RchiOBHm_Chr5g0026211

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012769
Physical Location & Seq
Forward (+)
20245734 .. 20247076
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGGTGGACTTAGGCCTATCCAAAGTACCTGAACAATACATACAACCACCTCATGAGCGTATACTGAAGCTTAATGCAAGCTCACTAAATGAGCTCAGAGCCTCAGACCAATCGATCTGTTCTTGGGTTCTTCCAAGTTGTGAACCATGGCGGCATGGCCTGCCTGTGGAGTTGCTGGAGTCTCTGAAAGATGCTGCAAACAATTTCTTCGAGCAAGCGCCAGAGAAGAAAGCTGCTTACCGGAAAGGTGTGAGCCCGAGCCCGTACGTGAAGTATGGAACGAGTTTTGTGCCGGAGAAAGAGAAGGCTTTGGGGTGGAAGGACTATGTTAGCATGGTGTACTCCAGTGATGCTGATGCTCTCAGTCACTGGCCTAACGAGTGCAATACCTTAAAAGAATCAATGCATATGGTAAAGAAAATAGTGAAAACTTTGATTGAGAAGCTCGGAGTGACACTTGAGGACTCAAAACTAGATGGACTCGTTGGGTTAAAGATGGTGAACATGGTATATCCAAAAAAAATAAAGATGGTGAGCATGAACTTTTACCCAACATGCCCTAATCCGGAGCTCACTGTCGGAGTTGGGCGTCACTCGGACATGGGCACCTTAACCGTTTTACTGCAAGATGGAATTGGTGGTTTGTATGTGAAGGTTGAAGAAGACATGAATGCTGGAAAGAAAGGAGAGTGGATCGAGATCCCTCCTATCCCTGGAGCCTTGATACTTGGTAATGGGAGATATAAAAGTGCCGAATACAGAGTTCGAACTACAAACGCTCAATCAAGAGTGTCTGTTCCGATATTTACCATTCCAAATCCAACAGAAATAATTGGGCCGCTGCCGGAGGTGGTGGCGCGTGATGGAGTGGCTCGTTACAGTGACATGGTTTTTGGAGATTATATGAACAACTTTTTCGGCAATGCACATGAGGGCAAGAAGTCTCATGATTATGCCCAGATTAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01110 map00940 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

323

Amino Acids

35.99

Weight (kDa)

6.67

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 52 - 126 8.1e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 177 - 270 2.2e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12900
fragaria_vesca FvH4_3g15580
malus_domestica MD00G1188900.v1.1 MD05G1221300.v1.1
prunus_persica Prupe.4G138300_v2.0.a1
pyrus_communis pycom05g20470
rosa_chinensis RchiOBHm_Chr5g0026211
rosa_laevigata RLG00000032912
rosa_multiflora Rmu_co8420971.1_g000001 Rmu_sc0002909.1_g000002 Rmu_sc0005061.1_g000001
rosa_roxburghii Rroxscaffold_1G00053100
rosa_rugosa Rorug05G0091600
rosa_samantha Rh5AG184500 Rh5BG181400 Rh5CG200500 Rh5DG183100
rosa_wichuraiana Rw5G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 605
AccI GTMKAC 1 cut(s) 61
AccII CGCG 1 cut(s) 859
AccIII TCCGGA 1 cut(s) 565
AciI CCGC 2 cut(s) 151, 839
AclWI GGATC 2 cut(s) 694, 701
AcuI CTGAAG 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 589
AfaI GTAC 3 cut(s) 27, 266, 341
AfiI CCNNNNNNNGG 3 cut(s) 166, 565, 713
AgsI TTSAA 1 cut(s) 659
AjnI CCWGG 1 cut(s) 712
AluBI AGCT 6 cut(s) 70, 81, 94, 233, 445, 571
AluI AGCT 6 cut(s) 70, 81, 94, 233, 445, 571
Alw21I GWGCWC 2 cut(s) 96, 573
Alw26I GTCTC 2 cut(s) 186, 948
AlwI GGATC 2 cut(s) 694, 701
AlwNI CAGNNNCTG 1 cut(s) 369
Ama87I CYCGRG 1 cut(s) 256
Aor13HI TCCGGA 1 cut(s) 565
AoxI GGCC 4 cut(s) 13, 157, 371, 836
ApeKI GCWGC 3 cut(s) 194, 233, 841
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
AseI ATTAAT 1 cut(s) 963
AspLEI GCGC 2 cut(s) 220, 859
AspS9I GGNCC 1 cut(s) 836
AsuHPI GGTGA 2 cut(s) 511, 544
AsuII TTCGAA 1 cut(s) 766
AvaI CYCGRG 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 4 cut(s) 96, 257, 263, 573
BbsI GAAGAC 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 96, 573
BbvI GCAGC 3 cut(s) 181, 220, 828
BccI CCATC 5 cut(s) 470, 490, 523, 623, 857
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 1 cut(s) 714
BcoDI GTCTC 2 cut(s) 186, 948
BfaI CTAG 1 cut(s) 473
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 5 cut(s) 152, 195, 234, 839, 842
BlsI GCNGC 5 cut(s) 153, 196, 235, 840, 843
Bme1390I CCNGG 1 cut(s) 714
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 836
BmiI GGNNCC 2 cut(s) 607, 718
BmrFI CCNGG 1 cut(s) 714
BmsI GCATC 3 cut(s) 181, 340, 346
BpiI GAAGAC 1 cut(s) 669
BpmI CTGGAG 3 cut(s) 197, 328, 735
Bpu14I TTCGAA 1 cut(s) 766
BpuEI CTTGAG 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 698
BsaHI GRCGYC 1 cut(s) 589
BsaJI CCNNGG 2 cut(s) 146, 712
BsaWI WCCGGW 2 cut(s) 240, 565
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 565, 713
Bse1I ACTGG 2 cut(s) 345, 374
Bse3DI GCAATG 1 cut(s) 928
Bse8I GATNNNNATC 1 cut(s) 698
BseAI TCCGGA 1 cut(s) 565
BseBI CCWGG 1 cut(s) 714
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 146, 712
BseJI GATNNNNATC 1 cut(s) 698
BseLI CCNNNNNNNGG 3 cut(s) 166, 565, 713
BseMI GCAATG 1 cut(s) 928
BseMII CTCAG 3 cut(s) 109, 117, 376
BseNI ACTGG 2 cut(s) 345, 374
BseSI GKGCMC 1 cut(s) 608
BseXI GCAGC 3 cut(s) 181, 220, 828
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 4 cut(s) 15, 159, 373, 838
BshNI GGYRCC 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 96, 573
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 4 cut(s) 241, 293, 566, 845
BsiWI CGTACG 1 cut(s) 264
BslI CCNNNNNNNGG 3 cut(s) 166, 565, 713
BsmAI GTCTC 2 cut(s) 186, 948
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 4 cut(s) 15, 159, 373, 838
BsoBI CYCGRG 1 cut(s) 256
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 5 cut(s) 96, 257, 263, 573, 608
Bsp13I TCCGGA 1 cut(s) 565
Bsp143I GATC 3 cut(s) 114, 693, 699
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 2 cut(s) 151, 839
BspANI GGCC 4 cut(s) 15, 159, 373, 838
BspCNI CTCAG 3 cut(s) 108, 116, 375
BspDI ATCGAT 1 cut(s) 113
BspEI TCCGGA 1 cut(s) 565
BspFNI CGCG 1 cut(s) 859
BspHI TCATGA 2 cut(s) 52, 946
BspLI GGNNCC 2 cut(s) 607, 718
BspPI GGATC 2 cut(s) 694, 701
BspT104I TTCGAA 1 cut(s) 766
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 928
BsrI ACTGG 2 cut(s) 345, 374
BssECI CCNNGG 2 cut(s) 146, 712
BssMI GATC 3 cut(s) 114, 693, 699
BssNAI GTATAC 1 cut(s) 62
BssNI GRCGYC 1 cut(s) 589
BssT1I CCWWGG 1 cut(s) 146
Bst1107I GTATAC 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 577, 616, 881
BstACI GRCGYC 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 160
BstBAI YACGTR 1 cut(s) 268
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 3 cut(s) 79, 161, 216
BstDEI CTNAG 4 cut(s) 10, 95, 103, 362
BstDSI CCRYGG 1 cut(s) 146
BstFNI CGCG 1 cut(s) 859
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 859
BstKTI GATC 3 cut(s) 117, 696, 702
BstMAI GTCTC 2 cut(s) 186, 948
BstMBI GATC 3 cut(s) 114, 693, 699
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNI CCWGG 1 cut(s) 714
BstNSI RCATGY 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 608
BstUI CGCG 1 cut(s) 859
BstV1I GCAGC 3 cut(s) 181, 220, 828
BstV2I GAAGAC 1 cut(s) 669
BstX2I RGATCY 1 cut(s) 699
BstYI RGATCY 1 cut(s) 699
BstZ17I GTATAC 1 cut(s) 62
Bsu15I ATCGAT 1 cut(s) 113
BsuRI GGCC 4 cut(s) 15, 159, 373, 838
BsuTUI ATCGAT 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 146
BtsIMutI CAGTG 4 cut(s) 352, 367, 573, 886
Cac8I GCNNGC 3 cut(s) 79, 161, 216
CaiI CAGNNNCTG 1 cut(s) 369
CciI TCATGA 2 cut(s) 52, 946
CfoI GCGC 2 cut(s) 220, 859
Cfr13I GGNCC 1 cut(s) 836
ClaI ATCGAT 1 cut(s) 113
CseI GACGC 1 cut(s) 578
Csp6I GTAC 3 cut(s) 26, 265, 340
CviQI GTAC 3 cut(s) 26, 265, 340
DdeI CTNAG 4 cut(s) 10, 95, 103, 362
DpnI GATC 3 cut(s) 116, 695, 701
DpnII GATC 3 cut(s) 114, 693, 699
Ecl136II GAGCTC 2 cut(s) 94, 571
Eco130I CCWWGG 1 cut(s) 146
Eco147I AGGCCT 1 cut(s) 15
Eco24I GRGCYC 4 cut(s) 96, 257, 263, 573
Eco53kI GAGCTC 2 cut(s) 94, 571
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 2 cut(s) 94, 571
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 4 cut(s) 96, 257, 263, 573
ErhI CCWWGG 1 cut(s) 146
FauNDI CATATG 1 cut(s) 408
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 152, 195, 234, 839, 842
FriOI GRGCYC 4 cut(s) 96, 257, 263, 573
Fsp4HI GCNGC 5 cut(s) 152, 195, 234, 839, 842
FspBI CTAG 1 cut(s) 473
GlaI GCGC 2 cut(s) 219, 858
GluI GCNGC 5 cut(s) 152, 195, 234, 839, 842
GsuI CTGGAG 3 cut(s) 197, 328, 735
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 4 cut(s) 15, 159, 373, 838
HapII CCGG 4 cut(s) 241, 293, 566, 845
HgaI GACGC 1 cut(s) 578
HhaI GCGC 2 cut(s) 220, 859
Hin1I GRCGYC 1 cut(s) 589
Hin6I GCGC 2 cut(s) 218, 857
HinP1I GCGC 2 cut(s) 218, 857
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 4 cut(s) 179, 398, 464, 480
HpaII CCGG 4 cut(s) 241, 293, 566, 845
HphI GGTGA 2 cut(s) 511, 544
Hpy166II GTNNAC 5 cut(s) 7, 62, 143, 340, 502
Hpy188I TCNGA 7 cut(s) 98, 106, 186, 449, 581, 598, 801
Hpy188III TCNNGA 5 cut(s) 53, 566, 697, 786, 947
Hpy8I GTNNAC 5 cut(s) 7, 62, 143, 340, 502
HpyAV CCTTC 3 cut(s) 298, 313, 646
HpyCH4III ACNGT 3 cut(s) 577, 616, 881
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 6 cut(s) 77, 197, 384, 406, 625, 926
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 4 cut(s) 10, 95, 103, 362
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 589
HspAI GCGC 2 cut(s) 218, 857
Kpn2I TCCGGA 1 cut(s) 565
Kzo9I GATC 3 cut(s) 114, 693, 699
LmnI GCTCC 2 cut(s) 568, 716
Lsp1109I GCAGC 3 cut(s) 181, 220, 828
LweI GCATC 3 cut(s) 181, 340, 346
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 5 cut(s) 365, 451, 590, 875, 881
MalI GATC 3 cut(s) 116, 695, 701
MboI GATC 3 cut(s) 114, 693, 699
MboII GAAGA 5 cut(s) 122, 199, 238, 671, 674
MflI RGATCY 1 cut(s) 699
MhlI GDGCHC 5 cut(s) 96, 257, 263, 573, 608
MluCI AATT 4 cut(s) 202, 633, 831, 967
MlyI GAGTC 3 cut(s) 188, 458, 474
MmeI TCCRAC 2 cut(s) 559, 845
MnlI CCTC 6 cut(s) 60, 112, 454, 714, 841, 925
Mph1103I ATGCAT 1 cut(s) 408
MroI TCCGGA 1 cut(s) 565
MseI TTAA 5 cut(s) 72, 392, 491, 611, 963
MslI CAYNNNNRTG 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 4 cut(s) 241, 293, 566, 845
MspR9I CCNGG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 1 cut(s) 160
NcoI CCATGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 408
NdeII GATC 3 cut(s) 114, 693, 699
NlaIV GGNNCC 2 cut(s) 607, 718
NmuCI GTSAC 4 cut(s) 365, 451, 590, 881
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 1 cut(s) 558
NspV TTCGAA 1 cut(s) 766
PagI TCATGA 2 cut(s) 52, 946
PceI AGGCCT 1 cut(s) 15
PctI GAATGC 1 cut(s) 676
PfeI GAWTC 1 cut(s) 398
Pfl23II CGTACG 1 cut(s) 264
PkrI GCNGC 5 cut(s) 153, 196, 235, 840, 843
PleI GAGTC 3 cut(s) 187, 458, 474
PpsI GAGTC 3 cut(s) 187, 458, 474
Ppu21I YACGTR 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 963
Psp124BI GAGCTC 2 cut(s) 96, 573
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspLI CGTACG 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 607, 718
PspPI GGNCC 1 cut(s) 836
PsrI GAACNNNNNNTAC 2 cut(s) 24, 56
PstNI CAGNNNCTG 1 cut(s) 369
PsuI RGATCY 1 cut(s) 699
RsaI GTAC 3 cut(s) 27, 266, 341
RsaNI GTAC 3 cut(s) 26, 265, 340
RseI CAYNNNNRTG 1 cut(s) 951
SacI GAGCTC 2 cut(s) 96, 573
SaqAI TTAA 5 cut(s) 72, 392, 491, 611, 963
SatI GCNGC 5 cut(s) 152, 195, 234, 839, 842
Sau3AI GATC 3 cut(s) 114, 693, 699
Sau96I GGNCC 1 cut(s) 836
SchI GAGTC 3 cut(s) 188, 458, 474
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 5 cut(s) 96, 257, 263, 573, 608
SfaNI GCATC 3 cut(s) 181, 340, 346
SfuI TTCGAA 1 cut(s) 766
SmiMI CAYNNNNRTG 1 cut(s) 951
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 4 cut(s) 202, 633, 831, 967
SseBI AGGCCT 1 cut(s) 15
SsiI CCGC 2 cut(s) 151, 839
SspMI CTAG 1 cut(s) 473
SstI GAGCTC 2 cut(s) 96, 573
StuI AGGCCT 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 712
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 3 cut(s) 577, 616, 881
TaiI ACGT 1 cut(s) 270
TaqI TCGA 4 cut(s) 113, 210, 696, 766
TasI AATT 4 cut(s) 202, 633, 831, 967
TatI WGTACW 1 cut(s) 339
TauI GCSGC 2 cut(s) 154, 841
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 5 cut(s) 72, 392, 491, 611, 963
Tru9I TTAA 5 cut(s) 72, 392, 491, 611, 963
TscAI CASTG 4 cut(s) 352, 374, 580, 886
TseFI GTSAC 4 cut(s) 365, 451, 590, 881
TseI GCWGC 3 cut(s) 194, 233, 841
Tsp45I GTSAC 4 cut(s) 365, 451, 590, 881
TspDTI ATGAA 3 cut(s) 554, 683, 920
TspRI CASTG 4 cut(s) 352, 374, 580, 886
VspI ATTAAT 1 cut(s) 963
XceI RCATGY 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 1 cut(s) 473
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.