Rh2AG425100

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013234
Physical Location & Seq
Reverse (-)
63220398 .. 63223173
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG425100.1

Sequence Viewer

Length: 690 bp
ATGCAAGACCCAACAACGTTCCAATCCATGAAAGCCCAATTCCCAGAGCAAGAGCAGCTGAAATGTCCACGCTGTGATTCCTCAAACACCAAGTTCTGCTACTACAACAACTACAATCTGTCGCAGCCTCGCCACTTCTGCAAGAACTGCAAGAGGTACTGGACCAAAGGTGGTTCCCTCAGAAACATTCCAATAGGCGGTGGAAGCAGAAAGAACACCAAGAGATCTTCTTCGGGTTCGAAACGTTCTTCATCGGCTACTAATTCCTCATCATCAACCCTGTCAAGCTCAGCAGCTGCAGTTGCAGCAGCAGCAGCAGCAGCTCAGAACCAGGATCCGCAGCCGGACCGGACTCGGCTCTACGGCCCGAAAATGGATCCAGATAATCCGATGCTGGATATTTCTGGGAGCTTCAGCTCACTGTTAACATCTAATGATCAGTTTGGGTCTCTTATGGAGGGTCTGAATCCAAATGGGTCGGGGTTGAAACTGATGCAAATGGGTGAGTTTGGGGAGAATGTGGTGGATTCATCAGGACATGGGTTGAATTCGGATCCGGGTTTGGAGGTCCAGAGCAGTGGGAACCCAGGGAGCTATATGGGTTTGCAAAGTGGGGATTCGAGCTGTTGGAATGGGAGCAATGGGTGGCCTGACCTTGCCATTTACACACCAGGTTCAAGTTTTCAGTAG

Protein Analysis

229

Amino Acids

24.47

Weight (kDa)

8.19

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 18 - 74 8.5e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51700 AT3G21270
fragaria_vesca FvH4_6g32990
malus_domestica MD09G1195800.v1.1 MD17G1176100.v1.1
prunus_persica Prupe.3G052300_v2.0.a1
pyrus_communis pycom17g18370
rosa_chinensis RchiOBHm_Chr2g0142541
rosa_laevigata RLG00000019965
rosa_multiflora Rmu_co8311091.1_g000001
rosa_roxburghii Rroxscaffold_1G00029420 Rroxscaffold_2G00102540
rosa_rugosa Rorug02G0373800
rosa_samantha Rh2AG425100 Rh2BG435000 Rh2CG411700 Rh2DG445000
rosa_wichuraiana Rw2G034870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 198, 338
AclI AACGTT 2 cut(s) 17, 244
AclWI GGATC 6 cut(s) 329, 342, 371, 384, 548, 561
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 397
AdeI CACNNNGTG 1 cut(s) 74
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 197, 373
AgsI TTSAA 3 cut(s) 487, 547, 678
AjnI CCWGG 3 cut(s) 330, 586, 670
AluBI AGCT 8 cut(s) 58, 288, 296, 323, 411, 417, 594, 624
AluI AGCT 8 cut(s) 58, 288, 296, 323, 411, 417, 594, 624
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 6 cut(s) 329, 342, 371, 384, 548, 561
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 2 cut(s) 364, 647
ApoI RAATTY 1 cut(s) 547
AspS9I GGNCC 4 cut(s) 162, 346, 365, 568
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 515
AsuII TTCGAA 1 cut(s) 239
AvaII GGWCC 3 cut(s) 162, 346, 568
BamHI GGATCC 3 cut(s) 334, 376, 553
BceAI ACGGC 1 cut(s) 379
BciT130I CCWGG 3 cut(s) 332, 588, 672
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 224
BlpI GCTNAGC 1 cut(s) 289
Bme1390I CCNGG 4 cut(s) 332, 558, 588, 672
Bme18I GGWCC 3 cut(s) 162, 346, 568
BmgT120I GGNCC 4 cut(s) 162, 346, 365, 568
BmiI GGNNCC 5 cut(s) 175, 336, 378, 555, 584
BmrFI CCNGG 4 cut(s) 332, 558, 588, 672
BmsI GCATC 2 cut(s) 381, 483
Bpu1102I GCTNAGC 1 cut(s) 289
Bpu14I TTCGAA 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 586, 587
BsaWI WCCGGW 1 cut(s) 348
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 373
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 646
BseBI CCWGG 3 cut(s) 332, 588, 672
BseDI CCNNGG 2 cut(s) 586, 587
BseLI CCNNNNNNNGG 2 cut(s) 197, 373
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 3 cut(s) 193, 303, 338
BseNI ACTGG 1 cut(s) 164
BshFI GGCC 2 cut(s) 366, 649
BsiSI CCGG 3 cut(s) 344, 349, 557
BslI CCNNNNNNNGG 2 cut(s) 197, 373
BsmAI GTCTC 1 cut(s) 453
BsnI GGCC 2 cut(s) 366, 649
Bso31I GGTCTC 1 cut(s) 453
Bsp119I TTCGAA 1 cut(s) 239
Bsp143I GATC 5 cut(s) 224, 334, 376, 436, 553
Bsp1720I GCTNAGC 1 cut(s) 289
BspACI CCGC 2 cut(s) 198, 338
BspANI GGCC 2 cut(s) 366, 649
BspCNI CTCAG 3 cut(s) 192, 302, 337
BspLI GGNNCC 5 cut(s) 175, 336, 378, 555, 584
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 6 cut(s) 329, 342, 371, 384, 548, 561
BspT104I TTCGAA 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 646
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 586, 587
BssMI GATC 5 cut(s) 224, 334, 376, 436, 553
Bst2UI CCWGG 3 cut(s) 332, 588, 672
Bst4CI ACNGT 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 239
BstDEI CTNAG 3 cut(s) 179, 289, 324
BstKTI GATC 5 cut(s) 227, 337, 379, 439, 556
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 5 cut(s) 224, 334, 376, 436, 553
BstNI CCWGG 3 cut(s) 332, 588, 672
BstSCI CCNGG 4 cut(s) 330, 556, 586, 670
BstSFI CTRYAG 1 cut(s) 297
BstX2I RGATCY 4 cut(s) 224, 334, 376, 553
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 4 cut(s) 224, 334, 376, 553
BsuRI GGCC 2 cut(s) 366, 649
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 2 cut(s) 419, 583
CaiI CAGNNNCTG 1 cut(s) 296
Cfr13I GGNCC 4 cut(s) 162, 346, 365, 568
CpoI CGGWCCG 1 cut(s) 346
CsiI ACCWGGT 1 cut(s) 670
Csp6I GTAC 1 cut(s) 157
CspI CGGWCCG 1 cut(s) 346
CviAII CATG 2 cut(s) 28, 539
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 179, 289, 324
DpnI GATC 5 cut(s) 226, 336, 378, 438, 555
DpnII GATC 5 cut(s) 224, 334, 376, 436, 553
DraIII CACNNNGTG 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 453
Eco47I GGWCC 3 cut(s) 162, 346, 568
Eco57I CTGAAG 1 cut(s) 397
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 3 cut(s) 330, 586, 670
FaeI CATG 2 cut(s) 31, 542
FaiI YATR 5 cut(s) 29, 455, 540, 597, 599
FatI CATG 2 cut(s) 27, 538
FbaI TGATCA 1 cut(s) 436
HaeIII GGCC 2 cut(s) 366, 649
HapII CCGG 3 cut(s) 344, 349, 557
Hin1II CATG 2 cut(s) 31, 542
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 5 cut(s) 77, 352, 466, 527, 617
HpaI GTTAAC 1 cut(s) 426
HpaII CCGG 3 cut(s) 344, 349, 557
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 2 cut(s) 68, 426
Hpy188I TCNGA 5 cut(s) 182, 327, 390, 465, 553
Hpy188III TCNNGA 3 cut(s) 380, 534, 571
Hpy8I GTNNAC 2 cut(s) 68, 426
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 2 cut(s) 17, 244
HpyCH4V TGCA 7 cut(s) 4, 141, 150, 299, 305, 496, 607
HpyF3I CTNAG 3 cut(s) 179, 289, 324
HpySE526I ACGT 2 cut(s) 17, 244
Hsp92II CATG 2 cut(s) 31, 542
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 426
Kzo9I GATC 5 cut(s) 224, 334, 376, 436, 553
LmnI GCTCC 3 cut(s) 408, 591, 636
LweI GCATC 2 cut(s) 381, 483
MabI ACCWGGT 1 cut(s) 670
MaeII ACGT 2 cut(s) 17, 244
MalI GATC 5 cut(s) 226, 336, 378, 438, 555
MboI GATC 5 cut(s) 224, 334, 376, 436, 553
MboII GAAGA 3 cut(s) 219, 222, 240
MflI RGATCY 4 cut(s) 224, 334, 376, 553
MluCI AATT 3 cut(s) 38, 262, 547
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 608
MnlI CCTC 7 cut(s) 91, 138, 147, 188, 277, 451, 559
MseI TTAA 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 58, 296
MspI CCGG 3 cut(s) 344, 349, 557
MspR9I CCNGG 4 cut(s) 332, 558, 588, 672
MvaI CCWGG 3 cut(s) 332, 588, 672
NciI CCSGG 1 cut(s) 558
NdeII GATC 5 cut(s) 224, 334, 376, 436, 553
NlaIII CATG 2 cut(s) 31, 542
NlaIV GGNNCC 5 cut(s) 175, 336, 378, 555, 584
NmeAIII GCCGAG 1 cut(s) 334
NspV TTCGAA 1 cut(s) 239
PasI CCCWGGG 1 cut(s) 587
PfeI GAWTC 4 cut(s) 77, 466, 527, 617
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Psp1406I AACGTT 2 cut(s) 17, 244
Psp6I CCWGG 3 cut(s) 330, 586, 670
PspGI CCWGG 3 cut(s) 330, 586, 670
PspN4I GGNNCC 5 cut(s) 175, 336, 378, 555, 584
PspPI GGNCC 4 cut(s) 162, 346, 365, 568
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 4 cut(s) 224, 334, 376, 553
PvuII CAGCTG 2 cut(s) 58, 296
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
Rsr2I CGGWCCG 1 cut(s) 346
RsrII CGGWCCG 1 cut(s) 346
SaqAI TTAA 1 cut(s) 425
Sau3AI GATC 5 cut(s) 224, 334, 376, 436, 553
Sau96I GGNCC 4 cut(s) 162, 346, 365, 568
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 4 cut(s) 332, 558, 588, 672
SexAI ACCWGGT 1 cut(s) 670
SfaNI GCATC 2 cut(s) 381, 483
SfcI CTRYAG 1 cut(s) 297
SfuI TTCGAA 1 cut(s) 239
SinI GGWCC 3 cut(s) 162, 346, 568
Sse9I AATT 3 cut(s) 38, 262, 547
SsiI CCGC 2 cut(s) 198, 338
StyD4I CCNGG 4 cut(s) 330, 556, 586, 670
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 2 cut(s) 20, 247
TaqI TCGA 2 cut(s) 239, 620
TasI AATT 3 cut(s) 38, 262, 547
TfiI GAWTC 4 cut(s) 77, 466, 527, 617
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 2 cut(s) 426, 583
TspDTI ATGAA 3 cut(s) 44, 240, 519
TspRI CASTG 2 cut(s) 426, 583
VpaK11BI GGWCC 3 cut(s) 162, 346, 568
XapI RAATTY 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.