Rh5BG102400

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012572
Physical Location & Seq
Forward (+)
9312246 .. 9326223
13978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG102400.1

Sequence Viewer

Length: 834 bp
ATGCCCCTATCCCCTATCGTGGTCCGCCTGAAGCGGCACCGTTCGTCCTTGTTCGAAGACCCTAGCCACGACCAGAACCTAACCCCTGTCACGGAAGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTGTCCTCTGAACACCCTGATATCCAGGGCGGCAAGCACCAGGACCCGGACCCGGACCCGGCGACGCCCTTAAAACCAGTAAGCCGTACGCTGAAGGAGATTATGGATGAGGCCGGCCCGCTCATCTCTAAAGAGCTACAGCTCGAGTATGCTAAGATTTTTGCTAAGTCGAAGGAGGCCGAACCTATTACCAATCGCGTGACAGGGTTTGGTGGACCCATTAACGTAGTAGAGAGTTATGAGGCCGAACCTATTACCAATCGCGTGATGGCCTCCTTCCCGGAGCCTCTTTTTGGTGGACGCATTAACGTAGTACAGAGTTATGGTCCTGATACGCTAGCTGCAATGGAGTCTCTGAGACGTAGAAGGGCAGAAAACCCAAAGTATTGTGGAGAAGCAGCATTGAACGAATACATCGAACGTCAAAAGAGTACATGGGGGCCGTGTGAGATTTGTTTTAAGGCTGGTGACCATATTAGTTTGTACTGCCCATATCGTGACTATGTCCCGGAAGGCGCATCTGTTGGCCCTGGCGATGAACTAGTTTGTAGGAATTGTGGACTTGCGGACCAGCACCCTGGTGCTAAACGTGCTATTCTCAAGTCGTGTTTCATTTGTGCTTCGGACCACTGGGGTACCGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCTTTCCCTTCAGCGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

30.78

Weight (kDa)

6.26

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 36, 764
AccBSI CCGCTC 1 cut(s) 250
AccII CGCG 3 cut(s) 327, 393, 778
AciI CCGC 8 cut(s) 25, 34, 115, 159, 248, 695, 776, 829
AcuI CTGAAG 3 cut(s) 50, 242, 805
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 5 cut(s) 217, 444, 562, 614, 766
AfiI CCNNNNNNNGG 6 cut(s) 19, 91, 175, 181, 187, 422
AgsI TTSAA 1 cut(s) 535
AhlI ACTAGT 1 cut(s) 670
AjnI CCWGG 4 cut(s) 153, 168, 658, 706
AleI CACNNNNGTG 1 cut(s) 708
AluBI AGCT 3 cut(s) 265, 271, 470
AluI AGCT 3 cut(s) 265, 271, 470
Alw26I GTCTC 2 cut(s) 481, 486
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 8 cut(s) 116, 240, 244, 306, 372, 399, 569, 655
ApeKI GCWGC 2 cut(s) 470, 527
Asp718I GGTACC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 647
AsuC2I CCSGG 5 cut(s) 176, 182, 188, 410, 638
AsuHPI GGTGA 1 cut(s) 608
AsuII TTCGAA 1 cut(s) 54
AsuNHI GCTAGC 1 cut(s) 466
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 8 cut(s) 22, 172, 178, 184, 344, 455, 697, 754
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BanI GGYRCC 2 cut(s) 36, 764
BbsI GAAGAC 2 cut(s) 63, 102
BbvI GCAGC 2 cut(s) 457, 539
BccI CCATC 1 cut(s) 391
BceAI ACGGC 2 cut(s) 198, 556
BciT130I CCWGG 4 cut(s) 155, 170, 660, 708
BcnI CCSGG 5 cut(s) 176, 182, 188, 410, 638
BcoDI GTCTC 2 cut(s) 481, 486
BcuI ACTAGT 1 cut(s) 670
BfaI CTAG 3 cut(s) 63, 467, 671
BfmI CTRYAG 1 cut(s) 266
BisI GCNGC 5 cut(s) 35, 116, 160, 471, 528
BlsI GCNGC 5 cut(s) 36, 117, 161, 472, 529
Bme1390I CCNGG 9 cut(s) 155, 170, 176, 182, 188, 410, 638, 660, 708
Bme18I GGWCC 8 cut(s) 22, 172, 178, 184, 344, 455, 697, 754
BmeT110I CYCGRG 1 cut(s) 272
BmrFI CCNGG 9 cut(s) 155, 170, 176, 182, 188, 410, 638, 660, 708
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 1 cut(s) 656
BmtI GCTAGC 1 cut(s) 470
BmuI ACTGGG 1 cut(s) 769
BpiI GAAGAC 2 cut(s) 63, 102
Bpu14I TTCGAA 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 5 cut(s) 176, 182, 188, 410, 638
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 154, 658, 706
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 91, 175, 181, 187, 422
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 2 cut(s) 206, 764
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 4 cut(s) 155, 170, 660, 708
BseDI CCNNGG 3 cut(s) 154, 658, 706
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 6 cut(s) 19, 91, 175, 181, 187, 422
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 206, 764
BseXI GCAGC 2 cut(s) 457, 539
Bsh1236I CGCG 3 cut(s) 327, 393, 778
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 8 cut(s) 118, 242, 246, 308, 374, 401, 571, 657
BshNI GGYRCC 2 cut(s) 36, 764
BsiEI CGRYCG 1 cut(s) 829
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 6 cut(s) 176, 182, 188, 243, 410, 638
BsiWI CGTACG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 6 cut(s) 19, 91, 175, 181, 187, 422
BsmAI GTCTC 2 cut(s) 481, 486
BsmBI CGTCTC 1 cut(s) 481
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 8 cut(s) 118, 242, 246, 308, 374, 401, 571, 657
BsoBI CYCGRG 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 54
BspACI CCGC 8 cut(s) 25, 34, 115, 159, 248, 695, 776, 829
BspANI GGCC 8 cut(s) 118, 242, 246, 308, 374, 401, 571, 657
BspCNI CTCAG 1 cut(s) 477
BspFNI CGCG 3 cut(s) 327, 393, 778
BspOI GCTAGC 1 cut(s) 470
BspT104I TTCGAA 1 cut(s) 54
BspT107I GGYRCC 2 cut(s) 36, 764
BsrBI CCGCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 2 cut(s) 206, 764
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 154, 658, 706
BssNI GRCGYC 1 cut(s) 194
Bst2UI CCWGG 4 cut(s) 155, 170, 660, 708
Bst4CI ACNGT 2 cut(s) 41, 799
BstACI GRCGYC 1 cut(s) 194
BstBI TTCGAA 1 cut(s) 54
BstC8I GCNNGC 6 cut(s) 164, 244, 248, 468, 776, 780
BstDEI CTNAG 3 cut(s) 282, 294, 485
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 1 cut(s) 241
BstFNI CGCG 3 cut(s) 327, 393, 778
BstHHI GCGC 1 cut(s) 647
BstMAI GTCTC 2 cut(s) 481, 486
BstMCI CGRYCG 1 cut(s) 829
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNI CCWGG 4 cut(s) 155, 170, 660, 708
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 9 cut(s) 153, 168, 174, 180, 186, 408, 636, 658, 706
BstSFI CTRYAG 1 cut(s) 266
BstUI CGCG 3 cut(s) 327, 393, 778
BstV1I GCAGC 2 cut(s) 457, 539
BstV2I GAAGAC 2 cut(s) 63, 102
BstXI CCANNNNNNTGG 1 cut(s) 707
BsuRI GGCC 8 cut(s) 118, 242, 246, 308, 374, 401, 571, 657
BtgZI GCGATG 1 cut(s) 678
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 757
Cac8I GCNNGC 6 cut(s) 164, 244, 248, 468, 776, 780
CfoI GCGC 1 cut(s) 647
Cfr10I RCCGGY 1 cut(s) 242
CseI GACGC 2 cut(s) 202, 438
Csp6I GTAC 5 cut(s) 216, 443, 561, 613, 765
CviAII CATG 1 cut(s) 564
CviQI GTAC 5 cut(s) 216, 443, 561, 613, 765
DdeI CTNAG 3 cut(s) 282, 294, 485
EciI GGCGGA 1 cut(s) 14
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 8 cut(s) 22, 172, 178, 184, 344, 455, 697, 754
Eco57I CTGAAG 3 cut(s) 50, 242, 805
Eco88I CYCGRG 1 cut(s) 272
Eco91I GGTNACC 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 172
EcoO65I GGTNACC 1 cut(s) 596
EcoRII CCWGG 4 cut(s) 153, 168, 658, 706
EcoRV GATATC 1 cut(s) 151
Esp3I CGTCTC 1 cut(s) 481
FaeI CATG 1 cut(s) 567
FaqI GGGAC 1 cut(s) 620
FatI CATG 1 cut(s) 563
FauI CCCGC 2 cut(s) 255, 783
Fnu4HI GCNGC 5 cut(s) 35, 116, 160, 471, 528
FokI GGATG 1 cut(s) 248
FseI GGCCGGCC 1 cut(s) 246
Fsp4HI GCNGC 5 cut(s) 35, 116, 160, 471, 528
FspBI CTAG 3 cut(s) 63, 467, 671
GlaI GCGC 1 cut(s) 646
GluI GCNGC 5 cut(s) 35, 116, 160, 471, 528
HaeIII GGCC 8 cut(s) 118, 242, 246, 308, 374, 401, 571, 657
HapII CCGG 6 cut(s) 176, 182, 188, 243, 410, 638
HgaI GACGC 2 cut(s) 202, 438
HhaI GCGC 1 cut(s) 647
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 1 cut(s) 567
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HinfI GANTC 2 cut(s) 98, 479
HpaII CCGG 6 cut(s) 176, 182, 188, 243, 410, 638
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 3 cut(s) 344, 428, 689
Hpy188I TCNGA 4 cut(s) 103, 139, 486, 754
Hpy188III TCNNGA 2 cut(s) 458, 626
Hpy8I GTNNAC 3 cut(s) 344, 428, 689
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 6 cut(s) 217, 295, 415, 489, 635, 829
HpyCH4III ACNGT 2 cut(s) 41, 799
HpyCH4IV ACGT 5 cut(s) 354, 438, 490, 550, 718
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 3 cut(s) 282, 294, 485
HpySE526I ACGT 5 cut(s) 354, 438, 490, 550, 718
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 1 cut(s) 567
HspAI GCGC 1 cut(s) 645
KpnI GGTACC 1 cut(s) 768
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 2 cut(s) 457, 539
LweI GCATC 1 cut(s) 656
MaeI CTAG 3 cut(s) 63, 467, 671
MaeII ACGT 5 cut(s) 354, 438, 490, 550, 718
MaeIII GTNAC 4 cut(s) 88, 328, 596, 626
MbiI CCGCTC 1 cut(s) 250
MboII GAAGA 2 cut(s) 68, 107
MluCI AATT 1 cut(s) 682
MlyI GAGTC 2 cut(s) 92, 488
MnlI CCTC 6 cut(s) 145, 232, 298, 364, 412, 426
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 4 cut(s) 200, 351, 435, 588
MslI CAYNNNNRTG 1 cut(s) 708
MspI CCGG 6 cut(s) 176, 182, 188, 243, 410, 638
MspR9I CCNGG 9 cut(s) 155, 170, 176, 182, 188, 410, 638, 660, 708
MvaI CCWGG 4 cut(s) 155, 170, 660, 708
MvnI CGCG 3 cut(s) 327, 393, 778
MwoI GCNNNNNNNGC 1 cut(s) 719
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 5 cut(s) 176, 182, 188, 410, 638
NgoMIV GCCGGC 1 cut(s) 242
NheI GCTAGC 1 cut(s) 466
NlaIII CATG 1 cut(s) 567
NmuCI GTSAC 4 cut(s) 88, 328, 596, 626
NspV TTCGAA 1 cut(s) 54
OliI CACNNNNGTG 1 cut(s) 708
PaeR7I CTCGAG 1 cut(s) 272
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdiI GCCGGC 1 cut(s) 244
Pfl23II CGTACG 1 cut(s) 215
PflFI GACNNNGTC 1 cut(s) 632
PfoI TCCNGGA 2 cut(s) 408, 636
PkrI GCNGC 5 cut(s) 36, 117, 161, 472, 529
PleI GAGTC 2 cut(s) 92, 487
PpsI GAGTC 2 cut(s) 92, 487
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 4 cut(s) 153, 168, 658, 706
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 4 cut(s) 153, 168, 658, 706
PspLI CGTACG 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 172
PspXI VCTCGAGB 1 cut(s) 272
PsyI GACNNNGTC 1 cut(s) 632
RigI GGCCGGCC 1 cut(s) 246
RsaI GTAC 5 cut(s) 217, 444, 562, 614, 766
RsaNI GTAC 5 cut(s) 216, 443, 561, 613, 765
RseI CAYNNNNRTG 1 cut(s) 708
SaqAI TTAA 4 cut(s) 200, 351, 435, 588
SatI GCNGC 5 cut(s) 35, 116, 160, 471, 528
SchI GAGTC 2 cut(s) 92, 488
ScrFI CCNGG 9 cut(s) 155, 170, 176, 182, 188, 410, 638, 660, 708
SfaNI GCATC 1 cut(s) 656
SfcI CTRYAG 1 cut(s) 266
Sfr274I CTCGAG 1 cut(s) 272
SfuI TTCGAA 1 cut(s) 54
SinI GGWCC 8 cut(s) 22, 172, 178, 184, 344, 455, 697, 754
SlaI CTCGAG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 2 cut(s) 272, 728
SmoI CTYRAG 2 cut(s) 272, 728
SpeI ACTAGT 1 cut(s) 670
Sse9I AATT 1 cut(s) 682
SsiI CCGC 8 cut(s) 25, 34, 115, 159, 248, 695, 776, 829
SspMI CTAG 3 cut(s) 63, 467, 671
StyD4I CCNGG 9 cut(s) 153, 168, 174, 180, 186, 408, 636, 658, 706
TaaI ACNGT 2 cut(s) 41, 799
TaiI ACGT 5 cut(s) 357, 441, 493, 553, 721
TaqI TCGA 4 cut(s) 54, 273, 299, 546
TasI AATT 1 cut(s) 682
TatI WGTACW 3 cut(s) 442, 560, 612
TauI GCSGC 3 cut(s) 37, 118, 162
Tru1I TTAA 4 cut(s) 200, 351, 435, 588
Tru9I TTAA 4 cut(s) 200, 351, 435, 588
TscAI CASTG 1 cut(s) 764
TseFI GTSAC 4 cut(s) 88, 328, 596, 626
TseI GCWGC 2 cut(s) 470, 527
Tsp45I GTSAC 4 cut(s) 88, 328, 596, 626
TspDTI ATGAA 2 cut(s) 681, 730
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 1 cut(s) 764
Tth111I GACNNNGTC 1 cut(s) 632
VpaK11BI GGWCC 8 cut(s) 22, 172, 178, 184, 344, 455, 697, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XhoI CTCGAG 1 cut(s) 272
XspI CTAG 3 cut(s) 63, 467, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.