pycom17g18790

40S ribosomal protein S30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003860
Physical Location & Seq
Reverse (-)
16243459 .. 16243981
523 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 189 bp
ATGGGCAAGGTTCACGGTTCGCTCGCACGTGCCGGGAAGGTTAGAGGTCAGACTCCGAAGGTGGCCAAGCAAGACAAGAAGAAAAAGCCCAGAGGCCGCGCGCACAAGAGGCTGCAGTACAACAGGAGATTCGTCACGGCGGTTGTCGGGTTTGGGAAGAAGAGGGGGCCGAACTCTTCCGAGAAGTAA

Protein Analysis

63

Amino Acids

6.91

Weight (kDa)

12.0

Isoelectric Point (pI)

28.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S30 PF04758 3 - 59 1.1e-30 Ribosomal protein S30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 99, 101
AciI CCGC 2 cut(s) 97, 140
AcoI YGGCCR 1 cut(s) 63
AcvI CACGTG 1 cut(s) 29
AfaI GTAC 1 cut(s) 119
AoxI GGCC 3 cut(s) 63, 94, 167
ApeKI GCWGC 1 cut(s) 112
AspLEI GCGC 2 cut(s) 101, 103
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 34
BalI TGGCCA 1 cut(s) 65
BbrPI CACGTG 1 cut(s) 29
BbvI GCAGC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 34
BfmI CTRYAG 1 cut(s) 113
BisI GCNGC 2 cut(s) 97, 113
BlsI GCNGC 2 cut(s) 98, 114
Bme1390I CCNGG 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 34
BsaAI YACGTR 1 cut(s) 29
BsePI GCGCGC 1 cut(s) 99
BseXI GCAGC 1 cut(s) 99
Bsh1236I CGCG 2 cut(s) 99, 101
BshFI GGCC 3 cut(s) 65, 96, 169
BsiSI CCGG 1 cut(s) 33
BsnI GGCC 3 cut(s) 65, 96, 169
BspACI CCGC 2 cut(s) 97, 140
BspANI GGCC 3 cut(s) 65, 96, 169
BspFNI CGCG 2 cut(s) 99, 101
BspLI GGNNCC 1 cut(s) 168
BspMAI CTGCAG 1 cut(s) 117
BssHII GCGCGC 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 155, 181
BstBAI YACGTR 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 24, 101
BstFNI CGCG 2 cut(s) 99, 101
BstHHI GCGC 2 cut(s) 101, 103
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 113
BstUI CGCG 2 cut(s) 99, 101
BstV1I GCAGC 1 cut(s) 99
BsuRI GGCC 3 cut(s) 65, 96, 169
Cac8I GCNNGC 2 cut(s) 24, 101
CfoI GCGC 2 cut(s) 101, 103
Cfr13I GGNCC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 118
CviJI RGCY 5 cut(s) 65, 88, 96, 112, 169
CviKI_1 RGCY 5 cut(s) 65, 88, 96, 112, 169
CviQI GTAC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 155, 181
EarI CTCTTC 2 cut(s) 155, 181
Eco72I CACGTG 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 97, 113
Fsp4HI GCNGC 2 cut(s) 97, 113
GlaI GCGC 2 cut(s) 100, 102
GluI GCNGC 2 cut(s) 97, 113
HaeIII GGCC 3 cut(s) 65, 96, 169
HapII CCGG 1 cut(s) 33
HhaI GCGC 2 cut(s) 101, 103
Hin6I GCGC 2 cut(s) 99, 101
HinP1I GCGC 2 cut(s) 99, 101
HinfI GANTC 2 cut(s) 52, 129
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 51, 57, 181
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 31, 52
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpySE526I ACGT 1 cut(s) 28
HspAI GCGC 2 cut(s) 99, 101
LpnPI CCDG 3 cut(s) 46, 103, 109
Lsp1109I GCAGC 1 cut(s) 99
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 133
MboII GAAGA 4 cut(s) 91, 168, 169, 172
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 4 cut(s) 38, 86, 102, 156
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 34
MvnI CGCG 2 cut(s) 99, 101
MwoI GCNNNNNNNGC 1 cut(s) 109
NciI CCSGG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 133
PauI GCGCGC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 98, 114
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 29
PmlI CACGTG 1 cut(s) 29
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 29
PspCI CACGTG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 167
PstI CTGCAG 1 cut(s) 117
PteI GCGCGC 1 cut(s) 99
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SatI GCNGC 2 cut(s) 97, 113
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 34
SetI ASST 5 cut(s) 12, 31, 42, 49, 63
SfcI CTRYAG 1 cut(s) 113
SsiI CCGC 2 cut(s) 97, 140
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 31
TatI WGTACW 1 cut(s) 117
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 129
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.