pycom17g20830

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007344
Physical Location & Seq
Forward (+)
19265649 .. 19268769
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 843 bp
ATGTCAAGACCCATATCTTTATCTCCTATACATAAAAGTCCGGTTATAGAAGTTGAATCGTCTTTAAGTACCTCAGAAAATGTACCAGCGAGATCCACCTTAACTAGAGCAGGGAGTCCTGAACAAGACACAGCTGATTCAAGAACTTCTGGAACTATAAAGGGTGGGGGAAGGTCCTCAGACCTAGGGCTTCTCTTTGCTAATGCAGATGCAAGTCATCCAGGAAGAGTTGGAGGAAACTACCAGACTGACACATCAAGCAACATCATTGGCGATATAGATCCCATGGATATGTCAGAGAGTATGCAAGAAGCAATGCACCGTTCTAACTTGGATATGAGTGGTGCCGAGTATGTCAGGAAAGAAGTTCCTCTTCATGTTTTACCGACCTCGTCTCTTGTTAAGCTCGATTCACCTTTAATGTCATTCACAGAATTGCAACGAGTACTGTATGAAGAGGAGCAAGCGGCTTATAACCAAGCAATCTTGCAAAATATGAGGGATGGGAAGGTGCATCCACTTACTTTCATACACCATACATCAATGTACCAGTCTTCCATGTGCAAATTAATCGAATATTGTTTGAGTCCTGCCATAAGTGCACTGCAATGTCGGTTAAGAGAGAATGAAACTCGGTTAGCAGTGCTCACCGATGAAGTGAAGAATTTGGAAACCGAGATGCATAGAACAAGTGAGTCAAACTCAGGATCTCCTCGTCCAGTTTCATCGCCTGTACTCCGTGGAAGTGCTCCGTTGGGGCACAGGGACTTGTTTAATCCAACTGAATCTGTCAGTGCAAGGAGTCTGGCTGGTTCTGGCAGTCGGAGCCGAAAAGGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

281

Amino Acids

30.43

Weight (kDa)

6.0

Isoelectric Point (pI)

76.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCC36_C PF18110 140 - 223 7.3e-23 BRCC36 C-terminal helical domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 474
AccB1I GGYRCC 1 cut(s) 344
AciI CCGC 1 cut(s) 467
AclWI GGATC 3 cut(s) 87, 275, 715
AcsI RAATTY 1 cut(s) 664
AfaI GTAC 5 cut(s) 70, 84, 447, 548, 735
AgsI TTSAA 2 cut(s) 56, 141
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 2 cut(s) 134, 406
AluI AGCT 2 cut(s) 134, 406
Alw21I GWGCWC 3 cut(s) 604, 648, 751
Alw26I GTCTC 1 cut(s) 399
Alw44I GTGCAC 1 cut(s) 600
AlwI GGATC 3 cut(s) 87, 275, 715
ApaLI GTGCAC 1 cut(s) 600
ApoI RAATTY 1 cut(s) 664
AseI ATTAAT 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 835
AspA2I CCTAGG 1 cut(s) 184
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 2 cut(s) 405, 640
AvaII GGWCC 1 cut(s) 174
AvrII CCTAGG 1 cut(s) 184
BaeGI GKGCMC 2 cut(s) 604, 762
BanI GGYRCC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 3 cut(s) 604, 648, 751
BccI CCATC 1 cut(s) 497
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 2 cut(s) 105, 185
BisI GCNGC 1 cut(s) 468
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 1 cut(s) 469
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 346, 827
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 3 cut(s) 199, 523, 669
BpiI GAAGAC 1 cut(s) 546
BplI GAGNNNNNCTC 2 cut(s) 686, 718
BsaBI GATNNNNATC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 184, 285, 739
BsaWI WCCGGW 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 550, 719
Bse3DI GCAATG 2 cut(s) 321, 614
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 3 cut(s) 184, 285, 739
BseGI GGATG 3 cut(s) 217, 508, 514
BseJI GATNNNNATC 1 cut(s) 279
BseMI GCAATG 2 cut(s) 321, 614
BseMII CTCAG 3 cut(s) 87, 192, 717
BseNI ACTGG 2 cut(s) 550, 719
BseRI GAGGAG 2 cut(s) 473, 702
BseSI GKGCMC 2 cut(s) 604, 762
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 3 cut(s) 604, 648, 751
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 1 cut(s) 779
BsmAI GTCTC 1 cut(s) 399
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 1 cut(s) 779
Bsp1286I GDGCHC 4 cut(s) 604, 648, 751, 762
Bsp143I GATC 3 cut(s) 92, 280, 707
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 1 cut(s) 467
BspCNI CTCAG 3 cut(s) 86, 191, 716
BspLI GGNNCC 2 cut(s) 346, 827
BspPI GGATC 3 cut(s) 87, 275, 715
BspT107I GGYRCC 1 cut(s) 344
BsrDI GCAATG 2 cut(s) 321, 614
BsrI ACTGG 2 cut(s) 550, 719
BssECI CCNNGG 3 cut(s) 184, 285, 739
BssMI GATC 3 cut(s) 92, 280, 707
BssT1I CCWWGG 2 cut(s) 184, 285
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 2 cut(s) 323, 450
Bst6I CTCTTC 3 cut(s) 220, 378, 450
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 3 cut(s) 73, 178, 703
BstDSI CCRYGG 2 cut(s) 285, 739
BstF5I GGATG 3 cut(s) 217, 508, 514
BstKTI GATC 3 cut(s) 95, 283, 710
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 3 cut(s) 92, 280, 707
BstMWI GCNNNNNNNGC 2 cut(s) 599, 825
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 220
BstSLI GKGCMC 2 cut(s) 604, 762
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 3 cut(s) 92, 280, 707
BstYI RGATCY 3 cut(s) 92, 280, 707
BtgI CCRYGG 2 cut(s) 285, 739
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 3 cut(s) 217, 508, 514
BtsI GCAGTG 2 cut(s) 602, 648
BtsIMutI CAGTG 3 cut(s) 602, 648, 799
Cac8I GCNNGC 1 cut(s) 465
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 5 cut(s) 69, 83, 446, 547, 734
CviAII CATG 3 cut(s) 286, 377, 559
CviJI RGCY 6 cut(s) 134, 190, 406, 470, 809, 828
CviKI_1 RGCY 6 cut(s) 134, 190, 406, 470, 809, 828
CviQI GTAC 5 cut(s) 69, 83, 446, 547, 734
DdeI CTNAG 3 cut(s) 73, 178, 703
DpnI GATC 3 cut(s) 94, 282, 709
DpnII GATC 3 cut(s) 92, 280, 707
Eam1104I CTCTTC 3 cut(s) 220, 378, 450
EarI CTCTTC 3 cut(s) 220, 378, 450
Eco130I CCWWGG 2 cut(s) 184, 285
Eco47I GGWCC 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 184, 285
EcoT22I ATGCAT 1 cut(s) 684
ErhI CCWWGG 2 cut(s) 184, 285
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 3 cut(s) 289, 380, 562
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 779
FatI CATG 3 cut(s) 285, 376, 558
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 3 cut(s) 204, 501, 515
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 2 cut(s) 105, 185
GluI GCNGC 1 cut(s) 468
HapII CCGG 1 cut(s) 41
Hin1II CATG 3 cut(s) 289, 380, 562
HinfI GANTC 8 cut(s) 56, 115, 137, 410, 586, 695, 785, 802
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 405, 640
Hpy166II GTNNAC 1 cut(s) 602
Hpy188I TCNGA 4 cut(s) 76, 181, 298, 825
Hpy188III TCNNGA 6 cut(s) 6, 119, 141, 150, 358, 705
Hpy8I GTNNAC 1 cut(s) 602
HpyAV CCTTC 2 cut(s) 165, 502
HpyCH4III ACNGT 2 cut(s) 323, 450
HpyF10VI GCNNNNNNNGC 2 cut(s) 599, 825
HpyF3I CTNAG 3 cut(s) 73, 178, 703
Hsp92II CATG 3 cut(s) 289, 380, 562
Kzo9I GATC 3 cut(s) 92, 280, 707
LmnI GCTCC 3 cut(s) 460, 754, 825
LweI GCATC 3 cut(s) 199, 523, 669
MaeI CTAG 2 cut(s) 105, 185
MalI GATC 3 cut(s) 94, 282, 709
MboI GATC 3 cut(s) 92, 280, 707
MboII GAAGA 5 cut(s) 237, 365, 467, 546, 673
MflI RGATCY 3 cut(s) 92, 280, 707
MhlI GDGCHC 4 cut(s) 604, 648, 751, 762
MluCI AATT 3 cut(s) 434, 566, 664
MlyI GAGTC 4 cut(s) 124, 595, 704, 811
MmeI TCCRAC 3 cut(s) 211, 803, 803
MnlI CCTC 8 cut(s) 82, 187, 227, 381, 400, 451, 492, 723
Mph1103I ATGCAT 1 cut(s) 684
MroXI GAANNNNTTC 1 cut(s) 835
MseI TTAA 7 cut(s) 65, 101, 402, 419, 569, 617, 774
MslI CAYNNNNRTG 2 cut(s) 290, 607
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 599, 825
NcoI CCATGG 1 cut(s) 285
NdeII GATC 3 cut(s) 92, 280, 707
NlaIII CATG 3 cut(s) 289, 380, 562
NlaIV GGNNCC 2 cut(s) 346, 827
NmeAIII GCCGAG 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 835
PfeI GAWTC 4 cut(s) 56, 137, 410, 785
PflFI GACNNNGTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 4 cut(s) 123, 594, 703, 810
PpsI GAGTC 4 cut(s) 123, 594, 703, 810
PpuMI RGGWCCY 1 cut(s) 174
PshBI ATTAAT 1 cut(s) 569
PsiI TTATAA 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 346, 827
PspPI GGNCC 1 cut(s) 174
PspPPI RGGWCCY 1 cut(s) 174
PsuI RGATCY 3 cut(s) 92, 280, 707
PsyI GACNNNGTC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 5 cut(s) 70, 84, 447, 548, 735
RsaNI GTAC 5 cut(s) 69, 83, 446, 547, 734
RseI CAYNNNNRTG 2 cut(s) 290, 607
SaqAI TTAA 7 cut(s) 65, 101, 402, 419, 569, 617, 774
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 3 cut(s) 92, 280, 707
Sau96I GGNCC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 4 cut(s) 124, 595, 704, 811
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 4 cut(s) 604, 648, 751, 762
SfaNI GCATC 3 cut(s) 199, 523, 669
SinI GGWCC 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 290, 607
Sse9I AATT 3 cut(s) 434, 566, 664
SsiI CCGC 1 cut(s) 467
SspI AATATT 1 cut(s) 578
SspMI CTAG 2 cut(s) 105, 185
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 2 cut(s) 184, 285
TaaI ACNGT 2 cut(s) 323, 450
TaqI TCGA 2 cut(s) 408, 573
TasI AATT 3 cut(s) 434, 566, 664
TatI WGTACW 2 cut(s) 445, 733
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 4 cut(s) 56, 137, 410, 785
Tru1I TTAA 7 cut(s) 65, 101, 402, 419, 569, 617, 774
Tru9I TTAA 7 cut(s) 65, 101, 402, 419, 569, 617, 774
TscAI CASTG 3 cut(s) 609, 648, 799
TspDTI ATGAA 7 cut(s) 365, 468, 517, 642, 669, 714, 828
TspGWI ACGGA 2 cut(s) 728, 741
TspRI CASTG 3 cut(s) 609, 648, 799
Tth111I GACNNNGTC 1 cut(s) 391
VneI GTGCAC 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 174
VspI ATTAAT 1 cut(s) 569
XapI RAATTY 1 cut(s) 664
XmaJI CCTAGG 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 835
XspI CTAG 2 cut(s) 105, 185
ZrmI AGTACT 1 cut(s) 447
Zsp2I ATGCAT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.