pycom17g23800

Pentatricopeptide repeat-containing protein At2g13420, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006370
Physical Location & Seq
Forward (+)
22089474 .. 22090310
837 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGATTGAGCGCGGTATTGAGCCCAATGTCGTTACATATAATGTCTTCTTGAATGGTATTTGTAGGCGTGCGAGTTTGCACCCTCAGGAAAGGTTTGAGAAGACGATAAGGAACGCAGAGAAGGTGTTTGAGGAAATGCGGAAGAGAGGGATAGAGCCCGATGTCACTAGTTTTTCGATTGTGCTTCATGTTTATAGCCGTGCTCATAAGCCCGAGTTGTCGCTTGATAAGCTCAAACTGATGAGGGAGAGAGGGATATTCCCCACAGTGGAAACTTACACTTCGGTTGTAAAGTGTCTTTGCTCGTGCGGGAGACTTGAGGATGCAGAGGAGCTGCTTGGTGAAATGGTGAGAAGTGGGGTTAGTCCATGTGCAGCTACGTATAATTGTTTCTTCAAAGAGTACAGGGGGAGAAAGAACGGGGAAGGTGCTCTGAAGTTGTACAGGAAAATGAAGGAGGATGGTTTGTGTGTGCCCAGTATGCACACCTATAACATATTGGTGGGAATGCTTTTGGAATTGAATCGAATGGAGATTGTGAAAGAAATATGGAATGATATGAAAGAGAGTGGAGTGGGACCGGATTTGGATTCGTATACCATGTTGATTCATGGGTTATGTGCAAAACAGAAGTGGAGGGAGGCTTGTCAGTTCTTTGTAGAGATGATAGAGAAGGGTCTTCTTCCTCAGAAGATTACTTTTGAGACACTCTACAAAGGGTTGATACAATCTGATATGTTGAGAACTTGGCGAAGATTGAAGAAGAAACTCGATGAAGAGTCTATTTCGTTTGGGTCAGAGTTCCAAAAGTATCACCTTAAGCCATTTAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

279

Amino Acids

32.44

Weight (kDa)

8.95

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 6 - 128 4.9e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 36 - 65 2.9e-07 PPR repeat family
PPR_3 PF13812 41 - 98 2.7e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 59 - 101 6.2e-06 PPR repeat family
TPR_24 PF23276 61 - 178 1.1e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 84 - 117 7.9e-11 PPR repeat
PPR_2 PF13041 92 - 134 4.4e-09 PPR repeat family
PPR PF01535 92 - 121 3.7e-08 PPR repeat
PPR_3 PF13812 112 - 171 2.3e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 123 - 171 1.2e-08 PPR repeat family
PPR_2 PF13041 159 - 207 3e-12 PPR repeat family
PPR_1 PF12854 191 - 222 2.6e-07 PPR repeat
PPR_2 PF13041 198 - 241 1.2e-09 PPR repeat family
PPR PF01535 198 - 227 6.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 596
AccII CGCG 1 cut(s) 12
AciI CCGC 3 cut(s) 12, 139, 309
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 2 cut(s) 404, 443
AfiI CCNNNNNNNGG 1 cut(s) 268
AflII CTTAAG 1 cut(s) 818
AgsI TTSAA 4 cut(s) 52, 397, 523, 760
AhlI ACTAGT 1 cut(s) 167
AluBI AGCT 3 cut(s) 232, 334, 377
AluI AGCT 3 cut(s) 232, 334, 377
Alw21I GWGCWC 2 cut(s) 205, 433
Alw26I GTCTC 2 cut(s) 307, 698
Ama87I CYCGRG 1 cut(s) 212
ApeKI GCWGC 2 cut(s) 334, 374
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 3 cut(s) 353, 361, 806
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 477
BanII GRGCYC 2 cut(s) 24, 159
BauI CACGAG 1 cut(s) 304
BbsI GAAGAC 3 cut(s) 37, 107, 671
Bbv12I GWGCWC 2 cut(s) 205, 433
BbvI GCAGC 2 cut(s) 321, 386
BccI CCATC 1 cut(s) 455
BceAI ACGGC 1 cut(s) 183
BcoDI GTCTC 2 cut(s) 307, 698
BcuI ACTAGT 1 cut(s) 167
BfaI CTAG 1 cut(s) 168
BfrI CTTAAG 1 cut(s) 818
BisI GCNGC 2 cut(s) 335, 375
BlsI GCNGC 2 cut(s) 336, 376
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 579
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 313
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 3 cut(s) 37, 107, 671
BpuEI CTTGAG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 381
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 477
Bse21I CCTNAGG 1 cut(s) 84
BseGI GGATG 2 cut(s) 328, 466
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 2 cut(s) 98, 701
BseNI ACTGG 1 cut(s) 477
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 2 cut(s) 321, 386
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 205, 433
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 307, 698
BsmFI GGGAC 1 cut(s) 591
BsmI GAATGC 1 cut(s) 513
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 5 cut(s) 24, 159, 205, 433, 477
Bsp1407I TGTACA 1 cut(s) 441
BspACI CCGC 3 cut(s) 12, 139, 309
BspCNI CTCAG 2 cut(s) 97, 700
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 579
BspTI CTTAAG 1 cut(s) 818
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 1 cut(s) 477
BssNAI GTATAC 1 cut(s) 597
BssSI CACGAG 1 cut(s) 304
Bst1107I GTATAC 1 cut(s) 597
Bst2BI CACGAG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 137, 771
BstAFI CTTAAG 1 cut(s) 818
BstAUI TGTACA 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 84, 687
BstF5I GGATG 2 cut(s) 328, 466
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 12
BstMAI GTCTC 2 cut(s) 307, 698
BstMWI GCNNNNNNNGC 2 cut(s) 229, 481
BstSLI GKGCMC 1 cut(s) 477
BstSNI TACGTA 1 cut(s) 381
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 321, 386
BstV2I GAAGAC 3 cut(s) 37, 107, 671
BstZ17I GTATAC 1 cut(s) 597
Bsu36I CCTNAGG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 328, 466
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 12
Cfr13I GGNCC 1 cut(s) 578
Csp6I GTAC 2 cut(s) 403, 442
CviAII CATG 4 cut(s) 188, 369, 601, 611
CviJI RGCY 9 cut(s) 22, 157, 198, 211, 232, 334, 377, 644, 823
CviKI_1 RGCY 9 cut(s) 22, 157, 198, 211, 232, 334, 377, 644, 823
CviQI GTAC 2 cut(s) 403, 442
DdeI CTNAG 2 cut(s) 84, 687
Eam1104I CTCTTC 2 cut(s) 137, 771
EarI CTCTTC 2 cut(s) 137, 771
Eco105I TACGTA 1 cut(s) 381
Eco24I GRGCYC 2 cut(s) 24, 159
Eco47I GGWCC 1 cut(s) 578
Eco57I CTGAAG 1 cut(s) 455
Eco81I CCTNAGG 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 212
EcoT38I GRGCYC 2 cut(s) 24, 159
FaeI CATG 4 cut(s) 191, 372, 604, 614
FaqI GGGAC 1 cut(s) 591
FatI CATG 4 cut(s) 187, 368, 600, 610
FauI CCCGC 1 cut(s) 302
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 2 cut(s) 335, 375
FokI GGATG 2 cut(s) 335, 473
FriOI GRGCYC 2 cut(s) 24, 159
Fsp4HI GCNGC 2 cut(s) 335, 375
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 11
GluI GCNGC 2 cut(s) 335, 375
HapII CCGG 1 cut(s) 581
HhaI GCGC 1 cut(s) 12
Hin1II CATG 4 cut(s) 191, 372, 604, 614
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HinfI GANTC 4 cut(s) 523, 590, 607, 779
HpaII CCGG 1 cut(s) 581
HphI GGTGA 3 cut(s) 353, 361, 806
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 4 cut(s) 435, 690, 733, 799
Hpy188III TCNNGA 2 cut(s) 49, 86
Hpy8I GTNNAC 1 cut(s) 597
HpyAV CCTTC 4 cut(s) 115, 419, 448, 667
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 79, 326, 374, 484, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 481
HpyF3I CTNAG 2 cut(s) 84, 687
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 4 cut(s) 191, 372, 604, 614
HspAI GCGC 1 cut(s) 10
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 5 cut(s) 71, 391, 430, 490, 594
Lsp1109I GCAGC 2 cut(s) 321, 386
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 31, 163
MhlI GDGCHC 5 cut(s) 24, 159, 205, 433, 477
MluCI AATT 2 cut(s) 385, 518
MlyI GAGTC 1 cut(s) 788
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 1 cut(s) 581
Mva1269I GAATGC 1 cut(s) 513
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 229, 481
NlaIII CATG 4 cut(s) 191, 372, 604, 614
NlaIV GGNNCC 1 cut(s) 579
NmuCI GTSAC 1 cut(s) 163
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 523, 590, 607
PkrI GCNGC 2 cut(s) 336, 376
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
Ppu21I YACGTR 1 cut(s) 381
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 1 cut(s) 578
RsaI GTAC 2 cut(s) 404, 443
RsaNI GTAC 2 cut(s) 403, 442
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 2 cut(s) 335, 375
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 1 cut(s) 788
SduI GDGCHC 5 cut(s) 24, 159, 205, 433, 477
SetI ASST 9 cut(s) 95, 126, 234, 336, 379, 383, 430, 491, 819
SfaNI GCATC 1 cut(s) 313
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 2 cut(s) 317, 818
SmoI CTYRAG 2 cut(s) 317, 818
SnaBI TACGTA 1 cut(s) 381
SpeI ACTAGT 1 cut(s) 167
Sse9I AATT 2 cut(s) 385, 518
SsiI CCGC 3 cut(s) 12, 139, 309
SspMI CTAG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 383
TaqI TCGA 3 cut(s) 176, 526, 771
TasI AATT 2 cut(s) 385, 518
TatI WGTACW 2 cut(s) 402, 441
TfiI GAWTC 3 cut(s) 523, 590, 607
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 334, 374
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 5 cut(s) 176, 467, 575, 599, 789
TspRI CASTG 1 cut(s) 273
Vha464I CTTAAG 1 cut(s) 818
VpaK11BI GGWCC 1 cut(s) 578
XmiI GTMKAC 1 cut(s) 596
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.