RchiOBHm_Chr4g0402811

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008309
Physical Location & Seq
Forward (+)
21656875 .. 21657306
432 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGCCTCTCACTTCCATTGTAATGGCTACCCATCACATGAAGTCCACTCATTTCGATAACTTTGATATCGATGACAAGGCCAATGAGGTGGCTTGCCGGATTGTTGCCTCGGATACTGAGCTAGAGAAGAGGATGAAATTTGAGACTCGTGATATCATGAAAGTTCGGGAGAGGCTTGCCGAGTATGATTGCATATTTTTGGTAGCTCTGGTGGGAATGTACAAAGAAGACAAGGTGAAGATTCTCAGGCATATTAGGAAGTATATGAAGGAAGGAAGCTTTTTGCTTGTGAGGAGTGCAAAAGGGTCTAGGGCATTCTTGTACCCTGTTATTCAGGAGCATGACTTGCTGGGCTTTGAGCTTCTAACAATCTTCCATCCCAATCAGTGGCGGACGCAGGAATATATATCTCTGGGGGCAGGTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

143

Amino Acids

16.77

Weight (kDa)

8.52

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAS PF03059 1 - 129 3.3e-47 Nicotianamine synthase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 387
AciI CCGC 1 cut(s) 391
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 2 cut(s) 221, 323
AfiI CCNNNNNNNGG 1 cut(s) 387
AgsI TTSAA 1 cut(s) 427
AluBI AGCT 4 cut(s) 121, 206, 279, 361
AluI AGCT 4 cut(s) 121, 206, 279, 361
Alw26I GTCTC 1 cut(s) 137
AoxI GGCC 1 cut(s) 78
ApoI RAATTY 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 247
BauI CACGAG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 234
BccI CCATC 2 cut(s) 39, 384
BciVI GTATCC 1 cut(s) 106
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 2 cut(s) 122, 309
BfuAI ACCTGC 1 cut(s) 410
BfuI GTATCC 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 387
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 138, 376
BseLI CCNNNNNNNGG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 108, 259
BseRI GAGGAG 1 cut(s) 307
BseYI CCCAGC 1 cut(s) 349
BshFI GGCC 1 cut(s) 80
BshVI ATCGAT 1 cut(s) 69
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 1 cut(s) 80
Bsp1407I TGTACA 1 cut(s) 219
BspACI CCGC 1 cut(s) 391
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 109, 258
BspDI ATCGAT 1 cut(s) 69
BspHI TCATGA 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 108
BssSI CACGAG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 147
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 346
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 94, 177
BstDEI CTNAG 2 cut(s) 117, 245
BstF5I GGATG 2 cut(s) 138, 376
BstMAI GTCTC 1 cut(s) 137
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstV2I GAAGAC 1 cut(s) 234
BstXI CCANNNNNNTGG 2 cut(s) 22, 88
Bsu15I ATCGAT 1 cut(s) 69
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 80
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 2 cut(s) 138, 376
BtsIMutI CAGTG 1 cut(s) 392
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 2 cut(s) 94, 177
CciI TCATGA 1 cut(s) 156
ClaI ATCGAT 1 cut(s) 69
CseI GACGC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 220, 322
CviAII CATG 3 cut(s) 37, 157, 341
CviJI RGCY 9 cut(s) 26, 80, 92, 121, 175, 206, 279, 354, 361
CviKI_1 RGCY 9 cut(s) 26, 80, 92, 121, 175, 206, 279, 354, 361
CviQI GTAC 2 cut(s) 220, 322
DdeI CTNAG 2 cut(s) 117, 245
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 406
Eco32I GATATC 2 cut(s) 67, 154
EcoRV GATATC 2 cut(s) 67, 154
FaeI CATG 3 cut(s) 40, 160, 344
FatI CATG 3 cut(s) 36, 156, 340
FokI GGATG 2 cut(s) 145, 363
FspBI CTAG 2 cut(s) 122, 309
GsaI CCCAGC 1 cut(s) 353
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 403
Hin1II CATG 3 cut(s) 40, 160, 344
HindIII AAGCTT 1 cut(s) 277
HinfI GANTC 2 cut(s) 145, 241
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 4 cut(s) 149, 157, 167, 335
Hpy8I GTNNAC 1 cut(s) 45
HpyAV CCTTC 2 cut(s) 262, 266
HpyCH4V TGCA 2 cut(s) 192, 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 2 cut(s) 117, 245
Hsp92II CATG 3 cut(s) 40, 160, 344
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 9 cut(s) 110, 194, 232, 320, 335, 339, 383, 398, 405
MaeI CTAG 2 cut(s) 122, 309
MboII GAAGA 4 cut(s) 139, 239, 250, 364
MluCI AATT 1 cut(s) 137
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 6 cut(s) 15, 79, 118, 123, 165, 285
MslI CAYNNNNRTG 1 cut(s) 20
MspI CCGG 1 cut(s) 97
Mva1269I GAATGC 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 346
NlaIII CATG 3 cut(s) 40, 160, 344
NmeAIII GCCGAG 1 cut(s) 205
PagI TCATGA 1 cut(s) 156
PctI GAATGC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 241
PflMI CCANNNNNTGG 1 cut(s) 387
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PspFI CCCAGC 1 cut(s) 349
RsaI GTAC 2 cut(s) 221, 323
RsaNI GTAC 2 cut(s) 220, 322
RseI CAYNNNNRTG 1 cut(s) 20
SchI GAGTC 1 cut(s) 139
SetI ASST 7 cut(s) 90, 123, 208, 237, 281, 363, 424
SmiMI CAYNNNNRTG 1 cut(s) 20
Sse9I AATT 1 cut(s) 137
SsiI CCGC 1 cut(s) 391
SspMI CTAG 2 cut(s) 122, 309
TaqI TCGA 2 cut(s) 54, 69
TasI AATT 1 cut(s) 137
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 241
TscAI CASTG 1 cut(s) 392
TspDTI ATGAA 4 cut(s) 53, 149, 173, 281
TspRI CASTG 1 cut(s) 392
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 1 cut(s) 137
XspI CTAG 2 cut(s) 122, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.