pycom17g18370

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013234
Physical Location & Seq
Forward (+)
15756685 .. 15757804
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGAAAGTTCAAAGATTTTTCCACCTTTTTGTCTTCTTTTCAGATTTTTGCAGAGATTTCTCTGCCTTTTATTTTTTCTCTCTTAAAACGCCATTTCAGATCCCCAATAAAATTTTGGTTTTTCAGTTCCCACGATCCAATTTGGACTCAAAATCTCAACAAAACCCTCCAAATTCCACAAACTTTGGTTTTATATTTCGCCTCTTCTATTTCACACTTTCATCACAGAAAAGTGTTAAAATTTCAAACTCTGAATTCGCTTCTGAAAAACAAAACCCAGATCACCAAATCTTCCATATTTCCCGAAGTTTGGTCAAAAATAGAAACTTGGGGTCTCACAAAATGCAGGAACCTGCAACTTTCCAACCCATGAAGCCCCAGTTTCCAGAGCAAGAGCAGCTGAAATGTCCGCGCTGCGAGTCCTCCAACACCAAGTTTTGCTACTACAACAACTACAACCTCTCGCAGCCGCGCCACTTCTGCAAGAACTGCAAGCGGTACTGGACCAAAGGCGGCGCTTTGAGAAATATCCCCGTCGGCGGCGGAAGCAGGAAAAACACGAAGAAGTCGTCGTCGTCAGCTTCGAAACGTTCCTCATCGACTTCCTCATCGTCACTGTCGAGCTCTTCAGCAGCAGCAGCTCAGAACCCGGATCCGCAGCCCGACCGGACCCGGGCCTACGGCTCAAAAGTCGATCAAGACCGCGGAGTGATGGACTTCTCAGGGAGCTTCAGCTCCCTCCTGACTTCAAATGGGCAATTCGGGTCCCTTCTGGAGGGTCTGAATCCAAATGGGTCGGGTCTGAAACTGATGCAAATGGGTGATTTTGGGGAGAATTTGGATGCTGCAGGTAATATGATGAATTCGGATCCGGGTCCGGGTTTGGAGGGCCAGAGCCATGGGAATCCAGAGAGCTATATGGGGTTGCAAAATGGTGATTCTTCAACGTCAAGCTGTTGGAATGGGGCCAATGGGTGGCCTGACCTTGCCATTTATACACCAGGTTCAAGTTTTCAGTAG

Protein Analysis

340

Amino Acids

37.68

Weight (kDa)

9.4

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 132 - 188 1.3e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51700 AT3G21270
fragaria_vesca FvH4_6g32990
malus_domestica MD09G1195800.v1.1 MD17G1176100.v1.1
prunus_persica Prupe.3G052300_v2.0.a1
pyrus_communis pycom17g18370
rosa_chinensis RchiOBHm_Chr2g0142541
rosa_laevigata RLG00000019965
rosa_multiflora Rmu_co8311091.1_g000001
rosa_roxburghii Rroxscaffold_1G00029420 Rroxscaffold_2G00102540
rosa_rugosa Rorug02G0373800
rosa_samantha Rh2AG425100 Rh2BG435000 Rh2CG411700 Rh2DG445000
rosa_wichuraiana Rw2G034870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 361, 839
AccB7I CCANNNNNTGG 1 cut(s) 975
AccII CGCG 3 cut(s) 412, 472, 705
AciI CCGC 9 cut(s) 410, 470, 496, 513, 540, 543, 656, 703, 705
AclI AACGTT 1 cut(s) 589
AclWI GGATC 6 cut(s) 94, 129, 647, 660, 863, 876
AcsI RAATTY 6 cut(s) 111, 172, 240, 254, 835, 862
AcuI CTGAAG 2 cut(s) 612, 715
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 6 cut(s) 310, 539, 673, 775, 878, 975
AgsI TTSAA 5 cut(s) 11, 246, 750, 945, 1008
AjnI CCWGG 1 cut(s) 1000
AluBI AGCT 8 cut(s) 400, 581, 624, 641, 729, 735, 915, 954
AluI AGCT 8 cut(s) 400, 581, 624, 641, 729, 735, 915, 954
Alw21I GWGCWC 1 cut(s) 626
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 6 cut(s) 94, 129, 647, 660, 863, 876
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 4 cut(s) 675, 889, 966, 977
ApeKI GCWGC 8 cut(s) 397, 414, 466, 632, 635, 638, 658, 845
ApoI RAATTY 6 cut(s) 111, 172, 240, 254, 835, 862
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AspLEI GCGC 3 cut(s) 414, 474, 518
AspS9I GGNCC 7 cut(s) 504, 669, 675, 765, 875, 889, 966
AsuC2I CCSGG 5 cut(s) 650, 673, 674, 873, 879
AsuHPI GGTGA 3 cut(s) 275, 833, 947
AsuII TTCGAA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 4 cut(s) 504, 669, 765, 875
BamHI GGATCC 2 cut(s) 652, 868
BanII GRGCYC 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 626
BbvI GCAGC 8 cut(s) 401, 409, 478, 644, 647, 650, 670, 832
BccI CCATC 1 cut(s) 706
BceAI ACGGC 1 cut(s) 697
BciT130I CCWGG 1 cut(s) 1002
BcnI CCSGG 5 cut(s) 650, 673, 674, 873, 879
BcoDI GTCTC 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 2 cut(s) 361, 839
Bme1390I CCNGG 6 cut(s) 650, 673, 674, 873, 879, 1002
Bme18I GGWCC 4 cut(s) 504, 669, 765, 875
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 7 cut(s) 504, 669, 675, 765, 875, 889, 966
BmiI GGNNCC 8 cut(s) 351, 654, 671, 766, 767, 870, 876, 967
BmrFI CCNGG 6 cut(s) 650, 673, 674, 873, 879, 1002
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 2 cut(s) 801, 832
BmuI ACTGGG 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 794
Bpu14I TTCGAA 1 cut(s) 584
BpuMI CCSGG 5 cut(s) 650, 673, 674, 873, 879
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 672, 703, 898
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 6 cut(s) 310, 539, 673, 775, 878, 975
Bse1I ACTGG 2 cut(s) 379, 506
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 3 cut(s) 672, 703, 898
BseGI GGATG 1 cut(s) 847
BseLI CCNNNNNNNGG 6 cut(s) 310, 539, 673, 775, 878, 975
BseMII CTCAG 2 cut(s) 656, 735
BseNI ACTGG 2 cut(s) 379, 506
BseXI GCAGC 8 cut(s) 401, 409, 478, 644, 647, 650, 670, 832
Bsh1236I CGCG 3 cut(s) 412, 472, 705
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 4 cut(s) 677, 891, 968, 979
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 626
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 5 cut(s) 650, 667, 673, 872, 878
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 6 cut(s) 310, 539, 673, 775, 878, 975
BsmAI GTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 751
BsnI GGCC 4 cut(s) 677, 891, 968, 979
Bso31I GGTCTC 1 cut(s) 339
BsoBI CYCGRG 1 cut(s) 672
Bsp119I TTCGAA 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 626
Bsp143I GATC 6 cut(s) 99, 134, 280, 652, 694, 868
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 9 cut(s) 410, 470, 496, 513, 540, 543, 656, 703, 705
BspANI GGCC 4 cut(s) 677, 891, 968, 979
BspCNI CTCAG 2 cut(s) 655, 734
BspFNI CGCG 3 cut(s) 412, 472, 705
BspLI GGNNCC 8 cut(s) 351, 654, 671, 766, 767, 870, 876, 967
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 2 cut(s) 361, 839
BspPI GGATC 6 cut(s) 94, 129, 647, 660, 863, 876
BspQI GCTCTTC 1 cut(s) 631
BspT104I TTCGAA 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 339
BsrI ACTGG 2 cut(s) 379, 506
BssECI CCNNGG 3 cut(s) 672, 703, 898
BssMI GATC 6 cut(s) 99, 134, 280, 652, 694, 868
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 1 cut(s) 618
Bst6I CTCTTC 2 cut(s) 209, 631
BstAPI GCANNNNNTGC 1 cut(s) 489
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 2 cut(s) 642, 721
BstDSI CCRYGG 2 cut(s) 703, 898
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 1 cut(s) 847
BstFNI CGCG 3 cut(s) 412, 472, 705
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 3 cut(s) 414, 474, 518
BstKTI GATC 6 cut(s) 102, 137, 283, 655, 697, 871
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 6 cut(s) 99, 134, 280, 652, 694, 868
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 5 cut(s) 397, 480, 489, 546, 638
BstNI CCWGG 1 cut(s) 1002
BstSCI CCNGG 6 cut(s) 648, 671, 672, 871, 877, 1000
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 3 cut(s) 412, 472, 705
BstV1I GCAGC 8 cut(s) 401, 409, 478, 644, 647, 650, 670, 832
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 3 cut(s) 99, 652, 868
BstXI CCANNNNNNTGG 1 cut(s) 899
BstYI RGATCY 3 cut(s) 99, 652, 868
BsuRI GGCC 4 cut(s) 677, 891, 968, 979
BtgI CCRYGG 2 cut(s) 703, 898
BtsCI GGATG 1 cut(s) 847
BtsIMutI CAGTG 1 cut(s) 614
BveI ACCTGC 2 cut(s) 361, 839
Cac8I GCNNGC 1 cut(s) 494
CfoI GCGC 3 cut(s) 414, 474, 518
Cfr13I GGNCC 7 cut(s) 504, 669, 675, 765, 875, 889, 966
Cfr42I CCGCGG 1 cut(s) 706
Cfr9I CCCGGG 1 cut(s) 672
CsiI ACCWGGT 1 cut(s) 1000
Csp6I GTAC 1 cut(s) 499
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 2 cut(s) 370, 899
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 2 cut(s) 642, 721
DpnI GATC 6 cut(s) 101, 136, 282, 654, 696, 870
DpnII GATC 6 cut(s) 99, 134, 280, 652, 694, 868
Eam1104I CTCTTC 2 cut(s) 209, 631
EarI CTCTTC 2 cut(s) 209, 631
EciI GGCGGA 1 cut(s) 558
Ecl136II GAGCTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 626
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 4 cut(s) 504, 669, 765, 875
Eco53kI GAGCTC 1 cut(s) 624
Eco57I CTGAAG 2 cut(s) 612, 715
Eco88I CYCGRG 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 624
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 765
EcoRI GAATTC 2 cut(s) 254, 862
EcoRII CCWGG 1 cut(s) 1000
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 2 cut(s) 373, 902
FaiI YATR 8 cut(s) 194, 297, 371, 857, 900, 918, 920, 996
FaqI GGGAC 1 cut(s) 751
FatI CATG 2 cut(s) 369, 898
FokI GGATG 1 cut(s) 854
FriOI GRGCYC 1 cut(s) 626
GlaI GCGC 3 cut(s) 413, 473, 517
GsuI CTGGAG 1 cut(s) 794
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 4 cut(s) 677, 891, 968, 979
HapII CCGG 5 cut(s) 650, 667, 673, 872, 878
HhaI GCGC 3 cut(s) 414, 474, 518
Hin1II CATG 2 cut(s) 373, 902
Hin6I GCGC 3 cut(s) 412, 472, 516
HinP1I GCGC 3 cut(s) 412, 472, 516
HinfI GANTC 5 cut(s) 146, 419, 784, 904, 938
HpaII CCGG 5 cut(s) 650, 667, 673, 872, 878
HphI GGTGA 3 cut(s) 275, 833, 947
Hpy188I TCNGA 8 cut(s) 43, 99, 253, 265, 645, 783, 804, 868
Hpy188III TCNNGA 6 cut(s) 303, 386, 698, 742, 773, 908
Hpy99I CGWCG 3 cut(s) 539, 574, 577
HpyAV CCTTC 1 cut(s) 779
HpyCH4III ACNGT 1 cut(s) 618
HpyCH4IV ACGT 2 cut(s) 589, 947
HpyCH4V TGCA 8 cut(s) 51, 346, 356, 483, 492, 814, 848, 928
HpyF10VI GCNNNNNNNGC 5 cut(s) 397, 480, 489, 546, 638
HpyF3I CTNAG 2 cut(s) 642, 721
HpySE526I ACGT 2 cut(s) 589, 947
Hsp92II CATG 2 cut(s) 373, 902
HspAI GCGC 3 cut(s) 412, 472, 516
KflI GGGWCCC 1 cut(s) 765
KspI CCGCGG 1 cut(s) 706
Kzo9I GATC 6 cut(s) 99, 134, 280, 652, 694, 868
LguI GCTCTTC 1 cut(s) 631
LmnI GCTCC 2 cut(s) 726, 740
Lsp1109I GCAGC 8 cut(s) 401, 409, 478, 644, 647, 650, 670, 832
LweI GCATC 2 cut(s) 801, 832
MabI ACCWGGT 1 cut(s) 1000
MaeII ACGT 2 cut(s) 589, 947
MaeIII GTNAC 1 cut(s) 612
MalI GATC 6 cut(s) 101, 136, 282, 654, 696, 870
MboI GATC 6 cut(s) 99, 134, 280, 652, 694, 868
MboII GAAGA 6 cut(s) 25, 196, 283, 574, 618, 933
MflI RGATCY 3 cut(s) 99, 652, 868
MhlI GDGCHC 1 cut(s) 626
MluCI AATT 8 cut(s) 111, 139, 172, 240, 254, 758, 835, 862
MlyI GAGTC 2 cut(s) 140, 428
MmeI TCCRAC 3 cut(s) 388, 450, 938
MnlI CCTC 9 cut(s) 177, 212, 433, 470, 604, 616, 749, 769, 880
MseI TTAA 2 cut(s) 84, 237
MspA1I CMGCKG 2 cut(s) 400, 705
MspI CCGG 5 cut(s) 650, 667, 673, 872, 878
MspR9I CCNGG 6 cut(s) 650, 673, 674, 873, 879, 1002
MvaI CCWGG 1 cut(s) 1002
MvnI CGCG 3 cut(s) 412, 472, 705
MwoI GCNNNNNNNGC 5 cut(s) 397, 480, 489, 546, 638
NciI CCSGG 5 cut(s) 650, 673, 674, 873, 879
NcoI CCATGG 1 cut(s) 898
NdeII GATC 6 cut(s) 99, 134, 280, 652, 694, 868
NlaIII CATG 2 cut(s) 373, 902
NlaIV GGNNCC 8 cut(s) 351, 654, 671, 766, 767, 870, 876, 967
NmuCI GTSAC 1 cut(s) 612
NspV TTCGAA 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 631
PcsI WCGNNNNNNNCGW 3 cut(s) 566, 581, 617
PfeI GAWTC 3 cut(s) 784, 904, 938
PflMI CCANNNNNTGG 1 cut(s) 975
PleI GAGTC 2 cut(s) 140, 427
PpsI GAGTC 2 cut(s) 140, 427
PpuMI RGGWCCY 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 626
Psp1406I AACGTT 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 765
Psp6I CCWGG 1 cut(s) 1000
PspGI CCWGG 1 cut(s) 1000
PspN4I GGNNCC 8 cut(s) 351, 654, 671, 766, 767, 870, 876, 967
PspPI GGNCC 7 cut(s) 504, 669, 675, 765, 875, 889, 966
PspPPI RGGWCCY 1 cut(s) 765
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 3 cut(s) 99, 652, 868
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SacI GAGCTC 1 cut(s) 626
SacII CCGCGG 1 cut(s) 706
SapI GCTCTTC 1 cut(s) 631
SaqAI TTAA 2 cut(s) 84, 237
Sau3AI GATC 6 cut(s) 99, 134, 280, 652, 694, 868
Sau96I GGNCC 7 cut(s) 504, 669, 675, 765, 875, 889, 966
SchI GAGTC 2 cut(s) 140, 428
ScrFI CCNGG 6 cut(s) 650, 673, 674, 873, 879, 1002
SduI GDGCHC 1 cut(s) 626
SexAI ACCWGGT 1 cut(s) 1000
SfaNI GCATC 2 cut(s) 801, 832
SfcI CTRYAG 1 cut(s) 846
Sfr303I CCGCGG 1 cut(s) 706
SfuI TTCGAA 1 cut(s) 584
SgrBI CCGCGG 1 cut(s) 706
SinI GGWCC 4 cut(s) 504, 669, 765, 875
SmaI CCCGGG 1 cut(s) 674
Sse9I AATT 8 cut(s) 111, 139, 172, 240, 254, 758, 835, 862
SsiI CCGC 9 cut(s) 410, 470, 496, 513, 540, 543, 656, 703, 705
SstI GAGCTC 1 cut(s) 626
StyD4I CCNGG 6 cut(s) 648, 671, 672, 871, 877, 1000
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 1 cut(s) 618
TaiI ACGT 2 cut(s) 592, 950
TaqI TCGA 4 cut(s) 584, 599, 620, 693
TasI AATT 8 cut(s) 111, 139, 172, 240, 254, 758, 835, 862
TauI GCSGC 3 cut(s) 472, 516, 543
TfiI GAWTC 3 cut(s) 784, 904, 938
Tru1I TTAA 2 cut(s) 84, 237
Tru9I TTAA 2 cut(s) 84, 237
TscAI CASTG 1 cut(s) 621
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 8 cut(s) 397, 414, 466, 632, 635, 638, 658, 845
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 17, 210, 386, 875
TspMI CCCGGG 1 cut(s) 672
TspRI CASTG 1 cut(s) 621
Van91I CCANNNNNTGG 1 cut(s) 975
VpaK11BI GGWCC 4 cut(s) 504, 669, 765, 875
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 6 cut(s) 111, 172, 240, 254, 835, 862
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.