Rh5DG100900

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012572
Physical Location & Seq
Forward (+)
9218753 .. 9221162
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG100900.1

Sequence Viewer

Length: 1065 bp
ATGCCCCTATCCCCTGATGTCCAGGGCGACCAAGGCCAGATCCTGGTCCGCCTGAAGCGGCCCCATTCGTCCTTGTCGGAAGACCCTAGCCAGGACCAGAACCTAACCCTAACCCCTGTCACGGCTGAAGACCCTGATGTCCAGGGCGGCCAGCACCAGGAGCCGGACCCGGTCCAGGTCCAGCCGGCGAAGCCCTCTGGAGACCCTGATGTTCCGGGCGGCGAGCACCACGACCCGGACCCGGCCATGCCGCTATCCCCTATCGAGGTCCGCCTGAAGCGGAACAATTCGGCCATGCCCCTATCCCCTATCGTGGTCCGCCTGAAGCGGCACCATTCGTCCTTGTTCGAAGACCGTATACATGACCACAACCTAACCCCTGTCACGGAAGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTGTCCTCTGAAGACCCTGATGTCCAGGGCGGCCAGCACCAGAACCCGGACCCGGTCCAGCCCGTGAAGCCCTCAGAACCAGTAAGCCGTACGCTGAAGGAGATTCTCGATGAGTGCGGCCCGAACATGTCTAAAGAGCAAGAGCTCGAGTATGCTATGCTTTTTGCTAAGTGGAAAGAGGCCGAACCTATTACCAATCGCGTGATGGCCTCCATCCCAGAGCCTCTTTTTGGTGGGCGCATTAACGTAGTACAGAGTTATGGTCCTGATACGCTAGCTGCAATGGATTCTCGAAGACAAAGAAGGGCAGAAAACCCAAAGTATTATGGAGAAGCAGCATTGAACGAATACATCGAACGTCAAAAGAGTACATGGGGGCCGTGTGAGATTTGTTTTAAGGCTGGTGACCATATTAGTTTGTACTGCCCATATCGTGACTATGTCCCGGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTCGGAATTGTGGACTTCCGGACCAGCACCCTGGTGCTAAACGTGCTATTCGCAAGTCGTGTTTCATTTGTGCATCAGACCACTGGGGTACCGATTGCCCGCGCGCCGAACAGCATAAAACGGTTCCATATAATCCCTTTCCCTTCAGCGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

354

Amino Acids

39.37

Weight (kDa)

5.48

Isoelectric Point (pI)

61.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 443
Acc65I GGTACC 1 cut(s) 995
AccB1I GGYRCC 2 cut(s) 330, 995
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 1 cut(s) 358
AccII CGCG 3 cut(s) 624, 1009, 1011
AccIII TCCGGA 1 cut(s) 925
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 4 cut(s) 148, 243, 291, 454
AcuI CTGAAG 7 cut(s) 74, 147, 296, 344, 453, 539, 1036
AfaI GTAC 5 cut(s) 514, 675, 793, 845, 997
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 2 cut(s) 766, 899
AhlI ACTAGT 1 cut(s) 901
AjnI CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 447, 889, 937
AleI CACNNNNGTG 1 cut(s) 939
AluBI AGCT 2 cut(s) 568, 701
AluI AGCT 2 cut(s) 568, 701
Alw21I GWGCWC 2 cut(s) 228, 570
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 34
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 569
Aor13HI TCCGGA 1 cut(s) 925
ApeKI GCWGC 2 cut(s) 701, 758
Asp718I GGTACC 1 cut(s) 995
AspLEI GCGC 4 cut(s) 663, 878, 1011, 1013
AsuC2I CCSGG 7 cut(s) 170, 216, 236, 242, 470, 476, 869
AsuHPI GGTGA 1 cut(s) 839
AsuII TTCGAA 1 cut(s) 348
AsuNHI GCTAGC 1 cut(s) 697
AvaI CYCGRG 1 cut(s) 569
BaeI ACNNNNGTAYC 2 cut(s) 979, 1012
BanI GGYRCC 2 cut(s) 330, 995
BanII GRGCYC 1 cut(s) 570
BarI GAAGNNNNNNTAC 2 cut(s) 342, 374
BbsI GAAGAC 6 cut(s) 87, 135, 357, 396, 441, 724
Bbv12I GWGCWC 2 cut(s) 228, 570
BbvI GCAGC 2 cut(s) 688, 770
BccI CCATC 2 cut(s) 622, 644
BceAI ACGGC 3 cut(s) 138, 495, 787
BciT130I CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 449, 891, 939
BcnI CCSGG 7 cut(s) 170, 216, 236, 242, 470, 476, 869
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 3 cut(s) 87, 698, 902
BmeT110I CYCGRG 1 cut(s) 569
BmrI ACTGGG 1 cut(s) 1000
BmsI GCATC 2 cut(s) 887, 989
BmtI GCTAGC 1 cut(s) 701
BmuI ACTGGG 1 cut(s) 1000
BpiI GAAGAC 6 cut(s) 87, 135, 357, 396, 441, 724
BpmI CTGGAG 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 348
BpuMI CCSGG 7 cut(s) 170, 216, 236, 242, 470, 476, 869
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 6 cut(s) 22, 31, 142, 448, 889, 937
BsaWI WCCGGW 1 cut(s) 925
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 503, 995
Bse3DI GCAATG 1 cut(s) 711
BseAI TCCGGA 1 cut(s) 925
BseBI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 449, 891, 939
BseDI CCNNGG 6 cut(s) 22, 31, 142, 448, 889, 937
BseGI GGATG 1 cut(s) 636
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 2 cut(s) 503, 995
BsePI GCGCGC 1 cut(s) 1009
BseXI GCAGC 2 cut(s) 688, 770
Bsh1236I CGCG 3 cut(s) 624, 1009, 1011
Bsh1285I CGRYCG 1 cut(s) 1060
BshNI GGYRCC 2 cut(s) 330, 995
BsiEI CGRYCG 1 cut(s) 1060
BsiHKAI GWGCWC 2 cut(s) 228, 570
BsiHKCI CYCGRG 1 cut(s) 569
BsiWI CGTACG 1 cut(s) 512
BslFI GGGAC 1 cut(s) 851
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 851
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 228, 570
Bsp13I TCCGGA 1 cut(s) 925
Bsp143I GATC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 509
BspEI TCCGGA 1 cut(s) 925
BspFNI CGCG 3 cut(s) 624, 1009, 1011
BspOI GCTAGC 1 cut(s) 701
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 348
BspT107I GGYRCC 2 cut(s) 330, 995
BspTNI GGTCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 2 cut(s) 503, 995
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 6 cut(s) 22, 31, 142, 448, 889, 937
BssHII GCGCGC 1 cut(s) 1009
BssMI GATC 1 cut(s) 39
BssNAI GTATAC 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 31
Bst1107I GTATAC 1 cut(s) 359
Bst2UI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 449, 891, 939
Bst4CI ACNGT 2 cut(s) 356, 1030
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 7 cut(s) 152, 186, 224, 458, 699, 1007, 1011
BstDEI CTNAG 2 cut(s) 496, 591
BstEII GGTNACC 1 cut(s) 827
BstF5I GGATG 1 cut(s) 636
BstFNI CGCG 3 cut(s) 624, 1009, 1011
BstHHI GCGC 4 cut(s) 663, 878, 1011, 1013
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 39
BstMCI CGRYCG 1 cut(s) 1060
BstMWI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 490, 950
BstNI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 449, 891, 939
BstNSI RCATGY 1 cut(s) 553
BstPI GGTNACC 1 cut(s) 827
BstUI CGCG 3 cut(s) 624, 1009, 1011
BstV1I GCAGC 2 cut(s) 688, 770
BstV2I GAAGAC 6 cut(s) 87, 135, 357, 396, 441, 724
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 938
BstYI RGATCY 1 cut(s) 39
BstZ17I GTATAC 1 cut(s) 359
BtsCI GGATG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 988
Cac8I GCNNGC 7 cut(s) 152, 186, 224, 458, 699, 1007, 1011
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 4 cut(s) 663, 878, 1011, 1013
Cfr10I RCCGGY 1 cut(s) 184
Csp6I GTAC 5 cut(s) 513, 674, 792, 844, 996
CviAII CATG 5 cut(s) 247, 295, 362, 550, 795
CviQI GTAC 5 cut(s) 513, 674, 792, 844, 996
DdeI CTNAG 2 cut(s) 496, 591
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
DrdI GACNNNNNNGTC 2 cut(s) 137, 443
DseDI GACNNNNNNGTC 2 cut(s) 137, 443
EaeI YGGCCR 4 cut(s) 148, 243, 291, 454
EciI GGCGGA 3 cut(s) 38, 260, 308
Ecl136II GAGCTC 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 7 cut(s) 74, 147, 296, 344, 453, 539, 1036
Eco88I CYCGRG 1 cut(s) 569
Eco91I GGTNACC 1 cut(s) 827
EcoICRI GAGCTC 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 827
EcoRII CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 447, 889, 937
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 5 cut(s) 250, 298, 365, 553, 798
FaqI GGGAC 1 cut(s) 851
FatI CATG 5 cut(s) 246, 294, 361, 549, 794
FauI CCCGC 1 cut(s) 1014
FblI GTMKAC 1 cut(s) 358
FokI GGATG 1 cut(s) 623
FriOI GRGCYC 1 cut(s) 570
FspBI CTAG 3 cut(s) 87, 698, 902
GlaI GCGC 4 cut(s) 662, 877, 1010, 1012
GsuI CTGGAG 1 cut(s) 219
HhaI GCGC 4 cut(s) 663, 878, 1011, 1013
Hin1II CATG 5 cut(s) 250, 298, 365, 553, 798
Hin6I GCGC 4 cut(s) 661, 876, 1009, 1011
HinP1I GCGC 4 cut(s) 661, 876, 1009, 1011
HinfI GANTC 3 cut(s) 392, 526, 710
HphI GGTGA 1 cut(s) 839
Hpy166II GTNNAC 2 cut(s) 359, 920
Hpy188I TCNGA 6 cut(s) 79, 397, 433, 499, 912, 985
Hpy188III TCNNGA 6 cut(s) 198, 530, 689, 714, 857, 926
Hpy8I GTNNAC 2 cut(s) 359, 920
HpyAV CCTTC 4 cut(s) 514, 720, 866, 1060
HpyCH4III ACNGT 2 cut(s) 356, 1030
HpyCH4IV ACGT 3 cut(s) 669, 781, 949
HpyCH4V TGCA 2 cut(s) 704, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 490, 950
HpyF3I CTNAG 2 cut(s) 496, 591
HpySE526I ACGT 3 cut(s) 669, 781, 949
Hsp92II CATG 5 cut(s) 250, 298, 365, 553, 798
HspAI GCGC 4 cut(s) 661, 876, 1009, 1011
Kpn2I TCCGGA 1 cut(s) 925
KpnI GGTACC 1 cut(s) 999
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 2 cut(s) 688, 770
LweI GCATC 2 cut(s) 887, 989
MaeI CTAG 3 cut(s) 87, 698, 902
MaeII ACGT 3 cut(s) 669, 781, 949
MaeIII GTNAC 4 cut(s) 118, 382, 827, 857
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 6 cut(s) 92, 140, 362, 401, 446, 729
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 2 cut(s) 228, 570
MluCI AATT 2 cut(s) 286, 913
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 57, 890
MnlI CCTC 7 cut(s) 205, 259, 439, 505, 595, 643, 657
MroI TCCGGA 1 cut(s) 925
MroNI GCCGGC 1 cut(s) 184
MseI TTAA 2 cut(s) 666, 819
MslI CAYNNNNRTG 1 cut(s) 939
MvaI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 449, 891, 939
MvnI CGCG 3 cut(s) 624, 1009, 1011
MwoI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 490, 950
NaeI GCCGGC 1 cut(s) 186
NciI CCSGG 7 cut(s) 170, 216, 236, 242, 470, 476, 869
NdeII GATC 1 cut(s) 39
NgoMIV GCCGGC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 697
NlaIII CATG 5 cut(s) 250, 298, 365, 553, 798
NmuCI GTSAC 4 cut(s) 118, 382, 827, 857
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 348
OliI CACNNNNGTG 1 cut(s) 939
PaeR7I CTCGAG 1 cut(s) 569
PauI GCGCGC 1 cut(s) 1009
PciI ACATGT 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PdiI GCCGGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 526, 710
Pfl23II CGTACG 1 cut(s) 512
PflFI GACNNNGTC 3 cut(s) 170, 476, 863
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 867
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
PscI ACATGT 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 570
Psp6I CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 447, 889, 937
PspEI GGTNACC 1 cut(s) 827
PspGI CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 447, 889, 937
PspLI CGTACG 1 cut(s) 512
PspXI VCTCGAGB 1 cut(s) 569
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 39
PsyI GACNNNGTC 3 cut(s) 170, 476, 863
PteI GCGCGC 1 cut(s) 1009
RsaI GTAC 5 cut(s) 514, 675, 793, 845, 997
RsaNI GTAC 5 cut(s) 513, 674, 792, 844, 996
RseI CAYNNNNRTG 1 cut(s) 939
SacI GAGCTC 1 cut(s) 570
SaqAI TTAA 2 cut(s) 666, 819
Sau3AI GATC 1 cut(s) 39
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 2 cut(s) 228, 570
SfaNI GCATC 2 cut(s) 887, 989
Sfr274I CTCGAG 1 cut(s) 569
SfuI TTCGAA 1 cut(s) 348
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SpeI ACTAGT 1 cut(s) 901
Sse9I AATT 2 cut(s) 286, 913
SspMI CTAG 3 cut(s) 87, 698, 902
SstI GAGCTC 1 cut(s) 570
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 356, 1030
TaiI ACGT 3 cut(s) 672, 784, 952
TaqI TCGA 6 cut(s) 264, 348, 531, 570, 715, 777
TasI AATT 2 cut(s) 286, 913
TatI WGTACW 3 cut(s) 673, 791, 843
TauI GCSGC 8 cut(s) 61, 150, 222, 253, 331, 412, 456, 543
TfiI GAWTC 2 cut(s) 526, 710
Tru1I TTAA 2 cut(s) 666, 819
Tru9I TTAA 2 cut(s) 666, 819
TscAI CASTG 1 cut(s) 995
TseFI GTSAC 4 cut(s) 118, 382, 827, 857
TseI GCWGC 2 cut(s) 701, 758
Tsp45I GTSAC 4 cut(s) 118, 382, 827, 857
TspDTI ATGAA 1 cut(s) 961
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 995
Tth111I GACNNNGTC 3 cut(s) 170, 476, 863
Van91I CCANNNNNTGG 1 cut(s) 43
XceI RCATGY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XhoI CTCGAG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 358
XspI CTAG 3 cut(s) 87, 698, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.