RchiOBHm_Chr4g0437291

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004856
Physical Location & Seq
Reverse (-)
60434178 .. 60436559
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGCATTTGCAATGGAGGCCTGTAGCTCTGTTCGGAGGGAGCATTTACTCCCACACTCCAGGCCAGAGTCCAAAAAATCAACAATGGCACCACAGATATATGCGTGCGATTGTGCATTTAGCAGTATGCATGTCGAAATTGTACCTTTCTTTTCTCACGGTTATTACTTTTCTTCTCTGCATAAAAATCTTTAAAACGTATGTACTTATTGTCAATACACATGTCGTTGATCATCATCAACAACAACATCATCATATATCTTCCTCTTCTTCTTCACCATCTCATTTTCAAGGGGATTACGTGAACAATGGATTTCTCATGGTCTCATCCAATGGAGGATTGAATCAAATGCGAGCGGGCATTTGTGACATGGTAGCTATTGCAAGGTTTTTGAATGTCACGCTTATAGTTCCCGAGTTGGATAAAACCTCCTTTTGGAATGATCGGAGTCAGTTTGAAGATATATTCGACGTGGATCACTTCATAGCATCATTGAGCGATGAAGTCCGTATATTGAAAGTTCTGCCCCAGAAGCAGAGAAGAAGAATAGAAATGGCTTCCCTCTATTCCATGCCCCCTGCTAGTTGGTCTAATATGACATTTTACTACAACCGGGTTCTTCCTCAGATAAAGAAGTATGAAGTACTGCATTTCACTAAAACTGATACAAGACTTGCCAATAATGGGATCCCAGTGGAAGTTCAGAAAATGCGCTGCAAAGCAAATTATGAAGCCCTGAGGTTCACTCCCCCAATTGAGGAATTGGGGAAGAAGATTGTAAGGATTCTGAGAGAAAGGGGCCCGTTCTTAGTTCTTCATCTCAGATATGAAATGGACATGTTAGCCTTTTCGGGTTGCACTGAAGGCTGCGATGACAAAGAAATCGAAGAATTGACAAATATGAGATATGCTTATCCATGGTGGAAAGAGAAAGTAATTAATTCCACCCAAAAAAGAAAATCTGGGAAATGCCCATTAACTCCCGAGGAGACTGCTCTTGCACTGAGGGCATTGGATATCGATCCTAGTATCCAAGTATACATAGCTGCAGGTGATATTTATGGGGGAGAAAGACGAATGGCATCTCTAAGATTAGCCTTTCCACACATAGTTAAGAAAGAAACATTGCTGGAAGCCTCAGACCTTGAGCCCTTCCGAAACCATTCGAACCAAATGGCTGCATTGGATTACATTGTGTCACTGGAAAGTGATATCTTCGCTCCAACTTATGGAGGAAACATGGCAAGAGTTGTCGAAGGCCATAGACGGTACTTAGGTTTCAGGACAACAATTCGTCTGGACAGAATGCTTCTAGTGAATTTGATAGACCAGTACAAGAAGGGTAGACTGAGCTGGGATGAATTTTCCCAGGCAGTGAAAACAGGACATGCCGATCGCATGGGTAGCCCTACACAAAGATTGGAGATTCCTGGTAAACCCAAGGAAGAGGAATACTTCTATAACAATCCACAGGAATGTTTACCACCAATTTCTACAAAACCAAAGAGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

504

Amino Acids

58.22

Weight (kDa)

9.1

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 105 - 425 3.3e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1040
Acc36I ACCTGC 1 cut(s) 1040
AccB1I GGYRCC 1 cut(s) 87
AccB7I CCANNNNNTGG 1 cut(s) 1229
AccBSI CCGCTC 1 cut(s) 356
AccI GTMKAC 2 cut(s) 1038, 1345
AciI CCGC 1 cut(s) 356
AclWI GGATC 4 cut(s) 483, 682, 695, 1016
AcsI RAATTY 2 cut(s) 1318, 1361
AcuI CTGAAG 1 cut(s) 882
AfaI GTAC 5 cut(s) 143, 204, 645, 1271, 1334
AfiI CCNNNNNNNGG 4 cut(s) 435, 684, 757, 1229
AflIII ACRYGT 2 cut(s) 220, 837
AgsI TTSAA 5 cut(s) 290, 343, 394, 458, 517
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 3 cut(s) 58, 1368, 1429
AluBI AGCT 4 cut(s) 26, 377, 1046, 1353
AluI AGCT 4 cut(s) 26, 377, 1046, 1353
Alw26I GTCTC 2 cut(s) 328, 983
AlwI GGATC 4 cut(s) 483, 682, 695, 1016
Ama87I CYCGRG 2 cut(s) 413, 983
AoxI GGCC 4 cut(s) 17, 61, 799, 1258
ApaI GGGCCC 1 cut(s) 803
ApeKI GCWGC 4 cut(s) 714, 867, 1046, 1178
ApoI RAATTY 2 cut(s) 1318, 1361
AseI ATTAAT 1 cut(s) 939
Asp700I GAANNNNTTC 1 cut(s) 1162
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 799, 800
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 267, 1064
AsuII TTCGAA 1 cut(s) 1166
AvaI CYCGRG 2 cut(s) 413, 983
AxyI CCTNAGG 1 cut(s) 737
BaeGI GKGCMC 1 cut(s) 803
BaeI ACNNNNGTAYC 2 cut(s) 1261, 1294
BamHI GGATCC 1 cut(s) 687
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 2 cut(s) 803, 1152
BbvI GCAGC 4 cut(s) 701, 854, 1033, 1165
BccI CCATC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 3 cut(s) 60, 1370, 1431
BciVI GTATCC 1 cut(s) 1040
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 2 cut(s) 328, 983
BfaI CTAG 3 cut(s) 582, 1026, 1313
BfmI CTRYAG 2 cut(s) 21, 1047
BfuAI ACCTGC 1 cut(s) 1040
BfuI GTATCC 1 cut(s) 1040
BisI GCNGC 4 cut(s) 715, 868, 1047, 1179
BlsI GCNGC 4 cut(s) 716, 869, 1048, 1180
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 4 cut(s) 60, 614, 1370, 1431
BmeT110I CYCGRG 2 cut(s) 413, 983
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 2 cut(s) 799, 800
BmiI GGNNCC 4 cut(s) 89, 689, 800, 801
BmrFI CCNGG 4 cut(s) 60, 614, 1370, 1431
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 2 cut(s) 497, 1091
BmuI ACTGGG 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 730, 762
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 1166
BpuEI CTTGAG 1 cut(s) 1166
BpuMI CCSGG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 1020
BsaAI YACGTR 1 cut(s) 301
BsaBI GATNNNNATC 2 cut(s) 234, 1020
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 4 cut(s) 917, 984, 1368, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 435, 684, 757, 1229
Bse1I ACTGG 3 cut(s) 692, 1206, 1330
Bse21I CCTNAGG 1 cut(s) 737
Bse3DI GCAATG 2 cut(s) 17, 1124
Bse8I GATNNNNATC 2 cut(s) 234, 1020
BseBI CCWGG 3 cut(s) 60, 1370, 1431
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 4 cut(s) 917, 984, 1368, 1440
BseGI GGATG 2 cut(s) 326, 1363
BseJI GATNNNNATC 2 cut(s) 234, 1020
BseLI CCNNNNNNNGG 4 cut(s) 435, 684, 757, 1229
BseMI GCAATG 2 cut(s) 17, 1124
BseMII CTCAG 7 cut(s) 638, 728, 779, 835, 995, 1152, 1340
BseNI ACTGG 3 cut(s) 692, 1206, 1330
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 4 cut(s) 701, 854, 1033, 1165
BseYI CCCAGC 1 cut(s) 1353
Bsh1285I CGRYCG 1 cut(s) 1396
BshFI GGCC 4 cut(s) 19, 63, 801, 1260
BshNI GGYRCC 1 cut(s) 87
BshVI ATCGAT 1 cut(s) 1020
BsiEI CGRYCG 1 cut(s) 1396
BsiHKCI CYCGRG 2 cut(s) 413, 983
BsiSI CCGG 1 cut(s) 613
BslI CCNNNNNNNGG 4 cut(s) 435, 684, 757, 1229
BsmAI GTCTC 2 cut(s) 328, 983
BsmI GAATGC 1 cut(s) 1311
BsnI GGCC 4 cut(s) 19, 63, 801, 1260
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 2 cut(s) 413, 983
Bsp119I TTCGAA 1 cut(s) 1166
Bsp120I GGGCCC 1 cut(s) 799
Bsp1286I GDGCHC 2 cut(s) 803, 1152
Bsp143I GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 1 cut(s) 356
BspANI GGCC 4 cut(s) 19, 63, 801, 1260
BspCNI CTCAG 7 cut(s) 637, 729, 780, 834, 996, 1151, 1341
BspDI ATCGAT 1 cut(s) 1020
BspLI GGNNCC 4 cut(s) 89, 689, 800, 801
BspMAI CTGCAG 1 cut(s) 1051
BspMI ACCTGC 1 cut(s) 1040
BspPI GGATC 4 cut(s) 483, 682, 695, 1016
BspT104I TTCGAA 1 cut(s) 1166
BspT107I GGYRCC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 356
BsrDI GCAATG 2 cut(s) 17, 1124
BsrI ACTGG 3 cut(s) 692, 1206, 1330
BssECI CCNNGG 4 cut(s) 917, 984, 1368, 1440
BssMI GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
BssNAI GTATAC 1 cut(s) 1039
BssT1I CCWWGG 2 cut(s) 917, 1440
Bst1107I GTATAC 1 cut(s) 1039
Bst2UI CCWGG 3 cut(s) 60, 1370, 1431
Bst4CI ACNGT 2 cut(s) 160, 1269
Bst6I CTCTTC 2 cut(s) 271, 1440
BstBAI YACGTR 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 1166
BstC8I GCNNGC 3 cut(s) 105, 354, 358
BstDSI CCRYGG 1 cut(s) 917
BstF5I GGATG 2 cut(s) 326, 1363
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 6 cut(s) 232, 445, 478, 690, 1024, 1396
BstMAI GTCTC 2 cut(s) 328, 983
BstMBI GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
BstMCI CGRYCG 1 cut(s) 1396
BstMWI GCNNNNNNNGC 5 cut(s) 16, 532, 864, 1007, 1404
BstNI CCWGG 3 cut(s) 60, 1370, 1431
BstNSI RCATGY 4 cut(s) 133, 224, 841, 1391
BstSCI CCNGG 4 cut(s) 58, 612, 1368, 1429
BstSFI CTRYAG 2 cut(s) 21, 1047
BstSLI GKGCMC 1 cut(s) 803
BstV1I GCAGC 4 cut(s) 701, 854, 1033, 1165
BstX2I RGATCY 1 cut(s) 687
BstYI RGATCY 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 1039
Bsu15I ATCGAT 1 cut(s) 1020
Bsu36I CCTNAGG 1 cut(s) 737
BsuI GTATCC 1 cut(s) 1040
BsuRI GGCC 4 cut(s) 19, 63, 801, 1260
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 1 cut(s) 917
BtgZI GCGATG 2 cut(s) 513, 885
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 2 cut(s) 326, 1363
BtsI GCAGTG 1 cut(s) 1380
BtsIMutI CAGTG 5 cut(s) 699, 858, 1001, 1199, 1380
BveI ACCTGC 1 cut(s) 1040
Cac8I GCNNGC 3 cut(s) 105, 354, 358
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 799, 800
ClaI ATCGAT 1 cut(s) 1020
Csp6I GTAC 5 cut(s) 142, 203, 644, 1270, 1333
CviQI GTAC 5 cut(s) 142, 203, 644, 1270, 1333
DpnI GATC 6 cut(s) 231, 444, 477, 689, 1023, 1395
DpnII GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
DraI TTTAAA 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 271, 1440
EarI CTCTTC 2 cut(s) 271, 1440
Eco130I CCWWGG 2 cut(s) 917, 1440
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 803, 1152
Eco31I GGTCTC 1 cut(s) 328
Eco32I GATATC 2 cut(s) 1018, 1213
Eco57I CTGAAG 1 cut(s) 882
Eco81I CCTNAGG 1 cut(s) 737
Eco88I CYCGRG 2 cut(s) 413, 983
EcoO109I RGGNCCY 1 cut(s) 799
EcoRII CCWGG 3 cut(s) 58, 1368, 1429
EcoRV GATATC 2 cut(s) 1018, 1213
EcoT14I CCWWGG 2 cut(s) 917, 1440
EcoT22I ATGCAT 2 cut(s) 6, 131
EcoT38I GRGCYC 2 cut(s) 803, 1152
ErhI CCWWGG 2 cut(s) 917, 1440
FauI CCCGC 1 cut(s) 349
FbaI TGATCA 1 cut(s) 229
FblI GTMKAC 2 cut(s) 1038, 1345
Fnu4HI GCNGC 4 cut(s) 715, 868, 1047, 1179
FokI GGATG 2 cut(s) 313, 1370
FriOI GRGCYC 2 cut(s) 803, 1152
Fsp4HI GCNGC 4 cut(s) 715, 868, 1047, 1179
FspBI CTAG 3 cut(s) 582, 1026, 1313
GlaI GCGC 1 cut(s) 713
GluI GCNGC 4 cut(s) 715, 868, 1047, 1179
GsaI CCCAGC 1 cut(s) 1357
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 4 cut(s) 19, 63, 801, 1260
HapII CCGG 1 cut(s) 613
HhaI GCGC 1 cut(s) 714
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HinfI GANTC 5 cut(s) 67, 343, 448, 784, 1426
HpaII CCGG 1 cut(s) 613
HphI GGTGA 2 cut(s) 267, 1064
Hpy166II GTNNAC 6 cut(s) 304, 744, 1039, 1346, 1436, 1481
Hpy188I TCNGA 8 cut(s) 35, 447, 627, 705, 789, 824, 1141, 1157
Hpy188III TCNNGA 4 cut(s) 413, 983, 1282, 1298
Hpy8I GTNNAC 6 cut(s) 304, 744, 1039, 1346, 1436, 1481
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 4 cut(s) 857, 1162, 1250, 1333
HpyCH4III ACNGT 2 cut(s) 160, 1269
HpyCH4IV ACGT 3 cut(s) 197, 300, 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 532, 864, 1007, 1404
HpySE526I ACGT 3 cut(s) 197, 300, 471
HspAI GCGC 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
LmnI GCTCC 2 cut(s) 39, 1225
Lsp1109I GCAGC 4 cut(s) 701, 854, 1033, 1165
LweI GCATC 2 cut(s) 497, 1091
MaeI CTAG 3 cut(s) 582, 1026, 1313
MaeII ACGT 3 cut(s) 197, 300, 471
MaeIII GTNAC 3 cut(s) 365, 397, 1197
MalI GATC 6 cut(s) 231, 444, 477, 689, 1023, 1395
MbiI CCGCTC 1 cut(s) 356
MboI GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 687
MhlI GDGCHC 2 cut(s) 803, 1152
MlyI GAGTC 2 cut(s) 76, 457
MmeI TCCRAC 2 cut(s) 399, 1247
Mph1103I ATGCAT 2 cut(s) 6, 131
MroXI GAANNNNTTC 1 cut(s) 1162
MseI TTAA 4 cut(s) 192, 939, 977, 1113
MslI CAYNNNNRTG 1 cut(s) 1474
MspI CCGG 1 cut(s) 613
MspR9I CCNGG 4 cut(s) 60, 614, 1370, 1431
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 1 cut(s) 1311
MvaI CCWGG 3 cut(s) 60, 1370, 1431
MwoI GCNNNNNNNGC 5 cut(s) 16, 532, 864, 1007, 1404
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 1 cut(s) 917
NdeII GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
NlaIV GGNNCC 4 cut(s) 89, 689, 800, 801
NmuCI GTSAC 3 cut(s) 365, 397, 1197
NsiI ATGCAT 2 cut(s) 6, 131
NspI RCATGY 4 cut(s) 133, 224, 841, 1391
NspV TTCGAA 1 cut(s) 1166
PaqCI CACCTGC 1 cut(s) 1040
PceI AGGCCT 1 cut(s) 19
PciI ACATGT 2 cut(s) 220, 837
PctI GAATGC 1 cut(s) 1311
PdmI GAANNNNTTC 1 cut(s) 1162
PfeI GAWTC 3 cut(s) 343, 784, 1426
PflMI CCANNNNNTGG 1 cut(s) 1229
PkrI GCNGC 4 cut(s) 716, 869, 1048, 1180
Ple19I CGATCG 1 cut(s) 1396
PleI GAGTC 2 cut(s) 75, 456
PpsI GAGTC 2 cut(s) 75, 456
Ppu21I YACGTR 1 cut(s) 301
PscI ACATGT 2 cut(s) 220, 837
PshBI ATTAAT 1 cut(s) 939
Psp6I CCWGG 3 cut(s) 58, 1368, 1429
PspFI CCCAGC 1 cut(s) 1353
PspGI CCWGG 3 cut(s) 58, 1368, 1429
PspN4I GGNNCC 4 cut(s) 89, 689, 800, 801
PspOMI GGGCCC 1 cut(s) 799
PspPI GGNCC 2 cut(s) 799, 800
PstI CTGCAG 1 cut(s) 1051
PsuI RGATCY 1 cut(s) 687
PvuI CGATCG 1 cut(s) 1396
RsaI GTAC 5 cut(s) 143, 204, 645, 1271, 1334
RsaNI GTAC 5 cut(s) 142, 203, 644, 1270, 1333
RseI CAYNNNNRTG 1 cut(s) 1474
SaqAI TTAA 4 cut(s) 192, 939, 977, 1113
SatI GCNGC 4 cut(s) 715, 868, 1047, 1179
Sau3AI GATC 6 cut(s) 229, 442, 475, 687, 1021, 1393
Sau96I GGNCC 2 cut(s) 799, 800
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 2 cut(s) 76, 457
ScrFI CCNGG 4 cut(s) 60, 614, 1370, 1431
SduI GDGCHC 2 cut(s) 803, 1152
SfaNI GCATC 2 cut(s) 497, 1091
SfcI CTRYAG 2 cut(s) 21, 1047
SfuI TTCGAA 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 1474
SmlI CTYRAG 1 cut(s) 1145
SmoI CTYRAG 1 cut(s) 1145
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 1 cut(s) 356
SspMI CTAG 3 cut(s) 582, 1026, 1313
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 58, 612, 1368, 1429
StyI CCWWGG 2 cut(s) 917, 1440
TaaI ACNGT 2 cut(s) 160, 1269
TaiI ACGT 3 cut(s) 200, 303, 474
TaqI TCGA 6 cut(s) 134, 468, 885, 1020, 1166, 1254
TatI WGTACW 3 cut(s) 202, 643, 1332
TfiI GAWTC 3 cut(s) 343, 784, 1426
Tru1I TTAA 4 cut(s) 192, 939, 977, 1113
Tru9I TTAA 4 cut(s) 192, 939, 977, 1113
TscAI CASTG 5 cut(s) 699, 865, 1008, 1206, 1380
TseFI GTSAC 3 cut(s) 365, 397, 1197
TseI GCWGC 4 cut(s) 714, 867, 1046, 1178
Tsp45I GTSAC 3 cut(s) 365, 397, 1197
TspDTI ATGAA 7 cut(s) 472, 516, 654, 744, 806, 843, 1374
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 5 cut(s) 699, 865, 1008, 1206, 1380
Van91I CCANNNNNTGG 1 cut(s) 1229
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 2 cut(s) 1318, 1361
XceI RCATGY 4 cut(s) 133, 224, 841, 1391
XmiI GTMKAC 2 cut(s) 1038, 1345
XmnI GAANNNNTTC 1 cut(s) 1162
XspI CTAG 3 cut(s) 582, 1026, 1313
ZrmI AGTACT 1 cut(s) 645
Zsp2I ATGCAT 2 cut(s) 6, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.