Rh3CG286300

Pentatricopeptide repeat-containing protein At2g13420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0006370
Physical Location & Seq
Forward (+)
28942840 .. 28944213
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG286300.1

Sequence Viewer

Length: 1374 bp
ATGGAGTCCTCTCTCCAGCTCCACGGCATCACTCTCTCCCCCGACCTCCTCCACCACACTCTCCTCCGCCTCAAGCACAATTCCAAAATCGCCCTCGCCCTCTTCCACTACGCCAAGTCCCTCCCCTCCCCCCCGCCCCTCACCTCCCACGCCTTCCACCTCCTCATCGACATCGTCGCCAAGGTCCGCCAGTACGACGTCGCTTGGCAGCTCATCCTCGAAATGGACCACCACAACCTCACCCCCACCGCCGCCACCTTCCTCATCCTCATCCGCCGCCTCATCGCCTCCGGCCTCACCCGCCAGGCCGTCCGGGCCTTCGACGACATCGAGACCTTCGTCCTGATCCGCCCCACCGCCCAAGACTTCTGCTCTCTACTCGACACCCTCTGTAAATACGGCTACGTTAAGGTCGCGGCTGAAGTTTTCAACAAAAGGAAGCATGACTATGTGGCCGATGTGAAAATGTACACTGTTCTGGTTTACGGGTGGTGTAAGATCGGCCGTTTCGATATGGCGGAGAGGTTCTTGAGGGATATGATTGAGCGTGGTATTGAGCCCAATGTGGTTACTTACAATGTGTTTCTGGATGGGATTTGTAGGAAGGCGAGTTTGCACCCACAGGACAGGTTTGAGAGGACCATAAGAAATGCACTGAAGGTGTTCGACGAAATGTCCAGCAGAGGCATTGAGCCGGACGTGACGAGTTTTTCGATCCTTCTTCATGTTTATAGCCGGGCGCATAAGCCCGAGTTGTCGCTTGATAAGCTCAAGGAAATGAGGGAGAGGGGGATTTGCCCCACCGTGGCGACTTACACTTCAGTTGTCAAGTGTCTCTGCTCTTGCGGGAGGCTTGAGGAGGCGGAGGAGTTGCTTGGTGAGATGGTGAGGAATGGGGTTAGTCCCTCTGCGGCTACGTATAATTGTTTCTTCAAAGAGTACAGGGGGAGAAAGGACTCGGAAAGTGCTTTGAAGTTGTATAGGAAGATGAAGGAGGATAGATTGTTTGCGCCGAGTGTGCACACTTATAATATATTGGTGGGGATGTTTTTGCAGTTGAATCGAGTGGAGATTGTGAGAGAGATATGGGATGACATGAAAGAGAGCGGGGCGGGGCCGGATTTGGATTCGTATACGATGTTGATTCATGGGCAATGTGGGAAGCAGAAGTGGAGAGAGGCGTGCCAGTTTTTTGTGGAGATGATAGAGAAGGGGCTTCTTCCTCAGAAAGTTACTTTTGAGACGCTTTACAGAGGGTTAATACAGTCTGATATGTTGAGAACTTGGAGAAGATTGAAGAAGAAGCTCGATGAGGAGTCTGTTACTTTTGGATCCGAGTTTCAAAATTATCACCTTAAGCCATACAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

457

Amino Acids

52.95

Weight (kDa)

8.87

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 152 - 197 8.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 153 - 201 7.4e-14 PPR repeat family
PPR_long PF17177 179 - 308 2.1e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 215 - 244 4.9e-07 PPR repeat family
PPR_3 PF13812 220 - 277 9.4e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 237 - 280 8e-08 PPR repeat family
TPR_24 PF23276 241 - 355 1.1e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 263 - 296 2.9e-11 PPR repeat
PPR_2 PF13041 271 - 313 7.5e-11 PPR repeat family
PPR PF01535 271 - 300 1.7e-08 PPR repeat
PPR_3 PF13812 291 - 350 3.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 306 - 350 1.3e-07 PPR repeat family
PPR_2 PF13041 338 - 386 2.9e-10 PPR repeat family
PPR_2 PF13041 373 - 420 4.5e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
AatII GACGTC 1 cut(s) 201
AccBSI CCGCTC 1 cut(s) 1107
AccI GTMKAC 1 cut(s) 1133
AccII CGCG 1 cut(s) 416
AclWI GGATC 4 cut(s) 340, 709, 1326, 1339
AcoI YGGCCR 2 cut(s) 453, 502
AcuI CTGAAG 3 cut(s) 441, 677, 804
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 3 cut(s) 194, 470, 941
AfiI CCNNNNNNNGG 2 cut(s) 223, 805
AflII CTTAAG 1 cut(s) 1355
AgsI TTSAA 6 cut(s) 430, 934, 973, 1060, 1297, 1343
AhdI GACNNNNNGTC 1 cut(s) 673
AjiI CACGTC 1 cut(s) 700
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 4 cut(s) 19, 211, 769, 1306
AluI AGCT 4 cut(s) 19, 211, 769, 1306
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 3 cut(s) 326, 839, 1235
Alw44I GTGCAC 1 cut(s) 1019
AlwI GGATC 4 cut(s) 340, 709, 1326, 1339
Ama87I CYCGRG 1 cut(s) 749
AoxI GGCC 6 cut(s) 292, 306, 315, 453, 502, 1115
ApaLI GTGCAC 1 cut(s) 1019
ApeKI GCWGC 1 cut(s) 208
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
Asp700I GAANNNNTTC 1 cut(s) 662
AspLEI GCGC 2 cut(s) 742, 1012
AspS9I GGNCC 5 cut(s) 184, 226, 315, 639, 1115
AsuC2I CCSGG 2 cut(s) 314, 737
AsuHPI GGTGA 6 cut(s) 133, 232, 289, 890, 898, 1343
AvaI CYCGRG 1 cut(s) 749
AvaII GGWCC 3 cut(s) 184, 226, 639
BaeGI GKGCMC 1 cut(s) 1023
BamHI GGATCC 1 cut(s) 1331
BanII GRGCYC 1 cut(s) 561
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 1 cut(s) 220
BccI CCATC 2 cut(s) 584, 877
BceAI ACGGC 4 cut(s) 40, 293, 415, 489
BcgI CGANNNNNNTGC 2 cut(s) 1043, 1077
BciT130I CCWGG 1 cut(s) 305
BcnI CCSGG 2 cut(s) 314, 737
BcoDI GTCTC 3 cut(s) 326, 839, 1235
BfrI CTTAAG 1 cut(s) 1355
BglI GCCNNNNNGGC 1 cut(s) 314
BisI GCNGC 5 cut(s) 209, 252, 277, 417, 912
BlsI GCNGC 5 cut(s) 210, 253, 278, 418, 913
Bme1390I CCNGG 3 cut(s) 305, 314, 737
Bme18I GGWCC 3 cut(s) 184, 226, 639
BmeRI GACNNNNNGTC 1 cut(s) 673
BmeT110I CYCGRG 1 cut(s) 749
BmgBI CACGTC 1 cut(s) 700
BmgT120I GGNCC 5 cut(s) 184, 226, 315, 639, 1115
BmiI GGNNCC 2 cut(s) 1116, 1333
BmrFI CCNGG 3 cut(s) 305, 314, 737
BmsI GCATC 1 cut(s) 36
BoxI GACNNNNGTC 1 cut(s) 338
BpuEI CTTGAG 4 cut(s) 56, 550, 755, 875
BpuMI CCSGG 2 cut(s) 314, 737
BsaAI YACGTR 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 22, 180, 804
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 805
Bse1I ACTGG 2 cut(s) 190, 1186
Bse3DI GCAATG 1 cut(s) 1160
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 22, 180, 804
BseGI GGATG 6 cut(s) 213, 264, 270, 595, 1050, 1096
BseLI CCNNNNNNNGG 2 cut(s) 223, 805
BseMI GCAATG 1 cut(s) 1160
BseMII CTCAG 1 cut(s) 1238
BseNI ACTGG 2 cut(s) 190, 1186
BseRI GAGGAG 6 cut(s) 38, 53, 152, 872, 881, 1328
BseSI GKGCMC 1 cut(s) 1023
BseX3I CGGCCG 1 cut(s) 502
BseXI GCAGC 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 416
Bsh1285I CGRYCG 1 cut(s) 505
BshFI GGCC 6 cut(s) 294, 308, 317, 455, 504, 1117
BsiEI CGRYCG 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 5 cut(s) 291, 313, 695, 736, 1118
BslFI GGGAC 2 cut(s) 103, 888
BslI CCNNNNNNNGG 2 cut(s) 223, 805
BsmAI GTCTC 3 cut(s) 326, 839, 1235
BsmBI CGTCTC 1 cut(s) 1235
BsmFI GGGAC 2 cut(s) 103, 888
BsnI GGCC 6 cut(s) 294, 308, 317, 455, 504, 1117
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 749
Bsp1286I GDGCHC 2 cut(s) 561, 1023
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 4 cut(s) 345, 498, 714, 1331
BspANI GGCC 6 cut(s) 294, 308, 317, 455, 504, 1117
BspCNI CTCAG 1 cut(s) 1237
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 1116, 1333
BspPI GGATC 4 cut(s) 340, 709, 1326, 1339
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 1107
BsrDI GCAATG 1 cut(s) 1160
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 2 cut(s) 190, 1186
BssECI CCNNGG 3 cut(s) 22, 180, 804
BssMI GATC 4 cut(s) 345, 498, 714, 1331
BssNAI GTATAC 1 cut(s) 1134
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 180
Bst1107I GTATAC 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 475, 805, 1266
Bst6I CTCTTC 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 1355
BstAUI TGTACA 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 1183
BstDEI CTNAG 1 cut(s) 1224
BstDSI CCRYGG 2 cut(s) 22, 804
BstF5I GGATG 6 cut(s) 213, 264, 270, 595, 1050, 1096
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 2 cut(s) 742, 1012
BstKTI GATC 4 cut(s) 348, 501, 717, 1334
BstMAI GTCTC 3 cut(s) 326, 839, 1235
BstMBI GATC 4 cut(s) 345, 498, 714, 1331
BstMCI CGRYCG 1 cut(s) 505
BstMWI GCNNNNNNNGC 4 cut(s) 300, 314, 766, 1018
BstNI CCWGG 1 cut(s) 305
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 3 cut(s) 303, 312, 735
BstSLI GKGCMC 1 cut(s) 1023
BstSNI TACGTA 1 cut(s) 918
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 1331
BstYI RGATCY 1 cut(s) 1331
BstZ17I GTATAC 1 cut(s) 1134
BstZI CGGCCG 1 cut(s) 502
BsuRI GGCC 6 cut(s) 294, 308, 317, 455, 504, 1117
BtgI CCRYGG 2 cut(s) 22, 804
BtgZI GCGATG 1 cut(s) 268
BtrI CACGTC 1 cut(s) 700
BtsCI GGATG 6 cut(s) 213, 264, 270, 595, 1050, 1096
BtsIMutI CAGTG 2 cut(s) 471, 653
Cac8I GCNNGC 1 cut(s) 1183
CfoI GCGC 2 cut(s) 742, 1012
Cfr13I GGNCC 5 cut(s) 184, 226, 315, 639, 1115
CseI GACGC 1 cut(s) 1252
Csp6I GTAC 3 cut(s) 193, 469, 940
CviAII CATG 4 cut(s) 443, 725, 1096, 1148
CviQI GTAC 3 cut(s) 193, 469, 940
DdeI CTNAG 1 cut(s) 1224
DpnI GATC 4 cut(s) 347, 500, 716, 1333
DpnII GATC 4 cut(s) 345, 498, 714, 1331
DriI GACNNNNNGTC 1 cut(s) 673
EaeI YGGCCR 2 cut(s) 453, 502
EagI CGGCCG 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 107
Eam1105I GACNNNNNGTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 107
EciI GGCGGA 6 cut(s) 56, 176, 263, 338, 533, 878
EclXI CGGCCG 1 cut(s) 502
Eco105I TACGTA 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 561
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 3 cut(s) 184, 226, 639
Eco52I CGGCCG 1 cut(s) 502
Eco57I CTGAAG 3 cut(s) 441, 677, 804
Eco88I CYCGRG 1 cut(s) 749
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 180
Esp3I CGTCTC 1 cut(s) 1235
FaeI CATG 4 cut(s) 446, 728, 1099, 1151
FaqI GGGAC 2 cut(s) 103, 888
FatI CATG 4 cut(s) 442, 724, 1095, 1147
FauI CCCGC 5 cut(s) 141, 308, 839, 1100, 1105
FblI GTMKAC 1 cut(s) 1133
Fnu4HI GCNGC 5 cut(s) 209, 252, 277, 417, 912
FokI GGATG 6 cut(s) 200, 251, 257, 602, 1057, 1103
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 5 cut(s) 209, 252, 277, 417, 912
GlaI GCGC 2 cut(s) 741, 1011
GluI GCNGC 5 cut(s) 209, 252, 277, 417, 912
HaeIII GGCC 6 cut(s) 294, 308, 317, 455, 504, 1117
HapII CCGG 5 cut(s) 291, 313, 695, 736, 1118
HgaI GACGC 1 cut(s) 1252
HhaI GCGC 2 cut(s) 742, 1012
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 4 cut(s) 446, 728, 1099, 1151
Hin6I GCGC 2 cut(s) 740, 1010
HinP1I GCGC 2 cut(s) 740, 1010
HinfI GANTC 6 cut(s) 5, 956, 1060, 1127, 1144, 1316
HpaII CCGG 5 cut(s) 291, 313, 695, 736, 1118
HphI GGTGA 6 cut(s) 133, 232, 289, 890, 898, 1343
Hpy166II GTNNAC 4 cut(s) 471, 484, 1021, 1134
Hpy188I TCNGA 4 cut(s) 961, 1227, 1270, 1336
Hpy188III TCNNGA 4 cut(s) 331, 343, 529, 587
Hpy8I GTNNAC 4 cut(s) 471, 484, 1021, 1134
Hpy99I CGWCG 5 cut(s) 179, 200, 203, 326, 671
HpyAV CCTTC 9 cut(s) 163, 268, 328, 346, 598, 652, 728, 985, 1204
HpyCH4III ACNGT 3 cut(s) 475, 805, 1266
HpyCH4IV ACGT 4 cut(s) 198, 405, 699, 917
HpyCH4V TGCA 4 cut(s) 616, 653, 1021, 1054
HpyF10VI GCNNNNNNNGC 4 cut(s) 300, 314, 766, 1018
HpyF3I CTNAG 1 cut(s) 1224
HpySE526I ACGT 4 cut(s) 198, 405, 699, 917
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 4 cut(s) 446, 728, 1099, 1151
HspAI GCGC 2 cut(s) 740, 1010
Kzo9I GATC 4 cut(s) 345, 498, 714, 1331
LmnI GCTCC 1 cut(s) 24
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 36
MaeII ACGT 4 cut(s) 198, 405, 699, 917
MaeIII GTNAC 4 cut(s) 568, 700, 1231, 1321
MalI GATC 4 cut(s) 347, 500, 716, 1333
MbiI CCGCTC 1 cut(s) 1107
MboI GATC 4 cut(s) 345, 498, 714, 1331
MboII GAAGA 8 cut(s) 94, 713, 922, 997, 1211, 1302, 1309, 1312
MflI RGATCY 1 cut(s) 1331
MhlI GDGCHC 2 cut(s) 561, 1023
MluCI AATT 3 cut(s) 79, 922, 1345
MlyI GAGTC 3 cut(s) 14, 950, 1325
MroXI GAANNNNTTC 1 cut(s) 662
MseI TTAA 3 cut(s) 408, 1259, 1356
MslI CAYNNNNRTG 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 5 cut(s) 291, 313, 695, 736, 1118
MspR9I CCNGG 3 cut(s) 305, 314, 737
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 4 cut(s) 300, 314, 766, 1018
NciI CCSGG 2 cut(s) 314, 737
NdeII GATC 4 cut(s) 345, 498, 714, 1331
NlaIII CATG 4 cut(s) 446, 728, 1099, 1151
NlaIV GGNNCC 2 cut(s) 1116, 1333
NmeAIII GCCGAG 1 cut(s) 1038
NmuCI GTSAC 1 cut(s) 700
PcsI WCGNNNNNNNCGW 4 cut(s) 327, 336, 507, 710
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 3 cut(s) 1060, 1127, 1144
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 5 cut(s) 210, 253, 278, 418, 913
PleI GAGTC 3 cut(s) 13, 950, 1324
PpsI GAGTC 3 cut(s) 13, 950, 1324
Ppu21I YACGTR 1 cut(s) 918
PshAI GACNNNNGTC 1 cut(s) 338
PsiI TTATAA 1 cut(s) 1029
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 1116, 1333
PspPI GGNCC 5 cut(s) 184, 226, 315, 639, 1115
PsuI RGATCY 1 cut(s) 1331
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 3 cut(s) 194, 470, 941
RsaNI GTAC 3 cut(s) 193, 469, 940
RseI CAYNNNNRTG 1 cut(s) 447
SaqAI TTAA 3 cut(s) 408, 1259, 1356
SatI GCNGC 5 cut(s) 209, 252, 277, 417, 912
Sau3AI GATC 4 cut(s) 345, 498, 714, 1331
Sau96I GGNCC 5 cut(s) 184, 226, 315, 639, 1115
SchI GAGTC 3 cut(s) 14, 950, 1325
ScrFI CCNGG 3 cut(s) 305, 314, 737
SduI GDGCHC 2 cut(s) 561, 1023
SfaNI GCATC 1 cut(s) 36
SfiI GGCCNNNNNGGCC 1 cut(s) 314
SinI GGWCC 3 cut(s) 184, 226, 639
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 5 cut(s) 71, 529, 770, 854, 1355
SmoI CTYRAG 5 cut(s) 71, 529, 770, 854, 1355
SnaBI TACGTA 1 cut(s) 918
Sse9I AATT 3 cut(s) 79, 922, 1345
StyD4I CCNGG 3 cut(s) 303, 312, 735
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 475, 805, 1266
TaiI ACGT 4 cut(s) 201, 408, 702, 920
TasI AATT 3 cut(s) 79, 922, 1345
TatI WGTACW 2 cut(s) 468, 939
TauI GCSGC 4 cut(s) 254, 279, 419, 914
TfiI GAWTC 3 cut(s) 1060, 1127, 1144
Tru1I TTAA 3 cut(s) 408, 1259, 1356
Tru9I TTAA 3 cut(s) 408, 1259, 1356
TscAI CASTG 2 cut(s) 478, 660
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 1 cut(s) 208
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 4 cut(s) 713, 1004, 1112, 1136
TspRI CASTG 2 cut(s) 478, 660
Tth111I GACNNNGTC 1 cut(s) 173
Vha464I CTTAAG 1 cut(s) 1355
VneI GTGCAC 1 cut(s) 1019
VpaK11BI GGWCC 3 cut(s) 184, 226, 639
XmiI GTMKAC 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 662
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.