Rroxscaffold_6G00398650

Pentatricopeptide repeat-containing protein At2g13420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006370
Physical Location & Seq
Reverse (-)
20526382 .. 20530770
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398650.1

Sequence Viewer

Length: 1509 bp
ATGTCGCTTCACCTCCTCTCTTCTTCTTCTCCGTTCAGCTCGCCGGTTCTCCTCACTTCCCAACCCAACAACAACCCAAAACGACGCCGAACTGGTATCAAACATCCTTCTCCACCACCACAACCCCTTCCACGCCATGGAGTCCTCTCTCCAGCTCCACGGCATCACTCTCTCCCCCGACCTCCTCCACCACACTCTCCTCCGCCTCAAGCACAATTCCAAAATCGCCCTCGCCCTCTTCCACTACGCCAAGTCCCTCCCCTCCCCCCGCCCCTCACCTCCGACGCCTTCCACCTCCTCATCGACATCGTCGCCAAGGTCCGCCAGTACGACGTCGCTTGGCAGCTCATCCTCGAAATGGACCGCCACAACCTCACCCCCACCGCCGCCACCTTCCTCATCCTCATCCGCCGCCTCATCGCCTCCGGCCTCACCCGCCAGGCCGTCCGGGCCTTCGACGACATCGAGACCTTCGTCCTGACCCGCCCCACCGCCCAAGACTTCTGCTTTCTACTCGACACCCTCTGTAAATACGGCTACGTAAAGCTCGCGGCTGAAGTTTTCAACAAGAGGAAGCATGACTATGTGGCCGATGTGAAAATGTACACTGTTCTGGTTTACGGGTGGTGTAAGATCGGCCGTTTCGATATGGCGGAGAGGTTCTTGAGGGATATGATCGAGCGAGGTATTGAGCCCAATGTGGTTACTTACAATGTGTTTCTGGATGGGATTTGTAGGAAGGCGAGTTTGCACCCTCAGGACAGGTTTGAGAGGACCATAAGAAATGCACTGAAGGTGTTCGATGAAATGTCCAGCAGAGGCATTGAGCCGGACGTGACTAGTTTTTCGATCCTTCTTCATGTTTATAGCCGGGCGCATAAGCCCGAGTTGTCGCTTGATAAGCTCAAGGAAATGAGGGAGAGGGGGATTTGCCCCACCGTGGCGACGTACACTTCAGTTGTCAAGTGTCTCTGCTCTTGCGGGAGGCTTGAGGAAGCGGAGGAGTTGCTTGGTGAGATGGTGGGGAATGGGGTTAGTCCCTCTGCGGCTACGTATAATTGTTTCTTCAAAGAGTACAGGGGGAGAAAGGACTCGGAAAGTGCTTTGAAGTTGTATCGGAAGATGAAGGAGGATAGATTGTTTGCACCGAGTGTGCACACTTATAATATTTTGGTGGGGATGTTTTTGCAGCTGAATCGAGTGGAGATTGTGAGAGAGATATGGGATGACATGAAGGAGAGCGGGGCAGGGCCGGATTTGGATTCATATACGATGTTGGTTCATGGGTTATGTGAGAAGCAGAAGTGGAGAGAGGCGTGCCAGTTTTTTGTGGAGATGATAGAGAAGGGGCTTCTTCCTCAGAAAGTTACTTTTGAGACACTCTACAGAGGGTTAATACAGTCTGATATGTTGAGAACTTGGAGAAGATTGAAGAAGAAGCTCGATGAAGAGTCTGTTACTTTTGGATCGGAGTTCCAAAATTATCACCTTAAGCCATACAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

502

Amino Acids

57.86

Weight (kDa)

9.3

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 197 - 242 8.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 198 - 246 8.4e-14 PPR repeat family
PPR_long PF17177 224 - 353 8.5e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 260 - 289 5.5e-07 PPR repeat family
PPR_3 PF13812 265 - 322 1e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 282 - 325 9.1e-08 PPR repeat family
TPR_24 PF23276 286 - 400 4.9e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 308 - 341 3.3e-11 PPR repeat
PPR_2 PF13041 316 - 358 7.5e-10 PPR repeat family
PPR PF01535 316 - 345 6.8e-08 PPR repeat
PPR_3 PF13812 336 - 395 4.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 351 - 395 2.7e-07 PPR repeat family
PPR_2 PF13041 383 - 431 4.7e-11 PPR repeat family
PPR_2 PF13041 418 - 465 3.2e-09 PPR repeat family
PPR_1 PF12854 418 - 446 3.8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AatII GACGTC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 137
AccBSI CCGCTC 1 cut(s) 1242
AccII CGCG 1 cut(s) 551
AclWI GGATC 2 cut(s) 844, 1474
AcoI YGGCCR 2 cut(s) 588, 637
AcuI CTGAAG 3 cut(s) 576, 812, 939
AcyI GRCGYC 3 cut(s) 85, 285, 333
AdeI CACNNNGTG 1 cut(s) 1151
AfaI GTAC 4 cut(s) 329, 605, 950, 1076
AfiI CCNNNNNNNGG 3 cut(s) 137, 358, 940
AflII CTTAAG 1 cut(s) 1490
AgsI TTSAA 4 cut(s) 565, 1069, 1108, 1432
AhlI ACTAGT 1 cut(s) 839
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 7 cut(s) 39, 155, 346, 547, 904, 1192, 1441
AluI AGCT 7 cut(s) 39, 155, 346, 547, 904, 1192, 1441
Alw21I GWGCWC 1 cut(s) 1158
Alw26I GTCTC 3 cut(s) 461, 974, 1370
Alw44I GTGCAC 1 cut(s) 1154
AlwI GGATC 2 cut(s) 844, 1474
Ama87I CYCGRG 1 cut(s) 884
AoxI GGCC 6 cut(s) 427, 441, 450, 588, 637, 1250
ApaLI GTGCAC 1 cut(s) 1154
ApeKI GCWGC 2 cut(s) 343, 1189
ArsI GACNNNNNNTTYG 2 cut(s) 829, 861
Asp700I GAANNNNTTC 1 cut(s) 797
AspLEI GCGC 1 cut(s) 877
AspS9I GGNCC 5 cut(s) 319, 361, 450, 774, 1250
AsuC2I CCSGG 2 cut(s) 449, 872
AsuHPI GGTGA 5 cut(s) 268, 367, 424, 1025, 1478
AvaI CYCGRG 1 cut(s) 884
AvaII GGWCC 3 cut(s) 319, 361, 774
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 1158
BanII GRGCYC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 1158
BbvI GCAGC 2 cut(s) 355, 1201
BccI CCATC 2 cut(s) 719, 1012
BceAI ACGGC 4 cut(s) 176, 428, 550, 624
BcgI CGANNNNNNTGC 2 cut(s) 1178, 1212
BciT130I CCWGG 1 cut(s) 440
BcnI CCSGG 2 cut(s) 449, 872
BcoDI GTCTC 3 cut(s) 461, 974, 1370
BcuI ACTAGT 1 cut(s) 839
BfaI CTAG 1 cut(s) 840
BfmI CTRYAG 1 cut(s) 1384
BfrI CTTAAG 1 cut(s) 1490
BglI GCCNNNNNGGC 1 cut(s) 449
BisI GCNGC 6 cut(s) 344, 387, 412, 552, 1047, 1190
BlsI GCNGC 6 cut(s) 345, 388, 413, 553, 1048, 1191
Bme1390I CCNGG 3 cut(s) 440, 449, 872
Bme18I GGWCC 3 cut(s) 319, 361, 774
BmeT110I CYCGRG 1 cut(s) 884
BmgBI CACGTC 1 cut(s) 835
BmgT120I GGNCC 5 cut(s) 319, 361, 450, 774, 1250
BmrFI CCNGG 3 cut(s) 440, 449, 872
BmsI GCATC 1 cut(s) 172
BoxI GACNNNNGTC 1 cut(s) 473
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 4 cut(s) 192, 685, 890, 1010
BpuMI CCSGG 2 cut(s) 449, 872
BsaAI YACGTR 2 cut(s) 541, 1053
BsaHI GRCGYC 3 cut(s) 85, 285, 333
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 4 cut(s) 136, 158, 315, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 358, 940
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 97, 325, 1321
Bse21I CCTNAGG 1 cut(s) 756
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 4 cut(s) 136, 158, 315, 939
BseGI GGATG 7 cut(s) 103, 348, 399, 405, 730, 1185, 1231
BseLI CCNNNNNNNGG 3 cut(s) 137, 358, 940
BseMII CTCAG 2 cut(s) 770, 1373
BseNI ACTGG 3 cut(s) 97, 325, 1321
BseRI GAGGAG 6 cut(s) 5, 41, 174, 189, 287, 1016
BseSI GKGCMC 1 cut(s) 1158
BseX3I CGGCCG 1 cut(s) 637
BseXI GCAGC 2 cut(s) 355, 1201
Bsh1236I CGCG 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 640
BshFI GGCC 6 cut(s) 429, 443, 452, 590, 639, 1252
BsiEI CGRYCG 1 cut(s) 640
BsiHKAI GWGCWC 1 cut(s) 1158
BsiHKCI CYCGRG 1 cut(s) 884
BsiSI CCGG 6 cut(s) 44, 426, 448, 830, 871, 1253
BslFI GGGAC 2 cut(s) 239, 1023
BslI CCNNNNNNNGG 3 cut(s) 137, 358, 940
BsmAI GTCTC 3 cut(s) 461, 974, 1370
BsmFI GGGAC 2 cut(s) 239, 1023
BsnI GGCC 6 cut(s) 429, 443, 452, 590, 639, 1252
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 884
Bsp1286I GDGCHC 2 cut(s) 696, 1158
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 4 cut(s) 633, 675, 849, 1466
Bsp19I CCATGG 1 cut(s) 136
BspANI GGCC 6 cut(s) 429, 443, 452, 590, 639, 1252
BspCNI CTCAG 2 cut(s) 769, 1372
BspFNI CGCG 1 cut(s) 551
BspPI GGATC 2 cut(s) 844, 1474
BspTI CTTAAG 1 cut(s) 1490
BspTNI GGTCTC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 1242
BsrFI RCCGGY 1 cut(s) 43
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 3 cut(s) 97, 325, 1321
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 4 cut(s) 136, 158, 315, 939
BssMI GATC 4 cut(s) 633, 675, 849, 1466
BssNI GRCGYC 3 cut(s) 85, 285, 333
BssT1I CCWWGG 2 cut(s) 136, 315
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 3 cut(s) 610, 940, 1401
Bst6I CTCTTC 3 cut(s) 25, 243, 1443
BstACI GRCGYC 3 cut(s) 85, 285, 333
BstAFI CTTAAG 1 cut(s) 1490
BstAUI TGTACA 1 cut(s) 603
BstBAI YACGTR 2 cut(s) 541, 1053
BstC8I GCNNGC 3 cut(s) 41, 549, 1318
BstDEI CTNAG 2 cut(s) 756, 1359
BstDSI CCRYGG 3 cut(s) 136, 158, 939
BstF5I GGATG 7 cut(s) 103, 348, 399, 405, 730, 1185, 1231
BstFNI CGCG 1 cut(s) 551
BstHHI GCGC 1 cut(s) 877
BstKTI GATC 4 cut(s) 636, 678, 852, 1469
BstMAI GTCTC 3 cut(s) 461, 974, 1370
BstMBI GATC 4 cut(s) 633, 675, 849, 1466
BstMCI CGRYCG 1 cut(s) 640
BstMWI GCNNNNNNNGC 3 cut(s) 435, 449, 901
BstNI CCWGG 1 cut(s) 440
BstPAI GACNNNNGTC 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 438, 447, 870
BstSFI CTRYAG 1 cut(s) 1384
BstSLI GKGCMC 1 cut(s) 1158
BstSNI TACGTA 2 cut(s) 541, 1053
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 2 cut(s) 355, 1201
BstZI CGGCCG 1 cut(s) 637
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 6 cut(s) 429, 443, 452, 590, 639, 1252
BtgI CCRYGG 3 cut(s) 136, 158, 939
BtgZI GCGATG 1 cut(s) 403
BtrI CACGTC 1 cut(s) 835
BtsCI GGATG 7 cut(s) 103, 348, 399, 405, 730, 1185, 1231
BtsIMutI CAGTG 2 cut(s) 606, 788
Cac8I GCNNGC 3 cut(s) 41, 549, 1318
CfoI GCGC 1 cut(s) 877
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 5 cut(s) 319, 361, 450, 774, 1250
CseI GACGC 2 cut(s) 93, 293
Csp6I GTAC 4 cut(s) 328, 604, 949, 1075
CviAII CATG 5 cut(s) 137, 578, 860, 1231, 1283
CviQI GTAC 4 cut(s) 328, 604, 949, 1075
DdeI CTNAG 2 cut(s) 756, 1359
DpnI GATC 4 cut(s) 635, 677, 851, 1468
DpnII GATC 4 cut(s) 633, 675, 849, 1466
DraIII CACNNNGTG 1 cut(s) 1151
EaeI YGGCCR 2 cut(s) 588, 637
EagI CGGCCG 1 cut(s) 637
Eam1104I CTCTTC 3 cut(s) 25, 243, 1443
EarI CTCTTC 3 cut(s) 25, 243, 1443
EciI GGCGGA 4 cut(s) 192, 311, 398, 668
EclXI CGGCCG 1 cut(s) 637
Eco105I TACGTA 2 cut(s) 541, 1053
Eco130I CCWWGG 2 cut(s) 136, 315
Eco24I GRGCYC 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 319, 361, 774
Eco52I CGGCCG 1 cut(s) 637
Eco57I CTGAAG 3 cut(s) 576, 812, 939
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 884
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 136, 315
EcoT38I GRGCYC 1 cut(s) 696
ErhI CCWWGG 2 cut(s) 136, 315
FaeI CATG 5 cut(s) 140, 581, 863, 1234, 1286
FaqI GGGAC 2 cut(s) 239, 1023
FatI CATG 5 cut(s) 136, 577, 859, 1230, 1282
FauI CCCGC 5 cut(s) 276, 443, 491, 974, 1235
Fnu4HI GCNGC 6 cut(s) 344, 387, 412, 552, 1047, 1190
FokI GGATG 7 cut(s) 90, 335, 386, 392, 737, 1192, 1238
FriOI GRGCYC 1 cut(s) 696
Fsp4HI GCNGC 6 cut(s) 344, 387, 412, 552, 1047, 1190
FspBI CTAG 1 cut(s) 840
GlaI GCGC 1 cut(s) 876
GluI GCNGC 6 cut(s) 344, 387, 412, 552, 1047, 1190
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 6 cut(s) 429, 443, 452, 590, 639, 1252
HapII CCGG 6 cut(s) 44, 426, 448, 830, 871, 1253
HgaI GACGC 2 cut(s) 93, 293
HhaI GCGC 1 cut(s) 877
Hin1I GRCGYC 3 cut(s) 85, 285, 333
Hin1II CATG 5 cut(s) 140, 581, 863, 1234, 1286
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HinfI GANTC 5 cut(s) 141, 1091, 1195, 1262, 1451
HpaII CCGG 6 cut(s) 44, 426, 448, 830, 871, 1253
HphI GGTGA 5 cut(s) 268, 367, 424, 1025, 1478
Hpy166II GTNNAC 4 cut(s) 606, 619, 951, 1156
Hpy188I TCNGA 6 cut(s) 283, 1096, 1119, 1362, 1405, 1471
Hpy188III TCNNGA 5 cut(s) 466, 478, 664, 722, 758
Hpy8I GTNNAC 4 cut(s) 606, 619, 951, 1156
Hpy99I CGWCG 7 cut(s) 87, 287, 314, 335, 338, 461, 949
HpyCH4III ACNGT 3 cut(s) 610, 940, 1401
HpyCH4IV ACGT 5 cut(s) 333, 540, 834, 947, 1052
HpyCH4V TGCA 5 cut(s) 751, 788, 1145, 1156, 1189
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 449, 901
HpyF3I CTNAG 2 cut(s) 756, 1359
HpySE526I ACGT 5 cut(s) 333, 540, 834, 947, 1052
Hsp92I GRCGYC 3 cut(s) 85, 285, 333
Hsp92II CATG 5 cut(s) 140, 581, 863, 1234, 1286
HspAI GCGC 1 cut(s) 875
Kzo9I GATC 4 cut(s) 633, 675, 849, 1466
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 2 cut(s) 355, 1201
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 840
MaeII ACGT 5 cut(s) 333, 540, 834, 947, 1052
MaeIII GTNAC 4 cut(s) 703, 835, 1366, 1456
MalI GATC 4 cut(s) 635, 677, 851, 1468
MbiI CCGCTC 1 cut(s) 1242
MboI GATC 4 cut(s) 633, 675, 849, 1466
MhlI GDGCHC 2 cut(s) 696, 1158
MluCI AATT 3 cut(s) 215, 1057, 1480
MlyI GAGTC 3 cut(s) 150, 1085, 1460
MmeI TCCRAC 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 797
MseI TTAA 2 cut(s) 1394, 1491
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 1192
MspCI CTTAAG 1 cut(s) 1490
MspI CCGG 6 cut(s) 44, 426, 448, 830, 871, 1253
MspR9I CCNGG 3 cut(s) 440, 449, 872
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 3 cut(s) 435, 449, 901
NciI CCSGG 2 cut(s) 449, 872
NcoI CCATGG 1 cut(s) 136
NdeII GATC 4 cut(s) 633, 675, 849, 1466
NlaIII CATG 5 cut(s) 140, 581, 863, 1234, 1286
NmuCI GTSAC 1 cut(s) 835
PcsI WCGNNNNNNNCGW 3 cut(s) 462, 471, 642
PdmI GAANNNNTTC 1 cut(s) 797
PfeI GAWTC 2 cut(s) 1195, 1262
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 6 cut(s) 345, 388, 413, 553, 1048, 1191
PleI GAGTC 3 cut(s) 149, 1085, 1459
PpsI GAGTC 3 cut(s) 149, 1085, 1459
Ppu21I YACGTR 2 cut(s) 541, 1053
PshAI GACNNNNGTC 1 cut(s) 473
PsiI TTATAA 1 cut(s) 1164
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspPI GGNCC 5 cut(s) 319, 361, 450, 774, 1250
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 1192
RsaI GTAC 4 cut(s) 329, 605, 950, 1076
RsaNI GTAC 4 cut(s) 328, 604, 949, 1075
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 2 cut(s) 1394, 1491
SatI GCNGC 6 cut(s) 344, 387, 412, 552, 1047, 1190
Sau3AI GATC 4 cut(s) 633, 675, 849, 1466
Sau96I GGNCC 5 cut(s) 319, 361, 450, 774, 1250
SchI GAGTC 3 cut(s) 150, 1085, 1460
ScrFI CCNGG 3 cut(s) 440, 449, 872
SduI GDGCHC 2 cut(s) 696, 1158
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 1384
SfiI GGCCNNNNNGGCC 1 cut(s) 449
SinI GGWCC 3 cut(s) 319, 361, 774
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 5 cut(s) 207, 664, 905, 989, 1490
SmoI CTYRAG 5 cut(s) 207, 664, 905, 989, 1490
SnaBI TACGTA 2 cut(s) 541, 1053
SpeI ACTAGT 1 cut(s) 839
Sse9I AATT 3 cut(s) 215, 1057, 1480
SspI AATATT 1 cut(s) 1168
SspMI CTAG 1 cut(s) 840
StyD4I CCNGG 3 cut(s) 438, 447, 870
StyI CCWWGG 2 cut(s) 136, 315
TaaI ACNGT 3 cut(s) 610, 940, 1401
TaiI ACGT 5 cut(s) 336, 543, 837, 950, 1055
TasI AATT 3 cut(s) 215, 1057, 1480
TatI WGTACW 2 cut(s) 603, 1074
TauI GCSGC 4 cut(s) 389, 414, 554, 1049
TfiI GAWTC 2 cut(s) 1195, 1262
Tru1I TTAA 2 cut(s) 1394, 1491
Tru9I TTAA 2 cut(s) 1394, 1491
TscAI CASTG 2 cut(s) 613, 795
TseFI GTSAC 1 cut(s) 835
TseI GCWGC 2 cut(s) 343, 1189
Tsp45I GTSAC 1 cut(s) 835
TspDTI ATGAA 7 cut(s) 819, 848, 1139, 1247, 1254, 1271, 1461
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 2 cut(s) 613, 795
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 1490
VneI GTGCAC 1 cut(s) 1154
VpaK11BI GGWCC 3 cut(s) 319, 361, 774
XmnI GAANNNNTTC 1 cut(s) 797
XspI CTAG 1 cut(s) 840
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.