pycom17g06590

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005301
Physical Location & Seq
Forward (+)
4798212 .. 4799410
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGGAGGCTCGGATGGCAGAGATTTTCCGAAGCCACAGCCTGGAATATAACCTTACCGGGCTCACGGGAAGTACTCTAGATAGCCACAGGCTTATCTATTTTGCTGGGCTTCAAGGGCATGATAAGCAACATGCTCTTGTGGGTGAACTATTCCTTGGATACTTCACACAGGCAAAATACATCGGGGACAGGGAATTTCTTCTGGAAAGTGCTCAAAAGGTTGGAGTAGAAGGGGCAGCAGAGTTTCTTGAAGACCCCAACAATGGGCTCAATGAGGTCAATGAAGAACTGGAGAAGTACGCAAGAAACATCAATGGAGTCCCACATTTTTTGTTTAACAATGGAAAGCTTGCGTTAAGTGGCGGCCAACCGCCTGAGGTGTTCTTGAGAGCTTTCGAAGCAGCCACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

138

Amino Acids

15.25

Weight (kDa)

5.17

Isoelectric Point (pI)

27.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 4 - 132 4e-17 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 40
AciI CCGC 2 cut(s) 363, 371
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 194
AdeI CACNNNGTG 1 cut(s) 411
AfaI GTAC 2 cut(s) 73, 299
AfiI CCNNNNNNNGG 3 cut(s) 40, 263, 264
AgsI TTSAA 2 cut(s) 113, 251
AjnI CCWGG 1 cut(s) 39
AjuI GAANNNNNNNTTGG 2 cut(s) 138, 170
AluBI AGCT 2 cut(s) 349, 392
AluI AGCT 2 cut(s) 349, 392
Alw21I GWGCWC 1 cut(s) 214
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 2 cut(s) 236, 401
ApoI RAATTY 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 155
AsuII TTCGAA 1 cut(s) 396
AxyI CCTNAGG 1 cut(s) 375
BanII GRGCYC 2 cut(s) 63, 270
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 248
BccI CCATC 1 cut(s) 7
BciT130I CCWGG 1 cut(s) 41
BciVI GTATCC 1 cut(s) 152
BcnI CCSGG 1 cut(s) 58
BfaI CTAG 1 cut(s) 77
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 3 cut(s) 237, 364, 402
BlsI GCNGC 3 cut(s) 238, 365, 403
BmcAI AGTACT 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 41, 58
BmrFI CCNGG 2 cut(s) 41, 58
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 263, 264
Bse1I ACTGG 1 cut(s) 294
Bse21I CCTNAGG 1 cut(s) 375
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 3 cut(s) 40, 263, 264
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 248
BseYI CCCAGC 1 cut(s) 104
BshFI GGCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 2 cut(s) 200, 305
BslI CCNNNNNNNGG 3 cut(s) 40, 263, 264
BsmFI GGGAC 2 cut(s) 200, 305
BsnI GGCC 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 3 cut(s) 63, 214, 270
BspACI CCGC 2 cut(s) 363, 371
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 1 cut(s) 367
BspT104I TTCGAA 1 cut(s) 396
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 41
BstBI TTCGAA 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 1 cut(s) 18
BstMWI GCNNNNNNNGC 4 cut(s) 14, 115, 124, 398
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 39, 56
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 375
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 351
Csp6I GTAC 2 cut(s) 72, 298
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 2 cut(s) 119, 131
CviQI GTAC 2 cut(s) 72, 298
DdeI CTNAG 1 cut(s) 375
DraIII CACNNNGTG 1 cut(s) 411
EaeI YGGCCR 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 63, 270
Eco81I CCTNAGG 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 2 cut(s) 63, 270
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 2 cut(s) 122, 134
FaiI YATR 3 cut(s) 48, 120, 132
FaqI GGGAC 2 cut(s) 200, 305
FatI CATG 2 cut(s) 118, 130
Fnu4HI GCNGC 3 cut(s) 237, 364, 402
FokI GGATG 1 cut(s) 25
FriOI GRGCYC 2 cut(s) 63, 270
Fsp4HI GCNGC 3 cut(s) 237, 364, 402
FspBI CTAG 1 cut(s) 77
GluI GCNGC 3 cut(s) 237, 364, 402
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 1 cut(s) 366
HapII CCGG 1 cut(s) 57
Hin1II CATG 2 cut(s) 122, 134
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 1 cut(s) 318
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 2 cut(s) 12, 29
Hpy188III TCNNGA 4 cut(s) 77, 203, 248, 385
Hpy8I GTNNAC 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 115, 124, 398
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 2 cut(s) 122, 134
Lsp1109I GCAGC 1 cut(s) 248
MaeI CTAG 1 cut(s) 77
MboII GAAGA 3 cut(s) 191, 263, 296
MhlI GDGCHC 3 cut(s) 63, 214, 270
MluCI AATT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 2 cut(s) 268, 370
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 2 cut(s) 336, 356
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 2 cut(s) 41, 58
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 4 cut(s) 14, 115, 124, 398
NciI CCSGG 1 cut(s) 58
NlaIII CATG 2 cut(s) 122, 134
NspI RCATGY 1 cut(s) 134
NspV TTCGAA 1 cut(s) 396
PdmI GAANNNNTTC 1 cut(s) 198
PflMI CCANNNNNTGG 1 cut(s) 40
PkrI GCNGC 3 cut(s) 238, 365, 403
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 1 cut(s) 39
RsaI GTAC 2 cut(s) 73, 299
RsaNI GTAC 2 cut(s) 72, 298
SaqAI TTAA 2 cut(s) 336, 356
SatI GCNGC 3 cut(s) 237, 364, 402
ScaI AGTACT 1 cut(s) 73
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 2 cut(s) 41, 58
SduI GDGCHC 3 cut(s) 63, 214, 270
SetI ASST 6 cut(s) 54, 222, 279, 351, 381, 394
SfuI TTCGAA 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 1 cut(s) 194
SsiI CCGC 2 cut(s) 363, 371
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 39, 56
StyI CCWWGG 1 cut(s) 154
TaqI TCGA 1 cut(s) 396
TasI AATT 1 cut(s) 194
TatI WGTACW 1 cut(s) 71
TauI GCSGC 1 cut(s) 366
Tru1I TTAA 2 cut(s) 336, 356
Tru9I TTAA 2 cut(s) 336, 356
TseI GCWGC 2 cut(s) 236, 401
TspDTI ATGAA 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 40
XapI RAATTY 1 cut(s) 194
XbaI TCTAGA 1 cut(s) 76
XceI RCATGY 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 77
ZrmI AGTACT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.