RchiOBHm_Chr5g0013641

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012572
Physical Location & Seq
Forward (+)
9189891 .. 9192079
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGTCTAAAGAGCAAGAGCTCGAGTATGCTATGCTTTTTGCTAAGTGGAAAGAGGCCGAACCTATTACCAATCGCGTGATGGCCTCCATCCCGGAGCCTCTTTTTGGTGGACGCATTAACGTAGTACAGAGTTATGGTCCTGATACGCTAGCTGCAATGGATTCTCGAAGACAAAGAAGGGCAGAAAACCCAAAGTATTATGGAGAAGCAGCATTGAACGAATACATCGAACGTCAAAAGAGTACATGGGGGCCGTGTGAGATTTGTTTTAAGGCTGGTGACCATATTAGTTTGTACTGCCCATATCGTGACTATGTCCCCGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTCGGAATTGTGGACTTCCGGACCAGCACCCTGGTGCTAAACGTGCTATTCGCAAGTCGTGTTTCATTTGTGCATCAGACCACTGGGGTACTGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCTTTCCCTTCAGCGACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

19.38

Weight (kDa)

8.09

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 75, 460
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 2 cut(s) 458, 511
AcuI CTGAAG 1 cut(s) 487
AfaI GTAC 4 cut(s) 126, 244, 296, 448
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 2 cut(s) 217, 350
AhlI ACTAGT 1 cut(s) 352
AjnI CCWGG 2 cut(s) 340, 388
AleI CACNNNNGTG 1 cut(s) 390
AluBI AGCT 2 cut(s) 19, 152
AluI AGCT 2 cut(s) 19, 152
Alw21I GWGCWC 1 cut(s) 21
Ama87I CYCGRG 1 cut(s) 20
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 4 cut(s) 54, 81, 251, 337
ApeKI GCWGC 2 cut(s) 152, 209
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 4 cut(s) 137, 251, 338, 379
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 290
AsuNHI GCTAGC 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 2 cut(s) 137, 379
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 139, 221
BccI CCATC 2 cut(s) 73, 95
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 2 cut(s) 342, 390
BcnI CCSGG 1 cut(s) 92
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 2 cut(s) 149, 353
BisI GCNGC 2 cut(s) 153, 210
BlsI GCNGC 2 cut(s) 154, 211
Bme1390I CCNGG 3 cut(s) 92, 342, 390
Bme18I GGWCC 2 cut(s) 137, 379
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 4 cut(s) 137, 251, 338, 379
BmiI GGNNCC 3 cut(s) 96, 252, 483
BmrFI CCNGG 3 cut(s) 92, 342, 390
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 338, 440
BmtI GCTAGC 1 cut(s) 152
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 340, 388
BsaWI WCCGGW 1 cut(s) 376
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 162
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 2 cut(s) 342, 390
BseDI CCNNGG 2 cut(s) 340, 388
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 446
BseXI GCAGC 2 cut(s) 139, 221
Bsh1236I CGCG 2 cut(s) 75, 460
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 4 cut(s) 56, 83, 253, 339
BsiEI CGRYCG 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 92, 377
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 4 cut(s) 56, 83, 253, 339
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 21
Bsp13I TCCGGA 1 cut(s) 376
BspACI CCGC 2 cut(s) 458, 511
BspANI GGCC 4 cut(s) 56, 83, 253, 339
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 2 cut(s) 75, 460
BspLI GGNNCC 3 cut(s) 96, 252, 483
BspOI GCTAGC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 340, 388
Bst2UI CCWGG 2 cut(s) 342, 390
Bst4CI ACNGT 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 150, 458, 462
BstDEI CTNAG 1 cut(s) 42
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 1 cut(s) 87
BstFNI CGCG 2 cut(s) 75, 460
BstHHI GCGC 1 cut(s) 329
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 2 cut(s) 342, 390
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 3 cut(s) 90, 340, 388
BstUI CGCG 2 cut(s) 75, 460
BstV1I GCAGC 2 cut(s) 139, 221
BstV2I GAAGAC 1 cut(s) 175
BstXI CCANNNNNNTGG 1 cut(s) 389
BsuRI GGCC 4 cut(s) 56, 83, 253, 339
BtsCI GGATG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 3 cut(s) 150, 458, 462
CfoI GCGC 1 cut(s) 329
Cfr13I GGNCC 4 cut(s) 137, 251, 338, 379
CseI GACGC 1 cut(s) 120
Csp6I GTAC 4 cut(s) 125, 243, 295, 447
CviAII CATG 1 cut(s) 246
CviJI RGCY 8 cut(s) 19, 56, 83, 97, 152, 253, 275, 339
CviKI_1 RGCY 8 cut(s) 19, 56, 83, 97, 152, 253, 275, 339
CviQI GTAC 4 cut(s) 125, 243, 295, 447
DdeI CTNAG 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 137, 379
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 487
Eco88I CYCGRG 1 cut(s) 20
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 19
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 340, 388
EcoT38I GRGCYC 1 cut(s) 21
FaeI CATG 1 cut(s) 249
FaqI GGGAC 1 cut(s) 302
FatI CATG 1 cut(s) 245
FauI CCCGC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 153, 210
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 2 cut(s) 153, 210
FspBI CTAG 2 cut(s) 149, 353
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 153, 210
HaeIII GGCC 4 cut(s) 56, 83, 253, 339
HapII CCGG 2 cut(s) 92, 377
HgaI GACGC 1 cut(s) 120
HhaI GCGC 1 cut(s) 329
Hin1II CATG 1 cut(s) 249
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 92, 377
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 110, 371
Hpy188I TCNGA 2 cut(s) 363, 436
Hpy188III TCNNGA 4 cut(s) 140, 165, 308, 377
Hpy8I GTNNAC 2 cut(s) 110, 371
HpyAV CCTTC 3 cut(s) 171, 317, 511
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 3 cut(s) 120, 232, 400
HpyCH4V TGCA 2 cut(s) 155, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 3 cut(s) 120, 232, 400
Hsp92II CATG 1 cut(s) 249
HspAI GCGC 1 cut(s) 327
Kpn2I TCCGGA 1 cut(s) 376
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 2 cut(s) 139, 221
LweI GCATC 2 cut(s) 338, 440
MaeI CTAG 2 cut(s) 149, 353
MaeII ACGT 3 cut(s) 120, 232, 400
MaeIII GTNAC 2 cut(s) 278, 308
MboII GAAGA 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 3 cut(s) 46, 94, 108
MroI TCCGGA 1 cut(s) 376
MseI TTAA 2 cut(s) 117, 270
MslI CAYNNNNRTG 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 513
MspI CCGG 2 cut(s) 92, 377
MspR9I CCNGG 3 cut(s) 92, 342, 390
MvaI CCWGG 2 cut(s) 342, 390
MvnI CGCG 2 cut(s) 75, 460
MwoI GCNNNNNNNGC 1 cut(s) 401
NciI CCSGG 1 cut(s) 92
NheI GCTAGC 1 cut(s) 148
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 3 cut(s) 96, 252, 483
NmuCI GTSAC 2 cut(s) 278, 308
OliI CACNNNNGTG 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 1 cut(s) 161
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 2 cut(s) 154, 211
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 340, 388
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 2 cut(s) 340, 388
PspN4I GGNNCC 3 cut(s) 96, 252, 483
PspPI GGNCC 4 cut(s) 137, 251, 338, 379
PspXI VCTCGAGB 1 cut(s) 20
PsyI GACNNNGTC 1 cut(s) 314
RsaI GTAC 4 cut(s) 126, 244, 296, 448
RsaNI GTAC 4 cut(s) 125, 243, 295, 447
RseI CAYNNNNRTG 1 cut(s) 390
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 117, 270
SatI GCNGC 2 cut(s) 153, 210
Sau96I GGNCC 4 cut(s) 137, 251, 338, 379
ScrFI CCNGG 3 cut(s) 92, 342, 390
SduI GDGCHC 1 cut(s) 21
SetI ASST 6 cut(s) 21, 64, 123, 154, 235, 403
SfaNI GCATC 2 cut(s) 338, 440
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 2 cut(s) 137, 379
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SpeI ACTAGT 1 cut(s) 352
Sse9I AATT 1 cut(s) 364
SsiI CCGC 2 cut(s) 458, 511
SspMI CTAG 2 cut(s) 149, 353
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 3 cut(s) 90, 340, 388
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 3 cut(s) 123, 235, 403
TaqI TCGA 3 cut(s) 21, 166, 228
TasI AATT 1 cut(s) 364
TatI WGTACW 3 cut(s) 124, 242, 294
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 117, 270
Tru9I TTAA 2 cut(s) 117, 270
TscAI CASTG 1 cut(s) 446
TseFI GTSAC 2 cut(s) 278, 308
TseI GCWGC 2 cut(s) 152, 209
Tsp45I GTSAC 2 cut(s) 278, 308
TspDTI ATGAA 1 cut(s) 412
TspRI CASTG 1 cut(s) 446
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 137, 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 2 cut(s) 149, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.