pycom49g00150

HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_49
OG0002980
Physical Location & Seq
Forward (+)
175671 .. 188915
13245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4119 bp
ATGGCGAAGAAGTATGTGACAACAGAGAATGCGCCCCTCTCTCAGTTCGGTGTATTGGTAGCGCAATTGGAGTCCATAGTCGCCTCAGCATCCCATAAACCTCAGGAGCCACTTCTATGTTTCGATCTCCTCTCCGATCTCATATCCGCCATTAACGAAGAGCCCAAGGAATCCATTTTGTTGTGGCAAAGAAGATGCGAGGACGCATTATATTCCTTGCTTACTCTTGGTGCTCGGCGACCTGTCCGCCATCTGGCTTCAGTTGCTATGGCGAGGGTTATATCCAAGGGAGATGGGATTTCTATATACTCTCGAGCGAGCAGTCTCCAAGGCTTTCTTTCTGATGGAAGGAGGAATGAACCACAAAAAGTTGCTGGTGCTGCACAGTGCTTAGGAGAATTATATAGACATTTTGGGAGGCGAATCACATCAGGTTTACTTGAAACGACTATCATTGCCACGAAACTTTTCAAGTTTAATGAGGAATTTGTGAGACAAGAAGCTTTATATATGCTTCAAAATGCTTTGGAAGGCTCTGGTGGAAACGCAGCAGCTTCAGCATATACAGAGGCATTTCGTCTCATCATGCGGTTTGCAGTTGGTGATAAATCATTTCTTGTTAGAATTGCTGCTGCAAGATGTCTGAAGGCATTTGCCATTATTGGAGGACCTAGTCTAGGGGTGGGAGAGCTAGACAGTTCAGCTTCTTATTGTGTCAAGAGTAGGCCCTTGAGGATCGATCCTGTTTCTTCTGTCCGTGATGCATTTGCTGAAGCATTGGGTTCGTTGCTTGCTCTTGGAATGAATCCTCATGCACAGGTTCAACCAAGGGGGAAGGGACCTTTCCCTCCAGCAAAGAAACTGGAAGGTGGTTTGCATAGACATTTAGCATTGCCTTTCACAAAAGTTGGAGCTCGGTCAAGGGATGTGCGAGTTGGTATAACCTTGTCTTGGGTATTCTTTTTACAGGCGATTCGTCTAAAATACATGCATCCAGATAGTGAGCTACAAAACTATGCAATTCAGGTTATGGAAATGCTTTGTTCCGATAATTCTGTTGATGCCCATGCACTGGCTTGTGTGCTCTATATCCTTCGTGTTGGTGTTACTGATCAAATGGCAGAGCCTACGCAAAGGAGTTTTTTGGGTTTTCTTGGGAATCAGCTTATGTCTCGAGATGCCAGTCCTTCCATGAAAATTGCCGCTCTACGTACTGCATCATATACCCTAAAAACGCTGGGAGAGGTTCCAGTTGATTTCAAGGAAGTTCTTGACAACACTGTTGTTGCAGCAGTATATCATTCTTCACAACTCGTTCGAATTGAGGCTGCTTTGACATTACGTGCCCTGGCTGAGGTTGATCCCACCTGTGTTGGTGGTTTAATTTCTTATGGAGTGACCATGCTAAATGCTTTGAGAGAAAATGTGGCATATGAGAAGGGGAGTACTTTGCAATTAGAGCTTGATTCTTTGCATGGACAAGCTACTGTCTTGGCAGCTCTGGTGTCCATTTCCCCAAAATTACCTCTCGGTTTTCCTGCCAGATTGCCCAGGTCAATCCTTGAAGTTACAAAGAAGATGCTGAATGAATCTAGTCGGAACCCAATGGCAGCTACAATTGAAAAAGAAGCTGGGTGGTTACTTTTGTCATCCCTGTTAGCTTCTATGCCAAAGGAGGAACTTGAAGACCAGGTTTTTGATATTCTTTCCTTGTGGGCTTCCCTCTTTACTGGAAATCTAGACGATGAAACTAACCAAACTGGAGATTTGACGAGTAGGATACGAATGTGGTCTGCTGCAGTTGATGCACTTACTGCATTTTTAAGATGCTTTCTTTCTCCTAATGATGGGAATAACGGGATTTTGCTTCAACCTGTACTTGTATACCTGAGTAGGGCTTTATCGTATATATCGCTGATAGCGGCCAAGCAACTGCCAAATGTGAAGCCTGCTTTGGATATATTTATCATCCGGACATTAATAGCTTACCAGTCTCTTCCTGATCCAACAGCATATAAGAATGACCACGCCATGGTTCTTCAAATTTGCACAAGCCCATTCATAGAGGCTTCTGGATGTGAAGAAAGTACATGCTTGAGGTTTCTGTTAGACAAAAGAGATGCATGGTTGGGTCCCTGGATTCCTGGAAGAGATTGGTTTGAAGATGAACTTCGTGCTTTTCAAGGTGGCAAAAACGGCCTTATTCCTTGTGTCTGGGAGAATGAAGTTTGTAGCTTTCCTCAGCCGGAACCAGTAAACAAGACATTGGTGAACCAGATGCTCCTCTGCTTTGGCGTCTTGTTTGCCTCTCAGGATAGTGGGGGGATGCTGTCACTTCTTGGAATGATTGAGCAGTGCCTGAAAGCTGGAAAAAAGCAACAATGGCATGCAGCGAGCATAACCAATATATGTGTGGGGTTACTTTCAGGATTTAAGGCTTTGCTTTCCTTGCGTCCCCAGCCATTATCACTTGAGATACTAAATTCCGCACAGGCGATATTTCAGAGCATTCTGGCAGAGGGAGACATCTGTCCATCACAACGTAGGGCTTCATCAGAATGTCTTGGTCTTTTAGCTCGTCTTGGAAATGATATCTTTACAGCAAGAATGATCGATCTGCTTGGTGACCTAACTGGGGCAACAGATTCAAACTATGCTCGATCAATTGCTTTTGCTCTTGGCTGCATTCATCGCAGTGCAGGAGGCATGGCATTGTCAACTTTAGTGCCTTCAACTGTGAGCTCAATCTCGTTGCTTTCTAAAAGTTCAATAGCTGGTTTACAGATATGGTCTTTGCATGGGCTTCTGTTGACCATTGAAGCTGCTGGCTTGTCCTTTGTATCTCAAGTCCAGGCAACACTTGGTCTTGCTCTGGAAATTCTTTTGTCCGAGGAGAATGGATGGGTTGCACTTCAGCAAGGTGTTGGTCGTCTTATAAATGCTATTGTTGCTGTTCTTGGTCCTGAACTTGCCCCTGGCAGTATTTTCTTTTCACGTTGCCAGTCTGTTGTTTCAGAGATAAGCTCTGGGCAAGAAACAGCAACGATGCTCGAGAGTGTACGCTTTACCCAGCAACTTGTTCTTTTTGCACCACAAGCTGTTTCCGTGCACACCCATGTGCAAACTCTTCTACCTACTTTGTCCTCAAGACAGCCAGCATTAAGGCATCTAGCTGTTTCCACTCTACGACATCTGATTGAAAAAGATCCAGTCTCCATTGTTGTTGAGCAAATAGAGGAGAAGTTATTTCACATGCTGGATGAAGAAACAGATTCTGAGATAGGGGATCTGGTGCGCACCACAATCATGCGATTGCTCTGTGCATCATGCCCTTCCTGTCCATCTCATTGGATATCAATTTGTCGTAATGTGATTCTTGCCACATCAACGCGAAGAAATGCTGATAGTAGTAGCAGCTTAGAAAATGATTCTTCAAAGGGTATGGAGGGTGACCCAAGTGTAAATTTTGGAGAAGCTGATGAGAACATGGTTTCTAGCACCACAGGTGTGCTCCATGGTATTCTGAACAGAGATAAACACCTCAGATACCGAACCAGAGTTTTTGCTGCTGAGTGTTTGAGTTACCTACCTAGAGCTGTTGGAAAGAATCCAGCTCATTTTGATCTCTGTACTGCTAGGAGCCAACCTACTAATAGACAGGCCTCTAGTGATTGGCTCGTCCTCCATATACAAGAACTTATAGCTCTTGCATACCAGATAAGCACGATCCAGTTGGAAAACATGCAGCCAATTGGTGTGGGGCTTCTGAGTACCATTACAGATAAGTTTGAAAAGACACCTGACCCAGAGCTTCCAGGGCATCTCCTACTAGAACAGTATCAGGCCCAGCTCGTATCTGCTGTCCGCACTGCCTTAGACTCGTCCTCTGGCCCTATTCTATTGGAAGCAGGCTTCCAGCTCGCTACGAAGATACTGACTAGTGGAATAATTAAGGGCGATCAAATTGCTGTTAAACGCGTATATTCATTAATTTCACGACCATTGAATGAATTTAAGGACCTGTATTATCCTTCATTTGCAGAATGGGTCTCATGTAAGATCAAGATAAGCCTCCTTGCTGCTCATGCCTCTCACAAATGTTATACATATGCGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1373

Amino Acids

149.61

Weight (kDa)

6.42

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Laa1_Sip1_HTR5 PF20210 1225 - 1368 4.4e-38 Laa1/Sip1/HEATR5 HEAT repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2936
Acc16I TGCGCA 1 cut(s) 3296
AccB7I CCANNNNNTGG 2 cut(s) 1072, 2032
AccBSI CCGCTC 1 cut(s) 1205
AccI GTMKAC 1 cut(s) 1884
AccII CGCG 2 cut(s) 3391, 3978
AccIII TCCGGA 1 cut(s) 1971
AciI CCGC 7 cut(s) 147, 247, 589, 1203, 1922, 2487, 3865
AclWI GGATC 7 cut(s) 734, 743, 1355, 1997, 3200, 3294, 3721
AcoI YGGCCR 1 cut(s) 1923
AcsI RAATTY 6 cut(s) 485, 2043, 2482, 2877, 3463, 4010
AcuI CTGAAG 5 cut(s) 243, 540, 665, 792, 2897
AcyI GRCGYC 1 cut(s) 2295
AfaI GTAC 7 cut(s) 1213, 1447, 1878, 2089, 3060, 3631, 3772
AfiI CCNNNNNNNGG 9 cut(s) 253, 677, 951, 1072, 1354, 1847, 1894, 2032, 2635
AflIII ACRYGT 1 cut(s) 3976
AhlI ACTAGT 1 cut(s) 3938
AjnI CCWGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
AjuI GAANNNNNNNTTGG 2 cut(s) 1937, 1969
AleI CACNNNNGTG 2 cut(s) 3116, 3506
Alw21I GWGCWC 6 cut(s) 235, 916, 1086, 2744, 3111, 3513
Alw26I GTCTC 8 cut(s) 329, 487, 584, 1176, 1998, 2517, 3217, 4054
Alw44I GTGCAC 1 cut(s) 3107
AlwI GGATC 7 cut(s) 734, 743, 1355, 1997, 3200, 3294, 3721
AlwNI CAGNNNCTG 2 cut(s) 2359, 3275
Ama87I CYCGRG 3 cut(s) 312, 1173, 3050
Aor13HI TCCGGA 1 cut(s) 1971
AoxI GGCC 6 cut(s) 725, 1923, 2197, 3660, 3843, 3889
ApaLI GTGCAC 1 cut(s) 3107
ApoI RAATTY 6 cut(s) 485, 2043, 2482, 2877, 3463, 4010
ArsI GACNNNNNNTTYG 2 cut(s) 1679, 1711
AseI ATTAAT 2 cut(s) 1979, 3989
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 3 cut(s) 34, 64, 3297
AspS9I GGNCC 8 cut(s) 668, 726, 839, 2132, 2960, 3844, 3890, 4018
AsuHPI GGTGA 4 cut(s) 614, 2281, 2636, 3461
AsuII TTCGAA 1 cut(s) 1318
AvaI CYCGRG 3 cut(s) 312, 1173, 3050
AvaII GGWCC 5 cut(s) 668, 839, 2132, 2960, 4018
AxyI CCTNAGG 1 cut(s) 102
BaeGI GKGCMC 2 cut(s) 1348, 3111
BaeI ACNNNNGTAYC 2 cut(s) 1772, 1805
BanII GRGCYC 3 cut(s) 165, 916, 2744
BbsI GAAGAC 1 cut(s) 1692
Bbv12I GWGCWC 6 cut(s) 235, 916, 1086, 2744, 3111, 3513
BbvCI CCTCAGC 3 cut(s) 85, 1353, 2241
BccI CCATC 7 cut(s) 258, 287, 338, 1841, 2542, 2895, 3349
BceAI ACGGC 1 cut(s) 2212
BcgI CGANNNNNNTGC 4 cut(s) 765, 799, 3947, 3981
BciT130I CCWGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
BciVI GTATCC 1 cut(s) 1773
BclI TGATCA 1 cut(s) 1111
BcoDI GTCTC 8 cut(s) 329, 487, 584, 1176, 1998, 2517, 3217, 4054
BcuI ACTAGT 1 cut(s) 3938
BfmI CTRYAG 1 cut(s) 1797
BfuI GTATCC 1 cut(s) 1773
BmcAI AGTACT 1 cut(s) 1447
Bme1390I CCNGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
Bme18I GGWCC 5 cut(s) 668, 839, 2132, 2960, 4018
BmeT110I CYCGRG 3 cut(s) 312, 1173, 3050
BmgT120I GGNCC 8 cut(s) 668, 726, 839, 2132, 2960, 3844, 3890, 4018
BmiI GGNNCC 8 cut(s) 108, 840, 1248, 1601, 2133, 2134, 2250, 3641
BmrFI CCNGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
BmrI ACTGGG 1 cut(s) 2643
BmuI ACTGGG 1 cut(s) 2643
BoxI GACNNNNGTC 1 cut(s) 2529
BpiI GAAGAC 1 cut(s) 1692
BplI GAGNNNNNCTC 2 cut(s) 3008, 3040
BpmI CTGGAG 2 cut(s) 834, 1782
Bpu10I CCTNAGC 4 cut(s) 85, 391, 1353, 2241
Bpu14I TTCGAA 1 cut(s) 1318
BpuEI CTTGAG 5 cut(s) 751, 2116, 2492, 2829, 3130
Bsa29I ATCGAT 2 cut(s) 738, 2613
BsaAI YACGTR 2 cut(s) 1211, 1343
BsaHI GRCGYC 1 cut(s) 2295
BsaI GGTCTC 1 cut(s) 4054
BsaWI WCCGGW 1 cut(s) 1971
Bsc4I CCNNNNNNNGG 9 cut(s) 253, 677, 951, 1072, 1354, 1847, 1894, 2032, 2635
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 2 cut(s) 453, 890
BseAI TCCGGA 1 cut(s) 1971
BseBI CCWGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
BseCI ATCGAT 2 cut(s) 738, 2613
BseGI GGATG 9 cut(s) 89, 931, 991, 1649, 1968, 2081, 2331, 2906, 3265
BseLI CCNNNNNNNGG 9 cut(s) 253, 677, 951, 1072, 1354, 1847, 1894, 2032, 2635
BseMI GCAATG 2 cut(s) 453, 890
BseRI GAGGAG 4 cut(s) 119, 2273, 2906, 3251
BseSI GKGCMC 2 cut(s) 1348, 3111
BseYI CCCAGC 5 cut(s) 1237, 1631, 2457, 3069, 3846
BsgI GTGCAG 2 cut(s) 366, 2718
Bsh1236I CGCG 2 cut(s) 3391, 3978
BshFI GGCC 6 cut(s) 727, 1925, 2199, 3662, 3845, 3891
BshVI ATCGAT 2 cut(s) 738, 2613
BsiHKAI GWGCWC 6 cut(s) 235, 916, 1086, 2744, 3111, 3513
BsiHKCI CYCGRG 3 cut(s) 312, 1173, 3050
BsiSI CCGG 2 cut(s) 1972, 2246
BslFI GGGAC 3 cut(s) 852, 2118, 2439
BslI CCNNNNNNNGG 9 cut(s) 253, 677, 951, 1072, 1354, 1847, 1894, 2032, 2635
BsmAI GTCTC 8 cut(s) 329, 487, 584, 1176, 1998, 2517, 3217, 4054
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 3 cut(s) 852, 2118, 2439
BsmI GAATGC 3 cut(s) 34, 2508, 2685
BsnI GGCC 6 cut(s) 727, 1925, 2199, 3662, 3845, 3891
Bso31I GGTCTC 1 cut(s) 4054
BsoBI CYCGRG 3 cut(s) 312, 1173, 3050
Bsp119I TTCGAA 1 cut(s) 1318
Bsp1286I GDGCHC 8 cut(s) 165, 235, 916, 1086, 1348, 2744, 3111, 3513
Bsp13I TCCGGA 1 cut(s) 1971
Bsp19I CCATGG 2 cut(s) 2031, 3514
BspACI CCGC 7 cut(s) 147, 247, 589, 1203, 1922, 2487, 3865
BspANI GGCC 6 cut(s) 727, 1925, 2199, 3662, 3845, 3891
BspDI ATCGAT 2 cut(s) 738, 2613
BspEI TCCGGA 1 cut(s) 1971
BspFNI CGCG 2 cut(s) 3391, 3978
BspLI GGNNCC 8 cut(s) 108, 840, 1248, 1601, 2133, 2134, 2250, 3641
BspMAI CTGCAG 1 cut(s) 1801
BspPI GGATC 7 cut(s) 734, 743, 1355, 1997, 3200, 3294, 3721
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 1318
BspTNI GGTCTC 1 cut(s) 4054
BsrBI CCGCTC 1 cut(s) 1205
BsrDI GCAATG 2 cut(s) 453, 890
BssNAI GTATAC 1 cut(s) 1885
BssNI GRCGYC 1 cut(s) 2295
BssT1I CCWWGG 6 cut(s) 165, 285, 328, 827, 2031, 3514
Bst1107I GTATAC 1 cut(s) 1885
Bst2UI CCWGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
Bst4CI ACNGT 6 cut(s) 387, 698, 1282, 1489, 2737, 3837
Bst6I CTCTTC 4 cut(s) 153, 2001, 2143, 3132
BstACI GRCGYC 1 cut(s) 2295
BstAPI GCANNNNNTGC 2 cut(s) 1805, 1814
BstBAI YACGTR 2 cut(s) 1211, 1343
BstBI TTCGAA 1 cut(s) 1318
BstC8I GCNNGC 9 cut(s) 319, 792, 1950, 2388, 2395, 2827, 3156, 3910, 3921
BstDSI CCRYGG 2 cut(s) 2031, 3514
BstEII GGTNACC 2 cut(s) 2624, 3449
BstENI CCTNNNNNAGG 2 cut(s) 675, 1352
BstF5I GGATG 9 cut(s) 89, 931, 991, 1649, 1968, 2081, 2331, 2906, 3265
BstFNI CGCG 2 cut(s) 3391, 3978
BstHHI GCGC 3 cut(s) 34, 64, 3297
BstMAI GTCTC 8 cut(s) 329, 487, 584, 1176, 1998, 2517, 3217, 4054
BstNI CCWGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
BstNSI RCATGY 5 cut(s) 991, 2094, 2390, 3256, 3745
BstPAI GACNNNNGTC 1 cut(s) 2529
BstPI GGTNACC 2 cut(s) 2624, 3449
BstSCI CCNGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
BstSFI CTRYAG 1 cut(s) 1797
BstSLI GKGCMC 2 cut(s) 1348, 3111
BstSNI TACGTA 1 cut(s) 1211
BstUI CGCG 2 cut(s) 3391, 3978
BstV2I GAAGAC 1 cut(s) 1692
BstX2I RGATCY 2 cut(s) 3205, 3286
BstXI CCANNNNNNTGG 1 cut(s) 3348
BstYI RGATCY 2 cut(s) 3205, 3286
BstZ17I GTATAC 1 cut(s) 1885
Bsu15I ATCGAT 2 cut(s) 738, 2613
Bsu36I CCTNAGG 1 cut(s) 102
BsuI GTATCC 1 cut(s) 1773
BsuRI GGCC 6 cut(s) 727, 1925, 2199, 3662, 3845, 3891
BsuTUI ATCGAT 2 cut(s) 738, 2613
BtgI CCRYGG 2 cut(s) 2031, 3514
BtgZI GCGATG 1 cut(s) 2675
BtsCI GGATG 9 cut(s) 89, 931, 991, 1649, 1968, 2081, 2331, 2906, 3265
BtsI GCAGTG 3 cut(s) 2360, 2701, 3867
BtsIMutI CAGTG 6 cut(s) 392, 1070, 1278, 2360, 2701, 3867
Cac8I GCNNGC 9 cut(s) 319, 792, 1950, 2388, 2395, 2827, 3156, 3910, 3921
CaiI CAGNNNCTG 2 cut(s) 2359, 3275
CfoI GCGC 3 cut(s) 34, 64, 3297
Cfr13I GGNCC 8 cut(s) 668, 726, 839, 2132, 2960, 3844, 3890, 4018
ClaI ATCGAT 2 cut(s) 738, 2613
CseI GACGC 3 cut(s) 212, 2284, 2441
CsiI ACCWGGT 1 cut(s) 1689
Csp6I GTAC 7 cut(s) 1212, 1446, 1877, 2088, 3059, 3630, 3771
CspCI CAANNNNNGTGG 4 cut(s) 1354, 1389, 3738, 3773
CviQI GTAC 7 cut(s) 1212, 1446, 1877, 2088, 3059, 3630, 3771
EaeI YGGCCR 1 cut(s) 1923
Eam1104I CTCTTC 4 cut(s) 153, 2001, 2143, 3132
EarI CTCTTC 4 cut(s) 153, 2001, 2143, 3132
EciI GGCGGA 2 cut(s) 136, 236
Ecl136II GAGCTC 2 cut(s) 914, 2742
Eco105I TACGTA 1 cut(s) 1211
Eco130I CCWWGG 6 cut(s) 165, 285, 328, 827, 2031, 3514
Eco147I AGGCCT 1 cut(s) 3662
Eco24I GRGCYC 3 cut(s) 165, 916, 2744
Eco31I GGTCTC 1 cut(s) 4054
Eco32I GATATC 2 cut(s) 2593, 3354
Eco47I GGWCC 5 cut(s) 668, 839, 2132, 2960, 4018
Eco53kI GAGCTC 2 cut(s) 914, 2742
Eco57I CTGAAG 5 cut(s) 243, 540, 665, 792, 2897
Eco81I CCTNAGG 1 cut(s) 102
Eco88I CYCGRG 3 cut(s) 312, 1173, 3050
Eco91I GGTNACC 2 cut(s) 2624, 3449
EcoICRI GAGCTC 2 cut(s) 914, 2742
EcoNI CCTNNNNNAGG 2 cut(s) 675, 1352
EcoO109I RGGNCCY 5 cut(s) 668, 726, 839, 2132, 4018
EcoO65I GGTNACC 2 cut(s) 2624, 3449
EcoRII CCWGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
EcoRV GATATC 2 cut(s) 2593, 3354
EcoT14I CCWWGG 6 cut(s) 165, 285, 328, 827, 2031, 3514
EcoT22I ATGCAT 3 cut(s) 766, 993, 2125
EcoT38I GRGCYC 3 cut(s) 165, 916, 2744
ErhI CCWWGG 6 cut(s) 165, 285, 328, 827, 2031, 3514
Esp3I CGTCTC 1 cut(s) 584
FalI AAGNNNNNCTT 4 cut(s) 321, 353, 2154, 2186
FaqI GGGAC 3 cut(s) 852, 2118, 2439
FauNDI CATATG 2 cut(s) 1432, 4108
FbaI TGATCA 1 cut(s) 1111
FblI GTMKAC 1 cut(s) 1884
FokI GGATG 9 cut(s) 76, 938, 978, 1636, 1955, 2088, 2338, 2913, 3272
FriOI GRGCYC 3 cut(s) 165, 916, 2744
FspAI RTGCGCAY 1 cut(s) 3296
FspI TGCGCA 1 cut(s) 3296
GlaI GCGC 3 cut(s) 33, 63, 3296
GsaI CCCAGC 5 cut(s) 1241, 1635, 2461, 3073, 3850
GsuI CTGGAG 2 cut(s) 834, 1782
HaeIII GGCC 6 cut(s) 727, 1925, 2199, 3662, 3845, 3891
HapII CCGG 2 cut(s) 1972, 2246
HgaI GACGC 3 cut(s) 212, 2284, 2441
HhaI GCGC 3 cut(s) 34, 64, 3297
Hin1I GRCGYC 1 cut(s) 2295
Hin6I GCGC 3 cut(s) 32, 62, 3295
HinP1I GCGC 3 cut(s) 32, 62, 3295
HincII GTYRAC 2 cut(s) 2718, 2811
HindII GTYRAC 2 cut(s) 2718, 2811
HindIII AAGCTT 1 cut(s) 501
HpaII CCGG 2 cut(s) 1972, 2246
HphI GGTGA 4 cut(s) 614, 2281, 2636, 3461
Hpy166II GTNNAC 9 cut(s) 437, 1885, 2257, 2272, 2718, 2780, 2811, 3059, 3109
Hpy8I GTNNAC 9 cut(s) 437, 1885, 2257, 2272, 2718, 2780, 2811, 3059, 3109
HpyCH4III ACNGT 6 cut(s) 387, 698, 1282, 1489, 2737, 3837
HpyCH4IV ACGT 4 cut(s) 1210, 1342, 2542, 2995
HpySE526I ACGT 4 cut(s) 1210, 1342, 2542, 2995
Hsp92I GRCGYC 1 cut(s) 2295
HspAI GCGC 3 cut(s) 32, 62, 3295
KflI GGGWCCC 1 cut(s) 2132
Kpn2I TCCGGA 1 cut(s) 1971
Ksp22I TGATCA 1 cut(s) 1111
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 5 cut(s) 106, 911, 2286, 3516, 3639
MabI ACCWGGT 1 cut(s) 1689
MaeII ACGT 4 cut(s) 1210, 1342, 2542, 2995
MbiI CCGCTC 1 cut(s) 1205
MfeI CAATTG 4 cut(s) 65, 1617, 2664, 3750
MflI RGATCY 2 cut(s) 3205, 3286
MhlI GDGCHC 8 cut(s) 165, 235, 916, 1086, 1348, 2744, 3111, 3513
MluI ACGCGT 1 cut(s) 3976
MlyI GAGTC 2 cut(s) 80, 3872
MmeI TCCRAC 5 cut(s) 889, 1577, 2030, 3580, 3714
Mph1103I ATGCAT 3 cut(s) 766, 993, 2125
MroI TCCGGA 1 cut(s) 1971
MroXI GAANNNNTTC 1 cut(s) 467
MslI CAYNNNNRTG 7 cut(s) 115, 2557, 2694, 3114, 3116, 3305, 3506
MspI CCGG 2 cut(s) 1972, 2246
MspR9I CCNGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
MunI CAATTG 4 cut(s) 65, 1617, 2664, 3750
Mva1269I GAATGC 3 cut(s) 34, 2508, 2685
MvaI CCWGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
MvnI CGCG 2 cut(s) 3391, 3978
NcoI CCATGG 2 cut(s) 2031, 3514
NdeI CATATG 2 cut(s) 1432, 4108
NlaIV GGNNCC 8 cut(s) 108, 840, 1248, 1601, 2133, 2134, 2250, 3641
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 5 cut(s) 16, 1396, 2331, 2624, 3449
NsbI TGCGCA 1 cut(s) 3296
NsiI ATGCAT 3 cut(s) 766, 993, 2125
NspI RCATGY 5 cut(s) 991, 2094, 2390, 3256, 3745
NspV TTCGAA 1 cut(s) 1318
OliI CACNNNNGTG 2 cut(s) 3116, 3506
PaeI GCATGC 1 cut(s) 2390
PaeR7I CTCGAG 3 cut(s) 312, 1173, 3050
PceI AGGCCT 1 cut(s) 3662
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 3 cut(s) 34, 2508, 2685
PdmI GAANNNNTTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 672
PflMI CCANNNNNTGG 2 cut(s) 1072, 2032
PfoI TCCNGGA 1 cut(s) 2143
PleI GAGTC 2 cut(s) 79, 3872
PpsI GAGTC 2 cut(s) 79, 3872
Ppu21I YACGTR 2 cut(s) 1211, 1343
PpuMI RGGWCCY 4 cut(s) 668, 839, 2132, 4018
PshAI GACNNNNGTC 1 cut(s) 2529
PshBI ATTAAT 2 cut(s) 1979, 3989
PsiI TTATAA 1 cut(s) 2936
Psp124BI GAGCTC 2 cut(s) 916, 2744
Psp5II RGGWCCY 4 cut(s) 668, 839, 2132, 4018
Psp6I CCWGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
PspEI GGTNACC 2 cut(s) 2624, 3449
PspFI CCCAGC 5 cut(s) 1237, 1631, 2457, 3069, 3846
PspGI CCWGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
PspN4I GGNNCC 8 cut(s) 108, 840, 1248, 1601, 2133, 2134, 2250, 3641
PspPI GGNCC 8 cut(s) 668, 726, 839, 2132, 2960, 3844, 3890, 4018
PspPPI RGGWCCY 4 cut(s) 668, 839, 2132, 4018
PstI CTGCAG 1 cut(s) 1801
PstNI CAGNNNCTG 2 cut(s) 2359, 3275
PsuI RGATCY 2 cut(s) 3205, 3286
PsyI GACNNNGTC 1 cut(s) 672
RsaI GTAC 7 cut(s) 1213, 1447, 1878, 2089, 3060, 3631, 3772
RsaNI GTAC 7 cut(s) 1212, 1446, 1877, 2088, 3059, 3630, 3771
RseI CAYNNNNRTG 7 cut(s) 115, 2557, 2694, 3114, 3116, 3305, 3506
SacI GAGCTC 2 cut(s) 916, 2744
SapI GCTCTTC 1 cut(s) 153
Sau96I GGNCC 8 cut(s) 668, 726, 839, 2132, 2960, 3844, 3890, 4018
ScaI AGTACT 1 cut(s) 1447
SchI GAGTC 2 cut(s) 80, 3872
ScrFI CCNGG 8 cut(s) 1349, 1552, 1691, 2137, 2145, 2852, 2976, 3816
SduI GDGCHC 8 cut(s) 165, 235, 916, 1086, 1348, 2744, 3111, 3513
SexAI ACCWGGT 1 cut(s) 1689
SfcI CTRYAG 1 cut(s) 1797
Sfr274I CTCGAG 3 cut(s) 312, 1173, 3050
SfuI TTCGAA 1 cut(s) 1318
SinI GGWCC 5 cut(s) 668, 839, 2132, 2960, 4018
SlaI CTCGAG 3 cut(s) 312, 1173, 3050
SmiMI CAYNNNNRTG 7 cut(s) 115, 2557, 2694, 3114, 3116, 3305, 3506
SmlI CTYRAG 8 cut(s) 312, 730, 1173, 2095, 2471, 2844, 3050, 3145
SmoI CTYRAG 8 cut(s) 312, 730, 1173, 2095, 2471, 2844, 3050, 3145
SnaBI TACGTA 1 cut(s) 1211
SpeI ACTAGT 1 cut(s) 3938
SphI GCATGC 1 cut(s) 2390
SseBI AGGCCT 1 cut(s) 3662
SsiI CCGC 7 cut(s) 147, 247, 589, 1203, 1922, 2487, 3865
SstI GAGCTC 2 cut(s) 916, 2744
StuI AGGCCT 1 cut(s) 3662
StyD4I CCNGG 8 cut(s) 1347, 1550, 1689, 2135, 2143, 2850, 2974, 3814
StyI CCWWGG 6 cut(s) 165, 285, 328, 827, 2031, 3514
TaaI ACNGT 6 cut(s) 387, 698, 1282, 1489, 2737, 3837
TaiI ACGT 4 cut(s) 1213, 1345, 2545, 2998
TaqI TCGA 8 cut(s) 123, 313, 738, 1174, 1318, 2613, 2659, 3051
TaqII GACCGA 1 cut(s) 906
TatI WGTACW 4 cut(s) 1445, 1876, 2087, 3629
TauI GCSGC 2 cut(s) 1205, 1925
TscAI CASTG 6 cut(s) 392, 1077, 1285, 2360, 2701, 3874
TseFI GTSAC 5 cut(s) 16, 1396, 2331, 2624, 3449
Tsp45I GTSAC 5 cut(s) 16, 1396, 2331, 2624, 3449
TspGWI ACGGA 2 cut(s) 746, 3094
TspRI CASTG 6 cut(s) 392, 1077, 1285, 2360, 2701, 3874
Tth111I GACNNNGTC 1 cut(s) 672
Van91I CCANNNNNTGG 2 cut(s) 1072, 2032
VneI GTGCAC 1 cut(s) 3107
VpaK11BI GGWCC 5 cut(s) 668, 839, 2132, 2960, 4018
VspI ATTAAT 2 cut(s) 1979, 3989
XagI CCTNNNNNAGG 2 cut(s) 675, 1352
XapI RAATTY 6 cut(s) 485, 2043, 2482, 2877, 3463, 4010
XbaI TCTAGA 1 cut(s) 1738
XceI RCATGY 5 cut(s) 991, 2094, 2390, 3256, 3745
XcmI CCANNNNNNNNNTGG 2 cut(s) 2410, 2858
XhoI CTCGAG 3 cut(s) 312, 1173, 3050
XmiI GTMKAC 1 cut(s) 1884
XmnI GAANNNNTTC 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 1447
Zsp2I ATGCAT 3 cut(s) 766, 993, 2125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.