pycom17g23790

Pentatricopeptide repeat-containing protein At2g13420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006370
Physical Location & Seq
Forward (+)
22088731 .. 22089417
687 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGATTTTTGCGGCCCAAAGTCGCGTCTGCTTATCCTTGCCTTGTTTCCAGTTATTCTCCTCTCTTGTTCGTTTCCCTTCAGTTCAAATCCATCGCTCCTGCTCTTCATTCCCCACCCTTAATGACCCAAATGGTGACACAAAGGACGCTAATGATGCTGAATTGATATCGAAAATCCTTGTCCACCACCATAATCCCTTCCACGCCATGGAGTCCTCTCTCCAACTCCATGGTATTACTCTCTCCTCCCAACTTGTCCACCACACCCTCCTCCGCCTCACCAATAATTCCAAAATTGCTCTTGCCTTCTTCCAGTATGCCAAGTCCCTTCCCAACCCTCCTCTCACCACAGCCTCCTTCAACCTGCTCATCGACATCCTTGCCAAGGTCCGTCAGTATGACGTCGCTTGGCAGCTCATCGTTGAAATGGACAACTTCAACCTCACTCCCACCACTGCCACATTTCTTACCCTCATTCGCCGCTTGATCGATCCTCTGGCCTCACCCGCCAAGCCGTTCGGGCCTTTGACGACATCGAGACCTTTGTCCAAACCAAGCCCAGTTGCCAAGATTTTTGCTTTTTGCTTGATACCCTTTGCAAATACGGTCATGTTAAGGTTGCAACTGAAGTTTTTAACAAAAGGAAGCACGAATTTGTCCCCGATGTCAAAATGTACACTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

229

Amino Acids

25.38

Weight (kDa)

9.63

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 117 - 162 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 208
AccII CGCG 1 cut(s) 24
AciI CCGC 4 cut(s) 11, 274, 481, 507
AclWI GGATC 1 cut(s) 485
AcsI RAATTY 1 cut(s) 652
AcuI CTGAAG 2 cut(s) 63, 647
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 2 cut(s) 208, 385
AgsI TTSAA 4 cut(s) 86, 361, 425, 439
AluBI AGCT 1 cut(s) 415
AluI AGCT 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 1 cut(s) 485
AoxI GGCC 3 cut(s) 12, 498, 521
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 1 cut(s) 652
AspS9I GGNCC 3 cut(s) 13, 388, 521
AsuHPI GGTGA 4 cut(s) 146, 271, 337, 495
AvaII GGWCC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 424
BccI CCATC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 499
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BcoDI GTCTC 1 cut(s) 532
BfuAI ACCTGC 1 cut(s) 372
BglI GCCNNNNNGGC 1 cut(s) 520
BisI GCNGC 3 cut(s) 12, 413, 481
BlsI GCNGC 3 cut(s) 13, 414, 482
Bme18I GGWCC 1 cut(s) 388
BmgT120I GGNCC 3 cut(s) 13, 388, 521
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 1 cut(s) 145
BmuI ACTGGG 1 cut(s) 554
BoxI GACNNNNGTC 1 cut(s) 544
Bsa29I ATCGAT 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 3 cut(s) 207, 229, 384
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 385
Bse1I ACTGG 3 cut(s) 49, 313, 560
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 3 cut(s) 207, 229, 384
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 208, 385
BseNI ACTGG 3 cut(s) 49, 313, 560
BseRI GAGGAG 4 cut(s) 49, 235, 260, 330
BseXI GCAGC 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 14, 500, 523
BshVI ATCGAT 1 cut(s) 489
BslFI GGGAC 2 cut(s) 310, 643
BslI CCNNNNNNNGG 2 cut(s) 208, 385
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 310, 643
BsnI GGCC 3 cut(s) 14, 500, 523
Bso31I GGTCTC 1 cut(s) 532
Bsp1407I TGTACA 1 cut(s) 674
Bsp143I GATC 2 cut(s) 486, 490
Bsp19I CCATGG 2 cut(s) 207, 229
BspACI CCGC 4 cut(s) 11, 274, 481, 507
BspANI GGCC 3 cut(s) 14, 500, 523
BspDI ATCGAT 1 cut(s) 489
BspFNI CGCG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 372
BspPI GGATC 1 cut(s) 485
BspQI GCTCTTC 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 532
BsrGI TGTACA 1 cut(s) 674
BsrI ACTGG 3 cut(s) 49, 313, 560
BssECI CCNNGG 3 cut(s) 207, 229, 384
BssMI GATC 2 cut(s) 486, 490
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 3 cut(s) 207, 229, 384
Bst4CI ACNGT 2 cut(s) 607, 681
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 402
BstAUI TGTACA 1 cut(s) 674
BstDSI CCRYGG 2 cut(s) 207, 229
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 375
BstFNI CGCG 1 cut(s) 24
BstKTI GATC 2 cut(s) 489, 493
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 2 cut(s) 486, 490
BstMWI GCNNNNNNNGC 3 cut(s) 155, 506, 520
BstPAI GACNNNNGTC 1 cut(s) 544
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 230
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 3 cut(s) 14, 500, 523
BsuTUI ATCGAT 1 cut(s) 489
BtgI CCRYGG 2 cut(s) 207, 229
BtgZI GCGATG 1 cut(s) 77
BtsCI GGATG 1 cut(s) 375
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 453, 677
BveI ACCTGC 1 cut(s) 372
Cfr13I GGNCC 3 cut(s) 13, 388, 521
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 2 cut(s) 13, 155
Csp6I GTAC 1 cut(s) 675
CviAII CATG 3 cut(s) 208, 230, 610
CviJI RGCY 7 cut(s) 14, 353, 415, 500, 514, 523, 558
CviKI_1 RGCY 7 cut(s) 14, 353, 415, 500, 514, 523, 558
CviQI GTAC 1 cut(s) 675
DpnI GATC 2 cut(s) 488, 492
DpnII GATC 2 cut(s) 486, 490
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 263
Eco130I CCWWGG 3 cut(s) 207, 229, 384
Eco31I GGTCTC 1 cut(s) 532
Eco32I GATATC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 388
Eco57I CTGAAG 2 cut(s) 63, 647
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoRV GATATC 1 cut(s) 168
EcoT14I CCWWGG 3 cut(s) 207, 229, 384
ErhI CCWWGG 3 cut(s) 207, 229, 384
FaeI CATG 3 cut(s) 211, 233, 613
FaiI YATR 6 cut(s) 192, 209, 231, 318, 399, 611
FaqI GGGAC 2 cut(s) 310, 643
FatI CATG 3 cut(s) 207, 229, 609
FauI CCCGC 1 cut(s) 514
Fnu4HI GCNGC 3 cut(s) 12, 413, 481
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 3 cut(s) 12, 413, 481
GluI GCNGC 3 cut(s) 12, 413, 481
HaeIII GGCC 3 cut(s) 14, 500, 523
HgaI GACGC 2 cut(s) 13, 155
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 3 cut(s) 211, 233, 613
HinfI GANTC 1 cut(s) 212
HphI GGTGA 4 cut(s) 146, 271, 337, 495
Hpy166II GTNNAC 3 cut(s) 184, 259, 677
Hpy188III TCNNGA 1 cut(s) 537
Hpy8I GTNNAC 3 cut(s) 184, 259, 677
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 5 cut(s) 87, 208, 316, 338, 367
HpyCH4III ACNGT 2 cut(s) 607, 681
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 2 cut(s) 599, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 506, 520
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 3 cut(s) 211, 233, 613
Kzo9I GATC 2 cut(s) 486, 490
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 6 cut(s) 62, 112, 326, 377, 482, 573
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 1 cut(s) 145
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 488, 492
MboI GATC 2 cut(s) 486, 490
MboII GAAGA 2 cut(s) 96, 301
MluCI AATT 4 cut(s) 161, 286, 294, 652
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 247
MseI TTAA 3 cut(s) 120, 614, 635
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 155, 506, 520
NcoI CCATGG 2 cut(s) 207, 229
NdeII GATC 2 cut(s) 486, 490
NlaIII CATG 3 cut(s) 211, 233, 613
NmuCI GTSAC 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 109
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 3 cut(s) 13, 414, 482
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PshAI GACNNNNGTC 1 cut(s) 544
PspPI GGNCC 3 cut(s) 13, 388, 521
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 120, 614, 635
SatI GCNGC 3 cut(s) 12, 413, 481
Sau3AI GATC 2 cut(s) 486, 490
Sau96I GGNCC 3 cut(s) 13, 388, 521
SchI GAGTC 1 cut(s) 221
SetI ASST 7 cut(s) 366, 390, 405, 417, 444, 544, 620
SfaNI GCATC 1 cut(s) 145
SinI GGWCC 1 cut(s) 388
Sse9I AATT 4 cut(s) 161, 286, 294, 652
SsiI CCGC 4 cut(s) 11, 274, 481, 507
StyI CCWWGG 3 cut(s) 207, 229, 384
TaaI ACNGT 2 cut(s) 607, 681
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 170, 372, 489, 536
TasI AATT 4 cut(s) 161, 286, 294, 652
TatI WGTACW 1 cut(s) 674
TauI GCSGC 2 cut(s) 14, 483
Tru1I TTAA 3 cut(s) 120, 614, 635
Tru9I TTAA 3 cut(s) 120, 614, 635
TscAI CASTG 2 cut(s) 460, 684
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 460, 684
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 388
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 652
ZraI GACGTC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.