pycom17g21890

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007750
Physical Location & Seq
Forward (+)
20319237 .. 20320877
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1641 bp
ATGATTCATGAGAAGGATGTTGCAACTTGGAATGTTATGATTTCTGGGTACAATCGAATCAAGAAGTTTGATGAATCCTTGAAGCTTTTTAGTGACATGGAGAAAACAGGAGTGTTGCCCACCTCAGTTACACTTGTCTCGGTGATATCAGCATGCTCCAAATTGAAGAATTTAGATGCCGGGAAGCAGGTTCATAAATACGTCAAGGATTGCCTGATCAAACCTGATTTGGTGTTGGGAAATGCTTTGGTTGATATGTATGTAGCTTGTGGGGAAATGCACGTTGCACTTGAGATTTTCGAAAAGATGAAAACAAAGGATGTAATTTCTTGGACTACCATTGTTAAGGGGTTTGCCAATTCTGGACAAGTTGATCTAGCTCGGAGCTACTTCGATGAGATGCCTGAAAGAGACTACATTTCATGGACTGCAATAATGGATGGGTACCTCCGGGTGAACCGGTTCAAAGAGGCACTGGAACTTTTCCGCCAGATGCAGACTTTGAATGTAAGGCCAGATGAGTACACTATGGTAAGCATTCTAACAGCCTGTGCACATCTAGGAGCACTTGAACTCGGAGAATGGATAAAAACTTACATTGACAAGAACAAGATCAAGAATGATGTATTCGTTCGAAATGCTTTAATAGACATGTATTTCAAGTGTGGAAATGTTGAGAAAGCTTTGAGGGTCTTTAATGCAATGCTTCAAAGGGACAAATTTACATGGACGGCTGTGATTGTTGGCCTTGCTGTTAACGGACATGGTAAGGAAGCTCTTGACGTGTTCTCTCAAATGGTAGATTCTTCGATAACTCCCGACAGGATAACTTTCATCGGTGTTCTTTCTGCGTGTACTCACTCTGGAATGGTAGCTGAAGGAAGGAACTTTTTCGATAGTATGACCACCCAACATGGAATCCAGCCTGAAGTTTCACATTACGGGTGCATGGTGGATCTTCTTGGCCGATCAGGGAATCTAAACGAAGCTTTTGAGGTTATACAGAACATGCCAATGAAACCAAATGCAGCTGTATGGGGAGCTCTGCTTGGCGCTTGTAGAATGCACAGAGATGCAGAACTGGCTGATATTGCAGCTAAACATATGCTCGAGTTGGAGCCCGACAACAGTTCTGTTTATGTTCTCTTGTGCAATATATACGCAGCTTGCAGTAGATGGGACAATTTGCGCAACGTAAGACAACTGATGATGGACAGGGGAATCAAGAAGACGCCTGGTTGCAGCTCGATAGAGGTGAATGGTAATGTTCATGAGTTTGTTGCCGGGGACCGGTCACACCCTCAATCCGAAAAAATCTATTCCAAGTTGGATAAGACGATTGAAGACTTGAAGGTTGTCGGGTATTCGCCGGATACATCGGAGGTTTTCCTTGATGTGGGGGAAGAGGATAAAGAGAGTGCAGTTTGCAGGCATAGTGAGAAGTTAGCAATGGCATTTGGGCTGATTAGTTTGGGAGATGGGGCGACAATTAGAATCATGAAGAACCTTAGAATCTGTGTGGATTGTCACAGCATGGCAAAGTTGGTCTCCAGAGTTTATGATAGAGAAGTGATTGTTAGGGATGGAACTCGGTTCCACCATTTTAGACGCGGTTCGTGTTCGTGTAAAGATTATTGGTGA

Protein Analysis

547

Amino Acids

61.51

Weight (kDa)

6.48

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 5 - 54 7.3e-12 PPR repeat family
PPR PF01535 9 - 38 1.6e-06 PPR repeat
TPR_24 PF23276 125 - 234 6.2e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 138 - 186 2.8e-12 PPR repeat family
PPR PF01535 140 - 169 3.6e-06 PPR repeat
PPR_2 PF13041 208 - 238 8.4e-06 PPR repeat family
PPR_2 PF13041 239 - 285 2e-08 PPR repeat family
E_motif PF20431 356 - 418 1e-19 E motif
Eplus_motif PF20430 423 - 445 2e-08 E+ motif
DYW_deaminase PF14432 454 - 546 2e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 178
Acc65I GGTACC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 444
AccII CGCG 1 cut(s) 1611
AciI CCGC 2 cut(s) 487, 1611
AclWI GGATC 1 cut(s) 963
AcoI YGGCCR 1 cut(s) 964
AcsI RAATTY 2 cut(s) 169, 719
AcuI CTGAAG 2 cut(s) 897, 948
AcyI GRCGYC 1 cut(s) 1232
AfaI GTAC 4 cut(s) 50, 446, 524, 856
AfiI CCNNNNNNNGG 2 cut(s) 1290, 1396
AflIII ACRYGT 2 cut(s) 651, 783
AgeI ACCGGT 2 cut(s) 459, 1290
AgsI TTSAA 9 cut(s) 82, 166, 466, 505, 572, 661, 710, 1343, 1351
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 1 cut(s) 1234
Alw21I GWGCWC 3 cut(s) 556, 568, 1045
Alw26I GTCTC 3 cut(s) 142, 405, 1552
Alw44I GTGCAC 1 cut(s) 552
AlwI GGATC 1 cut(s) 963
Ama87I CYCGRG 1 cut(s) 1109
AoxI GGCC 3 cut(s) 512, 745, 964
ApaLI GTGCAC 1 cut(s) 552
ApeKI GCWGC 4 cut(s) 1028, 1094, 1163, 1242
ApoI RAATTY 2 cut(s) 169, 719
AsiGI ACCGGT 2 cut(s) 459, 1290
Asp700I GAANNNNTTC 2 cut(s) 461, 890
Asp718I GGTACC 1 cut(s) 444
AspLEI GCGC 2 cut(s) 1055, 1191
AspS9I GGNCC 1 cut(s) 1288
AsuC2I CCSGG 3 cut(s) 181, 452, 1285
AsuHPI GGTGA 3 cut(s) 154, 466, 1267
AsuII TTCGAA 2 cut(s) 300, 634
AvaI CYCGRG 1 cut(s) 1109
AvaII GGWCC 1 cut(s) 1288
BaeGI GKGCMC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 444
BanII GRGCYC 2 cut(s) 1045, 1122
BbsI GAAGAC 2 cut(s) 1235, 1350
Bbv12I GWGCWC 3 cut(s) 556, 568, 1045
BbvI GCAGC 4 cut(s) 1040, 1106, 1175, 1254
BccI CCATC 5 cut(s) 434, 1170, 1204, 1472, 1577
BceAI ACGGC 1 cut(s) 747
BciT130I CCWGG 1 cut(s) 1236
BciVI GTATCC 1 cut(s) 1366
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 3 cut(s) 181, 452, 1285
BcoDI GTCTC 3 cut(s) 142, 405, 1552
BfaI CTAG 2 cut(s) 377, 560
BfoI RGCGCY 1 cut(s) 1056
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 1366
BisI GCNGC 4 cut(s) 1029, 1095, 1164, 1243
BlsI GCNGC 4 cut(s) 1030, 1096, 1165, 1244
Bme1390I CCNGG 4 cut(s) 181, 452, 1236, 1285
Bme18I GGWCC 1 cut(s) 1288
BmeT110I CYCGRG 1 cut(s) 1109
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 1 cut(s) 1288
BmiI GGNNCC 4 cut(s) 446, 1119, 1289, 1595
BmrFI CCNGG 4 cut(s) 181, 452, 1236, 1285
BmsI GCATC 4 cut(s) 166, 390, 483, 1063
BpiI GAAGAC 2 cut(s) 1235, 1350
BpmI CTGGAG 1 cut(s) 1534
Bpu14I TTCGAA 2 cut(s) 300, 634
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 3 cut(s) 181, 452, 1285
BsaHI GRCGYC 1 cut(s) 1232
BsaI GGTCTC 1 cut(s) 1552
BsaJI CCNNGG 1 cut(s) 1284
BsaWI WCCGGW 2 cut(s) 459, 1290
Bsc4I CCNNNNNNNGG 2 cut(s) 1290, 1396
Bse118I RCCGGY 2 cut(s) 459, 1290
Bse1I ACTGG 2 cut(s) 480, 1086
Bse3DI GCAATG 2 cut(s) 708, 1455
BseBI CCWGG 1 cut(s) 1236
BseDI CCNNGG 1 cut(s) 1284
BseGI GGATG 4 cut(s) 22, 325, 445, 1588
BseLI CCNNNNNNNGG 2 cut(s) 1290, 1396
BseMI GCAATG 2 cut(s) 708, 1455
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 2 cut(s) 480, 1086
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 4 cut(s) 1040, 1106, 1175, 1254
BsgI GTGCAG 1 cut(s) 1440
Bsh1236I CGCG 1 cut(s) 1611
BshFI GGCC 3 cut(s) 514, 747, 966
BshNI GGYRCC 1 cut(s) 444
BshTI ACCGGT 2 cut(s) 459, 1290
BsiHKAI GWGCWC 3 cut(s) 556, 568, 1045
BsiHKCI CYCGRG 1 cut(s) 1109
BsiSI CCGG 6 cut(s) 180, 451, 460, 1284, 1291, 1370
BslFI GGGAC 3 cut(s) 728, 1193, 1301
BslI CCNNNNNNNGG 2 cut(s) 1290, 1396
BsmAI GTCTC 3 cut(s) 142, 405, 1552
BsmFI GGGAC 3 cut(s) 728, 1193, 1301
BsmI GAATGC 2 cut(s) 537, 1068
BsnI GGCC 3 cut(s) 514, 747, 966
Bso31I GGTCTC 1 cut(s) 1552
BsoBI CYCGRG 1 cut(s) 1109
Bsp119I TTCGAA 2 cut(s) 300, 634
Bsp1286I GDGCHC 4 cut(s) 556, 568, 1045, 1122
Bsp143I GATC 5 cut(s) 216, 373, 612, 955, 968
BspACI CCGC 2 cut(s) 487, 1611
BspANI GGCC 3 cut(s) 514, 747, 966
BspCNI CTCAG 1 cut(s) 137
BspFNI CGCG 1 cut(s) 1611
BspHI TCATGA 3 cut(s) 7, 1270, 1497
BspLI GGNNCC 4 cut(s) 446, 1119, 1289, 1595
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 1 cut(s) 963
BspT104I TTCGAA 2 cut(s) 300, 634
BspT107I GGYRCC 1 cut(s) 444
BspTNI GGTCTC 1 cut(s) 1552
BsrDI GCAATG 2 cut(s) 708, 1455
BsrFI RCCGGY 2 cut(s) 459, 1290
BsrI ACTGG 2 cut(s) 480, 1086
BssAI RCCGGY 2 cut(s) 459, 1290
BssECI CCNNGG 1 cut(s) 1284
BssMI GATC 5 cut(s) 216, 373, 612, 955, 968
BssNI GRCGYC 1 cut(s) 1232
Bst2UI CCWGG 1 cut(s) 1236
Bst4CI ACNGT 1 cut(s) 1130
Bst6I CTCTTC 1 cut(s) 1398
BstACI GRCGYC 1 cut(s) 1232
BstBI TTCGAA 2 cut(s) 300, 634
BstC8I GCNNGC 3 cut(s) 154, 1168, 1430
BstDEI CTNAG 2 cut(s) 124, 1508
BstF5I GGATG 4 cut(s) 22, 325, 445, 1588
BstFNI CGCG 1 cut(s) 1611
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 2 cut(s) 1055, 1191
BstKTI GATC 5 cut(s) 219, 376, 615, 958, 971
BstMAI GTCTC 3 cut(s) 142, 405, 1552
BstMBI GATC 5 cut(s) 216, 373, 612, 955, 968
BstMWI GCNNNNNNNGC 2 cut(s) 1082, 1091
BstNI CCWGG 1 cut(s) 1236
BstNSI RCATGY 3 cut(s) 156, 655, 1012
BstSCI CCNGG 4 cut(s) 179, 450, 1234, 1283
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 1 cut(s) 1611
BstV1I GCAGC 4 cut(s) 1040, 1106, 1175, 1254
BstV2I GAAGAC 2 cut(s) 1235, 1350
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuI GTATCC 1 cut(s) 1366
BsuRI GGCC 3 cut(s) 514, 747, 966
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 4 cut(s) 22, 325, 445, 1588
BtsIMutI CAGTG 1 cut(s) 473
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 154, 1168, 1430
CciI TCATGA 3 cut(s) 7, 1270, 1497
CfoI GCGC 2 cut(s) 1055, 1191
Cfr10I RCCGGY 2 cut(s) 459, 1290
Cfr13I GGNCC 1 cut(s) 1288
CseI GACGC 2 cut(s) 1240, 1617
Csp6I GTAC 4 cut(s) 49, 445, 523, 855
CspAI ACCGGT 2 cut(s) 459, 1290
CviQI GTAC 4 cut(s) 49, 445, 523, 855
DdeI CTNAG 2 cut(s) 124, 1508
DpnI GATC 5 cut(s) 218, 375, 614, 957, 970
DpnII GATC 5 cut(s) 216, 373, 612, 955, 968
EaeI YGGCCR 1 cut(s) 964
Eam1104I CTCTTC 1 cut(s) 1398
EarI CTCTTC 1 cut(s) 1398
EciI GGCGGA 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 1043
Eco24I GRGCYC 2 cut(s) 1045, 1122
Eco31I GGTCTC 1 cut(s) 1552
Eco32I GATATC 1 cut(s) 147
Eco47I GGWCC 1 cut(s) 1288
Eco53kI GAGCTC 1 cut(s) 1043
Eco57I CTGAAG 2 cut(s) 897, 948
Eco88I CYCGRG 1 cut(s) 1109
EcoICRI GAGCTC 1 cut(s) 1043
EcoRII CCWGG 1 cut(s) 1234
EcoRV GATATC 1 cut(s) 147
EcoT38I GRGCYC 2 cut(s) 1045, 1122
FaqI GGGAC 3 cut(s) 728, 1193, 1301
FauNDI CATATG 1 cut(s) 1104
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 1029, 1095, 1164, 1243
FokI GGATG 4 cut(s) 29, 332, 452, 1595
FriOI GRGCYC 2 cut(s) 1045, 1122
Fsp4HI GCNGC 4 cut(s) 1029, 1095, 1164, 1243
FspBI CTAG 2 cut(s) 377, 560
FspI TGCGCA 1 cut(s) 1190
GlaI GCGC 2 cut(s) 1054, 1190
GluI GCNGC 4 cut(s) 1029, 1095, 1164, 1243
GsuI CTGGAG 1 cut(s) 1534
HaeII RGCGCY 1 cut(s) 1056
HaeIII GGCC 3 cut(s) 514, 747, 966
HapII CCGG 6 cut(s) 180, 451, 460, 1284, 1291, 1370
HgaI GACGC 2 cut(s) 1240, 1617
HhaI GCGC 2 cut(s) 1055, 1191
Hin1I GRCGYC 1 cut(s) 1232
Hin6I GCGC 2 cut(s) 1053, 1189
HinP1I GCGC 2 cut(s) 1053, 1189
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 3 cut(s) 83, 681, 987
HinfI GANTC 9 cut(s) 4, 57, 74, 803, 918, 976, 1221, 1494, 1512
HpaI GTTAAC 1 cut(s) 757
HpaII CCGG 6 cut(s) 180, 451, 460, 1284, 1291, 1370
HphI GGTGA 3 cut(s) 154, 466, 1267
Hpy166II GTNNAC 5 cut(s) 457, 525, 554, 757, 855
Hpy188I TCNGA 4 cut(s) 384, 578, 1309, 1381
Hpy8I GTNNAC 5 cut(s) 457, 525, 554, 757, 855
HpyAV CCTTC 4 cut(s) 7, 872, 876, 1345
HpyCH4III ACNGT 1 cut(s) 1130
HpyCH4IV ACGT 4 cut(s) 201, 282, 783, 1194
HpyF10VI GCNNNNNNNGC 2 cut(s) 1082, 1091
HpyF3I CTNAG 2 cut(s) 124, 1508
HpySE526I ACGT 4 cut(s) 201, 282, 783, 1194
Hsp92I GRCGYC 1 cut(s) 1232
HspAI GCGC 2 cut(s) 1053, 1189
KpnI GGTACC 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 216
KspAI GTTAAC 1 cut(s) 757
Kzo9I GATC 5 cut(s) 216, 373, 612, 955, 968
LmnI GCTCC 5 cut(s) 161, 384, 563, 1040, 1117
Lsp1109I GCAGC 4 cut(s) 1040, 1106, 1175, 1254
LweI GCATC 4 cut(s) 166, 390, 483, 1063
MaeI CTAG 2 cut(s) 377, 560
MaeII ACGT 4 cut(s) 201, 282, 783, 1194
MaeIII GTNAC 4 cut(s) 92, 127, 1293, 1526
MalI GATC 5 cut(s) 218, 375, 614, 957, 970
MboI GATC 5 cut(s) 216, 373, 612, 955, 968
MboII GAAGA 7 cut(s) 178, 798, 950, 1240, 1355, 1415, 1513
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 4 cut(s) 556, 568, 1045, 1122
MluCI AATT 7 cut(s) 161, 169, 324, 358, 719, 1183, 1488
MmeI TCCRAC 2 cut(s) 1095, 1308
MnlI CCTC 9 cut(s) 133, 458, 463, 681, 988, 1246, 1311, 1375, 1399
MroXI GAANNNNTTC 2 cut(s) 461, 890
MseI TTAA 4 cut(s) 345, 644, 696, 756
MslI CAYNNNNRTG 2 cut(s) 1013, 1071
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 6 cut(s) 180, 451, 460, 1284, 1291, 1370
MspR9I CCNGG 4 cut(s) 181, 452, 1236, 1285
Mva1269I GAATGC 2 cut(s) 537, 1068
MvaI CCWGG 1 cut(s) 1236
MvnI CGCG 1 cut(s) 1611
MwoI GCNNNNNNNGC 2 cut(s) 1082, 1091
NciI CCSGG 3 cut(s) 181, 452, 1285
NdeI CATATG 1 cut(s) 1104
NdeII GATC 5 cut(s) 216, 373, 612, 955, 968
NlaIV GGNNCC 4 cut(s) 446, 1119, 1289, 1595
NmuCI GTSAC 3 cut(s) 92, 1293, 1526
NsbI TGCGCA 1 cut(s) 1190
NspI RCATGY 3 cut(s) 156, 655, 1012
NspV TTCGAA 2 cut(s) 300, 634
PaeI GCATGC 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 1109
PagI TCATGA 3 cut(s) 7, 1270, 1497
PciI ACATGT 1 cut(s) 651
PctI GAATGC 2 cut(s) 537, 1068
PdmI GAANNNNTTC 2 cut(s) 461, 890
PfeI GAWTC 9 cut(s) 4, 57, 74, 803, 918, 976, 1221, 1494, 1512
PinAI ACCGGT 2 cut(s) 459, 1290
PkrI GCNGC 4 cut(s) 1030, 1096, 1165, 1244
PscI ACATGT 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 1045
Psp6I CCWGG 1 cut(s) 1234
PspGI CCWGG 1 cut(s) 1234
PspN4I GGNNCC 4 cut(s) 446, 1119, 1289, 1595
PspPI GGNCC 1 cut(s) 1288
PspXI VCTCGAGB 1 cut(s) 1109
PsuI RGATCY 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 50, 446, 524, 856
RsaNI GTAC 4 cut(s) 49, 445, 523, 855
RseI CAYNNNNRTG 2 cut(s) 1013, 1071
SacI GAGCTC 1 cut(s) 1045
SaqAI TTAA 4 cut(s) 345, 644, 696, 756
SatI GCNGC 4 cut(s) 1029, 1095, 1164, 1243
Sau3AI GATC 5 cut(s) 216, 373, 612, 955, 968
Sau96I GGNCC 1 cut(s) 1288
ScrFI CCNGG 4 cut(s) 181, 452, 1236, 1285
SduI GDGCHC 4 cut(s) 556, 568, 1045, 1122
SfaNI GCATC 4 cut(s) 166, 390, 483, 1063
Sfr274I CTCGAG 1 cut(s) 1109
SfuI TTCGAA 2 cut(s) 300, 634
SinI GGWCC 1 cut(s) 1288
SlaI CTCGAG 1 cut(s) 1109
SmiMI CAYNNNNRTG 2 cut(s) 1013, 1071
SmlI CTYRAG 2 cut(s) 290, 1109
SmoI CTYRAG 2 cut(s) 290, 1109
SphI GCATGC 1 cut(s) 156
Sse9I AATT 7 cut(s) 161, 169, 324, 358, 719, 1183, 1488
SsiI CCGC 2 cut(s) 487, 1611
SspMI CTAG 2 cut(s) 377, 560
SstI GAGCTC 1 cut(s) 1045
StyD4I CCNGG 4 cut(s) 179, 450, 1234, 1283
TaaI ACNGT 1 cut(s) 1130
TaiI ACGT 4 cut(s) 204, 285, 786, 1197
TaqI TCGA 8 cut(s) 55, 300, 393, 634, 809, 894, 1110, 1247
TasI AATT 7 cut(s) 161, 169, 324, 358, 719, 1183, 1488
TatI WGTACW 2 cut(s) 522, 854
TfiI GAWTC 9 cut(s) 4, 57, 74, 803, 918, 976, 1221, 1494, 1512
Tru1I TTAA 4 cut(s) 345, 644, 696, 756
Tru9I TTAA 4 cut(s) 345, 644, 696, 756
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 3 cut(s) 92, 1293, 1526
TseI GCWGC 4 cut(s) 1028, 1094, 1163, 1242
Tsp45I GTSAC 3 cut(s) 92, 1293, 1526
TspDTI ATGAA 8 cut(s) 87, 182, 323, 411, 823, 1031, 1259, 1514
TspGWI ACGGA 1 cut(s) 774
TspRI CASTG 1 cut(s) 480
VneI GTGCAC 1 cut(s) 552
VpaK11BI GGWCC 1 cut(s) 1288
XapI RAATTY 2 cut(s) 169, 719
XceI RCATGY 3 cut(s) 156, 655, 1012
XhoI CTCGAG 1 cut(s) 1109
XmnI GAANNNNTTC 2 cut(s) 461, 890
XspI CTAG 2 cut(s) 377, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.