RchiOBHm_Chr5g0000911

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009111
Physical Location & Seq
Forward (+)
497375 .. 498848
1474 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGTTGATTTTGTAGGACAACTTTTGGGTTCAAACAACCTGAATGGGCAAGTTTTGACAAGGAATGGGAACCCTAGACTAGATGCATATGCTGGTTTTCAATCTCATGAAGTGGCTGAGAAGAGTGGAATGTATTCAAATAATAATTGGTATCGAAGTTCAGGAATATTGAATGACATGGGGAACCAATCAAGCTTTGGCAATGACTCGATGCTGGGAAGTAGCTATATAGCTCAAACGGCTATGACTCAATATGGCTCACAGTACTTGCTAGATAGCTTGTTGACTAGGGACCCCGTAGTTACAAATTTTATATTTGAAGGTGTTTTCGATTTCCTATTCAAGGTGATGAATGATTCAAATGGACATCATCTTTTTGGGAAGCTAGTTCAGTCATGCAGCGATAACCAGTTAGGCCTCATTGTAGCAAAGATAACTTTGAATGTCCAATGGCTTATCAACATATCAGTCGGCAGATATGGCTCAAAATCGGTTCAGAGGCTGATCAAGGTGCTTGAGAAATCACCCTTGATTGACATTGTCATAGCAGCCCTATCCTGTGGATTTGAAGAACTGATGACCAATCGCTCAGGATCCTTTGTTATATTGAAGTGCTTAAACCTCCTCGATACTGAGAAAAATCAGAAACTATATGAAGCTGCTGTAAATCTTTGCATCCCATTGGCTCAAAATGAAAAGGGATGTATATACTTGAATGAGTTCATCACGAACAGTAAAGCTCCATATAGAGAGACACTCATGGGTGTGATCTCAAGCAAATCAAAATTCCTTTCTCAGGATCCTTCAGGGAACTTTGTTGTTCAGCACATCCTCGGACTTCACAATCCCATTTACGGTGAGCGCATATGTTTGGAGCTGAGAGAACTTTATATACAGCTCTCGCTACAAAAAGGTGGCAGTCACGTTGTGGAGAAGTGCTTAAACTCATCCGGGATGGTTCATGTGGTAAGTGTTCTTCTCAAGTATGAAAAACTTTGGCAAGTTGCGCGGGATCAATATGGAAACTACGTGATCCAAACAGCATTGAAGACCACAAAACGGGCGAATAGTCCCCTTCATCAAATGCTTATATCGAAGCTCGTACAGAATCGGAATGAACTTGTGATTGGATTTGGAAGGAAAGTGCTTGGTTTGATTGACAATGGGATCCCACTAGACCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

395

Amino Acids

43.99

Weight (kDa)

8.54

Isoelectric Point (pI)

22.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1009
AciI CCGC 1 cut(s) 1009
AclWI GGATC 8 cut(s) 588, 601, 794, 807, 1020, 1027, 1162, 1175
AcsI RAATTY 2 cut(s) 307, 785
AcuI CTGAAG 1 cut(s) 789
AdeI CACNNNGTG 1 cut(s) 928
AfaI GTAC 2 cut(s) 266, 1104
AfiI CCNNNNNNNGG 4 cut(s) 343, 796, 854, 1059
AjuI GAANNNNNNNTTGG 2 cut(s) 1110, 1142
Alw26I GTCTC 1 cut(s) 746
AlwI GGATC 8 cut(s) 588, 601, 794, 807, 1020, 1027, 1162, 1175
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 1 cut(s) 415
ApeKI GCWGC 3 cut(s) 399, 548, 659
ApoI RAATTY 2 cut(s) 307, 785
Asp700I GAANNNNTTC 2 cut(s) 133, 719
AspLEI GCGC 2 cut(s) 864, 1009
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 952
AsuHPI GGTGA 3 cut(s) 358, 516, 869
AvaII GGWCC 1 cut(s) 292
BamHI GGATCC 3 cut(s) 593, 799, 1167
BarI GAAGNNNNNNTAC 2 cut(s) 960, 992
BbsI GAAGAC 1 cut(s) 1055
BbvI GCAGC 3 cut(s) 411, 560, 646
BccI CCATC 1 cut(s) 949
BceAI ACGGC 1 cut(s) 255
BclI TGATCA 1 cut(s) 504
BcnI CCSGG 1 cut(s) 952
BcoDI GTCTC 1 cut(s) 746
BfaI CTAG 6 cut(s) 75, 80, 272, 288, 386, 1175
BisI GCNGC 3 cut(s) 400, 549, 660
BlsI GCNGC 3 cut(s) 401, 550, 661
BmcAI AGTACT 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 952
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 7 cut(s) 71, 185, 293, 294, 595, 801, 1169
BmrFI CCNGG 1 cut(s) 952
BmsI GCATC 3 cut(s) 73, 201, 684
BpiI GAAGAC 1 cut(s) 1055
BplI GAGNNNNNCTC 2 cut(s) 741, 773
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 3 cut(s) 536, 757, 965
BpuMI CCSGG 1 cut(s) 952
BsaAI YACGTR 1 cut(s) 1030
BsaJI CCNNGG 1 cut(s) 832
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 796, 854, 1059
Bse1I ACTGG 1 cut(s) 409
Bse3DI GCAATG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 832
BseGI GGATG 5 cut(s) 675, 707, 828, 947, 960
BseLI CCNNNNNNNGG 4 cut(s) 343, 796, 854, 1059
BseMI GCAATG 1 cut(s) 208
BseMII CTCAG 5 cut(s) 108, 603, 624, 809, 869
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 3 cut(s) 411, 560, 646
BseYI CCCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 1009
BshFI GGCC 1 cut(s) 417
BsiSI CCGG 1 cut(s) 951
BslFI GGGAC 2 cut(s) 305, 1056
BslI CCNNNNNNNGG 4 cut(s) 343, 796, 854, 1059
BsmAI GTCTC 1 cut(s) 746
BsmFI GGGAC 2 cut(s) 305, 1056
BsnI GGCC 1 cut(s) 417
Bsp143I GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
BspACI CCGC 1 cut(s) 1009
BspANI GGCC 1 cut(s) 417
BspCNI CTCAG 5 cut(s) 109, 602, 625, 808, 870
BspFNI CGCG 1 cut(s) 1009
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 7 cut(s) 71, 185, 293, 294, 595, 801, 1169
BspPI GGATC 8 cut(s) 588, 601, 794, 807, 1020, 1027, 1162, 1175
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 832
BssMI GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
Bst4CI ACNGT 3 cut(s) 264, 734, 857
Bst6I CTCTTC 1 cut(s) 116
BstBAI YACGTR 1 cut(s) 1030
BstDEI CTNAG 5 cut(s) 117, 589, 633, 795, 878
BstENI CCTNNNNNAGG 2 cut(s) 341, 794
BstF5I GGATG 5 cut(s) 675, 707, 828, 947, 960
BstFNI CGCG 1 cut(s) 1009
BstHHI GCGC 2 cut(s) 864, 1009
BstKTI GATC 7 cut(s) 507, 596, 771, 802, 1015, 1035, 1170
BstMAI GTCTC 1 cut(s) 746
BstMBI GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
BstMWI GCNNNNNNNGC 3 cut(s) 239, 480, 1006
BstSCI CCNGG 1 cut(s) 950
BstUI CGCG 1 cut(s) 1009
BstV1I GCAGC 3 cut(s) 411, 560, 646
BstV2I GAAGAC 1 cut(s) 1055
BstX2I RGATCY 3 cut(s) 593, 799, 1167
BstYI RGATCY 3 cut(s) 593, 799, 1167
BsuRI GGCC 1 cut(s) 417
BtsCI GGATG 5 cut(s) 675, 707, 828, 947, 960
CaiI CAGNNNCTG 1 cut(s) 502
CciI TCATGA 1 cut(s) 106
CfoI GCGC 2 cut(s) 864, 1009
Cfr13I GGNCC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 265, 1103
CviAII CATG 5 cut(s) 107, 178, 396, 760, 962
CviQI GTAC 2 cut(s) 265, 1103
DdeI CTNAG 5 cut(s) 117, 589, 633, 795, 878
DpnI GATC 7 cut(s) 506, 595, 770, 801, 1014, 1034, 1169
DpnII GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
DraIII CACNNNGTG 1 cut(s) 928
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco147I AGGCCT 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 789
EcoNI CCTNNNNNAGG 2 cut(s) 341, 794
EcoO109I RGGNCCY 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 88
FaeI CATG 5 cut(s) 110, 181, 399, 763, 965
FaqI GGGAC 2 cut(s) 305, 1056
FatI CATG 5 cut(s) 106, 177, 395, 759, 961
FauI CCCGC 1 cut(s) 1002
FauNDI CATATG 2 cut(s) 88, 866
FbaI TGATCA 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 400, 549, 660
FokI GGATG 5 cut(s) 662, 714, 815, 934, 967
Fsp4HI GCNGC 3 cut(s) 400, 549, 660
FspBI CTAG 6 cut(s) 75, 80, 272, 288, 386, 1175
GlaI GCGC 2 cut(s) 863, 1008
GluI GCNGC 3 cut(s) 400, 549, 660
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 1 cut(s) 417
HapII CCGG 1 cut(s) 951
HhaI GCGC 2 cut(s) 864, 1009
Hin1II CATG 5 cut(s) 110, 181, 399, 763, 965
Hin6I GCGC 2 cut(s) 862, 1007
HinP1I GCGC 2 cut(s) 862, 1007
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 4 cut(s) 206, 247, 356, 1108
HpaII CCGG 1 cut(s) 951
HphI GGTGA 3 cut(s) 358, 516, 869
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 4 cut(s) 498, 645, 836, 1113
Hpy188III TCNNGA 5 cut(s) 107, 162, 591, 727, 797
Hpy8I GTNNAC 1 cut(s) 285
HpyAV CCTTC 4 cut(s) 314, 813, 1085, 1131
HpyCH4III ACNGT 3 cut(s) 264, 734, 857
HpyCH4IV ACGT 2 cut(s) 924, 1029
HpyCH4V TGCA 3 cut(s) 86, 399, 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 480, 1006
HpyF3I CTNAG 5 cut(s) 117, 589, 633, 795, 878
HpySE526I ACGT 2 cut(s) 924, 1029
Hsp92II CATG 5 cut(s) 110, 181, 399, 763, 965
HspAI GCGC 2 cut(s) 862, 1007
KflI GGGWCCC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
LmnI GCTCC 2 cut(s) 745, 874
Lsp1109I GCAGC 3 cut(s) 411, 560, 646
LweI GCATC 3 cut(s) 73, 201, 684
MaeI CTAG 6 cut(s) 75, 80, 272, 288, 386, 1175
MaeII ACGT 2 cut(s) 924, 1029
MaeIII GTNAC 2 cut(s) 301, 920
MalI GATC 7 cut(s) 506, 595, 770, 801, 1014, 1034, 1169
MboI GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
MboII GAAGA 4 cut(s) 133, 581, 968, 1060
MflI RGATCY 3 cut(s) 593, 799, 1167
MluCI AATT 3 cut(s) 145, 307, 785
MlyI GAGTC 2 cut(s) 200, 241
MnlI CCTC 5 cut(s) 428, 492, 632, 635, 842
Mph1103I ATGCAT 1 cut(s) 88
MroXI GAANNNNTTC 2 cut(s) 133, 719
MseI TTAA 2 cut(s) 617, 941
MslI CAYNNNNRTG 1 cut(s) 764
MspI CCGG 1 cut(s) 951
MspR9I CCNGG 1 cut(s) 952
MvnI CGCG 1 cut(s) 1009
MwoI GCNNNNNNNGC 3 cut(s) 239, 480, 1006
NciI CCSGG 1 cut(s) 952
NdeI CATATG 2 cut(s) 88, 866
NdeII GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
NlaIII CATG 5 cut(s) 110, 181, 399, 763, 965
NlaIV GGNNCC 7 cut(s) 71, 185, 293, 294, 595, 801, 1169
NmuCI GTSAC 1 cut(s) 920
NsiI ATGCAT 1 cut(s) 88
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 417
PdmI GAANNNNTTC 2 cut(s) 133, 719
PfeI GAWTC 2 cut(s) 356, 1108
PflFI GACNNNGTC 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 950
PkrI GCNGC 3 cut(s) 401, 550, 661
PleI GAGTC 2 cut(s) 200, 241
PpsI GAGTC 2 cut(s) 200, 241
Ppu21I YACGTR 1 cut(s) 1030
PpuMI RGGWCCY 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 7 cut(s) 71, 185, 293, 294, 595, 801, 1169
PspPI GGNCC 1 cut(s) 292
PspPPI RGGWCCY 1 cut(s) 292
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 3 cut(s) 593, 799, 1167
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 2 cut(s) 266, 1104
RsaNI GTAC 2 cut(s) 265, 1103
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 2 cut(s) 617, 941
SatI GCNGC 3 cut(s) 400, 549, 660
Sau3AI GATC 7 cut(s) 504, 593, 768, 799, 1012, 1032, 1167
Sau96I GGNCC 1 cut(s) 292
ScaI AGTACT 1 cut(s) 266
SchI GAGTC 2 cut(s) 200, 241
ScrFI CCNGG 1 cut(s) 952
SfaNI GCATC 3 cut(s) 73, 201, 684
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 3 cut(s) 515, 772, 980
SmoI CTYRAG 3 cut(s) 515, 772, 980
Sse9I AATT 3 cut(s) 145, 307, 785
SseBI AGGCCT 1 cut(s) 417
SsiI CCGC 1 cut(s) 1009
SspI AATATT 1 cut(s) 168
SspMI CTAG 6 cut(s) 75, 80, 272, 288, 386, 1175
StuI AGGCCT 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 950
TaaI ACNGT 3 cut(s) 264, 734, 857
TaiI ACGT 2 cut(s) 927, 1032
TaqI TCGA 5 cut(s) 154, 209, 330, 627, 1094
TasI AATT 3 cut(s) 145, 307, 785
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 2 cut(s) 356, 1108
Tru1I TTAA 2 cut(s) 617, 941
Tru9I TTAA 2 cut(s) 617, 941
TseFI GTSAC 1 cut(s) 920
TseI GCWGC 3 cut(s) 399, 548, 659
Tsp45I GTSAC 1 cut(s) 920
TspDTI ATGAA 9 cut(s) 123, 365, 669, 708, 712, 950, 1002, 1067, 1131
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 292
XagI CCTNNNNNAGG 2 cut(s) 341, 794
XapI RAATTY 2 cut(s) 307, 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmnI GAANNNNTTC 2 cut(s) 133, 719
XspI CTAG 6 cut(s) 75, 80, 272, 288, 386, 1175
ZrmI AGTACT 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.