RchiOBHm_Chr6g0249471

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0016165
Physical Location & Seq
Reverse (-)
5432375 .. 5433974
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1182 bp
ATGCATGCATGCATGGGTGGTAAGAAGATGGTAGCGATTTGTAATGACCGGCTGTGGTTAGCCAATTATACTATCGGAATGGTGTTTACAATGATCTTAATTAATAGTAGTAGTATGGTGGCCGGCATGGGATTTGGGGAGTCAGCTGCGGCGGCGGCGGCGGCGGCGGCGCAGGTTCCGGCAATGTTTGCGTTTGGCGACTCACTCATTGATGCTGGCAACAACAACTTCATAAGCTCCATCGCAAAATCCAATTATTATCCTTACGGCATCGATTTCAGGGGAGGCCCCTCCGGCAGATTCACCAATGGAAGAACTTTCGTTGATATGCTTGGAAATTTGCTGGGTCTTCCTTATGCTCCAGCCTTTGCAGATCCTAATACAAAAGGAAACAGAATGCTCAGGGGAGTCAATTATGCTTCAGCAGCTGCTGGCATACTCGATGAGACAGGCCGGCACTATGGAGATCGGTATACCCTAAGCCGGCAAGTGTTGAATTTCGAGACCACCTTGAATCAGTTAAGAACGATGATGGGGGGAACTAACCTGACCCAGTACCTGGCTAAGTCGATTGCCGTTTTGGTATTTGGGAGCAATGACTACATCAACAACTACCTCATGCCTTCCTTCTATTCTTCCAGCTACACTTACACTCCTCTTGCCTTTGCCAACCTTCTCCTTAATCGCTATGCCACACAGATTCTGGCTTTGCACGGTCTTGGATTGAGGAAGTTTGTGCTAGTAGGAATTGGGCCTTTGGGTTGCATTCCTAACCAAAGAGCATCAGGTAAGGCCCAGCCTGGAAGGTGTGTTGACAATGTCAATCAGATCCTGGGCAGCTTCAATCAAGGGCTCAGATCGCTCGTCACCCAACTTAACAACAACCATCCTGGTGCCATCTTTGTGTACGGCAATGCTTATGGCGCTTTTGGTGACATTCTGAATAACCCTGCAGCCTATGGTTTCAGTGTGATTGATAGGGCTTGCTGTGGAATTGGGAGGAACCAAGGGCAGATAACTTGTCTTCCATTTGCAGTTCCATGCTCAAATAGAAATCAATATATGTTTTGGGATGCTTTCCACCCAACTGAAGCTGCAAACGCAGTCCTTGCTTGGCGAGCTTTTAATGGCCCGCCCTCTGATAGTTACCCCATCAATGTCCAACAAATGGCTCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

393

Amino Acids

42.39

Weight (kDa)

9.05

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 63 - 371 1.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 893
AccB7I CCANNNNNTGG 2 cut(s) 559, 1168
AccI GTMKAC 1 cut(s) 473
AciI CCGC 8 cut(s) 149, 152, 155, 158, 161, 164, 167, 1133
AclWI GGATC 2 cut(s) 368, 823
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 2 cut(s) 337, 496
AcuI CTGAAG 2 cut(s) 405, 1110
AfaI GTAC 2 cut(s) 557, 908
AfiI CCNNNNNNNGG 3 cut(s) 483, 559, 1168
AgsI TTSAA 3 cut(s) 496, 514, 844
AjnI CCWGG 4 cut(s) 558, 799, 831, 889
AluBI AGCT 7 cut(s) 146, 237, 428, 642, 840, 1094, 1121
AluI AGCT 7 cut(s) 146, 237, 428, 642, 840, 1094, 1121
Alw26I GTCTC 2 cut(s) 440, 497
AlwI GGATC 2 cut(s) 368, 823
AlwNI CAGNNNCTG 5 cut(s) 428, 431, 559, 703, 832
AoxI GGCC 6 cut(s) 120, 286, 451, 752, 792, 1129
ApeKI GCWGC 6 cut(s) 146, 425, 428, 837, 953, 1094
ApoI RAATTY 2 cut(s) 337, 496
AseI ATTAAT 1 cut(s) 102
AspLEI GCGC 2 cut(s) 172, 926
AspS9I GGNCC 4 cut(s) 287, 752, 793, 1130
AsuHPI GGTGA 3 cut(s) 295, 859, 944
BanI GGYRCC 1 cut(s) 893
BanII GRGCYC 1 cut(s) 855
BbsI GAAGAC 2 cut(s) 341, 1016
BbvI GCAGC 6 cut(s) 133, 415, 437, 849, 965, 1081
BccI CCATC 6 cut(s) 22, 248, 526, 894, 905, 1160
BceAI ACGGC 3 cut(s) 283, 560, 925
BciT130I CCWGG 4 cut(s) 560, 801, 833, 891
BcoDI GTCTC 2 cut(s) 440, 497
BfaI CTAG 1 cut(s) 740
BfmI CTRYAG 1 cut(s) 951
BfoI RGCGCY 1 cut(s) 927
BfuAI ACCTGC 1 cut(s) 163
BglI GCCNNNNNGGC 1 cut(s) 294
Bme1390I CCNGG 4 cut(s) 560, 801, 833, 891
BmgT120I GGNCC 4 cut(s) 287, 752, 793, 1130
BmiI GGNNCC 4 cut(s) 177, 289, 895, 1004
BmrFI CCNGG 4 cut(s) 560, 801, 833, 891
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 4 cut(s) 202, 279, 791, 1063
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 2 cut(s) 341, 1016
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 2 cut(s) 401, 479
Bsa29I ATCGAT 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 2 cut(s) 832, 1006
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 3 cut(s) 483, 559, 1168
Bse118I RCCGGY 4 cut(s) 48, 122, 453, 483
Bse1I ACTGG 1 cut(s) 553
Bse3DI GCAATG 3 cut(s) 189, 601, 919
BseBI CCWGG 4 cut(s) 560, 801, 833, 891
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 832, 1006
BseGI GGATG 2 cut(s) 886, 1078
BseLI CCNNNNNNNGG 3 cut(s) 483, 559, 1168
BseMI GCAATG 3 cut(s) 189, 601, 919
BseMII CTCAG 2 cut(s) 415, 868
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 1 cut(s) 645
BseXI GCAGC 6 cut(s) 133, 415, 437, 849, 965, 1081
BseYI CCCAGC 2 cut(s) 343, 795
BshFI GGCC 6 cut(s) 122, 288, 453, 754, 794, 1131
BshNI GGYRCC 1 cut(s) 893
BshVI ATCGAT 1 cut(s) 273
BsiSI CCGG 6 cut(s) 49, 123, 179, 294, 454, 484
BslI CCNNNNNNNGG 3 cut(s) 483, 559, 1168
BsmAI GTCTC 2 cut(s) 440, 497
BsmI GAATGC 2 cut(s) 402, 765
BsnI GGCC 6 cut(s) 122, 288, 453, 754, 794, 1131
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 5 cut(s) 93, 373, 466, 828, 857
BspACI CCGC 8 cut(s) 149, 152, 155, 158, 161, 164, 167, 1133
BspANI GGCC 6 cut(s) 122, 288, 453, 754, 794, 1131
BspCNI CTCAG 2 cut(s) 414, 867
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 4 cut(s) 177, 289, 895, 1004
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 2 cut(s) 368, 823
BspT107I GGYRCC 1 cut(s) 893
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 3 cut(s) 189, 601, 919
BsrFI RCCGGY 4 cut(s) 48, 122, 453, 483
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 4 cut(s) 48, 122, 453, 483
BssECI CCNNGG 2 cut(s) 832, 1006
BssMI GATC 5 cut(s) 93, 373, 466, 828, 857
BssNAI GTATAC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 1006
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 4 cut(s) 560, 801, 833, 891
Bst4CI ACNGT 1 cut(s) 716
BstAPI GCANNNNNTGC 2 cut(s) 188, 1109
BstDEI CTNAG 4 cut(s) 401, 479, 564, 854
BstF5I GGATG 2 cut(s) 886, 1078
BstH2I RGCGCY 1 cut(s) 927
BstHHI GCGC 2 cut(s) 172, 926
BstKTI GATC 5 cut(s) 96, 376, 469, 831, 860
BstMAI GTCTC 2 cut(s) 440, 497
BstMBI GATC 5 cut(s) 93, 373, 466, 828, 857
BstNI CCWGG 4 cut(s) 560, 801, 833, 891
BstNSI RCATGY 2 cut(s) 8, 12
BstSCI CCNGG 4 cut(s) 558, 799, 831, 889
BstSFI CTRYAG 1 cut(s) 951
BstV1I GCAGC 6 cut(s) 133, 415, 437, 849, 965, 1081
BstV2I GAAGAC 2 cut(s) 341, 1016
BstX2I RGATCY 2 cut(s) 373, 828
BstYI RGATCY 2 cut(s) 373, 828
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 6 cut(s) 122, 288, 453, 754, 794, 1131
BsuTUI ATCGAT 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 2 cut(s) 886, 1078
BtsIMutI CAGTG 1 cut(s) 973
BveI ACCTGC 1 cut(s) 163
CaiI CAGNNNCTG 5 cut(s) 428, 431, 559, 703, 832
CfoI GCGC 2 cut(s) 172, 926
Cfr10I RCCGGY 4 cut(s) 48, 122, 453, 483
Cfr13I GGNCC 4 cut(s) 287, 752, 793, 1130
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 556, 907
CviAII CATG 6 cut(s) 5, 9, 13, 127, 619, 1041
CviQI GTAC 2 cut(s) 556, 907
DdeI CTNAG 4 cut(s) 401, 479, 564, 854
DpnI GATC 5 cut(s) 95, 375, 468, 830, 859
DpnII GATC 5 cut(s) 93, 373, 466, 828, 857
EaeI YGGCCR 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 1006
Eco24I GRGCYC 1 cut(s) 855
Eco31I GGTCTC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 405, 1110
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 4 cut(s) 558, 799, 831, 889
EcoT14I CCWWGG 1 cut(s) 1006
EcoT22I ATGCAT 3 cut(s) 6, 10, 14
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 1006
FaeI CATG 6 cut(s) 8, 12, 16, 130, 622, 1044
FatI CATG 6 cut(s) 4, 8, 12, 126, 618, 1040
FauI CCCGC 1 cut(s) 1140
FblI GTMKAC 1 cut(s) 473
FokI GGATG 2 cut(s) 873, 1085
FriOI GRGCYC 1 cut(s) 855
FspBI CTAG 1 cut(s) 740
GlaI GCGC 2 cut(s) 171, 925
GsaI CCCAGC 2 cut(s) 347, 799
GsuI CTGGAG 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 927
HaeIII GGCC 6 cut(s) 122, 288, 453, 754, 794, 1131
HapII CCGG 6 cut(s) 49, 123, 179, 294, 454, 484
HhaI GCGC 2 cut(s) 172, 926
Hin1II CATG 6 cut(s) 8, 12, 16, 130, 622, 1044
Hin6I GCGC 2 cut(s) 170, 924
HinP1I GCGC 2 cut(s) 170, 924
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HinfI GANTC 6 cut(s) 140, 200, 300, 408, 514, 700
HpaII CCGG 6 cut(s) 49, 123, 179, 294, 454, 484
HphI GGTGA 3 cut(s) 295, 859, 944
Hpy166II GTNNAC 4 cut(s) 87, 474, 814, 907
Hpy188I TCNGA 5 cut(s) 77, 828, 857, 942, 1141
Hpy188III TCNNGA 1 cut(s) 502
Hpy8I GTNNAC 4 cut(s) 87, 474, 814, 907
HpyAV CCTTC 4 cut(s) 633, 637, 683, 798
HpyCH4III ACNGT 1 cut(s) 716
HpyCH4V TGCA 9 cut(s) 4, 8, 12, 371, 712, 765, 953, 1034, 1097
HpyF3I CTNAG 4 cut(s) 401, 479, 564, 854
Hsp92II CATG 6 cut(s) 8, 12, 16, 130, 622, 1044
HspAI GCGC 2 cut(s) 170, 924
KroI GCCGGC 3 cut(s) 122, 453, 483
KroNI GCCGGC 3 cut(s) 124, 455, 485
Kzo9I GATC 5 cut(s) 93, 373, 466, 828, 857
LmnI GCTCC 3 cut(s) 242, 364, 591
Lsp1109I GCAGC 6 cut(s) 133, 415, 437, 849, 965, 1081
LweI GCATC 4 cut(s) 202, 279, 791, 1063
MaeI CTAG 1 cut(s) 740
MaeIII GTNAC 3 cut(s) 865, 932, 1145
MalI GATC 5 cut(s) 95, 375, 468, 830, 859
MboI GATC 5 cut(s) 93, 373, 466, 828, 857
MboII GAAGA 5 cut(s) 37, 324, 341, 627, 1016
MflI RGATCY 2 cut(s) 373, 828
MhlI GDGCHC 1 cut(s) 855
MluCI AATT 8 cut(s) 64, 99, 253, 337, 412, 496, 747, 993
MlyI GAGTC 3 cut(s) 149, 194, 417
MnlI CCTC 7 cut(s) 278, 301, 626, 666, 720, 993, 1147
Mph1103I ATGCAT 3 cut(s) 6, 10, 14
MroNI GCCGGC 3 cut(s) 122, 453, 483
MseI TTAA 6 cut(s) 98, 102, 521, 681, 876, 1125
MslI CAYNNNNRTG 2 cut(s) 891, 902
MspA1I CMGCKG 2 cut(s) 146, 428
MspI CCGG 6 cut(s) 49, 123, 179, 294, 454, 484
MspR9I CCNGG 4 cut(s) 560, 801, 833, 891
Mva1269I GAATGC 2 cut(s) 402, 765
MvaI CCWGG 4 cut(s) 560, 801, 833, 891
NaeI GCCGGC 3 cut(s) 124, 455, 485
NdeII GATC 5 cut(s) 93, 373, 466, 828, 857
NgoMIV GCCGGC 3 cut(s) 122, 453, 483
NlaIII CATG 6 cut(s) 8, 12, 16, 130, 622, 1044
NlaIV GGNNCC 4 cut(s) 177, 289, 895, 1004
NmuCI GTSAC 2 cut(s) 865, 932
NsiI ATGCAT 3 cut(s) 6, 10, 14
NspI RCATGY 2 cut(s) 8, 12
PacI TTAATTAA 1 cut(s) 102
PaeI GCATGC 2 cut(s) 8, 12
PctI GAATGC 2 cut(s) 402, 765
PdiI GCCGGC 3 cut(s) 124, 455, 485
PfeI GAWTC 3 cut(s) 300, 514, 700
PflFI GACNNNGTC 1 cut(s) 818
PflMI CCANNNNNTGG 2 cut(s) 559, 1168
PleI GAGTC 3 cut(s) 148, 194, 416
PpsI GAGTC 3 cut(s) 148, 194, 416
PshBI ATTAAT 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 558, 799, 831, 889
PspFI CCCAGC 2 cut(s) 343, 795
PspGI CCWGG 4 cut(s) 558, 799, 831, 889
PspN4I GGNNCC 4 cut(s) 177, 289, 895, 1004
PspPI GGNCC 4 cut(s) 287, 752, 793, 1130
PstI CTGCAG 1 cut(s) 955
PstNI CAGNNNCTG 5 cut(s) 428, 431, 559, 703, 832
PsuI RGATCY 2 cut(s) 373, 828
PsyI GACNNNGTC 1 cut(s) 818
PvuII CAGCTG 2 cut(s) 146, 428
RsaI GTAC 2 cut(s) 557, 908
RsaNI GTAC 2 cut(s) 556, 907
RseI CAYNNNNRTG 2 cut(s) 891, 902
SaqAI TTAA 6 cut(s) 98, 102, 521, 681, 876, 1125
Sau3AI GATC 5 cut(s) 93, 373, 466, 828, 857
Sau96I GGNCC 4 cut(s) 287, 752, 793, 1130
SchI GAGTC 3 cut(s) 149, 194, 417
ScrFI CCNGG 4 cut(s) 560, 801, 833, 891
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 4 cut(s) 202, 279, 791, 1063
SfcI CTRYAG 1 cut(s) 951
SmiMI CAYNNNNRTG 2 cut(s) 891, 902
SphI GCATGC 2 cut(s) 8, 12
Sse9I AATT 8 cut(s) 64, 99, 253, 337, 412, 496, 747, 993
SsiI CCGC 8 cut(s) 149, 152, 155, 158, 161, 164, 167, 1133
SspMI CTAG 1 cut(s) 740
StyD4I CCNGG 4 cut(s) 558, 799, 831, 889
StyI CCWWGG 1 cut(s) 1006
TaaI ACNGT 1 cut(s) 716
TaqI TCGA 4 cut(s) 273, 441, 501, 569
TasI AATT 8 cut(s) 64, 99, 253, 337, 412, 496, 747, 993
TauI GCSGC 7 cut(s) 152, 155, 158, 161, 164, 167, 170
TfiI GAWTC 3 cut(s) 300, 514, 700
Tru1I TTAA 6 cut(s) 98, 102, 521, 681, 876, 1125
Tru9I TTAA 6 cut(s) 98, 102, 521, 681, 876, 1125
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 2 cut(s) 865, 932
TseI GCWGC 6 cut(s) 146, 425, 428, 837, 953, 1094
Tsp45I GTSAC 2 cut(s) 865, 932
TspDTI ATGAA 1 cut(s) 220
TspRI CASTG 1 cut(s) 973
Tth111I GACNNNGTC 1 cut(s) 818
Van91I CCANNNNNTGG 2 cut(s) 559, 1168
VspI ATTAAT 1 cut(s) 102
XapI RAATTY 2 cut(s) 337, 496
XceI RCATGY 2 cut(s) 8, 12
XcmI CCANNNNNNNNNTGG 1 cut(s) 700
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 1 cut(s) 740
Zsp2I ATGCAT 3 cut(s) 6, 10, 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.