1 cut(s) 1328
EcoT38I GRGCYC 2 cut(s) 269, 1181
ErhI CCWWGG 1 cut(s) 769
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 6 cut(s) 424, 488, 991, 1003, 1238, 1326
FaqI GGGAC 1 cut(s) 1282
FatI CATG 6 cut(s) 420, 484, 987, 999, 1234, 1322
FauNDI CATATG 1 cut(s) 1276
FokI GGATG 3 cut(s) 332, 804, 1313
FriOI GRGCYC 2 cut(s) 269, 1181
FspBI CTAG 2 cut(s) 1154, 1316
FspI TGCGCA 1 cut(s) 1058
GlaI GCGC 2 cut(s) 558, 1058
GsaI CCCAGC 1 cut(s) 1184
GsuI CTGGAG 3 cut(s) 228, 450, 757
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 4 cut(s) 13, 880, 1081, 1179
HapII CCGG 2 cut(s) 78, 481
HgaI GACGC 1 cut(s) 791
HhaI GCGC 2 cut(s) 559, 1059
Hin1II CATG 6 cut(s) 424, 488, 991, 1003, 1238, 1326
Hin6I GCGC 2 cut(s) 557, 1057
HinP1I GCGC 2 cut(s) 557, 1057
HpaII CCGG 2 cut(s) 78, 481
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 1207
Hpy188III TCNNGA 8 cut(s) 158, 467, 523, 805, 821, 971, 1000, 1154
Hpy8I GTNNAC 1 cut(s) 1207
HpyAV CCTTC 4 cut(s) 21, 663, 826, 930
HpyCH4III ACNGT 1 cut(s) 474
HpyF10VI GCNNNNNNNGC 9 cut(s) 74, 422, 791, 1025, 1028, 1226, 1235, 1298, 1301
HpyF3I CTNAG 4 cut(s) 195, 560, 1013, 1281
Hsp92II CATG 6 cut(s) 424, 488, 991, 1003, 1238, 1326
HspAI GCGC 2 cut(s) 557, 1057
KroI GCCGGC 1 cut(s) 480
KroNI GCCGGC 1 cut(s) 482
Kzo9I GATC 2 cut(s) 540, 967
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 9 cut(s) 249, 566, 803, 823, 1006, 1040, 1288, 1304, 1316
LweI GCATC 4 cut(s) 640, 1050, 1229, 1335
MaeI CTAG 2 cut(s) 1154, 1316
MalI GATC 2 cut(s) 542, 969
MbiI CCGCTC 1 cut(s) 201
MboI GATC 2 cut(s) 540, 967
MboII GAAGA 5 cut(s) 296, 452, 724, 820, 938
MfeI CAATTG 1 cut(s) 946
MflI RGATCY 2 cut(s) 540, 967
MhlI GDGCHC 3 cut(s) 269, 1181, 1255
MlyI GAGTC 6 cut(s) 155, 185, 188, 194, 223, 230
MmeI TCCRAC 5 cut(s) 152, 162, 168, 204, 624
Mph1103I ATGCAT 1 cut(s) 1328
MroNI GCCGGC 1 cut(s) 480
MseI TTAA 5 cut(s) 104, 545, 645, 954, 1286
MslI CAYNNNNRTG 3 cut(s) 992, 1189, 1260
MspCI CTTAAG 2 cut(s) 103, 544
MspI CCGG 2 cut(s) 78, 481
MspR9I CCNGG 2 cut(s) 15, 1084
MunI CAATTG 1 cut(s) 946
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 2 cut(s) 15, 1084
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 9 cut(s) 74, 422, 791, 1025, 1028, 1226, 1235, 1298, 1301
NaeI GCCGGC 1 cut(s) 482
NdeI CATATG 1 cut(s) 1276
NdeII GATC 2 cut(s) 540, 967
NgoMIV GCCGGC 1 cut(s) 480
NlaIII CATG 6 cut(s) 424, 488, 991, 1003, 1238, 1326
NlaIV GGNNCC 4 cut(s) 767, 969, 1178, 1179
NmeAIII GCCGAG 1 cut(s) 856
NsbI TGCGCA 1 cut(s) 1058
NsiI ATGCAT 1 cut(s) 1328
NspI RCATGY 3 cut(s) 991, 1238, 1326
OliI CACNNNNGTG 1 cut(s) 1260
PagI TCATGA 1 cut(s) 999
PasI CCCWGGG 1 cut(s) 1083
PceI AGGCCT 1 cut(s) 13
PctI GAATGC 1 cut(s) 999
PdiI GCCGGC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 81, 94, 122, 457
PleI GAGTC 6 cut(s) 155, 185, 187, 193, 223, 229
PpsI GAGTC 6 cut(s) 155, 185, 187, 193, 223, 229
PshAI GACNNNNGTC 1 cut(s) 719
PsiI TTATAA 1 cut(s) 1356
Psp124BI GAGCTC 1 cut(s) 269
Psp6I CCWGG 2 cut(s) 13, 1082
PspFI CCCAGC 1 cut(s) 1180
PspGI CCWGG 2 cut(s) 13, 1082
PspN4I GGNNCC 4 cut(s) 767, 969, 1178, 1179
PspOMI GGGCCC 1 cut(s) 1177
PspPI GGNCC 3 cut(s) 1080, 1177, 1178
PstI CTGCAG 1 cut(s) 1306
PsuI RGATCY 2 cut(s) 540, 967
RsaI GTAC 4 cut(s) 536, 665, 775, 1321
RsaNI GTAC 4 cut(s) 535, 664, 774, 1320
RseI CAYNNNNRTG 3 cut(s) 992, 1189, 1260
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 5 cut(s) 104, 545, 645, 954, 1286
Sau3AI GATC 2 cut(s) 540, 967
Sau96I GGNCC 3 cut(s) 1080, 1177, 1178
SchI GAGTC 6 cut(s) 155, 185, 188, 194, 223, 230
ScrFI CCNGG 2 cut(s) 15, 1084
SduI GDGCHC 3 cut(s) 269, 1181, 1255
SfaNI GCATC 4 cut(s) 640, 1050, 1229, 1335
SfcI CTRYAG 2 cut(s) 42, 1302
SmiMI CAYNNNNRTG 3 cut(s) 992, 1189, 1260
SmlI CTYRAG 3 cut(s) 103, 544, 1075
SmoI CTYRAG 3 cut(s) 103, 544, 1075
SseBI AGGCCT 1 cut(s) 13
SsiI CCGC 6 cut(s) 136, 201, 337, 385, 454, 1134
SspMI CTAG 2 cut(s) 1154, 1316
SstI GAGCTC 1 cut(s) 269
StuI AGGCCT 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 13, 1082
StyI CCWWGG 1 cut(s) 769
TaaI ACNGT 1 cut(s) 474
TaqI TCGA 3 cut(s) 170, 263, 446
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 4 cut(s) 81, 94, 122, 457
Tru1I TTAA 5 cut(s) 104, 545, 645, 954, 1286
Tru9I TTAA 5 cut(s) 104, 545, 645, 954, 1286
TscAI CASTG 2 cut(s) 387, 1302
TseI GCWGC 9 cut(s) 237, 554, 791, 836, 1019, 1028, 1292, 1301, 1304
TspDTI ATGAA 7 cut(s) 336, 543, 632, 725, 1016, 1082, 1356
TspGWI ACGGA 2 cut(s) 104, 681
TspRI CASTG 2 cut(s) 387, 1302
Vha464I CTTAAG 2 cut(s) 103, 544
XapI RAATTY 3 cut(s) 153, 570, 1157
XbaI TCTAGA 1 cut(s) 1153
XceI RCATGY 3 cut(s) 991, 1238, 1326
XcmI CCANNNNNNNNNTGG 1 cut(s) 1188
XspI CTAG 2 cut(s) 1154, 1316
Zsp2I ATGCAT 1 cut(s) 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.