pycom17g00650

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003475
Physical Location & Seq
Forward (+)
448494 .. 452968
4475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2778 bp
ATGGCGCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGAATCTGGTCGATTCACACTTCTCCGCCAAAGATGAAGCCTTCCAAGGAAACAGTGAGGAGAAGGCGCTAGCGGGGTCCCTTGACATGTCAATTGGTGATCAAGTTGCCTCAGACAGCAGCATCCCCTTGTCTCCCCAGTGGCTGTATGCTAAACCAGATTCCAAGACACTAGCAACTGGAGCATCAGGAGAATTGCATGCAAATGATTCTCTGTCTCATGGAAACTCAACTGATGCCAATTCGAGAGACATTTGGCGTGTAGATGGATCTCAGGAGAAAAAAGATTGGAAGAGGAGTGCACCTGATTTAGATAGTAGCCGCCGCTGGCGTGAAGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGAGATCGAAAGAAAGAAGATCGCCGTGTAGGTGCTAGTGTAACGAGGGATGCTACTGAGAATAGAGCAGAAGATCGCCGTGTAGGTGCGACTTCAACTAAGGATGCTGCAGAAAATAAAGTATTGTCTTCTGACCGCTGGAATGAATCCCGAAGAGATAACAAATGGTCATCAAGTTGGGGGCCTGAAGATAAGGACAAGGACTCTCGAATAGAGAAAAAGGCTGAGGTCGAGAGGGAAGATGTACATGCTGAGAAGCAGTCTTTTTCCAATAGCAACCGTGCAGCTTCTGAGCGTGATAGTGATTCTCGTGATAAATGGAGGCCACGCCATCGCATAGAAGTTCAACCAGCTGGGGCAGCCCTATATCGTGCTGCACCAGGATTTGGGATGGCTCGAGGGCGTGTGGAAAAAGTAGGATTTGCTGCTGGACGAGGGAGGTCTAACATTGGATGCCTACAGATTGGCAAACCTGTTCTTGGAAAATCTGGCCGTTTTGCTGATATGTATTGCTATCCCAGAGGAAAGCTCCTTGATATTTATCGCAAGCAAAAGAAGGATTCAACTTTTGATGTTATGCCTGATGGGATGGAGCATGTGTCACCAATAACCCAAGTGGGCTCAATTGAGCCATTGGCTTTTGTTGCACCTGATGCAGAGGAAGAGGTTGGTCTGGGAGATATATTGAAGGGAAGAATTACTAGCAGTGAAGTGGGATACAACTCGTCTGGGGACAAGAATGTATTAAATGAAGATGCTAAAGGATCTAGCAATGTCACTTTAAGCAAGGAGGAAGGACACTTTGCAGCTAACCCGGAACAAAATGTCCAATCTGCTGAAGAGTTCATTTTGAACAATTCTTTTCCAGTTACTGGCGCTGAGGTGTCACCTATTTCTGGCGTACAAACACATATGTTAAAGGAATGTGTTGCTACTGAAGGTGAGCAGAAAGTTCTGACGGTCACAGCAGCGGCAGATAGGGAGATTGGTCCTAGTAATGATGATGCTAACTCAAGAAGTTCAGGGTGCCAGGAGCAAACCTCAAGCAGTGATCATCACTATTTCAAGAGCAATGAAGACGCCCTTCTATTGGAAAGAATTGTCTCACCTGAGGATATGAGTTTGTGCTACCTTGATCCTCAAGGGAATATCCAGGGGCCTTTCCTGGGGATCGATATAATTGCATGGTTTGAGCAAGGATTTTTTGGAATTGATTTGCCAATTCGTTCATTTGATGCGCCCAATGGATCTCCTTTCCAAGAACTTGGTGAAGTGATGCCACATCTGAAAACTAAATCTGGGCCTGTCTCTGACTCTAGTTTGCCTACTCAATTAGAGCCATTGGGTGCTGTTGGAGGCAGCTTGGACGAGAGAATATCTGCTCCTAACTATGATGGGGGGTCTGCTATTCTAAACAATCAAGGCATTCAGAATGTTGTTGCAGAAGATGAGGAAATTGTATTTCCTGGAAGGTCTAAAGGCAGCGGTGATTGCCTTTTAAGGTCTTCACCTGACATTCATGGCTCATTTGCAAATCCTCCCAGCCTTCATTCCCCTGCAAATGAAGTTTCAGAAACTAACGTCCCTGATCAGCTGGACAATAAGCTGCATCCTTTTGGCCTGTTGATGTCAGAGCTCAGAGGCAACTCACTTTTAAGGCCTGCTCAATCATCTCATTCGTTTTTCGGCATGGATGATCAGGTTCCTTTTAGAGATACCTTCGTTGGAGGTGCCACTGTTGCTAATCAGGATTCCCTTGGTGCCATGATTGATCAACCTTCCTTTGTGGAGACACGGCCGGATAATTATATAAAAAATAACACCAATGTTAGTCTGGGTTCTATAGATTCTCCCCATTTACCTCACATGGGGCAAGCAAGCAATGGCTTTGACGCAGCAGAGCATATAATGTCACAGAAGTTACTAAAAGAACAGCTTCAGCAGCTGAACCATCTTTCTACTGTTGCACAAAGTGCAGGAACAGGAGTGGACCGGTTCCCTGGATTTGGTTTTTCTGACAGCAAGAACCCTAATATCCAGCCGTCATTCCATCATCCAGTGGCAGATATGGAACATATTTTTGAAATGCAGCGTCAGCAGCAGCGTCAGTTGGAGCTTCATCAGCAGCAACACCAGTTGGAGCTTCAGCAACAGCAGCGTAAGTTGGAGCGTCAGCAACAGCAGCATTGGTTGGAGCTTCAGGAACACCAGCCATCAGTTGGAGCTGCAGCACCACCGTCAATTGGAGCTTCAGCAGCAGCAGCGTCAGCTGGAGCTTCAGCAACAGCGTCACTTGCAGGATCAACTTCACCAACACCAAATGAAATTGCAGCAACAACAGAAATCTCAAGCTCAACACTTGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

926

Amino Acids

100.61

Weight (kDa)

5.16

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 513 - 547 1.3e-09 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 1434, 2138, 2168
AccB7I CCANNNNNTGG 3 cut(s) 794, 1280, 2627
AciI CCGC 7 cut(s) 69, 116, 364, 367, 544, 1379, 1893
AclWI GGATC 6 cut(s) 319, 1180, 1538, 1586, 1663, 2716
AcoI YGGCCR 2 cut(s) 898, 2204
AcuI CTGAAG 8 cut(s) 615, 1266, 1365, 2330, 2537, 2591, 2643, 2670
AcyI GRCGYC 1 cut(s) 1488
AdeI CACNNNGTG 1 cut(s) 2381
AfaI GTAC 2 cut(s) 654, 1311
AflIII ACRYGT 1 cut(s) 129
AgeI ACCGGT 1 cut(s) 2400
AgsI TTSAA 7 cut(s) 504, 755, 972, 1096, 1261, 1474, 2492
AjnI CCWGG 6 cut(s) 787, 1437, 1560, 1572, 1873, 2407
AloI GAACNNNNNNTCC 2 cut(s) 1218, 1250
Alw21I GWGCWC 2 cut(s) 346, 2046
Alw26I GTCTC 7 cut(s) 26, 180, 264, 285, 1516, 1720, 2192
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 6 cut(s) 319, 1180, 1538, 1586, 1663, 2716
AlwNI CAGNNNCTG 4 cut(s) 187, 698, 1280, 2353
Ama87I CYCGRG 1 cut(s) 804
AoxI GGCC 8 cut(s) 590, 731, 898, 1565, 1709, 2026, 2066, 2204
ApaLI GTGCAC 1 cut(s) 342
AsiGI ACCGGT 1 cut(s) 2400
Asp700I GAANNNNTTC 1 cut(s) 2343
AspLEI GCGC 4 cut(s) 7, 112, 1286, 1648
AspS9I GGNCC 6 cut(s) 120, 590, 1397, 1565, 1709, 2398
AsuC2I CCSGG 1 cut(s) 1223
AsuHPI GGTGA 9 cut(s) 152, 1002, 1287, 1361, 1506, 1688, 1907, 1908, 2709
AsuNHI GCTAGC 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 804
AvaII GGWCC 3 cut(s) 120, 1397, 2398
AxyI CCTNAGG 1 cut(s) 1518
BaeGI GKGCMC 1 cut(s) 346
BaeI ACNNNNGTAYC 2 cut(s) 2115, 2148
BanI GGYRCC 3 cut(s) 1434, 2138, 2168
BanII GRGCYC 2 cut(s) 1031, 2046
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 3 cut(s) 528, 1491, 1905
Bbv12I GWGCWC 2 cut(s) 346, 2046
BbvCI CCTCAGC 2 cut(s) 633, 1287
BccI CCATC 9 cut(s) 302, 747, 793, 986, 991, 1796, 2367, 2466, 2629
BceAI ACGGC 5 cut(s) 417, 471, 885, 2219, 2434
BcgI CGANNNNNNTGC 2 cut(s) 1571, 1605
BciT130I CCWGG 6 cut(s) 789, 1439, 1562, 1574, 1875, 2409
BciVI GTATCC 1 cut(s) 1118
BclI TGATCA 5 cut(s) 142, 1459, 1996, 2104, 2179
BcnI CCSGG 1 cut(s) 1223
BcoDI GTCTC 7 cut(s) 26, 180, 264, 285, 1516, 1720, 2192
BfaI CTAG 7 cut(s) 113, 215, 444, 1110, 1176, 1401, 1725
BfmI CTRYAG 4 cut(s) 516, 866, 2250, 2634
BfoI RGCGCY 2 cut(s) 113, 1287
BfuI GTATCC 1 cut(s) 1118
BglII AGATCT 1 cut(s) 31
Bme1390I CCNGG 7 cut(s) 789, 1223, 1439, 1562, 1574, 1875, 2409
Bme18I GGWCC 3 cut(s) 120, 1397, 2398
BmeT110I CYCGRG 1 cut(s) 804
BmgT120I GGNCC 6 cut(s) 120, 590, 1397, 1565, 1709, 2398
BmiI GGNNCC 9 cut(s) 121, 122, 591, 1436, 1566, 2112, 2140, 2170, 2405
BmrFI CCNGG 7 cut(s) 789, 1223, 1439, 1562, 1574, 1875, 2409
BmrI ACTGGG 1 cut(s) 175
BmtI GCTAGC 1 cut(s) 116
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 3 cut(s) 528, 1491, 1905
BplI GAGNNNNNCTC 4 cut(s) 921, 953, 1433, 1465
BpmI CTGGAG 2 cut(s) 243, 2700
Bpu10I CCTNAGC 2 cut(s) 633, 1287
BpuEI CTTGAG 5 cut(s) 12, 1405, 1435, 1533, 2740
BpuMI CCSGG 1 cut(s) 1223
Bsa29I ATCGAT 1 cut(s) 1581
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 1 cut(s) 1488
BsaJI CCNNGG 5 cut(s) 88, 1561, 1573, 2164, 2407
BsaWI WCCGGW 1 cut(s) 2400
BsaXI ACNNNNNCTCC 3 cut(s) 992, 1022, 2755
Bse118I RCCGGY 1 cut(s) 2400
Bse1I ACTGG 6 cut(s) 181, 226, 1274, 1285, 2465, 2542
Bse21I CCTNAGG 1 cut(s) 1518
Bse3DI GCAATG 3 cut(s) 1186, 1486, 2296
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 6 cut(s) 789, 1439, 1562, 1574, 1875, 2409
BseCI ATCGAT 1 cut(s) 1581
BseDI CCNNGG 5 cut(s) 88, 1561, 1573, 2164, 2407
BseGI GGATG 9 cut(s) 165, 463, 517, 804, 866, 1002, 2017, 2107, 2461
BseJI GATNNNNATC 1 cut(s) 948
BseMI GCAATG 3 cut(s) 1186, 1486, 2296
BseMII CTCAG 9 cut(s) 168, 329, 456, 624, 651, 690, 1278, 1509, 2059
BseNI ACTGG 6 cut(s) 181, 226, 1274, 1285, 2465, 2542
BseRI GAGGAG 4 cut(s) 26, 116, 352, 395
BseSI GKGCMC 1 cut(s) 346
BseX3I CGGCCG 1 cut(s) 2204
BseYI CCCAGC 2 cut(s) 761, 1949
BsgI GTGCAG 3 cut(s) 711, 768, 2403
Bsh1285I CGRYCG 1 cut(s) 2207
BshFI GGCC 8 cut(s) 592, 733, 900, 1567, 1711, 2028, 2068, 2206
BshNI GGYRCC 3 cut(s) 1434, 2138, 2168
BshTI ACCGGT 1 cut(s) 2400
BshVI ATCGAT 1 cut(s) 1581
BsiEI CGRYCG 1 cut(s) 2207
BsiHKAI GWGCWC 2 cut(s) 346, 2046
BsiHKCI CYCGRG 1 cut(s) 804
BsiSI CCGG 3 cut(s) 1223, 2207, 2401
BslFI GGGAC 3 cut(s) 106, 1154, 1976
BsmAI GTCTC 7 cut(s) 26, 180, 264, 285, 1516, 1720, 2192
BsmFI GGGAC 3 cut(s) 106, 1154, 1976
BsmI GAATGC 1 cut(s) 1833
BsnI GGCC 8 cut(s) 592, 733, 900, 1567, 1711, 2028, 2068, 2206
BsoBI CYCGRG 1 cut(s) 804
Bsp1286I GDGCHC 3 cut(s) 346, 1031, 2046
Bsp1407I TGTACA 1 cut(s) 652
BspACI CCGC 7 cut(s) 69, 116, 364, 367, 544, 1379, 1893
BspANI GGCC 8 cut(s) 592, 733, 900, 1567, 1711, 2028, 2068, 2206
BspCNI CTCAG 9 cut(s) 167, 328, 457, 625, 652, 691, 1279, 1510, 2058
BspDI ATCGAT 1 cut(s) 1581
BspLI GGNNCC 9 cut(s) 121, 122, 591, 1436, 1566, 2112, 2140, 2170, 2405
BspMAI CTGCAG 2 cut(s) 520, 2638
BspOI GCTAGC 1 cut(s) 116
BspPI GGATC 6 cut(s) 319, 1180, 1538, 1586, 1663, 2716
BspT107I GGYRCC 3 cut(s) 1434, 2138, 2168
BsrDI GCAATG 3 cut(s) 1186, 1486, 2296
BsrFI RCCGGY 1 cut(s) 2400
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 6 cut(s) 181, 226, 1274, 1285, 2465, 2542
BssAI RCCGGY 1 cut(s) 2400
BssECI CCNNGG 5 cut(s) 88, 1561, 1573, 2164, 2407
BssNI GRCGYC 1 cut(s) 1488
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 2 cut(s) 88, 2164
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 6 cut(s) 789, 1439, 1562, 1574, 1875, 2409
Bst4CI ACNGT 6 cut(s) 98, 689, 1369, 2146, 2371, 2646
Bst6I CTCTTC 5 cut(s) 329, 372, 556, 1065, 1242
BstACI GRCGYC 1 cut(s) 1488
BstAPI GCANNNNNTGC 2 cut(s) 1061, 2381
BstAUI TGTACA 1 cut(s) 652
BstC8I GCNNGC 7 cut(s) 114, 243, 371, 956, 2070, 2283, 2287
BstF5I GGATG 9 cut(s) 165, 463, 517, 804, 866, 1002, 2017, 2107, 2461
BstH2I RGCGCY 2 cut(s) 113, 1287
BstHHI GCGC 4 cut(s) 7, 112, 1286, 1648
BstMAI GTCTC 7 cut(s) 26, 180, 264, 285, 1516, 1720, 2192
BstMCI CGRYCG 1 cut(s) 2207
BstNI CCWGG 6 cut(s) 789, 1439, 1562, 1574, 1875, 2409
BstNSI RCATGY 4 cut(s) 133, 245, 659, 1007
BstSCI CCNGG 7 cut(s) 787, 1221, 1437, 1560, 1572, 1873, 2407
BstSFI CTRYAG 4 cut(s) 516, 866, 2250, 2634
BstSLI GKGCMC 1 cut(s) 346
BstV2I GAAGAC 3 cut(s) 528, 1491, 1905
BstX2I RGATCY 4 cut(s) 31, 311, 1172, 1655
BstXI CCANNNNNNTGG 1 cut(s) 1673
BstYI RGATCY 4 cut(s) 31, 311, 1172, 1655
BstZI CGGCCG 1 cut(s) 2204
Bsu15I ATCGAT 1 cut(s) 1581
Bsu36I CCTNAGG 1 cut(s) 1518
BsuI GTATCC 1 cut(s) 1118
BsuRI GGCC 8 cut(s) 592, 733, 900, 1567, 1711, 2028, 2068, 2206
BsuTUI ATCGAT 1 cut(s) 1581
BtgZI GCGATG 1 cut(s) 725
BtsCI GGATG 9 cut(s) 165, 463, 517, 804, 866, 1002, 2017, 2107, 2461
BtsI GCAGTG 2 cut(s) 1120, 1462
BtsIMutI CAGTG 6 cut(s) 103, 188, 1120, 1462, 2142, 2472
Cac8I GCNNGC 7 cut(s) 114, 243, 371, 956, 2070, 2283, 2287
CaiI CAGNNNCTG 4 cut(s) 187, 698, 1280, 2353
CfoI GCGC 4 cut(s) 7, 112, 1286, 1648
Cfr10I RCCGGY 1 cut(s) 2400
Cfr13I GGNCC 6 cut(s) 120, 590, 1397, 1565, 1709, 2398
ClaI ATCGAT 1 cut(s) 1581
CseI GACGC 7 cut(s) 1496, 2309, 2489, 2501, 2567, 2661, 2685
Csp6I GTAC 2 cut(s) 653, 1310
CspAI ACCGGT 1 cut(s) 2400
CviQI GTAC 2 cut(s) 653, 1310
DraIII CACNNNGTG 1 cut(s) 2381
EaeI YGGCCR 2 cut(s) 898, 2204
EagI CGGCCG 1 cut(s) 2204
Eam1104I CTCTTC 5 cut(s) 329, 372, 556, 1065, 1242
EarI CTCTTC 5 cut(s) 329, 372, 556, 1065, 1242
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 2044
EclXI CGGCCG 1 cut(s) 2204
Eco130I CCWWGG 2 cut(s) 88, 2164
Eco147I AGGCCT 1 cut(s) 2068
Eco24I GRGCYC 2 cut(s) 1031, 2046
Eco47I GGWCC 3 cut(s) 120, 1397, 2398
Eco52I CGGCCG 1 cut(s) 2204
Eco53kI GAGCTC 1 cut(s) 2044
Eco57I CTGAAG 8 cut(s) 615, 1266, 1365, 2330, 2537, 2591, 2643, 2670
Eco81I CCTNAGG 1 cut(s) 1518
Eco88I CYCGRG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 2044
EcoO109I RGGNCCY 3 cut(s) 120, 590, 1565
EcoRII CCWGG 6 cut(s) 787, 1437, 1560, 1572, 1873, 2407
EcoT14I CCWWGG 2 cut(s) 88, 2164
EcoT38I GRGCYC 2 cut(s) 1031, 2046
ErhI CCWWGG 2 cut(s) 88, 2164
FalI AAGNNNNNCTT 4 cut(s) 1476, 1508, 2328, 2360
FaqI GGGAC 3 cut(s) 106, 1154, 1976
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 1 cut(s) 1320
FbaI TGATCA 5 cut(s) 142, 1459, 1996, 2104, 2179
FokI GGATG 9 cut(s) 152, 470, 524, 811, 873, 1009, 2004, 2114, 2448
FriOI GRGCYC 2 cut(s) 1031, 2046
FspBI CTAG 7 cut(s) 113, 215, 444, 1110, 1176, 1401, 1725
GlaI GCGC 4 cut(s) 6, 111, 1285, 1647
GsaI CCCAGC 2 cut(s) 765, 1953
GsuI CTGGAG 2 cut(s) 243, 2700
HaeII RGCGCY 2 cut(s) 113, 1287
HaeIII GGCC 8 cut(s) 592, 733, 900, 1567, 1711, 2028, 2068, 2206
HapII CCGG 3 cut(s) 1223, 2207, 2401
HgaI GACGC 7 cut(s) 1496, 2309, 2489, 2501, 2567, 2661, 2685
HhaI GCGC 4 cut(s) 7, 112, 1286, 1648
Hin1I GRCGYC 1 cut(s) 1488
Hin6I GCGC 4 cut(s) 5, 110, 1284, 1646
HinP1I GCGC 4 cut(s) 5, 110, 1284, 1646
HpaII CCGG 3 cut(s) 1223, 2207, 2401
HphI GGTGA 9 cut(s) 152, 1002, 1287, 1361, 1506, 1688, 1907, 1908, 2709
Hpy166II GTNNAC 2 cut(s) 344, 2398
Hpy8I GTNNAC 2 cut(s) 344, 2398
HpyCH4III ACNGT 6 cut(s) 98, 689, 1369, 2146, 2371, 2646
HpyCH4IV ACGT 1 cut(s) 1989
HpySE526I ACGT 1 cut(s) 1989
Hsp92I GRCGYC 1 cut(s) 1488
HspAI GCGC 4 cut(s) 5, 110, 1284, 1646
KflI GGGWCCC 1 cut(s) 120
Ksp22I TGATCA 5 cut(s) 142, 1459, 1996, 2104, 2179
MaeI CTAG 7 cut(s) 113, 215, 444, 1110, 1176, 1401, 1725
MaeII ACGT 1 cut(s) 1989
MaeIII GTNAC 9 cut(s) 448, 1008, 1183, 1276, 1293, 1369, 2319, 2328, 2697
MfeI CAATTG 3 cut(s) 135, 1032, 2649
MflI RGATCY 4 cut(s) 31, 311, 1172, 1655
MhlI GDGCHC 3 cut(s) 346, 1031, 2046
MlyI GAGTC 2 cut(s) 605, 1715
MmeI TCCRAC 7 cut(s) 1741, 2113, 2499, 2526, 2553, 2580, 2608
MroXI GAANNNNTTC 1 cut(s) 2343
MseI TTAA 5 cut(s) 1154, 1190, 1325, 1907, 2063
MslI CAYNNNNRTG 1 cut(s) 246
MspA1I CMGCKG 8 cut(s) 369, 546, 761, 1379, 1893, 2002, 2353, 2678
MspI CCGG 3 cut(s) 1223, 2207, 2401
MspR9I CCNGG 7 cut(s) 789, 1223, 1439, 1562, 1574, 1875, 2409
MunI CAATTG 3 cut(s) 135, 1032, 2649
Mva1269I GAATGC 1 cut(s) 1833
MvaI CCWGG 6 cut(s) 789, 1439, 1562, 1574, 1875, 2409
NciI CCSGG 1 cut(s) 1223
NdeI CATATG 1 cut(s) 1320
NheI GCTAGC 1 cut(s) 112
NlaIV GGNNCC 9 cut(s) 121, 122, 591, 1436, 1566, 2112, 2140, 2170, 2405
NmuCI GTSAC 6 cut(s) 1008, 1183, 1293, 1369, 2319, 2697
NspI RCATGY 4 cut(s) 133, 245, 659, 1007
PaeI GCATGC 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 804
PceI AGGCCT 1 cut(s) 2068
PciI ACATGT 1 cut(s) 129
PctI GAATGC 1 cut(s) 1833
PdmI GAANNNNTTC 1 cut(s) 2343
PfeI GAWTC 9 cut(s) 46, 56, 203, 251, 554, 713, 968, 2159, 2255
PflMI CCANNNNNTGG 3 cut(s) 794, 1280, 2627
PfoI TCCNGGA 1 cut(s) 1873
PinAI ACCGGT 1 cut(s) 2400
PleI GAGTC 2 cut(s) 605, 1715
PpsI GAGTC 2 cut(s) 605, 1715
PpuMI RGGWCCY 1 cut(s) 120
PscI ACATGT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 2046
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 6 cut(s) 787, 1437, 1560, 1572, 1873, 2407
PspFI CCCAGC 2 cut(s) 761, 1949
PspGI CCWGG 6 cut(s) 787, 1437, 1560, 1572, 1873, 2407
PspN4I GGNNCC 9 cut(s) 121, 122, 591, 1436, 1566, 2112, 2140, 2170, 2405
PspPI GGNCC 6 cut(s) 120, 590, 1397, 1565, 1709, 2398
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 804
PstI CTGCAG 2 cut(s) 520, 2638
PstNI CAGNNNCTG 4 cut(s) 187, 698, 1280, 2353
PsuI RGATCY 4 cut(s) 31, 311, 1172, 1655
PvuII CAGCTG 4 cut(s) 761, 2002, 2353, 2678
RsaI GTAC 2 cut(s) 654, 1311
RsaNI GTAC 2 cut(s) 653, 1310
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 2046
SaqAI TTAA 5 cut(s) 1154, 1190, 1325, 1907, 2063
Sau96I GGNCC 6 cut(s) 120, 590, 1397, 1565, 1709, 2398
SchI GAGTC 2 cut(s) 605, 1715
ScrFI CCNGG 7 cut(s) 789, 1223, 1439, 1562, 1574, 1875, 2409
SduI GDGCHC 3 cut(s) 346, 1031, 2046
SfcI CTRYAG 4 cut(s) 516, 866, 2250, 2634
Sfr274I CTCGAG 1 cut(s) 804
SinI GGWCC 3 cut(s) 120, 1397, 2398
SlaI CTCGAG 1 cut(s) 804
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 6 cut(s) 27, 804, 1420, 1450, 1548, 2755
SmoI CTYRAG 6 cut(s) 27, 804, 1420, 1450, 1548, 2755
SphI GCATGC 1 cut(s) 245
SseBI AGGCCT 1 cut(s) 2068
SsiI CCGC 7 cut(s) 69, 116, 364, 367, 544, 1379, 1893
SspMI CTAG 7 cut(s) 113, 215, 444, 1110, 1176, 1401, 1725
SstI GAGCTC 1 cut(s) 2046
StuI AGGCCT 1 cut(s) 2068
StyD4I CCNGG 7 cut(s) 787, 1221, 1437, 1560, 1572, 1873, 2407
StyI CCWWGG 2 cut(s) 88, 2164
TaaI ACNGT 6 cut(s) 98, 689, 1369, 2146, 2371, 2646
TaiI ACGT 1 cut(s) 1992
TaqI TCGA 8 cut(s) 44, 54, 287, 415, 616, 639, 805, 1581
TatI WGTACW 1 cut(s) 652
TauI GCSGC 3 cut(s) 366, 369, 1382
TfiI GAWTC 9 cut(s) 46, 56, 203, 251, 554, 713, 968, 2159, 2255
Tru1I TTAA 5 cut(s) 1154, 1190, 1325, 1907, 2063
Tru9I TTAA 5 cut(s) 1154, 1190, 1325, 1907, 2063
TscAI CASTG 6 cut(s) 103, 188, 1120, 1462, 2149, 2472
TseFI GTSAC 6 cut(s) 1008, 1183, 1293, 1369, 2319, 2697
Tsp45I GTSAC 6 cut(s) 1008, 1183, 1293, 1369, 2319, 2697
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 6 cut(s) 103, 188, 1120, 1462, 2149, 2472
Van91I CCANNNNNTGG 3 cut(s) 794, 1280, 2627
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 3 cut(s) 120, 1397, 2398
XceI RCATGY 4 cut(s) 133, 245, 659, 1007
XcmI CCANNNNNNNNNTGG 2 cut(s) 2239, 2624
XhoI CTCGAG 1 cut(s) 804
XmnI GAANNNNTTC 1 cut(s) 2343
XspI CTAG 7 cut(s) 113, 215, 444, 1110, 1176, 1401, 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.