Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0003475

Physical Location & Seq

Genomic Coordinates Forward (+)

453076 .. 454969

Gene Structure 1894 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGACTTGCACCAGATGTCCCATTCCTCGAGGAATCGATCCATTATTAGAGCACATCATCCAAGCAAATATAGGACGCCCGGGACAGACTGATTTCTTTGACCTCATATCTCAGGCGAAGCAAGGGAATATGCACACTTCAGAGCCGCAACCTCGTCTTCAGCAGCATGAGCTGCAGGCACAGCAGTTGTCCGCAGCATTGAGAGAGCAACTGCGGATGGAAGGGGAAAGGCGTATCGGTGCGACCCGGTTTGTAGATGAAGCAAGCCAAATTGTTAGGGATCCTGTTGGTCATCACCAGGCTCAGATGGTAGGATTTAGTTCTTCAGAAAATTACCAGAAACTTCCCACCCATGAACTGCAACTAAGCCATCTCAACTGGAATCATGCCATACGGGAGCGACTGCAGGGAGGGTTCTACGAACCTAACTCTCTGGAATTTGAGAAGTCATCTGTTCCTGCTAGTTCGCTTGGGATGAACTTGGATATTCTCAGTGCACGTGGGCAGGGTCTGGATGAGTTTTATTCGTCTCATCCAGTTGGTTTGGAGCACTTGCCTTTTGGGAACAATGGACATCTGGAAGATGGCTCCACTGAAGCACTTAAACCGCATTCGCATCTTCATGCCGAGCAAACCCGCCGAGACTCAGAAGCTGCCATGGCTTTTTCTGATGCAAATATATGGTCTTCATCTGATGGTGATAAAGAACACTCAAAACAAATTTTTATAGACCTTCACCAAAAAATTGGTCGTCAATCTACACGGTTATCAGAAATTGATTATCAGCATCACCTGTCATCTTCTAGAAGCCGGGGAGAATCTGCACATCATCCATTTAGTCTTCTCCCTGATCAAGCGGTTATGAACAACTCTTTCACGGAAGGGCCCTTTACATCTAATCCCAGAGCATTTATGGAAGATTTATCAGAGTTGGAGGGAAATAATTGGAAGAAGCAGGTTGTGAAAAGCTATGAAGGCAACATGGTAGAACAAGAAGAAACATCAAGGGAACTTCATAGTAATGCCCATAGCAGGCAGAGTTCACTTAGCAGTGCTGGGGGAGGTGGGAGTTTTTACAGCTCTGAGACAGGAATGGATAAACCGCTTGGAGAAGAGGTTTCTAATGGGAGGCTGCCTTCTACTGTAACCAAAGGGTACGACAATGCTTTGCATAGACGCCGGGTTTTATCTTCCCAGGACGTTTTGTCTGAGGCAGCACTTTCTTTGCCTGTTAAACAAAGAAATCCAGCTGCAACCCAGGTCTCTGAAACGCAAGCATCTAGCAAGAATGATGCGCAATTTAGGAGGACTTCGTCTTACAGTGACGCTTCTGTGTCCGAGGCATCATTCATAGACATGCTTAAGAAACCAGTCATTTCAGAGGCTGATGCATCCAACAGAGCAGTGGCACTGGAGTCGTCTGATGGAGGTGGCCAAGCAGGGCGGACTGGCAAAAAGAAAGGGAAGAAAGGAAGACAGATTGATCCTGCTCTCCTCGGCTTTAAGGTTTCCAGCAACCGGATCTTGATGGGCGAGATCCATCGCCTTAATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GYF

Protein Analysis

519

Amino Acids

57.22

Weight (kDa)

6.19

Isoelectric Point (pI)

52.05

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003475)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g53851 FvH4_6g53851 FvH4_6g53851 FvH4_6g53851 FvH4_6g53851
malus_domestica	MD09G1007900.v1.1 MD17G1002800.v1.1
prunus_persica	Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1 Prupe.3G309100_v2.0.a1
pyrus_communis	pycom111g00620 pycom111g00630 pycom17g00650 pycom17g00660
rosa_chinensis	RchiOBHm_Chr2g0176001
rosa_laevigata	RLG00000022372
rosa_roxburghii	Rroxscaffold_2G00076860 Rroxscaffold_2G00076870
rosa_rugosa	Rorug02G0589300 Rorug02G0589400 Rorug02G0589400
rosa_samantha	Rh2AG668500 Rh2BG680000 Rh2CG642900 Rh2DG693700
rosa_wichuraiana	Rw2G054810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	27
Acc16I	TGCGCA	1 cut(s)	1296
Acc36I	ACCTGC	1 cut(s)	946
AciI	CCGC	8 cut(s)	146, 192, 214, 608, 637, 857, 1103, 1444
AclWI	GGATC	6 cut(s)	32, 275, 288, 1478, 1529, 1531
AcoI	YGGCCR	1 cut(s)	1432
AcsI	RAATTY	2 cut(s)	437, 720
AcuI	CTGAAG	4 cut(s)	123, 143, 309, 615
AcvI	CACGTG	1 cut(s)	500
AcyI	GRCGYC	2 cut(s)	76, 1177
AfaI	GTAC	1 cut(s)	1157
AfiI	CCNNNNNNNGG	2 cut(s)	1032, 1518
AflII	CTTAAG	1 cut(s)	1361
AhdI	GACNNNNNGTC	1 cut(s)	1204
AjnI	CCWGG	3 cut(s)	297, 1194, 1257
AluBI	AGCT	5 cut(s)	172, 653, 969, 1080, 1250
AluI	AGCT	5 cut(s)	172, 653, 969, 1080, 1250
Alw21I	GWGCWC	3 cut(s)	54, 499, 552
Alw26I	GTCTC	4 cut(s)	534, 636, 1079, 1267
Alw44I	GTGCAC	1 cut(s)	495
AlwI	GGATC	6 cut(s)	32, 275, 288, 1478, 1529, 1531
AlwNI	CAGNNNCTG	3 cut(s)	511, 653, 1385
Ama87I	CYCGRG	2 cut(s)	27, 79
AoxI	GGCC	2 cut(s)	884, 1432
ApaI	GGGCCC	1 cut(s)	888
ApaLI	GTGCAC	1 cut(s)	495
ApeKI	GCWGC	7 cut(s)	163, 172, 194, 653, 1132, 1214, 1250
ApoI	RAATTY	2 cut(s)	437, 720
ArsI	GACNNNNNNTTYG	4 cut(s)	668, 700, 733, 765
AspLEI	GCGC	1 cut(s)	1297
AspS9I	GGNCC	2 cut(s)	884, 885
AsuC2I	CCSGG	5 cut(s)	80, 81, 247, 812, 1181
AsuHPI	GGTGA	4 cut(s)	287, 710, 728, 782
AvaI	CYCGRG	2 cut(s)	27, 79
BaeGI	GKGCMC	2 cut(s)	499, 888
BalI	TGGCCA	1 cut(s)	1434
BamHI	GGATCC	1 cut(s)	280
BanII	GRGCYC	1 cut(s)	888
BbrPI	CACGTG	1 cut(s)	500
BbsI	GAAGAC	4 cut(s)	149, 678, 833, 1480
Bbv12I	GWGCWC	3 cut(s)	54, 499, 552
BbvI	GCAGC	7 cut(s)	159, 175, 206, 640, 1119, 1226, 1237
BccI	CCATC	8 cut(s)	211, 301, 378, 578, 689, 1418, 1522, 1548
BcgI	CGANNNNNNTGC	2 cut(s)	1398, 1432
BciT130I	CCWGG	3 cut(s)	299, 1196, 1259
BclI	TGATCA	1 cut(s)	850
BcnI	CCSGG	5 cut(s)	80, 81, 247, 812, 1181
BcoDI	GTCTC	4 cut(s)	534, 636, 1079, 1267
BfaI	CTAG	3 cut(s)	462, 804, 1281
BfmI	CTRYAG	2 cut(s)	173, 404
BfrI	CTTAAG	1 cut(s)	1361
BfuAI	ACCTGC	1 cut(s)	946
BisI	GCNGC	8 cut(s)	146, 164, 173, 195, 654, 1133, 1215, 1251
BlsI	GCNGC	8 cut(s)	147, 165, 174, 196, 655, 1134, 1216, 1252
Bme1390I	CCNGG	8 cut(s)	80, 81, 247, 299, 812, 1181, 1196, 1259
BmeRI	GACNNNNNGTC	1 cut(s)	1204
BmeT110I	CYCGRG	2 cut(s)	27, 79
BmgT120I	GGNCC	2 cut(s)	884, 885
BmiI	GGNNCC	3 cut(s)	282, 589, 886
BmrFI	CCNGG	8 cut(s)	80, 81, 247, 299, 812, 1181, 1196, 1259
BmsI	GCATC	8 cut(s)	625, 661, 796, 1282, 1286, 1352, 1378, 1400
BpiI	GAAGAC	4 cut(s)	149, 678, 833, 1480
BpmI	CTGGAG	1 cut(s)	1433
BpuMI	CCSGG	5 cut(s)	80, 81, 247, 812, 1181
Bsa29I	ATCGAT	1 cut(s)	36
BsaAI	YACGTR	1 cut(s)	500
BsaHI	GRCGYC	2 cut(s)	76, 1177
BsaI	GGTCTC	1 cut(s)	1267
BsaJI	CCNNGG	7 cut(s)	79, 657, 811, 1194, 1257, 1338, 1495
BsaWI	WCCGGW	1 cut(s)	1518
BsaXI	ACNNNNNCTCC	2 cut(s)	1101, 1131
Bsc4I	CCNNNNNNNGG	2 cut(s)	1032, 1518
Bse1I	ACTGG	5 cut(s)	383, 536, 1370, 1416, 1453
BseBI	CCWGG	3 cut(s)	299, 1196, 1259
BseCI	ATCGAT	1 cut(s)	36
BseDI	CCNNGG	7 cut(s)	79, 657, 811, 1194, 1257, 1338, 1495
BseGI	GGATG	7 cut(s)	57, 222, 480, 520, 532, 829, 1391
BseLI	CCNNNNNNNGG	2 cut(s)	1032, 1518
BseMII	CTCAG	6 cut(s)	125, 317, 505, 660, 1074, 1200
BseNI	ACTGG	5 cut(s)	383, 536, 1370, 1416, 1453
BseRI	GAGGAG	1 cut(s)	1484
BseSI	GKGCMC	2 cut(s)	499, 888
BseXI	GCAGC	7 cut(s)	159, 175, 206, 640, 1119, 1226, 1237
BseYI	CCCAGC	1 cut(s)	1055
BsgI	GTGCAG	1 cut(s)	807
BshFI	GGCC	2 cut(s)	886, 1434
BshVI	ATCGAT	1 cut(s)	36
BsiHKAI	GWGCWC	3 cut(s)	54, 499, 552
BsiHKCI	CYCGRG	2 cut(s)	27, 79
BsiSI	CCGG	5 cut(s)	80, 247, 811, 1180, 1519
BslFI	GGGAC	2 cut(s)	3, 96
BslI	CCNNNNNNNGG	2 cut(s)	1032, 1518
BsmAI	GTCTC	4 cut(s)	534, 636, 1079, 1267
BsmBI	CGTCTC	1 cut(s)	534
BsmFI	GGGAC	2 cut(s)	3, 96
BsmI	GAATGC	1 cut(s)	610
BsnI	GGCC	2 cut(s)	886, 1434
Bso31I	GGTCTC	1 cut(s)	1267
BsoBI	CYCGRG	2 cut(s)	27, 79
Bsp120I	GGGCCC	1 cut(s)	884
Bsp1286I	GDGCHC	4 cut(s)	54, 499, 552, 888
Bsp143I	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
Bsp19I	CCATGG	1 cut(s)	657
BspACI	CCGC	8 cut(s)	146, 192, 214, 608, 637, 857, 1103, 1444
BspANI	GGCC	2 cut(s)	886, 1434
BspCNI	CTCAG	6 cut(s)	124, 316, 504, 659, 1075, 1201
BspDI	ATCGAT	1 cut(s)	36
BspLI	GGNNCC	3 cut(s)	282, 589, 886
BspMAI	CTGCAG	2 cut(s)	177, 408
BspMI	ACCTGC	1 cut(s)	946
BspPI	GGATC	6 cut(s)	32, 275, 288, 1478, 1529, 1531
BspTI	CTTAAG	1 cut(s)	1361
BspTNI	GGTCTC	1 cut(s)	1267
BsrI	ACTGG	5 cut(s)	383, 536, 1370, 1416, 1453
BssECI	CCNNGG	7 cut(s)	79, 657, 811, 1194, 1257, 1338, 1495
BssMI	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
BssNI	GRCGYC	2 cut(s)	76, 1177
BssT1I	CCWWGG	1 cut(s)	657
Bst2UI	CCWGG	3 cut(s)	299, 1196, 1259
Bst4CI	ACNGT	3 cut(s)	765, 1144, 1322
Bst6I	CTCTTC	1 cut(s)	1107
BstACI	GRCGYC	2 cut(s)	76, 1177
BstAFI	CTTAAG	1 cut(s)	1361
BstAPI	GCANNNNNTGC	1 cut(s)	172
BstBAI	YACGTR	1 cut(s)	500
BstC8I	GCNNGC	4 cut(s)	177, 265, 1034, 1275
BstDEI	CTNAG	8 cut(s)	111, 303, 365, 491, 646, 1046, 1083, 1209
BstDSI	CCRYGG	1 cut(s)	657
BstF5I	GGATG	7 cut(s)	57, 222, 480, 520, 532, 829, 1391
BstHHI	GCGC	1 cut(s)	1297
BstKTI	GATC	6 cut(s)	40, 283, 853, 1486, 1524, 1539
BstMAI	GTCTC	4 cut(s)	534, 636, 1079, 1267
BstMBI	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
BstMWI	GCNNNNNNNGC	5 cut(s)	169, 172, 181, 659, 975
BstNI	CCWGG	3 cut(s)	299, 1196, 1259
BstNSI	RCATGY	1 cut(s)	1360
BstSCI	CCNGG	8 cut(s)	78, 79, 245, 297, 810, 1179, 1194, 1257
BstSFI	CTRYAG	2 cut(s)	173, 404
BstSLI	GKGCMC	2 cut(s)	499, 888
BstV1I	GCAGC	7 cut(s)	159, 175, 206, 640, 1119, 1226, 1237
BstV2I	GAAGAC	4 cut(s)	149, 678, 833, 1480
BstX2I	RGATCY	3 cut(s)	280, 1521, 1536
BstXI	CCANNNNNNTGG	1 cut(s)	746
BstYI	RGATCY	3 cut(s)	280, 1521, 1536
Bsu15I	ATCGAT	1 cut(s)	36
BsuRI	GGCC	2 cut(s)	886, 1434
BsuTUI	ATCGAT	1 cut(s)	36
BtgI	CCRYGG	1 cut(s)	657
BtgZI	GCGATG	1 cut(s)	1526
BtsCI	GGATG	7 cut(s)	57, 222, 480, 520, 532, 829, 1391
BtsI	GCAGTG	2 cut(s)	1057, 1410
BtsIMutI	CAGTG	6 cut(s)	499, 591, 1057, 1327, 1409, 1410
BveI	ACCTGC	1 cut(s)	946
Cac8I	GCNNGC	4 cut(s)	177, 265, 1034, 1275
CaiI	CAGNNNCTG	3 cut(s)	511, 653, 1385
CfoI	GCGC	1 cut(s)	1297
Cfr13I	GGNCC	2 cut(s)	884, 885
Cfr9I	CCCGGG	1 cut(s)	79
ClaI	ATCGAT	1 cut(s)	36
CseI	GACGC	3 cut(s)	84, 1185, 1334
Csp6I	GTAC	1 cut(s)	1156
CviAII	CATG	7 cut(s)	167, 353, 386, 623, 658, 982, 1357
CviQI	GTAC	1 cut(s)	1156
DdeI	CTNAG	8 cut(s)	111, 303, 365, 491, 646, 1046, 1083, 1209
DpnI	GATC	6 cut(s)	39, 282, 852, 1485, 1523, 1538
DpnII	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
DriI	GACNNNNNGTC	1 cut(s)	1204
EaeI	YGGCCR	1 cut(s)	1432
Eam1104I	CTCTTC	1 cut(s)	1107
Eam1105I	GACNNNNNGTC	1 cut(s)	1204
EarI	CTCTTC	1 cut(s)	1107
EciI	GGCGGA	1 cut(s)	1459
Eco130I	CCWWGG	1 cut(s)	657
Eco24I	GRGCYC	1 cut(s)	888
Eco31I	GGTCTC	1 cut(s)	1267
Eco57I	CTGAAG	4 cut(s)	123, 143, 309, 615
Eco72I	CACGTG	1 cut(s)	500
Eco88I	CYCGRG	2 cut(s)	27, 79
EcoO109I	RGGNCCY	2 cut(s)	884, 885
EcoRII	CCWGG	3 cut(s)	297, 1194, 1257
EcoT14I	CCWWGG	1 cut(s)	657
EcoT22I	ATGCAT	1 cut(s)	1393
EcoT38I	GRGCYC	1 cut(s)	888
ErhI	CCWWGG	1 cut(s)	657
Esp3I	CGTCTC	1 cut(s)	534
FaeI	CATG	7 cut(s)	170, 356, 389, 626, 661, 985, 1360
FaqI	GGGAC	2 cut(s)	3, 96
FatI	CATG	7 cut(s)	166, 352, 385, 622, 657, 981, 1356
FauI	CCCGC	1 cut(s)	644
FbaI	TGATCA	1 cut(s)	850
Fnu4HI	GCNGC	8 cut(s)	146, 164, 173, 195, 654, 1133, 1215, 1251
FokI	GGATG	7 cut(s)	44, 229, 487, 519, 527, 816, 1378
FriOI	GRGCYC	1 cut(s)	888
Fsp4HI	GCNGC	8 cut(s)	146, 164, 173, 195, 654, 1133, 1215, 1251
FspBI	CTAG	3 cut(s)	462, 804, 1281
FspI	TGCGCA	1 cut(s)	1296
GlaI	GCGC	1 cut(s)	1296
GluI	GCNGC	8 cut(s)	146, 164, 173, 195, 654, 1133, 1215, 1251
GsaI	CCCAGC	1 cut(s)	1059
GsuI	CTGGAG	1 cut(s)	1433
HaeIII	GGCC	2 cut(s)	886, 1434
HapII	CCGG	5 cut(s)	80, 247, 811, 1180, 1519
HgaI	GACGC	3 cut(s)	84, 1185, 1334
HhaI	GCGC	1 cut(s)	1297
Hin1I	GRCGYC	2 cut(s)	76, 1177
Hin1II	CATG	7 cut(s)	170, 356, 389, 626, 661, 985, 1360
Hin6I	GCGC	1 cut(s)	1295
HinP1I	GCGC	1 cut(s)	1295
HinfI	GANTC	5 cut(s)	33, 382, 644, 818, 1415
HpaII	CCGG	5 cut(s)	80, 247, 811, 1180, 1519
HphI	GGTGA	4 cut(s)	287, 710, 728, 782
Hpy166II	GTNNAC	2 cut(s)	497, 1043
Hpy188III	TCNNGA	5 cut(s)	434, 512, 578, 804, 1525
Hpy8I	GTNNAC	2 cut(s)	497, 1043
HpyAV	CCTTC	5 cut(s)	215, 743, 875, 968, 1146
HpyCH4III	ACNGT	3 cut(s)	765, 1144, 1322
HpyCH4IV	ACGT	2 cut(s)	499, 1200
HpyF10VI	GCNNNNNNNGC	5 cut(s)	169, 172, 181, 659, 975
HpyF3I	CTNAG	8 cut(s)	111, 303, 365, 491, 646, 1046, 1083, 1209
HpySE526I	ACGT	2 cut(s)	499, 1200
Hsp92I	GRCGYC	2 cut(s)	76, 1177
Hsp92II	CATG	7 cut(s)	170, 356, 389, 626, 661, 985, 1360
HspAI	GCGC	1 cut(s)	1295
Ksp22I	TGATCA	1 cut(s)	850
Kzo9I	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
LmnI	GCTCC	3 cut(s)	397, 547, 593
Lsp1109I	GCAGC	7 cut(s)	159, 175, 206, 640, 1119, 1226, 1237
LweI	GCATC	8 cut(s)	625, 661, 796, 1282, 1286, 1352, 1378, 1400
MaeI	CTAG	3 cut(s)	462, 804, 1281
MaeII	ACGT	2 cut(s)	499, 1200
MaeIII	GTNAC	2 cut(s)	1144, 1322
MalI	GATC	6 cut(s)	39, 282, 852, 1485, 1523, 1538
MboI	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
MflI	RGATCY	3 cut(s)	280, 1521, 1536
MhlI	GDGCHC	4 cut(s)	54, 499, 552, 888
MlsI	TGGCCA	1 cut(s)	1434
MluCI	AATT	8 cut(s)	270, 331, 437, 720, 744, 774, 943, 1298
MluNI	TGGCCA	1 cut(s)	1434
MlyI	GAGTC	2 cut(s)	638, 1424
MmeI	TCCRAC	2 cut(s)	912, 1419
Mox20I	TGGCCA	1 cut(s)	1434
Mph1103I	ATGCAT	1 cut(s)	1393
MscI	TGGCCA	1 cut(s)	1434
MseI	TTAA	5 cut(s)	603, 1233, 1362, 1503, 1548
MslI	CAYNNNNRTG	3 cut(s)	621, 1020, 1355
Msp20I	TGGCCA	1 cut(s)	1434
MspA1I	CMGCKG	1 cut(s)	1250
MspCI	CTTAAG	1 cut(s)	1361
MspI	CCGG	5 cut(s)	80, 247, 811, 1180, 1519
MspR9I	CCNGG	8 cut(s)	80, 81, 247, 299, 812, 1181, 1196, 1259
Mva1269I	GAATGC	1 cut(s)	610
MvaI	CCWGG	3 cut(s)	299, 1196, 1259
MwoI	GCNNNNNNNGC	5 cut(s)	169, 172, 181, 659, 975
NciI	CCSGG	5 cut(s)	80, 81, 247, 812, 1181
NcoI	CCATGG	1 cut(s)	657
NdeII	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
NlaIII	CATG	7 cut(s)	170, 356, 389, 626, 661, 985, 1360
NlaIV	GGNNCC	3 cut(s)	282, 589, 886
NmeAIII	GCCGAG	3 cut(s)	652, 665, 1476
NmuCI	GTSAC	1 cut(s)	1322
NsbI	TGCGCA	1 cut(s)	1296
NsiI	ATGCAT	1 cut(s)	1393
NspI	RCATGY	1 cut(s)	1360
PaeR7I	CTCGAG	1 cut(s)	27
PctI	GAATGC	1 cut(s)	610
PfeI	GAWTC	3 cut(s)	33, 382, 818
PflFI	GACNNNGTC	1 cut(s)	1312
PkrI	GCNGC	8 cut(s)	147, 165, 174, 196, 655, 1134, 1216, 1252
PleI	GAGTC	2 cut(s)	638, 1423
PmaCI	CACGTG	1 cut(s)	500
PmlI	CACGTG	1 cut(s)	500
PpsI	GAGTC	2 cut(s)	638, 1423
Ppu21I	YACGTR	1 cut(s)	500
Psp6I	CCWGG	3 cut(s)	297, 1194, 1257
PspCI	CACGTG	1 cut(s)	500
PspFI	CCCAGC	1 cut(s)	1055
PspGI	CCWGG	3 cut(s)	297, 1194, 1257
PspN4I	GGNNCC	3 cut(s)	282, 589, 886
PspOMI	GGGCCC	1 cut(s)	884
PspPI	GGNCC	2 cut(s)	884, 885
PspXI	VCTCGAGB	1 cut(s)	27
PstI	CTGCAG	2 cut(s)	177, 408
PstNI	CAGNNNCTG	3 cut(s)	511, 653, 1385
PsuI	RGATCY	3 cut(s)	280, 1521, 1536
PsyI	GACNNNGTC	1 cut(s)	1312
PvuII	CAGCTG	1 cut(s)	1250
RsaI	GTAC	1 cut(s)	1157
RsaNI	GTAC	1 cut(s)	1156
RseI	CAYNNNNRTG	3 cut(s)	621, 1020, 1355
SaqAI	TTAA	5 cut(s)	603, 1233, 1362, 1503, 1548
SatI	GCNGC	8 cut(s)	146, 164, 173, 195, 654, 1133, 1215, 1251
Sau3AI	GATC	6 cut(s)	37, 280, 850, 1483, 1521, 1536
Sau96I	GGNCC	2 cut(s)	884, 885
SchI	GAGTC	2 cut(s)	638, 1424
ScrFI	CCNGG	8 cut(s)	80, 81, 247, 299, 812, 1181, 1196, 1259
SduI	GDGCHC	4 cut(s)	54, 499, 552, 888
SfaNI	GCATC	8 cut(s)	625, 661, 796, 1282, 1286, 1352, 1378, 1400
SfcI	CTRYAG	2 cut(s)	173, 404
Sfr274I	CTCGAG	1 cut(s)	27
SlaI	CTCGAG	1 cut(s)	27
SmaI	CCCGGG	1 cut(s)	81
SmiMI	CAYNNNNRTG	3 cut(s)	621, 1020, 1355
SmlI	CTYRAG	2 cut(s)	27, 1361
SmoI	CTYRAG	2 cut(s)	27, 1361
Sse9I	AATT	8 cut(s)	270, 331, 437, 720, 744, 774, 943, 1298
SsiI	CCGC	8 cut(s)	146, 192, 214, 608, 637, 857, 1103, 1444
SspMI	CTAG	3 cut(s)	462, 804, 1281
StyD4I	CCNGG	8 cut(s)	78, 79, 245, 297, 810, 1179, 1194, 1257
StyI	CCWWGG	1 cut(s)	657
TaaI	ACNGT	3 cut(s)	765, 1144, 1322
TaiI	ACGT	2 cut(s)	502, 1203
TaqI	TCGA	2 cut(s)	28, 36
TasI	AATT	8 cut(s)	270, 331, 437, 720, 744, 774, 943, 1298
TauI	GCSGC	1 cut(s)	148
TfiI	GAWTC	3 cut(s)	33, 382, 818
Tru1I	TTAA	5 cut(s)	603, 1233, 1362, 1503, 1548
Tru9I	TTAA	5 cut(s)	603, 1233, 1362, 1503, 1548
TscAI	CASTG	6 cut(s)	499, 598, 1057, 1327, 1410, 1416
TseFI	GTSAC	1 cut(s)	1322
TseI	GCWGC	7 cut(s)	163, 172, 194, 653, 1132, 1214, 1250
Tsp45I	GTSAC	1 cut(s)	1322
TspDTI	ATGAA	9 cut(s)	273, 369, 491, 611, 678, 878, 987, 1004, 1339
TspGWI	ACGGA	1 cut(s)	893
TspMI	CCCGGG	1 cut(s)	79
TspRI	CASTG	6 cut(s)	499, 598, 1057, 1327, 1410, 1416
Tth111I	GACNNNGTC	1 cut(s)	1312
Vha464I	CTTAAG	1 cut(s)	1361
VneI	GTGCAC	1 cut(s)	495
XapI	RAATTY	2 cut(s)	437, 720
XbaI	TCTAGA	1 cut(s)	803
XceI	RCATGY	1 cut(s)	1360
XcmI	CCANNNNNNNNNTGG	2 cut(s)	910, 1402
XhoI	CTCGAG	1 cut(s)	27
XmaI	CCCGGG	1 cut(s)	79
XspI	CTAG	3 cut(s)	462, 804, 1281
Zsp2I	ATGCAT	1 cut(s)	1393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.