Rh5AG105900

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012572
Physical Location & Seq
Forward (+)
9697640 .. 9701259
3620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG105900.1

Sequence Viewer

Length: 1137 bp
ATGCCCCTATCCCCTGTCAAGGAAGACCCTGATGGCCCGAAGCGGCCCCGTTCGTCCTTGTTTGAAGACCCTAGCCAGGACCAGAACCTAACCCTAACCCCTGTCACGGAAGACCCTGTTGTCCACGACCCGGACCCAGCTATGCCCCTATCCCCTATCGTGGTCCGCCTGAAGCGGCACCGTTCGTCCTTGTTCGAAGACCCTAGCCACGACCAGAACCTAACCCCTGTCACGGAAGACTCTGATGTTGCGAAGCGGCCCCGTTCGTCCTTGTCCTCTGAACACCCTGATATCCAGGGCGGCAAGCACCAGGACCCGGACCCGGCGAAGCCCTTAAAACCAGTAAGCCGTACGCTGAAGGAGATTCTGGATGAGGCCGGCCCGCTCATCTCTAAAGAGCGAGAGCGAGAGTATGCTAAGATTTTTGCTAAGCGGAAGGAGGCCGAACCTATTACCAATCGCGTGACGGGGTTTGGTGGACCCATTAACGTAGTACAGAGTTATGAGGCCAAACCTATTACCAATCGCGTGATGGCCTCCTTCCCGGAGCCTCTTTTGGGTGGACGCATTAACGTAGTACAGAGTTATGGTCCTGATACACTAGCTGAAGCCGCAGCTGCTGGTGCTAGCAAGAGGACACTCCGTGATACACCTCTTTTTGGTGGATGCATTAACGTAGAAGAGAGTTATGGTCCTGATACAGTAGCTGAAACCGAAGCTGCTCGTGCTAGCAAGAGGACACTCCCTGATACACCTTTTTGGAATACTCTTGATTCTCTGAGACGTAGAAGTGCAGAAAACCCAAAGTATTGTGGAAAAGAAGCATGGAAAGAATTCATCGAACGTCAAAGGAGTACATGGGGGCCGTGTAAGATTTGTTTGAAGGCTGATGACCATATGAGTTTGCACTGTCCATATCTCGACTATGTCCCGGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTAGGGGTTGTGGACTTAAGGACAAGCACCCTGGTATGAATTGGTCTAAATGTGCTATTCTCAAGTCGTGTTTCATTTGTGCATCAGACCACTGGGGTACTGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCACTCCCTTTAGCGACCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

378

Amino Acids

41.85

Weight (kDa)

6.8

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 385
AccII CGCG 3 cut(s) 462, 528, 1081
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 3 cut(s) 191, 377, 627
AdeI CACNNNGTG 1 cut(s) 644
AfaI GTAC 5 cut(s) 352, 495, 579, 856, 1069
AflII CTTAAG 1 cut(s) 986
AgsI TTSAA 3 cut(s) 65, 883, 962
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 5 cut(s) 75, 294, 309, 952, 1000
AluBI AGCT 5 cut(s) 140, 605, 617, 707, 719
AluI AGCT 5 cut(s) 140, 605, 617, 707, 719
Alw26I GTCTC 1 cut(s) 775
AlwNI CAGNNNCTG 2 cut(s) 620, 707
ApeKI GCWGC 3 cut(s) 614, 617, 719
ApoI RAATTY 1 cut(s) 833
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 941
AsuC2I CCSGG 5 cut(s) 131, 317, 323, 545, 932
AsuII TTCGAA 1 cut(s) 195
AsuNHI GCTAGC 2 cut(s) 626, 728
AvaII GGWCC 8 cut(s) 79, 133, 163, 313, 319, 479, 590, 692
BanI GGYRCC 1 cut(s) 177
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 5 cut(s) 30, 72, 117, 204, 243
BbvI GCAGC 3 cut(s) 604, 626, 706
BccI CCATC 2 cut(s) 26, 526
BceAI ACGGC 2 cut(s) 333, 850
BciT130I CCWGG 5 cut(s) 77, 296, 311, 954, 1002
BcnI CCSGG 5 cut(s) 131, 317, 323, 545, 932
BcoDI GTCTC 1 cut(s) 775
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 6 cut(s) 72, 204, 602, 627, 729, 965
BfrI CTTAAG 1 cut(s) 986
BisI GCNGC 8 cut(s) 44, 176, 257, 301, 612, 615, 618, 720
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 8 cut(s) 45, 177, 258, 302, 613, 616, 619, 721
Bme18I GGWCC 8 cut(s) 79, 133, 163, 313, 319, 479, 590, 692
BmrI ACTGGG 1 cut(s) 1072
BmsI GCATC 3 cut(s) 656, 950, 1061
BmtI GCTAGC 2 cut(s) 630, 732
BmuI ACTGGG 1 cut(s) 1072
BpiI GAAGAC 5 cut(s) 30, 72, 117, 204, 243
Bpu1102I GCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 1016
BpuMI CCSGG 5 cut(s) 131, 317, 323, 545, 932
BsaJI CCNNGG 3 cut(s) 295, 952, 1000
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 341, 1067
BseBI CCWGG 5 cut(s) 77, 296, 311, 954, 1002
BseDI CCNNGG 3 cut(s) 295, 952, 1000
BseGI GGATG 2 cut(s) 376, 671
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 341, 1067
BseXI GCAGC 3 cut(s) 604, 626, 706
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 813
Bsh1236I CGCG 3 cut(s) 462, 528, 1081
Bsh1285I CGRYCG 1 cut(s) 1132
BshNI GGYRCC 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 1132
BsiSI CCGG 6 cut(s) 131, 317, 323, 378, 545, 932
BsiWI CGTACG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 914
BsmAI GTCTC 1 cut(s) 775
BsmBI CGTCTC 1 cut(s) 775
BsmFI GGGAC 1 cut(s) 914
Bsp119I TTCGAA 1 cut(s) 195
Bsp1720I GCTNAGC 1 cut(s) 429
BspCNI CTCAG 1 cut(s) 771
BspFNI CGCG 3 cut(s) 462, 528, 1081
BspOI GCTAGC 2 cut(s) 630, 732
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 986
BsrBI CCGCTC 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 2 cut(s) 341, 1067
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 3 cut(s) 295, 952, 1000
BssSI CACGAG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 5 cut(s) 77, 296, 311, 954, 1002
Bst4CI ACNGT 4 cut(s) 182, 703, 911, 1102
Bst6I CTCTTC 1 cut(s) 675
BstAFI CTTAAG 1 cut(s) 986
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 7 cut(s) 305, 379, 383, 628, 730, 1079, 1083
BstDEI CTNAG 3 cut(s) 417, 429, 779
BstF5I GGATG 2 cut(s) 376, 671
BstFNI CGCG 3 cut(s) 462, 528, 1081
BstHHI GCGC 1 cut(s) 941
BstMAI GTCTC 1 cut(s) 775
BstMCI CGRYCG 1 cut(s) 1132
BstMWI GCNNNNNNNGC 4 cut(s) 611, 617, 623, 725
BstNI CCWGG 5 cut(s) 77, 296, 311, 954, 1002
BstUI CGCG 3 cut(s) 462, 528, 1081
BstV1I GCAGC 3 cut(s) 604, 626, 706
BstV2I GAAGAC 5 cut(s) 30, 72, 117, 204, 243
BtsCI GGATG 2 cut(s) 376, 671
BtsIMutI CAGTG 2 cut(s) 907, 1060
Cac8I GCNNGC 7 cut(s) 305, 379, 383, 628, 730, 1079, 1083
CaiI CAGNNNCTG 2 cut(s) 620, 707
CfoI GCGC 1 cut(s) 941
Cfr10I RCCGGY 1 cut(s) 377
CseI GACGC 1 cut(s) 573
Csp6I GTAC 5 cut(s) 351, 494, 578, 855, 1068
CviAII CATG 2 cut(s) 825, 858
CviQI GTAC 5 cut(s) 351, 494, 578, 855, 1068
DdeI CTNAG 3 cut(s) 417, 429, 779
DraIII CACNNNGTG 1 cut(s) 644
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
EciI GGCGGA 1 cut(s) 155
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 8 cut(s) 79, 133, 163, 313, 319, 479, 590, 692
Eco57I CTGAAG 3 cut(s) 191, 377, 627
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 5 cut(s) 75, 294, 309, 952, 1000
EcoRV GATATC 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 671
Esp3I CGTCTC 1 cut(s) 775
FaeI CATG 2 cut(s) 828, 861
FaqI GGGAC 1 cut(s) 914
FatI CATG 2 cut(s) 824, 857
FauI CCCGC 2 cut(s) 390, 1086
FauNDI CATATG 1 cut(s) 897
Fnu4HI GCNGC 8 cut(s) 44, 176, 257, 301, 612, 615, 618, 720
FokI GGATG 2 cut(s) 383, 678
FseI GGCCGGCC 1 cut(s) 381
Fsp4HI GCNGC 8 cut(s) 44, 176, 257, 301, 612, 615, 618, 720
FspBI CTAG 6 cut(s) 72, 204, 602, 627, 729, 965
GlaI GCGC 1 cut(s) 940
GluI GCNGC 8 cut(s) 44, 176, 257, 301, 612, 615, 618, 720
GsaI CCCAGC 1 cut(s) 140
HapII CCGG 6 cut(s) 131, 317, 323, 378, 545, 932
HgaI GACGC 1 cut(s) 573
HhaI GCGC 1 cut(s) 941
Hin1II CATG 2 cut(s) 828, 861
Hin6I GCGC 1 cut(s) 939
HinP1I GCGC 1 cut(s) 939
HinfI GANTC 3 cut(s) 239, 364, 773
HpaII CCGG 6 cut(s) 131, 317, 323, 378, 545, 932
Hpy166II GTNNAC 4 cut(s) 124, 479, 563, 983
Hpy188I TCNGA 4 cut(s) 244, 280, 780, 1057
Hpy188III TCNNGA 5 cut(s) 368, 593, 695, 770, 920
Hpy8I GTNNAC 4 cut(s) 124, 479, 563, 983
HpyAV CCTTC 5 cut(s) 352, 430, 550, 877, 929
HpyCH4III ACNGT 4 cut(s) 182, 703, 911, 1102
HpyCH4IV ACGT 5 cut(s) 489, 573, 675, 784, 844
HpyCH4V TGCA 4 cut(s) 669, 794, 907, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 611, 617, 623, 725
HpyF3I CTNAG 3 cut(s) 417, 429, 779
HpySE526I ACGT 5 cut(s) 489, 573, 675, 784, 844
Hsp92II CATG 2 cut(s) 828, 861
HspAI GCGC 1 cut(s) 939
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 3 cut(s) 604, 626, 706
LweI GCATC 3 cut(s) 656, 950, 1061
MaeI CTAG 6 cut(s) 72, 204, 602, 627, 729, 965
MaeII ACGT 5 cut(s) 489, 573, 675, 784, 844
MaeIII GTNAC 3 cut(s) 103, 229, 463
MbiI CCGCTC 1 cut(s) 385
MboII GAAGA 6 cut(s) 35, 77, 122, 209, 248, 692
MluCI AATT 2 cut(s) 833, 1009
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 9 cut(s) 286, 367, 433, 499, 547, 561, 627, 663, 729
Mph1103I ATGCAT 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 5 cut(s) 335, 486, 570, 672, 987
MspA1I CMGCKG 2 cut(s) 617, 1134
MspCI CTTAAG 1 cut(s) 986
MspI CCGG 6 cut(s) 131, 317, 323, 378, 545, 932
MvaI CCWGG 5 cut(s) 77, 296, 311, 954, 1002
MvnI CGCG 3 cut(s) 462, 528, 1081
MwoI GCNNNNNNNGC 4 cut(s) 611, 617, 623, 725
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 5 cut(s) 131, 317, 323, 545, 932
NdeI CATATG 1 cut(s) 897
NgoMIV GCCGGC 1 cut(s) 377
NheI GCTAGC 2 cut(s) 626, 728
NlaIII CATG 2 cut(s) 828, 861
NmuCI GTSAC 3 cut(s) 103, 229, 463
NsiI ATGCAT 1 cut(s) 671
NspV TTCGAA 1 cut(s) 195
PdiI GCCGGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 364, 773
Pfl23II CGTACG 1 cut(s) 350
PflFI GACNNNGTC 1 cut(s) 926
PfoI TCCNGGA 2 cut(s) 543, 930
PkrI GCNGC 8 cut(s) 45, 177, 258, 302, 613, 616, 619, 721
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PpuMI RGGWCCY 1 cut(s) 313
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 5 cut(s) 75, 294, 309, 952, 1000
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 5 cut(s) 75, 294, 309, 952, 1000
PspLI CGTACG 1 cut(s) 350
PspPPI RGGWCCY 1 cut(s) 313
PstNI CAGNNNCTG 2 cut(s) 620, 707
PsyI GACNNNGTC 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 617
RigI GGCCGGCC 1 cut(s) 381
RsaI GTAC 5 cut(s) 352, 495, 579, 856, 1069
RsaNI GTAC 5 cut(s) 351, 494, 578, 855, 1068
SaqAI TTAA 5 cut(s) 335, 486, 570, 672, 987
SatI GCNGC 8 cut(s) 44, 176, 257, 301, 612, 615, 618, 720
SchI GAGTC 1 cut(s) 233
SfaNI GCATC 3 cut(s) 656, 950, 1061
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 8 cut(s) 79, 133, 163, 313, 319, 479, 590, 692
SmlI CTYRAG 2 cut(s) 986, 1031
SmoI CTYRAG 2 cut(s) 986, 1031
SpeI ACTAGT 1 cut(s) 964
Sse9I AATT 2 cut(s) 833, 1009
SspMI CTAG 6 cut(s) 72, 204, 602, 627, 729, 965
TaaI ACNGT 4 cut(s) 182, 703, 911, 1102
TaiI ACGT 5 cut(s) 492, 576, 678, 787, 847
TaqI TCGA 3 cut(s) 195, 840, 921
TasI AATT 2 cut(s) 833, 1009
TatI WGTACW 3 cut(s) 493, 577, 854
TauI GCSGC 5 cut(s) 46, 178, 259, 303, 614
TfiI GAWTC 2 cut(s) 364, 773
Tru1I TTAA 5 cut(s) 335, 486, 570, 672, 987
Tru9I TTAA 5 cut(s) 335, 486, 570, 672, 987
TscAI CASTG 2 cut(s) 914, 1067
TseFI GTSAC 3 cut(s) 103, 229, 463
TseI GCWGC 3 cut(s) 614, 617, 719
Tsp45I GTSAC 3 cut(s) 103, 229, 463
TspDTI ATGAA 3 cut(s) 826, 1022, 1033
TspGWI ACGGA 3 cut(s) 122, 248, 632
TspRI CASTG 2 cut(s) 914, 1067
Tth111I GACNNNGTC 1 cut(s) 926
Vha464I CTTAAG 1 cut(s) 986
VpaK11BI GGWCC 8 cut(s) 79, 133, 163, 313, 319, 479, 590, 692
XapI RAATTY 1 cut(s) 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 6 cut(s) 72, 204, 602, 627, 729, 965
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.