pycom17g00560

exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007697
Physical Location & Seq
Forward (+)
397349 .. 399793
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2445 bp
ATGGAGAAGAACCTTCCGGACAAAGACGATCAGCCCAAACCCGATGCACCGCCACCGGACTCCTCCTCCGCCGCTACAATTCCAAAGCCTAATGACGACCCCAAGTCCACCACCACCACCACCACGACTGCTACTACAAGTCCTGACGGCGATAATAAGTCCGCTGGCACTAAGCCAGCCGATGACCAAAAGCCGGCTAATCCAACGGCTGAGGTTCAGGATGACCAGAATTCCATTGCAAAGCTTGATGATCCAAGTGTAAATAACGCCGAGACCAAAGTTTATCAAGGGAACGAAGGTGATGACCCGGATTACGAGATTAAAAAGTTCAGCAGTATGAGGGTCAATAGTACCAGAGTGAAGGATTTTAAAGCTGAGAATGTTGAAGATCAGAACGATCCGCTTCCGGTACTGGATGAGGATCCAGTTCCTGAACCCGAACCCGAACCTGAACCTGAACCTGAACCGGAAGAGGAACCTAGCCTCGACAAGGTCTCGGAGGATCTCGACCACTGCATCTCGTCTCTGCCCGAAACCGAAAAACATGAAAACGATGTCGTGGACGGCCTCAACTTTCCGAATGAGCTTCCGAAGCTGGTGGGGAAGTTCTTGAATCTAGTTGAGGACGAGGTGGTGGCGAAACGCGACTCTGGCGACGCTAAGGTGAAATGGTGTCAGGAGCCTCAGAAGGACGCGTTGTTTCTGGATGCTCTGGATCGGATAGGGAGGCTGAGCAAGTACCTCTCTTTCCTCGGATTGAAATTCGAAGGGAGTCACGGTGCGTTGATCAACCACCTCGGAAGCATTCAGCACCGAGCGTTGCTGTATCTGGAAGAAGAATTCAGAATTCTTCTGGAAGAATCTAGAACCTGCACCGTCACTGACTCTGCATCCGGCGACCATAAAGACAATAAAGATCATAAGGACAGCAAAGAGCACAGTACTAAAGGCGGGAATAATAACAGCGGCAGTAATAAGCAGCAGGATCATCAAAATCAGGAAAGCAGCGGCGGCGCATTGCCGGATCAGGTTGAGTCAGGAGGAGGCGGAGATGAATCACAGGCCGATCAGTTTCCTGGATATGCTCAAGAGGTAGTGAACAATCTGAACAAGATAGCCAAAGAGATGCTATCCAGCGGATACGAAACGGAGTGTTGCGAGGTTTACATGATCTCGAGAAGGCACTTGTTCGATGAGACTCTGCACAAATTGGGACTAGAGAAGCACAGCCTCGACGACATTCAGAAAATGCACTGGGAATCGCTCGAAAGAGAAATCGTGTCATGGGTGAAGGCCTTCAAGGAATGCACCACCGTATACTTCTCCGGCGAGCGGAAGTTCGTGGAGGCGGTGTTTGCTGACTACCCGTCCATCTCGTCGAGCCTGTTCAGCAATTTGACCAAGGGGGTGATGATACAGCTTCTCAATTTCGCGGAGGGGATCGCGATATCGAAGCGGTCGGCGGAGAAGCTGTTTAAGACGCTGGACATGTACGAGGCGTTGCGGGAGGTGGTCCCCAAAATGGACAACTTGTTTCCGGCAGACTGCGTGAACGAGCTGAAGCACGAGACCTCTAACGCCCGTACCCGCCTCGGCGTGGCGGCGATTTGTATTTTCTGCGATCTGGAGAACTCGATTAAGGCTGAGACGGGTCGAAACCCGGTTCCGGGAGGGGCGGTCCATCCGCTGACGCGATACACGATGAATTACCTGAGGTACGCGTGCGAGTACAAGGACACGCTAGAATTGGTTTTCAGAGAGCACTCGAAGATCGAACGCGCCGATTCGACGAGTAGGCCCGAACGCGGCGAGTACGAGGCAGGGGAGGGATCCGGGAACACGAGTGAGGACCAATCGATGTTTTACTTGCAGCTGATGCGCGTGATGGACTTGCTTGACTCGAATCTCGAGACCAAGGCGAAGCTGTACAAGGACGTGGCGCTGAGCTCCATCTTCATGATGAACAACGGGCGATACATCTTGCAGAAAATCAAAGTGTCGGCAGAGATCAACTCGTGTATAGGCGAGACGTGGTGCCGTCGGCGGTCGTCGGATCTGAGGCAGTACCACAAGAACTACCAGAGGGAGACGTGGGGCCGGCTGCTGCAGTGCCTGAGCCACGAAGGCCTGAGCGTGAACGGGAAGGTGGCGAAGCCCGTGTTGAAGGAGAGGTTCAAGAGCTTCAACGCCTTGTTCGATGAGATCCACAAGACGCAAAGCACGTGGGTGGTGAGCGACGAGCAGATGCAGTCGGAGTTGAGGGTGTCGATATCAGCGGTGGTGATTCCGGCGTACCGATCATTCTTGGGGCGGTTCTCGCAGGTGTTTGATGCGGGGAGGCAGACGGAGAAGTATGTAAAATTCCAGCCGGAGGATATAGAGACGTACATTGAGGAGCTATTTGATGGAAACCCCAACTCCTCCATATTCAAAAAGAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04910 map04910
Pfam Domains
Protein Families
Exo70

Protein Analysis

815

Amino Acids

91.62

Weight (kDa)

4.96

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo70_C PF03081 428 - 799 1.5e-122 Exo70 exocyst complex subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2311
Acc36I ACCTGC 2 cut(s) 878, 2311
AccB1I GGYRCC 1 cut(s) 2034
AccBSI CCGCTC 1 cut(s) 1333
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
AccIII TCCGGA 1 cut(s) 16
AcsI RAATTY 5 cut(s) 229, 761, 839, 846, 2360
AcuI CTGAAG 1 cut(s) 1580
AcvI CACGTG 1 cut(s) 2223
AfiI CCNNNNNNNGG 9 cut(s) 193, 490, 1332, 1522, 1597, 1666, 1667, 1805, 2371
AflIII ACRYGT 3 cut(s) 693, 1488, 1719
AgsI TTSAA 8 cut(s) 386, 613, 760, 1300, 2164, 2176, 2185, 2431
AhdI GACNNNNNGTC 2 cut(s) 103, 1366
AjiI CACGTC 3 cut(s) 1936, 2031, 2091
AjnI CCWGG 1 cut(s) 1075
AleI CACNNNNGTG 1 cut(s) 2225
Alw21I GWGCWC 3 cut(s) 939, 1764, 1949
AlwNI CAGNNNCTG 2 cut(s) 431, 2113
Ama87I CYCGRG 2 cut(s) 1174, 1907
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 6 cut(s) 565, 1062, 1293, 1796, 2095, 2125
ApeKI GCWGC 5 cut(s) 979, 1005, 1870, 2101, 2104
ApoI RAATTY 5 cut(s) 229, 761, 839, 846, 2360
Asp700I GAANNNNTTC 1 cut(s) 1295
AspLEI GCGC 4 cut(s) 1016, 1781, 1881, 1942
AspS9I GGNCC 5 cut(s) 1513, 1678, 1797, 1849, 2095
AsuC2I CCSGG 4 cut(s) 308, 1661, 1668, 1834
AsuHPI GGTGA 6 cut(s) 311, 676, 1300, 1420, 2242, 2293
AsuII TTCGAA 1 cut(s) 765
AvaI CYCGRG 2 cut(s) 1174, 1907
AvaII GGWCC 3 cut(s) 1513, 1678, 1849
AxyI CCTNAGG 1 cut(s) 1712
BaeI ACNNNNGTAYC 2 cut(s) 1567, 1600
BamHI GGATCC 2 cut(s) 421, 1829
BanI GGYRCC 1 cut(s) 2034
BanII GRGCYC 1 cut(s) 1949
BauI CACGAG 3 cut(s) 1565, 1840, 2014
BbrPI CACGTG 1 cut(s) 2223
Bbv12I GWGCWC 3 cut(s) 939, 1764, 1949
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 5 cut(s) 991, 1017, 1882, 2088, 2091
BccI CCATC 5 cut(s) 1379, 1689, 1879, 1958, 2399
BceAI ACGGC 4 cut(s) 163, 222, 580, 2022
BciT130I CCWGG 1 cut(s) 1077
BciVI GTATCC 1 cut(s) 1133
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 4 cut(s) 308, 1661, 1668, 1834
BfaI CTAG 5 cut(s) 480, 617, 864, 1217, 1742
BfmI CTRYAG 1 cut(s) 2105
BfoI RGCGCY 1 cut(s) 1943
BfuAI ACCTGC 2 cut(s) 878, 2311
BfuI GTATCC 1 cut(s) 1133
BglI GCCNNNNNGGC 1 cut(s) 2124
BlpI GCTNAGC 2 cut(s) 731, 1943
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 5 cut(s) 308, 1077, 1661, 1668, 1834
Bme18I GGWCC 3 cut(s) 1513, 1678, 1849
BmeRI GACNNNNNGTC 2 cut(s) 103, 1366
BmeT110I CYCGRG 2 cut(s) 1174, 1907
BmgBI CACGTC 3 cut(s) 1936, 2031, 2091
BmgT120I GGNCC 5 cut(s) 1513, 1678, 1797, 1849, 2095
BmiI GGNNCC 8 cut(s) 423, 477, 681, 1515, 1665, 1831, 2036, 2096
BmrFI CCNGG 5 cut(s) 308, 1077, 1661, 1668, 1834
BmrI ACTGGG 1 cut(s) 1264
BmsI GCATC 8 cut(s) 34, 525, 697, 899, 1116, 1866, 2235, 2320
BmuI ACTGGG 1 cut(s) 1264
BplI GAGNNNNNCTC 2 cut(s) 1997, 2029
BpmI CTGGAG 1 cut(s) 1646
Bpu10I CCTNAGC 4 cut(s) 210, 660, 2114, 2129
Bpu1102I GCTNAGC 2 cut(s) 731, 1943
Bpu14I TTCGAA 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 4 cut(s) 308, 1661, 1668, 1834
Bsa29I ATCGAT 1 cut(s) 1856
BsaAI YACGTR 1 cut(s) 2223
BsaBI GATNNNNATC 1 cut(s) 420
BsaI GGTCTC 4 cut(s) 266, 499, 1562, 1904
BsaJI CCNNGG 5 cut(s) 751, 796, 1401, 1591, 1914
BsaWI WCCGGW 4 cut(s) 16, 55, 406, 466
Bsc4I CCNNNNNNNGG 9 cut(s) 193, 490, 1332, 1522, 1597, 1666, 1667, 1805, 2371
Bse118I RCCGGY 2 cut(s) 193, 2097
Bse1I ACTGG 3 cut(s) 417, 425, 1259
Bse21I CCTNAGG 1 cut(s) 1712
Bse3DI GCAATG 2 cut(s) 234, 1016
Bse8I GATNNNNATC 1 cut(s) 420
BseAI TCCGGA 1 cut(s) 16
BseBI CCWGG 1 cut(s) 1077
BseCI ATCGAT 1 cut(s) 1856
BseDI CCNNGG 5 cut(s) 751, 796, 1401, 1591, 1914
BseGI GGATG 5 cut(s) 226, 421, 712, 890, 1681
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 9 cut(s) 193, 490, 1332, 1522, 1597, 1666, 1667, 1805, 2371
BseMI GCAATG 2 cut(s) 234, 1016
BseNI ACTGG 3 cut(s) 417, 425, 1259
BseRI GAGGAG 5 cut(s) 52, 55, 1056, 2408, 2410
BseXI GCAGC 5 cut(s) 991, 1017, 1882, 2088, 2091
BsgI GTGCAG 2 cut(s) 856, 1187
Bsh1236I CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
Bsh1285I CGRYCG 2 cut(s) 1460, 2048
BshFI GGCC 6 cut(s) 567, 1064, 1295, 1798, 2097, 2127
BshNI GGYRCC 1 cut(s) 2034
BshVI ATCGAT 1 cut(s) 1856
BsiEI CGRYCG 2 cut(s) 1460, 2048
BsiHKAI GWGCWC 3 cut(s) 939, 1764, 1949
BsiHKCI CYCGRG 2 cut(s) 1174, 1907
BslFI GGGAC 2 cut(s) 1227, 1499
BslI CCNNNNNNNGG 9 cut(s) 193, 490, 1332, 1522, 1597, 1666, 1667, 1805, 2371
BsmBI CGTCTC 5 cut(s) 528, 1640, 2021, 2081, 2375
BsmFI GGGAC 2 cut(s) 1227, 1499
BsmI GAATGC 2 cut(s) 804, 1310
BsnI GGCC 6 cut(s) 567, 1064, 1295, 1798, 2097, 2127
Bso31I GGTCTC 4 cut(s) 266, 499, 1562, 1904
BsoBI CYCGRG 2 cut(s) 1174, 1907
Bsp119I TTCGAA 1 cut(s) 765
Bsp1286I GDGCHC 3 cut(s) 939, 1764, 1949
Bsp13I TCCGGA 1 cut(s) 16
Bsp1407I TGTACA 1 cut(s) 1926
Bsp1720I GCTNAGC 2 cut(s) 731, 1943
Bsp68I TCGCGA 1 cut(s) 1445
BspANI GGCC 6 cut(s) 567, 1064, 1295, 1798, 2097, 2127
BspDI ATCGAT 1 cut(s) 1856
BspEI TCCGGA 1 cut(s) 16
BspFNI CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
BspHI TCATGA 1 cut(s) 1956
BspLI GGNNCC 8 cut(s) 423, 477, 681, 1515, 1665, 1831, 2036, 2096
BspMAI CTGCAG 1 cut(s) 2109
BspMI ACCTGC 2 cut(s) 878, 2311
BspT104I TTCGAA 1 cut(s) 765
BspT107I GGYRCC 1 cut(s) 2034
BspTNI GGTCTC 4 cut(s) 266, 499, 1562, 1904
BsrBI CCGCTC 1 cut(s) 1333
BsrDI GCAATG 2 cut(s) 234, 1016
BsrFI RCCGGY 2 cut(s) 193, 2097
BsrGI TGTACA 1 cut(s) 1926
BsrI ACTGG 3 cut(s) 417, 425, 1259
BssAI RCCGGY 2 cut(s) 193, 2097
BssECI CCNNGG 5 cut(s) 751, 796, 1401, 1591, 1914
BssNAI GTATAC 1 cut(s) 1318
BssSI CACGAG 3 cut(s) 1565, 1840, 2014
BssT1I CCWWGG 2 cut(s) 1401, 1914
Bst1107I GTATAC 1 cut(s) 1318
Bst2BI CACGAG 3 cut(s) 1565, 1840, 2014
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 4 cut(s) 779, 877, 941, 1315
Bst6I CTCTTC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 1876
BstAUI TGTACA 1 cut(s) 1926
BstBAI YACGTR 1 cut(s) 2223
BstBI TTCGAA 1 cut(s) 765
BstC8I GCNNGC 6 cut(s) 166, 177, 195, 1331, 1723, 2099
BstENI CCTNNNNNAGG 1 cut(s) 488
BstF5I GGATG 5 cut(s) 226, 421, 712, 890, 1681
BstFNI CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
BstH2I RGCGCY 1 cut(s) 1943
BstHHI GCGC 4 cut(s) 1016, 1781, 1881, 1942
BstMCI CGRYCG 2 cut(s) 1460, 2048
BstMWI GCNNNNNNNGC 8 cut(s) 592, 651, 1011, 1355, 1389, 1876, 2124, 2317
BstNI CCWGG 1 cut(s) 1077
BstNSI RCATGY 1 cut(s) 1492
BstSCI CCNGG 5 cut(s) 306, 1075, 1659, 1666, 1832
BstSFI CTRYAG 1 cut(s) 2105
BstUI CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
BstV1I GCAGC 5 cut(s) 991, 1017, 1882, 2088, 2091
BstX2I RGATCY 5 cut(s) 421, 502, 1829, 2053, 2202
BstYI RGATCY 5 cut(s) 421, 502, 1829, 2053, 2202
BstZ17I GTATAC 1 cut(s) 1318
Bsu15I ATCGAT 1 cut(s) 1856
Bsu36I CCTNAGG 1 cut(s) 1712
BsuI GTATCC 1 cut(s) 1133
BsuRI GGCC 6 cut(s) 567, 1064, 1295, 1798, 2097, 2127
BsuTUI ATCGAT 1 cut(s) 1856
BtrI CACGTC 3 cut(s) 1936, 2031, 2091
BtsCI GGATG 5 cut(s) 226, 421, 712, 890, 1681
BtsI GCAGTG 2 cut(s) 511, 2114
BtsIMutI CAGTG 4 cut(s) 511, 879, 1252, 2114
BtuMI TCGCGA 1 cut(s) 1445
BveI ACCTGC 2 cut(s) 878, 2311
Cac8I GCNNGC 6 cut(s) 166, 177, 195, 1331, 1723, 2099
CaiI CAGNNNCTG 2 cut(s) 431, 2113
CciI TCATGA 1 cut(s) 1956
CfoI GCGC 4 cut(s) 1016, 1781, 1881, 1942
Cfr10I RCCGGY 2 cut(s) 193, 2097
Cfr13I GGNCC 5 cut(s) 1513, 1678, 1797, 1849, 2095
ClaI ATCGAT 1 cut(s) 1856
CseI GACGC 5 cut(s) 665, 701, 1489, 1699, 2221
CspCI CAANNNNNGTGG 2 cut(s) 2204, 2239
CviAII CATG 5 cut(s) 545, 1168, 1284, 1489, 1957
DraI TTTAAA 1 cut(s) 370
DriI GACNNNNNGTC 2 cut(s) 103, 1366
Eam1104I CTCTTC 1 cut(s) 465
Eam1105I GACNNNNNGTC 2 cut(s) 103, 1366
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 3 cut(s) 58, 1062, 1478
Ecl136II GAGCTC 1 cut(s) 1947
Eco130I CCWWGG 2 cut(s) 1401, 1914
Eco147I AGGCCT 2 cut(s) 1295, 2127
Eco24I GRGCYC 1 cut(s) 1949
Eco31I GGTCTC 4 cut(s) 266, 499, 1562, 1904
Eco32I GATATC 2 cut(s) 1449, 2271
Eco47I GGWCC 3 cut(s) 1513, 1678, 1849
Eco53kI GAGCTC 1 cut(s) 1947
Eco57I CTGAAG 1 cut(s) 1580
Eco72I CACGTG 1 cut(s) 2223
Eco81I CCTNAGG 1 cut(s) 1712
Eco88I CYCGRG 2 cut(s) 1174, 1907
EcoICRI GAGCTC 1 cut(s) 1947
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoRI GAATTC 3 cut(s) 229, 839, 846
EcoRII CCWGG 1 cut(s) 1075
EcoRV GATATC 2 cut(s) 1449, 2271
EcoT14I CCWWGG 2 cut(s) 1401, 1914
EcoT38I GRGCYC 1 cut(s) 1949
ErhI CCWWGG 2 cut(s) 1401, 1914
Esp3I CGTCTC 5 cut(s) 528, 1640, 2021, 2081, 2375
FaeI CATG 5 cut(s) 548, 1171, 1287, 1492, 1960
FaqI GGGAC 2 cut(s) 1227, 1499
FatI CATG 5 cut(s) 544, 1167, 1283, 1488, 1956
FauI CCCGC 4 cut(s) 944, 1497, 1595, 2326
FbaI TGATCA 1 cut(s) 786
FblI GTMKAC 1 cut(s) 1317
FokI GGATG 5 cut(s) 233, 428, 719, 877, 1668
FriOI GRGCYC 1 cut(s) 1949
FspBI CTAG 5 cut(s) 480, 617, 864, 1217, 1742
GlaI GCGC 4 cut(s) 1015, 1780, 1880, 1941
GsuI CTGGAG 1 cut(s) 1646
HaeII RGCGCY 1 cut(s) 1943
HaeIII GGCC 6 cut(s) 567, 1064, 1295, 1798, 2097, 2127
HgaI GACGC 5 cut(s) 665, 701, 1489, 1699, 2221
HhaI GCGC 4 cut(s) 1016, 1781, 1881, 1942
Hin1II CATG 5 cut(s) 548, 1171, 1287, 1492, 1960
Hin6I GCGC 4 cut(s) 1014, 1779, 1879, 1940
HinP1I GCGC 4 cut(s) 1014, 1779, 1879, 1940
HindIII AAGCTT 1 cut(s) 242
HphI GGTGA 6 cut(s) 311, 676, 1300, 1420, 2242, 2293
Hpy166II GTNNAC 7 cut(s) 108, 562, 1099, 1165, 1318, 1552, 2137
Hpy8I GTNNAC 7 cut(s) 108, 562, 1099, 1165, 1318, 1552, 2137
Hpy99I CGWCG 7 cut(s) 659, 1238, 1381, 1792, 2043, 2053, 2240
HpyCH4III ACNGT 4 cut(s) 779, 877, 941, 1315
HpyCH4IV ACGT 5 cut(s) 1935, 2030, 2090, 2222, 2384
HpyF10VI GCNNNNNNNGC 8 cut(s) 592, 651, 1011, 1355, 1389, 1876, 2124, 2317
HpySE526I ACGT 5 cut(s) 1935, 2030, 2090, 2222, 2384
Hsp92II CATG 5 cut(s) 548, 1171, 1287, 1492, 1960
HspAI GCGC 4 cut(s) 1014, 1779, 1879, 1940
Kpn2I TCCGGA 1 cut(s) 16
KroI GCCGGC 2 cut(s) 193, 2097
KroNI GCCGGC 2 cut(s) 195, 2099
Ksp22I TGATCA 1 cut(s) 786
LmnI GCTCC 3 cut(s) 679, 1952, 2395
Lsp1109I GCAGC 5 cut(s) 991, 1017, 1882, 2088, 2091
LweI GCATC 8 cut(s) 34, 525, 697, 899, 1116, 1866, 2235, 2320
MaeI CTAG 5 cut(s) 480, 617, 864, 1217, 1742
MaeII ACGT 5 cut(s) 1935, 2030, 2090, 2222, 2384
MaeIII GTNAC 2 cut(s) 773, 877
MbiI CCGCTC 1 cut(s) 1333
MboII GAAGA 9 cut(s) 19, 398, 482, 842, 845, 848, 869, 1780, 1945
MflI RGATCY 5 cut(s) 421, 502, 1829, 2053, 2202
MhlI GDGCHC 3 cut(s) 939, 1764, 1949
MluI ACGCGT 2 cut(s) 693, 1719
MlyI GAGTC 7 cut(s) 53, 641, 781, 878, 1043, 1192, 1892
MmeI TCCRAC 3 cut(s) 227, 2031, 2232
MroI TCCGGA 1 cut(s) 16
MroNI GCCGGC 2 cut(s) 193, 2097
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 4 cut(s) 321, 369, 1476, 1638
MslI CAYNNNNRTG 2 cut(s) 1955, 2225
MspA1I CMGCKG 7 cut(s) 164, 966, 1008, 1137, 1687, 1873, 2276
MspR9I CCNGG 5 cut(s) 308, 1077, 1661, 1668, 1834
Mva1269I GAATGC 2 cut(s) 804, 1310
MvaI CCWGG 1 cut(s) 1077
MvnI CGCG 9 cut(s) 645, 695, 1433, 1445, 1693, 1721, 1779, 1806, 1881
MwoI GCNNNNNNNGC 8 cut(s) 592, 651, 1011, 1355, 1389, 1876, 2124, 2317
NaeI GCCGGC 2 cut(s) 195, 2099
NciI CCSGG 4 cut(s) 308, 1661, 1668, 1834
NgoMIV GCCGGC 2 cut(s) 193, 2097
NlaIII CATG 5 cut(s) 548, 1171, 1287, 1492, 1960
NlaIV GGNNCC 8 cut(s) 423, 477, 681, 1515, 1665, 1831, 2036, 2096
NmeAIII GCCGAG 2 cut(s) 295, 1572
NmuCI GTSAC 2 cut(s) 773, 877
NruI TCGCGA 1 cut(s) 1445
NspI RCATGY 1 cut(s) 1492
NspV TTCGAA 1 cut(s) 765
OliI CACNNNNGTG 1 cut(s) 2225
PaeR7I CTCGAG 2 cut(s) 1174, 1907
PagI TCATGA 1 cut(s) 1956
PaqCI CACCTGC 1 cut(s) 2311
PceI AGGCCT 2 cut(s) 1295, 2127
PciI ACATGT 1 cut(s) 1488
PcsI WCGNNNNNNNCGW 4 cut(s) 1697, 1779, 2193, 2219
PctI GAATGC 2 cut(s) 804, 1310
PdiI GCCGGC 2 cut(s) 195, 2099
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 7 cut(s) 613, 860, 1055, 1259, 1784, 1903, 2284
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 3 cut(s) 1075, 1666, 1832
PleI GAGTC 7 cut(s) 53, 641, 780, 878, 1042, 1192, 1892
PmaCI CACGTG 1 cut(s) 2223
PmlI CACGTG 1 cut(s) 2223
PpsI GAGTC 7 cut(s) 53, 641, 780, 878, 1042, 1192, 1892
Ppu21I YACGTR 1 cut(s) 2223
PscI ACATGT 1 cut(s) 1488
Psp124BI GAGCTC 1 cut(s) 1949
Psp6I CCWGG 1 cut(s) 1075
PspCI CACGTG 1 cut(s) 2223
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 8 cut(s) 423, 477, 681, 1515, 1665, 1831, 2036, 2096
PspPI GGNCC 5 cut(s) 1513, 1678, 1797, 1849, 2095
PstI CTGCAG 1 cut(s) 2109
PstNI CAGNNNCTG 2 cut(s) 431, 2113
PsuI RGATCY 5 cut(s) 421, 502, 1829, 2053, 2202
PsyI GACNNNGTC 1 cut(s) 491
PvuII CAGCTG 1 cut(s) 1873
RruI TCGCGA 1 cut(s) 1445
RseI CAYNNNNRTG 2 cut(s) 1955, 2225
SacI GAGCTC 1 cut(s) 1949
SaqAI TTAA 4 cut(s) 321, 369, 1476, 1638
Sau96I GGNCC 5 cut(s) 1513, 1678, 1797, 1849, 2095
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 7 cut(s) 53, 641, 781, 878, 1043, 1192, 1892
ScrFI CCNGG 5 cut(s) 308, 1077, 1661, 1668, 1834
SduI GDGCHC 3 cut(s) 939, 1764, 1949
SfaNI GCATC 8 cut(s) 34, 525, 697, 899, 1116, 1866, 2235, 2320
SfcI CTRYAG 1 cut(s) 2105
Sfr274I CTCGAG 2 cut(s) 1174, 1907
SfuI TTCGAA 1 cut(s) 765
SinI GGWCC 3 cut(s) 1513, 1678, 1849
SlaI CTCGAG 2 cut(s) 1174, 1907
SmiMI CAYNNNNRTG 2 cut(s) 1955, 2225
SmlI CTYRAG 3 cut(s) 1086, 1174, 1907
SmoI CTYRAG 3 cut(s) 1086, 1174, 1907
SseBI AGGCCT 2 cut(s) 1295, 2127
SspMI CTAG 5 cut(s) 480, 617, 864, 1217, 1742
SstI GAGCTC 1 cut(s) 1949
StuI AGGCCT 2 cut(s) 1295, 2127
StyD4I CCNGG 5 cut(s) 306, 1075, 1659, 1666, 1832
StyI CCWWGG 2 cut(s) 1401, 1914
TaaI ACNGT 4 cut(s) 779, 877, 941, 1315
TaiI ACGT 5 cut(s) 1938, 2033, 2093, 2225, 2387
TatI WGTACW 3 cut(s) 941, 1728, 1926
TauI GCSGC 6 cut(s) 74, 969, 1011, 1014, 1604, 1809
TfiI GAWTC 7 cut(s) 613, 860, 1055, 1259, 1784, 1903, 2284
Tru1I TTAA 4 cut(s) 321, 369, 1476, 1638
Tru9I TTAA 4 cut(s) 321, 369, 1476, 1638
TscAI CASTG 4 cut(s) 518, 886, 1259, 2114
TseFI GTSAC 2 cut(s) 773, 877
TseI GCWGC 5 cut(s) 979, 1005, 1870, 2101, 2104
Tsp45I GTSAC 2 cut(s) 773, 877
TspDTI ATGAA 5 cut(s) 561, 1068, 1718, 1945, 1976
TspGWI ACGGA 2 cut(s) 1163, 2360
TspRI CASTG 4 cut(s) 518, 886, 1259, 2114
Tth111I GACNNNGTC 1 cut(s) 491
VpaK11BI GGWCC 3 cut(s) 1513, 1678, 1849
XagI CCTNNNNNAGG 1 cut(s) 488
XapI RAATTY 5 cut(s) 229, 761, 839, 846, 2360
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 1 cut(s) 1492
XhoI CTCGAG 2 cut(s) 1174, 1907
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 5 cut(s) 480, 617, 864, 1217, 1742
ZrmI AGTACT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.