pycom17g08860

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008960
Physical Location & Seq
Reverse (-)
6679614 .. 6679949
336 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGGCCCCATTCCTCGAGTTTATCAAGAAGGATTTGCAGGTTGATTTGAGCACGGACCAATTGGTTGAGAAATTGACTGATAAGATCAGGAGGTTGAAGAAGAAGTACCAGGCCGGCGAGGATGGGGAGGACCCGGTGTTTTCCAAGCCCCATGAGCTCAAGTGCCTTGATTTGTGGAAGAAGATTTGGGGTTCTGAGGGTAACGGGATTGATAGCAATGCGAAGGGCGGCAAGAGGAAGCCTCCGAGGAAGAATAATAAGGTGAACAATGATACGGTTCTGGCCTTGCCGATTTCAGAAGTGACTGAAGAAAGAGGTGCAGAAGAATGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

112

Amino Acids

12.52

Weight (kDa)

7.72

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 2 - 64 3.7e-14 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
AciI CCGC 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 107
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 1 cut(s) 157
AluI AGCT 1 cut(s) 157
Alw21I GWGCWC 2 cut(s) 53, 159
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 3 cut(s) 3, 111, 282
AspS9I GGNCC 3 cut(s) 4, 55, 130
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 2 cut(s) 55, 130
BanII GRGCYC 1 cut(s) 159
BarI GAAGNNNNNNTAC 2 cut(s) 89, 121
Bbv12I GWGCWC 2 cut(s) 53, 159
BccI CCATC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 110
BcnI CCSGG 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 2 cut(s) 110, 134
Bme18I GGWCC 2 cut(s) 55, 130
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 3 cut(s) 4, 55, 130
BmiI GGNNCC 2 cut(s) 6, 132
BmrFI CCNGG 2 cut(s) 110, 134
BplI GAGNNNNNCTC 2 cut(s) 226, 258
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 186
BshFI GGCC 3 cut(s) 5, 113, 284
BsiHKAI GWGCWC 2 cut(s) 53, 159
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 2 cut(s) 114, 134
BsnI GGCC 3 cut(s) 5, 113, 284
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 53, 159
Bsp143I GATC 1 cut(s) 84
BspACI CCGC 1 cut(s) 228
BspANI GGCC 3 cut(s) 5, 113, 284
BspCNI CTCAG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 6, 132
BspMI ACCTGC 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 277
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 1 cut(s) 87
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 2 cut(s) 108, 132
BsuRI GGCC 3 cut(s) 5, 113, 284
BtsCI GGATG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 3 cut(s) 4, 55, 130
Csp6I GTAC 1 cut(s) 106
CviAII CATG 1 cut(s) 152
CviJI RGCY 6 cut(s) 5, 113, 148, 157, 241, 284
CviKI_1 RGCY 6 cut(s) 5, 113, 148, 157, 241, 284
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 55, 130
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 1 cut(s) 155
FaiI YATR 1 cut(s) 153
FatI CATG 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 229
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 229
GluI GCNGC 1 cut(s) 229
HaeIII GGCC 3 cut(s) 5, 113, 284
HapII CCGG 2 cut(s) 114, 134
Hin1II CATG 1 cut(s) 155
HpaII CCGG 2 cut(s) 114, 134
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 3 cut(s) 196, 246, 298
Hpy188III TCNNGA 2 cut(s) 25, 88
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 22, 217
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 2 cut(s) 37, 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 1 cut(s) 155
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 84
LpnPI CCDG 7 cut(s) 23, 73, 95, 122, 127, 147, 266
MaeIII GTNAC 2 cut(s) 200, 301
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 7 cut(s) 109, 112, 190, 193, 262, 320, 335
MfeI CAATTG 1 cut(s) 59
MhlI GDGCHC 2 cut(s) 53, 159
MluCI AATT 2 cut(s) 59, 71
MnlI CCTC 9 cut(s) 23, 84, 112, 121, 190, 228, 240, 252, 308
MroNI GCCGGC 1 cut(s) 113
MspI CCGG 2 cut(s) 114, 134
MspR9I CCNGG 2 cut(s) 110, 134
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 154
NaeI GCCGGC 1 cut(s) 115
NciI CCSGG 1 cut(s) 134
NdeII GATC 1 cut(s) 84
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 1 cut(s) 155
NlaIV GGNNCC 2 cut(s) 6, 132
NmuCI GTSAC 1 cut(s) 301
PaeR7I CTCGAG 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 115
PkrI GCNGC 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 2 cut(s) 6, 132
PspPI GGNCC 3 cut(s) 4, 55, 130
PspPPI RGGWCCY 1 cut(s) 130
PspXI VCTCGAGB 1 cut(s) 14
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SacI GAGCTC 1 cut(s) 159
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 3 cut(s) 4, 55, 130
ScrFI CCNGG 2 cut(s) 110, 134
SduI GDGCHC 2 cut(s) 53, 159
SetI ASST 5 cut(s) 42, 95, 159, 264, 319
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 2 cut(s) 55, 130
SlaI CTCGAG 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 14, 158
SmoI CTYRAG 2 cut(s) 14, 158
Sse9I AATT 2 cut(s) 59, 71
SsiI CCGC 1 cut(s) 228
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 108, 132
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 1 cut(s) 15
TasI AATT 2 cut(s) 59, 71
TauI GCSGC 1 cut(s) 231
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspGWI ACGGA 1 cut(s) 68
VpaK11BI GGWCC 2 cut(s) 55, 130
XhoI CTCGAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.