Rh5DG100700

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012572
Physical Location & Seq
Forward (+)
9207243 .. 9210765
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG100700.1

Sequence Viewer

Length: 933 bp
ATGCCCCTATCCCCTGTCAAGGAAGACCCTGATGGCCCGAAGCGGCCCCGTTCGTCCTTGTTTGAAGACCCTAGCCAGGACCAGAACCTAACCCTAACCCCTGTCACGGAAGACCCTGTTGTCCACGACCCGGACCCAGCTATGCCCCTATCTCCTATCGTGGTCCGCCTGAAGCGGCACCGTTCGTCCTTGTTCGAAGACCCTAGCCACGACCAGAACCTAACCCCTGTCACGGAAGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTGTCCTCTGAACACCCTGATATCCAGGGCGGCAAGCACCAGGACCCGGACCCGGCGAAGCCCTTAAAACCAGTAAGCCGTACGCTGAAGGAGATTCTGGATGAGGCCGGCCCGCTCATCTCTAAAGAGCGAGAGCTCGAGTATGCTAAGATTTTTGCTAAGCGGAAGGAGGCCGAACCTATTACCAATCGCGTGACGGGGTTTGGTGGACCCATTAACGTAGTACAGAGTTATGAAACCGAAGCTGCTCGTGCTAGCAAGAGGACACTCCCTGATACACCTTTTTGGAATACTCTTGCTTCTCTGAGACGTAGAAGTGCAGAAAACCCAAAGTATTGTGGAAAAGAAGCATGGAAAGAATTCATCGAACGTCAAAGGAGTACATGGGGGCCGTGTAAGATTTGTTTGAAGGCTGATGACCATATGAGTTTGCACTGTCCATATCTCGACTATGTCCCGGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTAGGGGTTGTGGACTTAAGGACAAGCACCCTGGTATGAATTGGTCTAAATGTGCTATTCTCAAGTCGTGTTTCATTTGTGCATCAGACCACTGGGGTACTGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCACTCCCTCTAGCGACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.63

Weight (kDa)

7.58

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 385
AccII CGCG 2 cut(s) 462, 877
AciI CCGC 9 cut(s) 43, 166, 175, 256, 300, 383, 433, 875, 928
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 2 cut(s) 191, 377
AfaI GTAC 4 cut(s) 352, 495, 652, 865
AfiI CCNNNNNNNGG 7 cut(s) 19, 76, 106, 130, 232, 316, 322
AflII CTTAAG 1 cut(s) 782
AgsI TTSAA 3 cut(s) 65, 679, 758
AhlI ACTAGT 1 cut(s) 760
AjnI CCWGG 5 cut(s) 75, 294, 309, 748, 796
AluBI AGCT 3 cut(s) 140, 406, 515
AluI AGCT 3 cut(s) 140, 406, 515
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 571
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 8 cut(s) 34, 44, 257, 375, 379, 441, 659, 745
ApeKI GCWGC 1 cut(s) 515
ApoI RAATTY 1 cut(s) 629
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 1 cut(s) 737
AsuC2I CCSGG 4 cut(s) 131, 317, 323, 728
AsuII TTCGAA 1 cut(s) 195
AsuNHI GCTAGC 1 cut(s) 524
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 6 cut(s) 79, 133, 163, 313, 319, 479
BanI GGYRCC 1 cut(s) 177
BanII GRGCYC 1 cut(s) 408
BauI CACGAG 1 cut(s) 519
BbsI GAAGAC 5 cut(s) 30, 72, 117, 204, 243
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 502
BccI CCATC 1 cut(s) 26
BceAI ACGGC 2 cut(s) 333, 646
BciT130I CCWGG 5 cut(s) 77, 296, 311, 750, 798
BcnI CCSGG 4 cut(s) 131, 317, 323, 728
BcoDI GTCTC 1 cut(s) 571
BcuI ACTAGT 1 cut(s) 760
BfaI CTAG 5 cut(s) 72, 204, 525, 761, 921
BfrI CTTAAG 1 cut(s) 782
BisI GCNGC 5 cut(s) 44, 176, 257, 301, 516
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 5 cut(s) 45, 177, 258, 302, 517
Bme1390I CCNGG 9 cut(s) 77, 131, 296, 311, 317, 323, 728, 750, 798
Bme18I GGWCC 6 cut(s) 79, 133, 163, 313, 319, 479
BmeT110I CYCGRG 1 cut(s) 407
BmiI GGNNCC 9 cut(s) 47, 135, 179, 260, 315, 321, 481, 660, 900
BmrFI CCNGG 9 cut(s) 77, 131, 296, 311, 317, 323, 728, 750, 798
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 2 cut(s) 746, 857
BmtI GCTAGC 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 868
BpiI GAAGAC 5 cut(s) 30, 72, 117, 204, 243
Bpu1102I GCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 4 cut(s) 131, 317, 323, 728
BsaJI CCNNGG 3 cut(s) 295, 748, 796
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 76, 106, 130, 232, 316, 322
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 341, 863
BseBI CCWGG 5 cut(s) 77, 296, 311, 750, 798
BseDI CCNNGG 3 cut(s) 295, 748, 796
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 7 cut(s) 19, 76, 106, 130, 232, 316, 322
BseMII CTCAG 1 cut(s) 566
BseNI ACTGG 2 cut(s) 341, 863
BseXI GCAGC 1 cut(s) 502
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 2 cut(s) 462, 877
Bsh1285I CGRYCG 1 cut(s) 928
BshFI GGCC 8 cut(s) 36, 46, 259, 377, 381, 443, 661, 747
BshNI GGYRCC 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 928
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 5 cut(s) 131, 317, 323, 378, 728
BsiWI CGTACG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 7 cut(s) 19, 76, 106, 130, 232, 316, 322
BsmAI GTCTC 1 cut(s) 571
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 710
BsnI GGCC 8 cut(s) 36, 46, 259, 377, 381, 443, 661, 747
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 408
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 9 cut(s) 43, 166, 175, 256, 300, 383, 433, 875, 928
BspANI GGCC 8 cut(s) 36, 46, 259, 377, 381, 443, 661, 747
BspCNI CTCAG 1 cut(s) 567
BspFNI CGCG 2 cut(s) 462, 877
BspLI GGNNCC 9 cut(s) 47, 135, 179, 260, 315, 321, 481, 660, 900
BspOI GCTAGC 1 cut(s) 528
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 782
BsrBI CCGCTC 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 2 cut(s) 341, 863
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 3 cut(s) 295, 748, 796
BssSI CACGAG 1 cut(s) 519
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 5 cut(s) 77, 296, 311, 750, 798
Bst4CI ACNGT 3 cut(s) 182, 707, 898
BstAFI CTTAAG 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 6 cut(s) 305, 379, 383, 526, 875, 879
BstDEI CTNAG 3 cut(s) 417, 429, 575
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 2 cut(s) 462, 877
BstHHI GCGC 1 cut(s) 737
BstMAI GTCTC 1 cut(s) 571
BstMCI CGRYCG 1 cut(s) 928
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNI CCWGG 5 cut(s) 77, 296, 311, 750, 798
BstSCI CCNGG 9 cut(s) 75, 129, 294, 309, 315, 321, 726, 748, 796
BstUI CGCG 2 cut(s) 462, 877
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 5 cut(s) 30, 72, 117, 204, 243
BsuRI GGCC 8 cut(s) 36, 46, 259, 377, 381, 443, 661, 747
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 703, 856
Cac8I GCNNGC 6 cut(s) 305, 379, 383, 526, 875, 879
CfoI GCGC 1 cut(s) 737
Cfr10I RCCGGY 1 cut(s) 377
Csp6I GTAC 4 cut(s) 351, 494, 651, 864
CviAII CATG 2 cut(s) 621, 654
CviQI GTAC 4 cut(s) 351, 494, 651, 864
DdeI CTNAG 3 cut(s) 417, 429, 575
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 408
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 6 cut(s) 79, 133, 163, 313, 319, 479
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 191, 377
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 5 cut(s) 75, 294, 309, 748, 796
EcoRV GATATC 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 571
FaeI CATG 2 cut(s) 624, 657
FaqI GGGAC 1 cut(s) 710
FatI CATG 2 cut(s) 620, 653
FauI CCCGC 2 cut(s) 390, 882
FauNDI CATATG 1 cut(s) 693
Fnu4HI GCNGC 5 cut(s) 44, 176, 257, 301, 516
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 408
FseI GGCCGGCC 1 cut(s) 381
Fsp4HI GCNGC 5 cut(s) 44, 176, 257, 301, 516
FspBI CTAG 5 cut(s) 72, 204, 525, 761, 921
GlaI GCGC 1 cut(s) 736
GluI GCNGC 5 cut(s) 44, 176, 257, 301, 516
GsaI CCCAGC 1 cut(s) 140
HaeIII GGCC 8 cut(s) 36, 46, 259, 377, 381, 443, 661, 747
HapII CCGG 5 cut(s) 131, 317, 323, 378, 728
HhaI GCGC 1 cut(s) 737
Hin1II CATG 2 cut(s) 624, 657
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HinfI GANTC 2 cut(s) 239, 364
HpaII CCGG 5 cut(s) 131, 317, 323, 378, 728
Hpy166II GTNNAC 3 cut(s) 124, 479, 779
Hpy188I TCNGA 4 cut(s) 244, 280, 576, 853
Hpy188III TCNNGA 2 cut(s) 368, 716
Hpy8I GTNNAC 3 cut(s) 124, 479, 779
HpyAV CCTTC 4 cut(s) 352, 430, 673, 725
HpyCH4III ACNGT 3 cut(s) 182, 707, 898
HpyCH4IV ACGT 3 cut(s) 489, 580, 640
HpyCH4V TGCA 3 cut(s) 590, 703, 848
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 3 cut(s) 417, 429, 575
HpySE526I ACGT 3 cut(s) 489, 580, 640
Hsp92II CATG 2 cut(s) 624, 657
HspAI GCGC 1 cut(s) 735
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 2 cut(s) 746, 857
MaeI CTAG 5 cut(s) 72, 204, 525, 761, 921
MaeII ACGT 3 cut(s) 489, 580, 640
MaeIII GTNAC 3 cut(s) 103, 229, 463
MbiI CCGCTC 1 cut(s) 385
MboII GAAGA 5 cut(s) 35, 77, 122, 209, 248
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 629, 805
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 5 cut(s) 286, 367, 433, 525, 928
MroNI GCCGGC 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 629
MseI TTAA 3 cut(s) 335, 486, 783
MspA1I CMGCKG 1 cut(s) 930
MspCI CTTAAG 1 cut(s) 782
MspI CCGG 5 cut(s) 131, 317, 323, 378, 728
MspR9I CCNGG 9 cut(s) 77, 131, 296, 311, 317, 323, 728, 750, 798
MvaI CCWGG 5 cut(s) 77, 296, 311, 750, 798
MvnI CGCG 2 cut(s) 462, 877
MwoI GCNNNNNNNGC 1 cut(s) 521
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 4 cut(s) 131, 317, 323, 728
NdeI CATATG 1 cut(s) 693
NgoMIV GCCGGC 1 cut(s) 377
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 2 cut(s) 624, 657
NlaIV GGNNCC 9 cut(s) 47, 135, 179, 260, 315, 321, 481, 660, 900
NmuCI GTSAC 3 cut(s) 103, 229, 463
NspV TTCGAA 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 407
PdiI GCCGGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 364
Pfl23II CGTACG 1 cut(s) 350
PflFI GACNNNGTC 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 726
PkrI GCNGC 5 cut(s) 45, 177, 258, 302, 517
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PpuMI RGGWCCY 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 5 cut(s) 75, 294, 309, 748, 796
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 5 cut(s) 75, 294, 309, 748, 796
PspLI CGTACG 1 cut(s) 350
PspN4I GGNNCC 9 cut(s) 47, 135, 179, 260, 315, 321, 481, 660, 900
PspPPI RGGWCCY 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 407
PsyI GACNNNGTC 1 cut(s) 722
RigI GGCCGGCC 1 cut(s) 381
RsaI GTAC 4 cut(s) 352, 495, 652, 865
RsaNI GTAC 4 cut(s) 351, 494, 651, 864
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 335, 486, 783
SatI GCNGC 5 cut(s) 44, 176, 257, 301, 516
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 9 cut(s) 77, 131, 296, 311, 317, 323, 728, 750, 798
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 2 cut(s) 746, 857
Sfr274I CTCGAG 1 cut(s) 407
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 6 cut(s) 79, 133, 163, 313, 319, 479
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 3 cut(s) 407, 782, 827
SmoI CTYRAG 3 cut(s) 407, 782, 827
SpeI ACTAGT 1 cut(s) 760
Sse9I AATT 2 cut(s) 629, 805
SsiI CCGC 9 cut(s) 43, 166, 175, 256, 300, 383, 433, 875, 928
SspMI CTAG 5 cut(s) 72, 204, 525, 761, 921
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 9 cut(s) 75, 129, 294, 309, 315, 321, 726, 748, 796
TaaI ACNGT 3 cut(s) 182, 707, 898
TaiI ACGT 3 cut(s) 492, 583, 643
TaqI TCGA 4 cut(s) 195, 408, 636, 717
TasI AATT 2 cut(s) 629, 805
TatI WGTACW 2 cut(s) 493, 650
TauI GCSGC 4 cut(s) 46, 178, 259, 303
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 335, 486, 783
Tru9I TTAA 3 cut(s) 335, 486, 783
TscAI CASTG 2 cut(s) 710, 863
TseFI GTSAC 3 cut(s) 103, 229, 463
TseI GCWGC 1 cut(s) 515
Tsp45I GTSAC 3 cut(s) 103, 229, 463
TspDTI ATGAA 4 cut(s) 519, 622, 818, 829
TspGWI ACGGA 2 cut(s) 122, 248
TspRI CASTG 2 cut(s) 710, 863
Tth111I GACNNNGTC 1 cut(s) 722
Vha464I CTTAAG 1 cut(s) 782
VpaK11BI GGWCC 6 cut(s) 79, 133, 163, 313, 319, 479
XapI RAATTY 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 407
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 5 cut(s) 72, 204, 525, 761, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.