pycom17g26100

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004429
Physical Location & Seq
Reverse (-)
24395857 .. 24404556
8700 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1893 bp
ATGATCGATAATGATTACAATCTTGCAAGAAAGCTTGGTCTTCCAATACTTAATGTCATGAACAAGGATGGAACGCTAAACAAGGTTTCCGGACTGTACTGTGGCCTTGATCGGTTTGAGGCCCGGAAGAAACTTTGGGCAGATCTGGAGGAGACTGGTTTAGCTGTGAAGAAAGAGCCGCATACTTTACGAGTTCCCAGATCTCAGCGTGGTGGAGAAGTTATAGAGCCACTAGTAAGCAAGCAGTGGTTTGTAACCATGGAGCCCTTAGCTGAGAAAGCCCTTAGTGCTGTTGAAAAAGGAGACCTGAAAATTATACCTGAAAGATTTGAAAAGATTTACAATCACTGGCTATCAAATATTAAGGATTGGTGCATAAGCAGACAACTGTGGTGGGGTCATCGAATACCAGTTTGGTACATTGTGGGTAAAGATTGTGAAGAAGAATATATAGTTGCAAGGAGTGAGGATGAAGCTCTTGGGAAAGCTCAAGAAAAGTATGGAAAAGATGCAAAAATATATCAGGATCCGGATGTTCTTGACACATGGTTTTCGAGTGCACTGTGGCCTTTCAGTACTCTTGGATGGCCAGATGAATCAGTTGAGGATTTTAAGCGGTTTTATCCTACAACAATGCTTGAAACTGGGCATGACATATTGTTCTTTTGGGTTGCGAGGATGGTCATGATGGGAATTGAGTTCACAGGGACAGTACCATTTAAATACATTTATCTACACGGTCTCATCAGGGATTCACAAGGTCGAAAGATGTCGAAAACATTGGGGAATGTAATAGATCCTTTAGATACAATCAAGGAATATGGCACAGATGCTTTAAGATTTACAATTGCTCTAGGGACTGCTGGCCAGGACCTTAATTTATCCACTGAGAGGTTGACATCCAACAAGGCCTTCACTAATAAACTATGGAATGCTGGAAAATTTGTATTGCACAATTTACCCAGTCAAAACGATGCATCAATTTGGGAAAATATACTTTCTTTTAAGTTTGACAAAGTGGAGTTCCTAGACAAGCTACCTTTACCAGAATGTTGGGTGATTTCAAAACTTCATTTGCTTATTGACACAGTCACCACAAGCTATGACAAATTTTTCTTTGGAGATGTAGGAAGAGAGACATATGATTTCTTTTGGGGAGATTTTGCTGATTGGTACATCGAGGCCAGTAAAGCTCGTCTTTACCACTCTGGTGGTGGTTCAGTTGCTTCTGTCACACAGTCAGTTCTATTGTATGTTTTTGAAAACATACTTAAACTACTACACCCCTTCATGCCATTTGTGACCGAGGAACTCTGGCAGGCACTCCCCTACCGAAAAGAAGCTCTTATTGTATCTCCGTGGCCACTGACTTCACTTCCAAGGAAGAGCAATTCGATTAAAAATTTTGAAAATTTACAGGCTTTGACTAGAGCTATCAGAAATGCTCGAGCAGAATATTCTGTTGAGCCTGTAAAGCGTATATCAGCATCTATAGTTGCAAATGAAGAAGTCACTGAATACATACAGAAGGAAAAGGAAGTTTTAGCTCTTCTCTCCAGGCTAGATTTACAAAACATCCATTTTACCGATTCTCCTCCAGGGAATGCGGATCAATCAGTCCATCTTGTAGCTGGTGAAGGGCTAGAGGCATATCTCCCTCTTGCTGACATGATTGATATTACTGCTGAAATCCAACGCCTATCTAAGAGACTTTCCAAGATGCAGACAGAATATGATGGACTTAAAGCTCGGCTCAGCTCTCCTAAATTTGTGGAGAAGGCTCCTGAAGATATTGTCCGTGGAGTTCAAGAAAAGGCAGCAGAAACAGAAGAGAAGATTACTCTCACCAAAAACCGATTAGCATTGCTCGAATCAAGTGTTCTGGTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

631

Amino Acids

72.06

Weight (kDa)

5.68

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 57 - 137 4.2e-06 tRNA synthetases class I (M)
tRNA-synt_1 PF00133 72 - 295 4.9e-80 tRNA synthetases class I (I, L, M and V)
Anticodon_1 PF08264 352 - 494 1.1e-38 Anticodon-binding domain of tRNA ligase
Val_tRNA-synt_C PF10458 559 - 623 4e-14 Valyl tRNA synthetase tRNA binding arm
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 89, 529
AciI CCGC 3 cut(s) 179, 616, 1607
AclWI GGATC 4 cut(s) 521, 534, 791, 1617
AcoI YGGCCR 3 cut(s) 587, 865, 1361
AcsI RAATTY 5 cut(s) 941, 1109, 1402, 1411, 1766
AcuI CTGAAG 1 cut(s) 1806
AfaI GTAC 5 cut(s) 98, 419, 577, 714, 1175
AfiI CCNNNNNNNGG 1 cut(s) 891
AgsI TTSAA 7 cut(s) 296, 332, 641, 1065, 1262, 1409, 1808
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 3 cut(s) 867, 1556, 1597
AjuI GAANNNNNNNTTGG 4 cut(s) 397, 429, 896, 928
AleI CACNNNNGTG 1 cut(s) 1209
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 5 cut(s) 146, 297, 746, 1130, 1702
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 4 cut(s) 521, 534, 791, 1617
Ama87I CYCGRG 1 cut(s) 1446
Aor13HI TCCGGA 2 cut(s) 89, 529
AoxI GGCC 8 cut(s) 103, 120, 566, 587, 865, 909, 1182, 1361
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 1 cut(s) 1817
ApoI RAATTY 5 cut(s) 941, 1109, 1402, 1411, 1766
AspS9I GGNCC 2 cut(s) 121, 871
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 4 cut(s) 1069, 1084, 1646, 1837
AvaI CYCGRG 1 cut(s) 1446
AvaII GGWCC 1 cut(s) 871
BaeGI GKGCMC 1 cut(s) 562
BalI TGGCCA 3 cut(s) 589, 867, 1363
BamHI GGATCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 1 cut(s) 1829
BccI CCATC 6 cut(s) 62, 579, 673, 682, 1629, 1730
BciT130I CCWGG 3 cut(s) 869, 1558, 1599
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 5 cut(s) 146, 297, 746, 1130, 1702
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 6 cut(s) 233, 854, 1028, 1428, 1562, 1643
BfmI CTRYAG 1 cut(s) 1491
BglII AGATCT 2 cut(s) 142, 200
BisI GCNGC 2 cut(s) 179, 1818
BlpI GCTNAGC 1 cut(s) 1754
BlsI GCNGC 2 cut(s) 180, 1819
BmcAI AGTACT 1 cut(s) 577
Bme1390I CCNGG 4 cut(s) 124, 869, 1558, 1599
Bme18I GGWCC 1 cut(s) 871
BmeT110I CYCGRG 1 cut(s) 1446
BmgT120I GGNCC 2 cut(s) 121, 871
BmiI GGNNCC 3 cut(s) 264, 528, 1782
BmrFI CCNGG 4 cut(s) 124, 869, 1558, 1599
BmrI ACTGGG 2 cut(s) 654, 957
BmsI GCATC 6 cut(s) 499, 820, 964, 986, 1496, 1710
BmuI ACTGGG 2 cut(s) 654, 957
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 3 cut(s) 167, 1540, 1581
Bpu10I CCTNAGC 1 cut(s) 268
Bpu1102I GCTNAGC 1 cut(s) 1754
BpuEI CTTGAG 1 cut(s) 474
BpuMI CCSGG 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 297, 746
BsaJI CCNNGG 6 cut(s) 258, 1305, 1358, 1379, 1598, 1798
BsaWI WCCGGW 2 cut(s) 89, 529
BsaXI ACNNNNNCTCC 2 cut(s) 1576, 1606
Bsc4I CCNNNNNNNGG 1 cut(s) 891
Bse1I ACTGG 6 cut(s) 160, 353, 410, 649, 963, 1185
Bse3DI GCAATG 1 cut(s) 1862
Bse8I GATNNNNATC 1 cut(s) 18
BseAI TCCGGA 2 cut(s) 89, 529
BseBI CCWGG 3 cut(s) 869, 1558, 1599
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 6 cut(s) 258, 1305, 1358, 1379, 1598, 1798
BseGI GGATG 7 cut(s) 73, 475, 538, 590, 684, 899, 1575
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 891
BseMI GCAATG 1 cut(s) 1862
BseMII CTCAG 4 cut(s) 218, 264, 879, 1768
BseNI ACTGG 6 cut(s) 160, 353, 410, 649, 963, 1185
BseRI GAGGAG 2 cut(s) 164, 1584
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 1 cut(s) 1829
BshFI GGCC 8 cut(s) 105, 122, 568, 589, 867, 911, 1184, 1363
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 1446
BsiSI CCGG 3 cut(s) 90, 124, 530
BslFI GGGAC 2 cut(s) 721, 871
BslI CCNNNNNNNGG 1 cut(s) 891
BsmAI GTCTC 5 cut(s) 146, 297, 746, 1130, 1702
BsmFI GGGAC 2 cut(s) 721, 871
BsmI GAATGC 2 cut(s) 937, 1609
BsnI GGCC 8 cut(s) 105, 122, 568, 589, 867, 911, 1184, 1363
Bso31I GGTCTC 2 cut(s) 297, 746
BsoBI CYCGRG 1 cut(s) 1446
Bsp1286I GDGCHC 2 cut(s) 267, 562
Bsp13I TCCGGA 2 cut(s) 89, 529
Bsp143I GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
Bsp1720I GCTNAGC 1 cut(s) 1754
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 3 cut(s) 179, 616, 1607
BspANI GGCC 8 cut(s) 105, 122, 568, 589, 867, 911, 1184, 1363
BspCNI CTCAG 4 cut(s) 217, 265, 880, 1767
BspDI ATCGAT 1 cut(s) 6
BspEI TCCGGA 2 cut(s) 89, 529
BspHI TCATGA 2 cut(s) 57, 684
BspLI GGNNCC 3 cut(s) 264, 528, 1782
BspPI GGATC 4 cut(s) 521, 534, 791, 1617
BspQI GCTCTTC 2 cut(s) 1379, 1554
BspTNI GGTCTC 2 cut(s) 297, 746
BsrDI GCAATG 1 cut(s) 1862
BsrI ACTGG 6 cut(s) 160, 353, 410, 649, 963, 1185
BssECI CCNNGG 6 cut(s) 258, 1305, 1358, 1379, 1598, 1798
BssMI GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
BssT1I CCWWGG 2 cut(s) 258, 1379
Bst2UI CCWGG 3 cut(s) 869, 1558, 1599
Bst4CI ACNGT 8 cut(s) 96, 101, 390, 564, 712, 740, 1090, 1239
Bst6I CTCTTC 4 cut(s) 1126, 1379, 1554, 1824
BstC8I GCNNGC 3 cut(s) 242, 865, 1320
BstDEI CTNAG 7 cut(s) 204, 268, 273, 284, 888, 1704, 1754
BstDSI CCRYGG 3 cut(s) 258, 1358, 1798
BstF5I GGATG 7 cut(s) 73, 475, 538, 590, 684, 899, 1575
BstKTI GATC 7 cut(s) 6, 112, 145, 203, 529, 799, 1612
BstMAI GTCTC 5 cut(s) 146, 297, 746, 1130, 1702
BstMBI GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
BstMWI GCNNNNNNNGC 4 cut(s) 278, 287, 1190, 1474
BstNI CCWGG 3 cut(s) 869, 1558, 1599
BstSCI CCNGG 4 cut(s) 122, 867, 1556, 1597
BstSFI CTRYAG 1 cut(s) 1491
BstSLI GKGCMC 1 cut(s) 562
BstV1I GCAGC 1 cut(s) 1829
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 4 cut(s) 142, 200, 526, 796
BstXI CCANNNNNNTGG 2 cut(s) 1053, 1211
BstYI RGATCY 4 cut(s) 142, 200, 526, 796
Bsu15I ATCGAT 1 cut(s) 6
BsuRI GGCC 8 cut(s) 105, 122, 568, 589, 867, 911, 1184, 1363
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 3 cut(s) 258, 1358, 1798
BtsCI GGATG 7 cut(s) 73, 475, 538, 590, 684, 899, 1575
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 6 cut(s) 251, 346, 560, 885, 1364, 1512
Cac8I GCNNGC 3 cut(s) 242, 865, 1320
CciI TCATGA 2 cut(s) 57, 684
Cfr13I GGNCC 2 cut(s) 121, 871
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 5 cut(s) 97, 418, 576, 713, 1174
CspCI CAANNNNNGTGG 2 cut(s) 374, 409
CviAII CATG 7 cut(s) 58, 259, 546, 650, 685, 1291, 1669
CviQI GTAC 5 cut(s) 97, 418, 576, 713, 1174
DdeI CTNAG 7 cut(s) 204, 268, 273, 284, 888, 1704, 1754
DpnI GATC 7 cut(s) 5, 111, 144, 202, 528, 798, 1611
DpnII GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
DraI TTTAAA 1 cut(s) 721
EaeI YGGCCR 3 cut(s) 587, 865, 1361
Eam1104I CTCTTC 4 cut(s) 1126, 1379, 1554, 1824
EarI CTCTTC 4 cut(s) 1126, 1379, 1554, 1824
Eco130I CCWWGG 2 cut(s) 258, 1379
Eco147I AGGCCT 1 cut(s) 911
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 297, 746
Eco47I GGWCC 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 1806
Eco88I CYCGRG 1 cut(s) 1446
EcoO109I RGGNCCY 1 cut(s) 871
EcoRII CCWGG 3 cut(s) 867, 1556, 1597
EcoT14I CCWWGG 2 cut(s) 258, 1379
EcoT22I ATGCAT 1 cut(s) 979
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 258, 1379
FaeI CATG 7 cut(s) 61, 262, 549, 653, 688, 1294, 1672
FalI AAGNNNNNCTT 4 cut(s) 1182, 1214, 1329, 1361
FaqI GGGAC 2 cut(s) 721, 871
FatI CATG 7 cut(s) 57, 258, 545, 649, 684, 1290, 1668
FauNDI CATATG 1 cut(s) 1141
Fnu4HI GCNGC 2 cut(s) 179, 1818
FokI GGATG 7 cut(s) 80, 482, 545, 597, 691, 886, 1562
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 2 cut(s) 179, 1818
FspBI CTAG 6 cut(s) 233, 854, 1028, 1428, 1562, 1643
GluI GCNGC 2 cut(s) 179, 1818
GsuI CTGGAG 3 cut(s) 167, 1540, 1581
HaeIII GGCC 8 cut(s) 105, 122, 568, 589, 867, 911, 1184, 1363
HapII CCGG 3 cut(s) 90, 124, 530
Hin1II CATG 7 cut(s) 61, 262, 549, 653, 688, 1294, 1672
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 4 cut(s) 596, 752, 1589, 1871
HpaII CCGG 3 cut(s) 90, 124, 530
HphI GGTGA 4 cut(s) 1069, 1084, 1646, 1837
Hpy166II GTNNAC 3 cut(s) 560, 702, 897
Hpy188I TCNGA 1 cut(s) 1439
Hpy8I GTNNAC 3 cut(s) 560, 702, 897
HpyAV CCTTC 5 cut(s) 922, 1297, 1522, 1631, 1771
HpyCH4III ACNGT 8 cut(s) 96, 101, 390, 564, 712, 740, 1090, 1239
HpyCH4V TGCA 9 cut(s) 26, 375, 458, 512, 560, 952, 977, 1499, 1723
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 287, 1190, 1474
HpyF3I CTNAG 7 cut(s) 204, 268, 273, 284, 888, 1704, 1754
Hsp92II CATG 7 cut(s) 61, 262, 549, 653, 688, 1294, 1672
Kpn2I TCCGGA 2 cut(s) 89, 529
Kzo9I GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
LguI GCTCTTC 2 cut(s) 1379, 1554
LmnI GCTCC 2 cut(s) 262, 1786
Lsp1109I GCAGC 1 cut(s) 1829
LweI GCATC 6 cut(s) 499, 820, 964, 986, 1496, 1710
MaeI CTAG 6 cut(s) 233, 854, 1028, 1428, 1562, 1643
MaeIII GTNAC 6 cut(s) 253, 1090, 1231, 1300, 1510, 1885
MalI GATC 7 cut(s) 5, 111, 144, 202, 528, 798, 1611
MboI GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 4 cut(s) 142, 200, 526, 796
MhlI GDGCHC 2 cut(s) 267, 562
MlsI TGGCCA 3 cut(s) 589, 867, 1363
MluNI TGGCCA 3 cut(s) 589, 867, 1363
MmeI TCCRAC 2 cut(s) 927, 1717
Mox20I TGGCCA 3 cut(s) 589, 867, 1363
Mph1103I ATGCAT 1 cut(s) 979
MroI TCCGGA 2 cut(s) 89, 529
MscI TGGCCA 3 cut(s) 589, 867, 1363
MslI CAYNNNNRTG 1 cut(s) 1209
Msp20I TGGCCA 3 cut(s) 589, 867, 1363
MspI CCGG 3 cut(s) 90, 124, 530
MspR9I CCNGG 4 cut(s) 124, 869, 1558, 1599
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 2 cut(s) 937, 1609
MvaI CCWGG 3 cut(s) 869, 1558, 1599
MwoI GCNNNNNNNGC 4 cut(s) 278, 287, 1190, 1474
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 1141
NdeII GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
NlaIII CATG 7 cut(s) 61, 262, 549, 653, 688, 1294, 1672
NlaIV GGNNCC 3 cut(s) 264, 528, 1782
NmeAIII GCCGAG 1 cut(s) 1729
NmuCI GTSAC 5 cut(s) 1090, 1231, 1300, 1510, 1885
NsiI ATGCAT 1 cut(s) 979
OliI CACNNNNGTG 1 cut(s) 1209
PaeR7I CTCGAG 1 cut(s) 1446
PagI TCATGA 2 cut(s) 57, 684
PceI AGGCCT 1 cut(s) 911
PciSI GCTCTTC 2 cut(s) 1379, 1554
PctI GAATGC 2 cut(s) 937, 1609
PfeI GAWTC 4 cut(s) 596, 752, 1589, 1871
PflFI GACNNNGTC 1 cut(s) 1088
PkrI GCNGC 2 cut(s) 180, 1819
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 3 cut(s) 867, 1556, 1597
PspGI CCWGG 3 cut(s) 867, 1556, 1597
PspN4I GGNNCC 3 cut(s) 264, 528, 1782
PspPI GGNCC 2 cut(s) 121, 871
PspPPI RGGWCCY 1 cut(s) 871
PspXI VCTCGAGB 1 cut(s) 1446
PsuI RGATCY 4 cut(s) 142, 200, 526, 796
PsyI GACNNNGTC 1 cut(s) 1088
RsaI GTAC 5 cut(s) 98, 419, 577, 714, 1175
RsaNI GTAC 5 cut(s) 97, 418, 576, 713, 1174
RseI CAYNNNNRTG 1 cut(s) 1209
SapI GCTCTTC 2 cut(s) 1379, 1554
SatI GCNGC 2 cut(s) 179, 1818
Sau3AI GATC 7 cut(s) 3, 109, 142, 200, 526, 796, 1609
Sau96I GGNCC 2 cut(s) 121, 871
ScaI AGTACT 1 cut(s) 577
ScrFI CCNGG 4 cut(s) 124, 869, 1558, 1599
SduI GDGCHC 2 cut(s) 267, 562
SfaNI GCATC 6 cut(s) 499, 820, 964, 986, 1496, 1710
SfcI CTRYAG 1 cut(s) 1491
Sfr274I CTCGAG 1 cut(s) 1446
SinI GGWCC 1 cut(s) 871
SlaI CTCGAG 1 cut(s) 1446
SmiI ATTTAAAT 1 cut(s) 721
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 2 cut(s) 489, 1446
SmoI CTYRAG 2 cut(s) 489, 1446
SpeI ACTAGT 1 cut(s) 232
SseBI AGGCCT 1 cut(s) 911
SsiI CCGC 3 cut(s) 179, 616, 1607
SspI AATATT 2 cut(s) 361, 1457
SspMI CTAG 6 cut(s) 233, 854, 1028, 1428, 1562, 1643
StuI AGGCCT 1 cut(s) 911
StyD4I CCNGG 4 cut(s) 122, 867, 1556, 1597
StyI CCWWGG 2 cut(s) 258, 1379
SwaI ATTTAAAT 1 cut(s) 721
TaaI ACNGT 8 cut(s) 96, 101, 390, 564, 712, 740, 1090, 1239
TaqI TCGA 9 cut(s) 6, 403, 554, 763, 773, 1179, 1394, 1447, 1869
TaqII GACCGA 1 cut(s) 1319
TatI WGTACW 2 cut(s) 96, 575
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 4 cut(s) 596, 752, 1589, 1871
TscAI CASTG 6 cut(s) 251, 353, 567, 892, 1371, 1519
TseFI GTSAC 5 cut(s) 1090, 1231, 1300, 1510, 1885
TseI GCWGC 1 cut(s) 1817
Tsp45I GTSAC 5 cut(s) 1090, 1231, 1300, 1510, 1885
TspDTI ATGAA 6 cut(s) 74, 486, 609, 1061, 1279, 1518
TspGWI ACGGA 2 cut(s) 1347, 1787
TspRI CASTG 6 cut(s) 251, 353, 567, 892, 1371, 1519
Tth111I GACNNNGTC 1 cut(s) 1088
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 871
XapI RAATTY 5 cut(s) 941, 1109, 1402, 1411, 1766
XcmI CCANNNNNNNNNTGG 2 cut(s) 1211, 1628
XhoI CTCGAG 1 cut(s) 1446
XspI CTAG 6 cut(s) 233, 854, 1028, 1428, 1562, 1643
ZrmI AGTACT 1 cut(s) 577
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.