Rh5CG114200

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012572
Physical Location & Seq
Forward (+)
9359231 .. 9361903
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG114200.1

Sequence Viewer

Length: 1161 bp
ATGCCCCTATCCCCTGATGTCCAGGGCGACCAAGGCCAGATCCTGGTCCGCCTGAAGCGGCCCCATTCGTCCTCGTCGGAAGACCCTAGCCAGGACCAGAACCTAACCCTAACCCCTGTCACGGCTGAAGACCTTGATGTCCAGGGCGGCCAGCACCAGGAGCCGGACCCGGTCCAGGTCCAGCCGGCGAAGCCCTCTGGAGACCCTGATGTTCCGGGCGGCGAGCACCATGACCCGGACCCGGCCATGCCCCTATCCCCTATCGAGGTCCGCCTGAAGCGGCACCATTCGTCCTTGTTCGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTGTTCTCTGAAGACCCTGATGTTCCGGGCGGCCAGCACCACGACCCGGACCCGGCCATGCCCCTATCCCCTATAGTGGTCCGCCTGAAGCGGCACAATTCGTCCTTGTTCGAAGACCGTATACATGACCAGAACCTAACCCCTGTCACGGAAGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTATCCTCTGAAGACCCTGATGTCCAGGGCGGCCAGCACCAGAACCCGGACCCGGTCCAGCCCGTGAAGCCCTCAGAACCAGTAAGCCGTACGCTGAAGGAGATTCTCGATGAGTGCGGCCCGAACATGTCTAAAGAGCAAGAGCTCGAGTATGCTATGCTTTTTGCTAAGTGGAAAGAGGCCGAACCTATTACCAATCGCGTGATGGCCTCCATCCCGGAGCCTCTTTTTGGTGGACGCATTAACGTAGTACAGAGTTATGGTCCTGATACGCTAGCTGCAATGGATTCTCGAAGACAAAGAAGGGCAGAAAACCCAAAGTATTATGGAGAAGCAGCATTGAACGAATACATCGAACGTCAAAAGAGTACATGGGGGCCGTGTGAGATTTGTTTTAAGGCTGGTGACCATATTAGTTTGTACTGCCCATATCGTGACTATGTCCCCGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTCGGAATTGTGGACTTCCGGACCAGCACCCTGGTGCTAAACGTGCTATTCGCAAGTCGTGTTTCATTTGTGCATCAGACCACTGGGGTACTGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCTTTCCCTTCAGCGACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

386

Amino Acids

42.86

Weight (kDa)

5.46

Isoelectric Point (pI)

64.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 539
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 1 cut(s) 454
AccII CGCG 2 cut(s) 720, 1105
AccIII TCCGGA 1 cut(s) 1021
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 5 cut(s) 148, 243, 364, 387, 550
AcuI CTGAAG 8 cut(s) 74, 147, 296, 363, 440, 549, 635, 1132
AfaI GTAC 5 cut(s) 610, 771, 889, 941, 1093
AflIII ACRYGT 1 cut(s) 645
AgsI TTSAA 2 cut(s) 862, 995
AhlI ACTAGT 1 cut(s) 997
AjnI CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 543, 985, 1033
AleI CACNNNNGTG 1 cut(s) 1035
AluBI AGCT 2 cut(s) 664, 797
AluI AGCT 2 cut(s) 664, 797
Alw21I GWGCWC 2 cut(s) 228, 666
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 34
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 665
Aor13HI TCCGGA 1 cut(s) 1021
ApeKI GCWGC 2 cut(s) 797, 854
AspLEI GCGC 1 cut(s) 974
AsuHPI GGTGA 1 cut(s) 935
AsuII TTCGAA 1 cut(s) 444
AsuNHI GCTAGC 1 cut(s) 793
AvaI CYCGRG 1 cut(s) 665
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 666
BarI GAAGNNNNNNTAC 2 cut(s) 438, 470
BbsI GAAGAC 7 cut(s) 87, 135, 351, 453, 492, 537, 820
Bbv12I GWGCWC 2 cut(s) 228, 666
BbvI GCAGC 2 cut(s) 784, 866
BccI CCATC 2 cut(s) 718, 740
BceAI ACGGC 3 cut(s) 138, 591, 883
BciT130I CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 545, 987, 1035
BcoDI GTCTC 1 cut(s) 195
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 3 cut(s) 87, 794, 998
BfmI CTRYAG 1 cut(s) 405
BmeT110I CYCGRG 1 cut(s) 665
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 2 cut(s) 983, 1085
BmtI GCTAGC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 1096
BpiI GAAGAC 7 cut(s) 87, 135, 351, 453, 492, 537, 820
BpmI CTGGAG 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 6 cut(s) 22, 31, 142, 544, 985, 1033
BsaWI WCCGGW 1 cut(s) 1021
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 599, 1091
Bse3DI GCAATG 1 cut(s) 807
BseAI TCCGGA 1 cut(s) 1021
BseBI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 545, 987, 1035
BseDI CCNNGG 6 cut(s) 22, 31, 142, 544, 985, 1033
BseGI GGATG 1 cut(s) 732
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 599, 1091
BseXI GCAGC 2 cut(s) 784, 866
Bsh1236I CGCG 2 cut(s) 720, 1105
Bsh1285I CGRYCG 1 cut(s) 1156
BshNI GGYRCC 1 cut(s) 282
BsiEI CGRYCG 1 cut(s) 1156
BsiHKAI GWGCWC 2 cut(s) 228, 666
BsiHKCI CYCGRG 1 cut(s) 665
BsiWI CGTACG 1 cut(s) 608
BslFI GGGAC 1 cut(s) 947
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 947
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 228, 666
Bsp13I TCCGGA 1 cut(s) 1021
Bsp143I GATC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 605
BspEI TCCGGA 1 cut(s) 1021
BspFNI CGCG 2 cut(s) 720, 1105
BspOI GCTAGC 1 cut(s) 797
BspPI GGATC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 444
BspT107I GGYRCC 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 2 cut(s) 599, 1091
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 6 cut(s) 22, 31, 142, 544, 985, 1033
BssMI GATC 1 cut(s) 39
BssNAI GTATAC 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 31
Bst1107I GTATAC 1 cut(s) 455
Bst2UI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 545, 987, 1035
Bst4CI ACNGT 2 cut(s) 452, 1126
BstBI TTCGAA 1 cut(s) 444
BstC8I GCNNGC 8 cut(s) 152, 186, 224, 368, 554, 795, 1103, 1107
BstDEI CTNAG 2 cut(s) 592, 687
BstEII GGTNACC 1 cut(s) 923
BstF5I GGATG 1 cut(s) 732
BstFNI CGCG 2 cut(s) 720, 1105
BstHHI GCGC 1 cut(s) 974
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 39
BstMCI CGRYCG 1 cut(s) 1156
BstMWI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 586, 1046
BstNI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 545, 987, 1035
BstNSI RCATGY 1 cut(s) 649
BstPI GGTNACC 1 cut(s) 923
BstSFI CTRYAG 1 cut(s) 405
BstUI CGCG 2 cut(s) 720, 1105
BstV1I GCAGC 2 cut(s) 784, 866
BstV2I GAAGAC 7 cut(s) 87, 135, 351, 453, 492, 537, 820
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 39
BstZ17I GTATAC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 732
BtsIMutI CAGTG 1 cut(s) 1084
Cac8I GCNNGC 8 cut(s) 152, 186, 224, 368, 554, 795, 1103, 1107
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 1 cut(s) 974
Cfr10I RCCGGY 1 cut(s) 184
CseI GACGC 1 cut(s) 765
Csp6I GTAC 5 cut(s) 609, 770, 888, 940, 1092
CviAII CATG 6 cut(s) 230, 247, 391, 458, 646, 891
CviQI GTAC 5 cut(s) 609, 770, 888, 940, 1092
DdeI CTNAG 2 cut(s) 592, 687
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
DrdI GACNNNNNNGTC 2 cut(s) 137, 539
DseDI GACNNNNNNGTC 2 cut(s) 137, 539
EaeI YGGCCR 5 cut(s) 148, 243, 364, 387, 550
EciI GGCGGA 3 cut(s) 38, 260, 404
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 8 cut(s) 74, 147, 296, 363, 440, 549, 635, 1132
Eco88I CYCGRG 1 cut(s) 665
Eco91I GGTNACC 1 cut(s) 923
EcoICRI GAGCTC 1 cut(s) 664
EcoO65I GGTNACC 1 cut(s) 923
EcoRII CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 543, 985, 1033
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 6 cut(s) 233, 250, 394, 461, 649, 894
FaqI GGGAC 1 cut(s) 947
FatI CATG 6 cut(s) 229, 246, 390, 457, 645, 890
FauI CCCGC 1 cut(s) 1110
FblI GTMKAC 1 cut(s) 454
FokI GGATG 1 cut(s) 719
FriOI GRGCYC 1 cut(s) 666
FspBI CTAG 3 cut(s) 87, 794, 998
GlaI GCGC 1 cut(s) 973
GsuI CTGGAG 1 cut(s) 219
HgaI GACGC 1 cut(s) 765
HhaI GCGC 1 cut(s) 974
Hin1II CATG 6 cut(s) 233, 250, 394, 461, 649, 894
Hin6I GCGC 1 cut(s) 972
HinP1I GCGC 1 cut(s) 972
HinfI GANTC 4 cut(s) 302, 488, 622, 806
HphI GGTGA 1 cut(s) 935
Hpy166II GTNNAC 3 cut(s) 455, 755, 1016
Hpy188I TCNGA 8 cut(s) 79, 307, 343, 493, 529, 595, 1008, 1081
Hpy188III TCNNGA 6 cut(s) 198, 626, 785, 810, 953, 1022
Hpy8I GTNNAC 3 cut(s) 455, 755, 1016
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 4 cut(s) 610, 816, 962, 1156
HpyCH4III ACNGT 2 cut(s) 452, 1126
HpyCH4IV ACGT 3 cut(s) 765, 877, 1045
HpyCH4V TGCA 2 cut(s) 800, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 586, 1046
HpyF3I CTNAG 2 cut(s) 592, 687
HpySE526I ACGT 3 cut(s) 765, 877, 1045
Hsp92II CATG 6 cut(s) 233, 250, 394, 461, 649, 894
HspAI GCGC 1 cut(s) 972
Kpn2I TCCGGA 1 cut(s) 1021
KroI GCCGGC 1 cut(s) 184
KroNI GCCGGC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 160, 739
Lsp1109I GCAGC 2 cut(s) 784, 866
LweI GCATC 2 cut(s) 983, 1085
MaeI CTAG 3 cut(s) 87, 794, 998
MaeII ACGT 3 cut(s) 765, 877, 1045
MaeIII GTNAC 4 cut(s) 118, 478, 923, 953
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 7 cut(s) 92, 140, 356, 458, 497, 542, 825
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 2 cut(s) 228, 666
MluCI AATT 2 cut(s) 430, 1009
MlyI GAGTC 2 cut(s) 296, 482
MmeI TCCRAC 2 cut(s) 57, 986
MnlI CCTC 8 cut(s) 82, 205, 259, 535, 601, 691, 739, 753
MroI TCCGGA 1 cut(s) 1021
MroNI GCCGGC 1 cut(s) 184
MseI TTAA 2 cut(s) 762, 915
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 1 cut(s) 1158
MvaI CCWGG 9 cut(s) 23, 44, 92, 143, 158, 176, 545, 987, 1035
MvnI CGCG 2 cut(s) 720, 1105
MwoI GCNNNNNNNGC 5 cut(s) 33, 160, 190, 586, 1046
NaeI GCCGGC 1 cut(s) 186
NdeII GATC 1 cut(s) 39
NgoMIV GCCGGC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 793
NlaIII CATG 6 cut(s) 233, 250, 394, 461, 649, 894
NmuCI GTSAC 4 cut(s) 118, 478, 923, 953
NspI RCATGY 1 cut(s) 649
NspV TTCGAA 1 cut(s) 444
OliI CACNNNNGTG 1 cut(s) 1035
PaeR7I CTCGAG 1 cut(s) 665
PciI ACATGT 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PdiI GCCGGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 622, 806
Pfl23II CGTACG 1 cut(s) 608
PflFI GACNNNGTC 3 cut(s) 170, 572, 959
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 735
PleI GAGTC 2 cut(s) 296, 482
PpsI GAGTC 2 cut(s) 296, 482
PscI ACATGT 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 543, 985, 1033
PspEI GGTNACC 1 cut(s) 923
PspGI CCWGG 9 cut(s) 21, 42, 90, 141, 156, 174, 543, 985, 1033
PspLI CGTACG 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 665
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 39
PsyI GACNNNGTC 3 cut(s) 170, 572, 959
RsaI GTAC 5 cut(s) 610, 771, 889, 941, 1093
RsaNI GTAC 5 cut(s) 609, 770, 888, 940, 1092
RseI CAYNNNNRTG 1 cut(s) 1035
SacI GAGCTC 1 cut(s) 666
SaqAI TTAA 2 cut(s) 762, 915
Sau3AI GATC 1 cut(s) 39
SchI GAGTC 2 cut(s) 296, 482
SduI GDGCHC 2 cut(s) 228, 666
SfaNI GCATC 2 cut(s) 983, 1085
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 665
SfuI TTCGAA 1 cut(s) 444
SlaI CTCGAG 1 cut(s) 665
SmiMI CAYNNNNRTG 1 cut(s) 1035
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SpeI ACTAGT 1 cut(s) 997
Sse9I AATT 2 cut(s) 430, 1009
SspMI CTAG 3 cut(s) 87, 794, 998
SstI GAGCTC 1 cut(s) 666
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 452, 1126
TaiI ACGT 3 cut(s) 768, 880, 1048
TaqI TCGA 7 cut(s) 264, 300, 444, 627, 666, 811, 873
TasI AATT 2 cut(s) 430, 1009
TatI WGTACW 3 cut(s) 769, 887, 939
TfiI GAWTC 2 cut(s) 622, 806
Tru1I TTAA 2 cut(s) 762, 915
Tru9I TTAA 2 cut(s) 762, 915
TscAI CASTG 1 cut(s) 1091
TseFI GTSAC 4 cut(s) 118, 478, 923, 953
TseI GCWGC 2 cut(s) 797, 854
Tsp45I GTSAC 4 cut(s) 118, 478, 923, 953
TspDTI ATGAA 1 cut(s) 1057
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 1 cut(s) 1091
Tth111I GACNNNGTC 3 cut(s) 170, 572, 959
Van91I CCANNNNNTGG 1 cut(s) 43
XceI RCATGY 1 cut(s) 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XhoI CTCGAG 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 454
XspI CTAG 3 cut(s) 87, 794, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.