pycom17g27640

Protein of unknown function (DUF724)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004216
Physical Location & Seq
Reverse (-)
25773808 .. 25776461
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1179 bp
ATGGCGGGCTCGGAGGGCAGAGAAGAAGAGCAGCAGGAGCTCCAATTGTTCAGCGTGGGCTCAGAAGTAGAAGTGAGGACCGACGAGGAAGGGTTCAAAGGCGCATTGTTCAGAGCCACCATTGTCACAAGCCCCACAAACTCCGCCTCAAAGAAGAGGAAAAGGGCGTTGGTCGAGTACAAGAACTTGGTCACGGAAGACGGGTCTAAGCAGCTCAAGGAGTACGTCGATTTGGAGCACCTGAGGCCCACGCCGCCGCAGCTCGCTGACCGGAATTTTGAGGAGGGAGATGTGGTGGATGCGGATTACAGAGATGGGTGGTGGACTGGGGTCGTAGGGAAGGTTGTCGAGAACAATTCCAAGTACACCGTCGTCTTCGACAACCCTCCGGATTTGATCGAATTTCAGAAAGAACGTCTCAGATTGCATCAAGATTGGGTTAATGGCGAATGGGTTCGGCCCAATAAGCAGGAAGTATTGGCTGCTTTAGATACTCCACAAGAATTAGAGCATGTGTTGCCTGCTCAAAAAGATTCTAACAATCTTGCAGTGGCTACTAAACTAGAAAATTTGAGTGCTGCAGAGGAGAATCCTGAATCAACCAATTCAGGGAAGAAAATGGAGCAGCCAAGTTATCCACGAAGCGTCAAGGACAAAAAGATGTTAGCCCGCAATGGTAGTGCTACAGACTCGCGTCCGGTTAAAAAGTTGAAGGACGACAAGGCAGCCGAGCCCATATTATCCATTACAGCACGTCAATTGAGGAAAACGCCTGACAATAAAGAAATGCCCCAGGAATTACCTCAACTGAGCACAGGAGTTAGGGGAACAAGACGCACACGGAAACCTGTTGTACGCCATCAGTTTATTAAAACAGAGAGCCTGCTGGGGAAAAATAATGTTAAGACTAAGCAAGAGAAGGACGGTGAAGTGAATAGCCAGTGGATTCATCCCGTAGCAAGTAAAGGAAGGCGCACAAAATCTCAAACTGGAAGTCGATTGATCTCAGAATCAGATAAGGAAGCGTCTGCAAATGTTTCTTTGGCTGGACAAAATATTCAGAATGAGGGTAATATTAAAGAAACTGAAGTGCCTCAAACTATCGGGTCAACAACTAAGGGCAAGGAAGTTTCACTGGCTGAAAAACCGGCTCACTACCTGATCAAGAGTTGCAAGTGA

Protein Analysis

393

Amino Acids

43.92

Weight (kDa)

8.61

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 20 - 86 2.6e-17 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 694
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 6 cut(s) 5, 144, 254, 257, 302, 670
AcsI RAATTY 3 cut(s) 274, 401, 568
AcuI CTGAAG 1 cut(s) 1107
AfaI GTAC 4 cut(s) 179, 224, 365, 855
AfiI CCNNNNNNNGG 1 cut(s) 609
AgsI TTSAA 2 cut(s) 97, 712
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 40, 214, 262
AluI AGCT 3 cut(s) 40, 214, 262
Alw21I GWGCWC 3 cut(s) 42, 240, 815
Alw26I GTCTC 1 cut(s) 422
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 2 cut(s) 245, 458
ApeKI GCWGC 7 cut(s) 31, 211, 259, 482, 578, 625, 725
ApoI RAATTY 3 cut(s) 274, 401, 568
ArsI GACNNNNNNTTYG 4 cut(s) 260, 292, 979, 1011
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 2 cut(s) 104, 975
AspS9I GGNCC 3 cut(s) 78, 246, 459
AsuHPI GGTGA 1 cut(s) 938
AvaII GGWCC 1 cut(s) 78
AxyI CCTNAGG 1 cut(s) 242
BanII GRGCYC 4 cut(s) 11, 42, 62, 735
BbsI GAAGAC 2 cut(s) 204, 367
Bbv12I GWGCWC 3 cut(s) 42, 240, 815
BbvI GCAGC 7 cut(s) 43, 223, 271, 469, 565, 637, 737
BccI CCATC 2 cut(s) 308, 867
BciT130I CCWGG 1 cut(s) 794
BclI TGATCA 1 cut(s) 1161
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 2 cut(s) 579, 684
BisI GCNGC 9 cut(s) 32, 212, 254, 257, 260, 483, 579, 626, 726
BlsI GCNGC 9 cut(s) 33, 213, 255, 258, 261, 484, 580, 627, 727
Bme1390I CCNGG 1 cut(s) 794
Bme18I GGWCC 1 cut(s) 78
BmgBI CACGTC 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 78, 246, 459
BmrFI CCNGG 1 cut(s) 794
BmrI ACTGGG 1 cut(s) 336
BmsI GCATC 2 cut(s) 289, 436
BmuI ACTGGG 1 cut(s) 336
BoxI GACNNNNGTC 2 cut(s) 329, 693
BpiI GAAGAC 2 cut(s) 204, 367
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 792
BsaWI WCCGGW 3 cut(s) 270, 388, 697
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 609
Bse118I RCCGGY 1 cut(s) 1147
Bse1I ACTGG 4 cut(s) 331, 940, 994, 1140
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 679
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 1 cut(s) 792
BseGI GGATG 2 cut(s) 304, 949
BseLI CCNNNNNNNGG 1 cut(s) 609
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 5 cut(s) 75, 233, 433, 800, 1020
BseNI ACTGG 4 cut(s) 331, 940, 994, 1140
BseRI GAGGAG 2 cut(s) 296, 599
BseXI GCAGC 7 cut(s) 43, 223, 271, 469, 565, 637, 737
BseYI CCCAGC 1 cut(s) 886
Bsh1236I CGCG 1 cut(s) 694
BshFI GGCC 2 cut(s) 247, 460
BsiHKAI GWGCWC 3 cut(s) 42, 240, 815
BsiSI CCGG 4 cut(s) 271, 389, 698, 1148
BslI CCNNNNNNNGG 1 cut(s) 609
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsnI GGCC 2 cut(s) 247, 460
Bsp1286I GDGCHC 6 cut(s) 11, 42, 62, 240, 735, 815
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 3 cut(s) 396, 1002, 1161
BspACI CCGC 6 cut(s) 5, 144, 254, 257, 302, 670
BspANI GGCC 2 cut(s) 247, 460
BspCNI CTCAG 5 cut(s) 74, 234, 432, 801, 1019
BspEI TCCGGA 1 cut(s) 388
BspFNI CGCG 1 cut(s) 694
BspMAI CTGCAG 1 cut(s) 583
BspQI GCTCTTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 1 cut(s) 1147
BsrI ACTGG 4 cut(s) 331, 940, 994, 1140
BssAI RCCGGY 1 cut(s) 1147
BssECI CCNNGG 1 cut(s) 792
BssMI GATC 3 cut(s) 396, 1002, 1161
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 2 cut(s) 370, 926
Bst6I CTCTTC 2 cut(s) 21, 149
BstAPI GCANNNNNTGC 1 cut(s) 517
BstC8I GCNNGC 5 cut(s) 7, 264, 522, 670, 884
BstDEI CTNAG 8 cut(s) 61, 207, 242, 419, 809, 909, 1006, 1116
BstF5I GGATG 2 cut(s) 304, 949
BstFNI CGCG 1 cut(s) 694
BstHHI GCGC 2 cut(s) 104, 975
BstKTI GATC 3 cut(s) 399, 1005, 1164
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 3 cut(s) 396, 1002, 1161
BstMWI GCNNNNNNNGC 7 cut(s) 15, 37, 244, 253, 259, 466, 517
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 1 cut(s) 515
BstPAI GACNNNNGTC 2 cut(s) 329, 693
BstSCI CCNGG 1 cut(s) 792
BstSFI CTRYAG 2 cut(s) 579, 684
BstUI CGCG 1 cut(s) 694
BstV1I GCAGC 7 cut(s) 43, 223, 271, 469, 565, 637, 737
BstV2I GAAGAC 2 cut(s) 204, 367
Bsu36I CCTNAGG 1 cut(s) 242
BsuRI GGCC 2 cut(s) 247, 460
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 2 cut(s) 304, 949
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 3 cut(s) 555, 947, 1133
Cac8I GCNNGC 5 cut(s) 7, 264, 522, 670, 884
CfoI GCGC 2 cut(s) 104, 975
Cfr10I RCCGGY 1 cut(s) 1147
Cfr13I GGNCC 3 cut(s) 78, 246, 459
CseI GACGC 4 cut(s) 634, 683, 843, 1014
Csp6I GTAC 4 cut(s) 178, 223, 364, 854
CviAII CATG 1 cut(s) 512
CviQI GTAC 4 cut(s) 178, 223, 364, 854
DdeI CTNAG 8 cut(s) 61, 207, 242, 419, 809, 909, 1006, 1116
DpnI GATC 3 cut(s) 398, 1004, 1163
DpnII GATC 3 cut(s) 396, 1002, 1161
Eam1104I CTCTTC 2 cut(s) 21, 149
EarI CTCTTC 2 cut(s) 21, 149
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 40
Eco24I GRGCYC 4 cut(s) 11, 42, 62, 735
Eco47I GGWCC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 1107
Eco81I CCTNAGG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 792
EcoT38I GRGCYC 4 cut(s) 11, 42, 62, 735
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 1 cut(s) 515
FaiI YATR 2 cut(s) 513, 737
FatI CATG 1 cut(s) 511
FauI CCCGC 1 cut(s) 677
FbaI TGATCA 1 cut(s) 1161
Fnu4HI GCNGC 9 cut(s) 32, 212, 254, 257, 260, 483, 579, 626, 726
FokI GGATG 2 cut(s) 311, 936
FriOI GRGCYC 4 cut(s) 11, 42, 62, 735
Fsp4HI GCNGC 9 cut(s) 32, 212, 254, 257, 260, 483, 579, 626, 726
FspBI CTAG 1 cut(s) 563
GlaI GCGC 2 cut(s) 103, 974
GluI GCNGC 9 cut(s) 32, 212, 254, 257, 260, 483, 579, 626, 726
GsaI CCCAGC 1 cut(s) 890
HaeIII GGCC 2 cut(s) 247, 460
HapII CCGG 4 cut(s) 271, 389, 698, 1148
HgaI GACGC 4 cut(s) 634, 683, 843, 1014
HhaI GCGC 2 cut(s) 104, 975
Hin1II CATG 1 cut(s) 515
Hin6I GCGC 2 cut(s) 102, 973
HinP1I GCGC 2 cut(s) 102, 973
HincII GTYRAC 1 cut(s) 1110
HindII GTYRAC 1 cut(s) 1110
HinfI GANTC 6 cut(s) 533, 589, 596, 689, 946, 1010
HpaII CCGG 4 cut(s) 271, 389, 698, 1148
HphI GGTGA 1 cut(s) 938
Hpy166II GTNNAC 3 cut(s) 324, 366, 1110
Hpy188I TCNGA 8 cut(s) 13, 64, 113, 408, 422, 1009, 1015, 1062
Hpy188III TCNNGA 5 cut(s) 349, 389, 431, 593, 1165
Hpy8I GTNNAC 3 cut(s) 324, 366, 1110
Hpy99I CGWCG 3 cut(s) 86, 230, 374
HpyAV CCTTC 5 cut(s) 83, 334, 706, 913, 963
HpyCH4III ACNGT 2 cut(s) 370, 926
HpyCH4IV ACGT 3 cut(s) 225, 415, 754
HpyCH4V TGCA 5 cut(s) 427, 548, 581, 1031, 1173
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 37, 244, 253, 259, 466, 517
HpyF3I CTNAG 8 cut(s) 61, 207, 242, 419, 809, 909, 1006, 1116
HpySE526I ACGT 3 cut(s) 225, 415, 754
Hsp92II CATG 1 cut(s) 515
HspAI GCGC 2 cut(s) 102, 973
Kpn2I TCCGGA 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 1161
Kzo9I GATC 3 cut(s) 396, 1002, 1161
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 4 cut(s) 37, 45, 235, 622
Lsp1109I GCAGC 7 cut(s) 43, 223, 271, 469, 565, 637, 737
LweI GCATC 2 cut(s) 289, 436
MaeI CTAG 1 cut(s) 563
MaeII ACGT 3 cut(s) 225, 415, 754
MaeIII GTNAC 2 cut(s) 124, 190
MalI GATC 3 cut(s) 398, 1004, 1163
MboI GATC 3 cut(s) 396, 1002, 1161
MboII GAAGA 6 cut(s) 35, 38, 166, 209, 367, 625
MfeI CAATTG 2 cut(s) 44, 758
MhlI GDGCHC 6 cut(s) 11, 42, 62, 240, 735, 815
MluCI AATT 9 cut(s) 44, 274, 355, 401, 503, 568, 604, 758, 797
MlyI GAGTC 1 cut(s) 683
MroI TCCGGA 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 5 cut(s) 441, 702, 870, 903, 1077
MspI CCGG 4 cut(s) 271, 389, 698, 1148
MspR9I CCNGG 1 cut(s) 794
MunI CAATTG 2 cut(s) 44, 758
MvaI CCWGG 1 cut(s) 794
MvnI CGCG 1 cut(s) 694
MwoI GCNNNNNNNGC 7 cut(s) 15, 37, 244, 253, 259, 466, 517
NdeII GATC 3 cut(s) 396, 1002, 1161
NlaIII CATG 1 cut(s) 515
NmeAIII GCCGAG 1 cut(s) 754
NmuCI GTSAC 2 cut(s) 124, 190
NspI RCATGY 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 5 cut(s) 533, 589, 596, 946, 1010
PkrI GCNGC 9 cut(s) 33, 213, 255, 258, 261, 484, 580, 627, 727
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
PshAI GACNNNNGTC 2 cut(s) 329, 693
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 1 cut(s) 792
PspPI GGNCC 3 cut(s) 78, 246, 459
PstI CTGCAG 1 cut(s) 583
RsaI GTAC 4 cut(s) 179, 224, 365, 855
RsaNI GTAC 4 cut(s) 178, 223, 364, 854
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 5 cut(s) 441, 702, 870, 903, 1077
SatI GCNGC 9 cut(s) 32, 212, 254, 257, 260, 483, 579, 626, 726
Sau3AI GATC 3 cut(s) 396, 1002, 1161
Sau96I GGNCC 3 cut(s) 78, 246, 459
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 794
SduI GDGCHC 6 cut(s) 11, 42, 62, 240, 735, 815
SfaNI GCATC 2 cut(s) 289, 436
SfcI CTRYAG 2 cut(s) 579, 684
SinI GGWCC 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 9 cut(s) 44, 274, 355, 401, 503, 568, 604, 758, 797
SsiI CCGC 6 cut(s) 5, 144, 254, 257, 302, 670
SspI AATATT 2 cut(s) 1057, 1075
SspMI CTAG 1 cut(s) 563
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 792
TaaI ACNGT 2 cut(s) 370, 926
TaiI ACGT 3 cut(s) 228, 418, 757
TaqI TCGA 6 cut(s) 174, 228, 348, 378, 399, 997
TaqII GACCGA 1 cut(s) 95
TasI AATT 9 cut(s) 44, 274, 355, 401, 503, 568, 604, 758, 797
TatI WGTACW 2 cut(s) 177, 363
TauI GCSGC 2 cut(s) 256, 259
TfiI GAWTC 5 cut(s) 533, 589, 596, 946, 1010
Tru1I TTAA 5 cut(s) 441, 702, 870, 903, 1077
Tru9I TTAA 5 cut(s) 441, 702, 870, 903, 1077
TscAI CASTG 3 cut(s) 555, 947, 1140
TseFI GTSAC 2 cut(s) 124, 190
TseI GCWGC 7 cut(s) 31, 211, 259, 482, 578, 625, 725
Tsp45I GTSAC 2 cut(s) 124, 190
TspDTI ATGAA 1 cut(s) 938
TspGWI ACGGA 2 cut(s) 209, 856
TspRI CASTG 3 cut(s) 555, 947, 1140
VpaK11BI GGWCC 1 cut(s) 78
XapI RAATTY 3 cut(s) 274, 401, 568
XceI RCATGY 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.